We narrowed to 17,478 results for: IGH@
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Plasmid#222142PurposeAdenine base editor with modified TadA variant for germline A-to-G base editing in zebrafishDepositorInsertModified TadA-nSpCas9 with nanos UTR
UseCRISPR; Zebrafish expressionTagsBipartite NLSPromoterSP6Available SinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
iRFP682-Smad2
Plasmid#118943PurposeMammalian expression of human Smad2 proteinDepositorAvailable SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX330_TOMM20 sgRNA / hSpCas9
Plasmid#172836PurposeMammalian expression of a sgRNA targeting the intron 4 (last intron) of TOMM20 (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 4 of TOMM20 under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR-CMV-TetO2_3C-Avi-His6_IRES-mRuby2
Plasmid#113889PurposeMammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic mRuby2DepositorTypeEmpty backboneUseLentiviralTags3C-Avi-His6PromoterCMV-MIE-TetO2Available SinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCH4 (denAsCas12a-KRAB lenti)
Plasmid#217331PurposeLentiviral expression of denAsCas12a-KRABDepositorInsertdenAsCas12a
UseCRISPR and LentiviralTags6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2MutationD908A/E174R/S542R/K548RPromoterUCOE-SFFVAvailable SinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-U6-gRNA-UbC-eGFP-P2A-Bsr
Plasmid#83925PurposeLentiviral SpCas9-gRNA expression vector with eGFP-P2A-BlastRDepositorInsertseGFP
Bsr
UseCRISPR and LentiviralTagsP2APromoterhUbCAvailable SinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-WHaloCaMP1a
Plasmid#205310PurposeProduction of AAV particles encoding WHaloCaMP1a from the CaMKII promoterDepositorInsertWHaloCaMP1a
UseAAVAvailable SinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAM4891 (pCV0003)
Plasmid#120080PurposeBroad host range plasmid. Bacteria plasmid vector to test various parts and devices. Expression of GFP and a Kanamycin/Neomycin resistance gene in various cyanobacteria.DepositorInsertPlasmid assembled from 3 CYANO-VECTOR modular devices: CV-RSF1010Y25F, CV-aph1, CV-PconII-LTRBS-GFPmut2
UseOtherMutationmobA Y25FAvailable SinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
vDip-C: pAAV-Pcp2-mGreenLantern-KASH
Plasmid#207613PurposevDip-C AAV vector with mouse Pcp2 (also known as L7) promoter driving green fluorophore mGreenLantern with a KASH nuclear membrane tag to isolate cell nuclei of cerebellar Purkinje cellsDepositorInsertmGreenLantern
UseAAVTagsKASHPromotermouse Pcp2 promoter (L7-6)Available SinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyso-ExRai-AktAR2
Plasmid#184052PurposeLysosome-targeted ExRai-AktAR2.DepositorInsertLyso-ExRai-AktAR2
TagsLysosome-associated membrane protein 1 (LAMP1).ExpressionMammalianPromoterCMVAvailable SinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GNAI1_G-alpha
Plasmid#110012PurposeProtein expression and purification of GNAI1_G-alphaDepositorInsertGNAI1_G-alpha (GNAI1 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-haA3A-CBE-A-NG
Plasmid#220895PurposeExpress haA3A-CBE-A-NG (with SpCas9-NG variant) in mammalian cellsDepositorInserthaA3A-CBE-A-NG
ExpressionMammalianAvailable SinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFlp-Gm
Plasmid#222335PurposeExpresses Flp and gentamicin to enable excision of antibiotic resistance cassettes; flp-cl-sacB-pL1V-Lv1-gent-pBBR1-ELT3DepositorAvailable SinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1 3xFlag-p38alpha
Plasmid#47575PurposeBacterial expression plasmid for GST-3xFlag-tagged p38alphaDepositorAvailable SinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pX330_TUBA1B sgRNA / hSpCas9
Plasmid#172834PurposeMammalian expression of a sgRNA targeting the intron 1 of TUBA1B (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 1 of TUBA1B under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZCS36 (Phsp16.41::Cas9(dpiRNA)::tbb-2 ‘3UTR)
Plasmid#193048PurposeHeatshock expressed Cas9DepositorInserthsp16.41p::Cas9(dpiRNA)::tbb-2 ‘3UTR
UseCRISPRExpressionWormPromoterhsp16.41pAvailable SinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRExT2A-NT-PmNG
Plasmid#232200PurposeEndogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: PAC-3xTy-T2A-3xTy-mNeonGreenDepositorInsertmNeongreen
ExpressionBacterialAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pENTR-JNKKTRmRuby2
Plasmid#59148PurposeORF encoding JNK KTR mRuby2 flanked by Gateway sequencesDepositorInsertJNK Kinase Translocation Reporter (MAPK8 Mouse, Human)
TagsmRuby2Available SinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) EGFP-RalA(WT)
Plasmid#224274PurposeCell transfection and expression of RalADepositorInsertRalA (RALA Human)
ExpressionMammalianAvailable SinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
PME-1 pcDNA-StrepIII
Plasmid#119292PurposeMammalian overexpression of StrepII-tagged full length PPME1DepositorInsertPPME1 (PPME1 Human)
ExpressionMammalianAvailable SinceJan. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX330_CALR sgRNA / hSpCas9
Plasmid#172838PurposeMammalian expression of a sgRNA targeting the intron 1 of CALR (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 1 of CALR under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
mTagRFP-T-LaminB1-10
Plasmid#58020PurposeLocalization: Nuclear Envelope, Excitation: 555, Emission: 584DepositorAvailable SinceNov. 4, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJL1-SpCas9
Plasmid#117051PurposeIn vitro expression of S. pyogenes Cas9 from the T7 promoterDepositorInsertS. pyogenes Cas9
ExpressionBacterialPromoterT7Available SinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
Ai34
Plasmid#34881DepositorInsertCAG-Floxed Synaptophysin-tdTomato
UseMouse TargetingTagstdTomatoPromoterCAGAvailable SinceMarch 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GNAQ_G-alpha
Plasmid#109809PurposeProtein expression and purification of GNAQ_G-alphaDepositorInsertGNAQ_G-alpha (GNAQ Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP - FLAG-APEX2- hs Lamin B1
Plasmid#139442PurposeAPEX2-Lamin B1 expression plasmidDepositorInsertLMNB1 (LMNB1 Human)
Tags3xGGGGS linker (between APEX2 and Lamin B1), APEX…ExpressionMammalianPromoterCMVAvailable SinceMay 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEL066
Plasmid#124897PurposeFor genome editing in plant by SpCas9 variant(SpCas9-NG) (D10A)-PmCDA1-UGI base editorDepositorTypeEmpty backboneUseCRISPRTags3xFlagExpressionPlantAvailable SinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCardinal-Lysosomes-20
Plasmid#56166PurposeLocalization: Lysosome Membrane, Excitation: 604, Emission: 659DepositorInsertLAMP1
TagsmCardinalExpressionMammalianPromoterCMVAvailable SinceFeb. 27, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3-N-mSOG-FRB-IRES-mCherry
Plasmid#136602PurposeEncodes FRB tagged with the N-terminal fragment of split-miniSOG (mSOG1-94).DepositorInsertN-mSOG-FRB
ExpressionMammalianPromoterCMVAvailable SinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-GA_AIO-GZV-SynZiFTR_mGL donor
Plasmid#229788PurposeBxb1-GA donor plasmid with downstream tandem syntax for all-in-one grazoprevir-inducible mGreenLantern expressionDepositorInsertmGreenLantern; ZF10-NS3-p65
UseSynthetic BiologyExpressionMammalianPromoterZF10 and CAGAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
YFP-S100A10
Plasmid#107200PurposeExpresses YFP-human S100A10 fusion protein for live cell imagingDepositorAvailable SinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSR651
Plasmid#65213PurposeBaculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-1 cap gene in Sf9 insect cellsDepositorInsertsmodified AAV-2 rep78
modified AAV-1 cap
UseAAVExpressionInsectPromoterp10 and polyhedrinAvailable SinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pXJ-Myc-KLF2
Plasmid#232654Purposetransient expresses KLF2 in mammalian cellsDepositorAvailable SinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
PAmCherry1-C1-LACT-C2
Plasmid#202682PurposePhotoactivatable lipid biosensorDepositorInsertPAmCherry1: Bos taurus MFGE8 (274-431) (MFGE8 Bovine)
ExpressionMammalianAvailable SinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
p3015b
Plasmid#209317PurposeExpresses a sgRNA template with a protospacer cloning site flanked by BsaI restriction sites for easy insertion of a targeting cassette.DepositorInsertGroup B Streptococcus-compatible single guide RNA
ExpressionBacterialPromoterXyl/tet promoter that is constitutively activeAvailable SinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-G3635A-L17-Q2L7-T2176N-UGI-Puro
Plasmid#209647PurposeTo install m.G3635 mutation on mtDNADepositorInsertL17-Q2L7-T2176N
ExpressionMammalianAvailable SinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
mTagRFP-T-Gamma-Tubulin-17
Plasmid#58017PurposeLocalization: Centrosomes, Excitation: 555, Emission: 584DepositorAvailable SinceJuly 31, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
mCerulean3-Mito-7
Plasmid#55433PurposeLocalization: Mitochondria, Excitation: 433, Emission: 475DepositorAvailable SinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMP0.4-FHS-paCaMKII(SD)-WPRE3
Plasmid#165430PurposeExpresses light-insensitive photoactivatable CaMKII(SD: Super Dark) in mammalian cells as a negative control.DepositorInsertFHS-paCaMKII(SD)
UseAAVTagsFHS(Flag, HisX6, Strep) tagExpressionMammalianMutationC450A, L514K, G528A, L531E, N538E: Light-insensit…PromoterCaMKII0.4Available SinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmiRFP703-Clathrin
Plasmid#79997PurposeClathrin labeling with near-infrared fluorescent protein miRFP703DepositorAvailable SinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSLIK 3xFLAG-CSE1L zeo
Plasmid#192320PurposeLentiviral expression vector for an inducible 3xFLAG-CSE1LDepositorAvailable SinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-mNeongreen-DARPinOFF7
Plasmid#182415PurposeBacterial expression of Vesicle Nucleating peptide-mNeongreen-DARPinOFF7 fusionDepositorInsertVNp-mNeongreen-DARPinOFF7
TagsVesicle Nucleating peptide (VNp) & mNeongreenExpressionBacterialPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
2xFKBP-mCh-KDEL
Plasmid#173010PurposeFKBP anchor for retaining DHFR-fused cargoes in the ER in the presence of zapalog. Encodes ER retrieval motif (KDEL) at C-terminus of mCherry. Contains IRES following ORF for cloning cargo.DepositorInsert2xFKBP-mCh-KDEL
TagsmCherryExpressionMammalianPromoterChicken beta actin (CAG)Available SinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAEL005635-Cas9
Plasmid#100608PurposeThe promoter of AAEL005635 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegyptiDepositorInsertsAAEL005635-hspcas9-GFP
Opie2-dsRed
UseCRISPRTagsGFP and dsREDExpressionInsectPromoterAAEL005635 and Opie2Available SinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCytERM-mBaoJin-N1
Plasmid#229567PurposeExpresses P450-mBaoJin fusion in mammalian cellsDepositorInsertmBaoJin
ExpressionMammalianAvailable SinceJuly 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRSETb-pcDronpa2-A69T
Plasmid#98578Purposephotoconvertible fluorescent protein pcDronpa2-A69T in pRSetB plasmid, photoconvertible by primed conversion mechanism, bacterial overexpressionDepositorInsertpcDronpa2-A69T
Tags6xHisExpressionBacterialPromoterT7Available SinceMarch 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mNeptune2
Plasmid#51309PurposeExpresses far-red fluorescent protein mNeptune2 in mammalian cellsDepositorInsertmNeptune2
ExpressionMammalianPromoterCMVAvailable SinceApril 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
PresentER-NLVPMVATV (GFP)
Plasmid#102947PurposePresentER minigene encoding HLA-A*02:01 ligand NLVPMVATV (GFP)DepositorInsertNLVPMVATV
UseRetroviralExpressionMammalianPromoterLTRAvailable SinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
VfAU1-LOV_P2A_casp9
Plasmid#131224PurposeExpresses Ha-tagged VfAU1-LOV and myc-tagged casp9 (domains of Opto-casp9) separated by P2A siteDepositorInsertVfAU1-LOV-HA_P2A_myc-casp9
ExpressionMammalianPromoterCMVAvailable SinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDFNKI-strepII-3C-LIC-strep
Plasmid#108708PurposeExpression of a StrepII-tagged target protein with 3C protease cleavage site.DepositorTypeEmpty backboneTagsStrepII-3C protease cleavage siteExpressionBacterialPromoterT7Available SinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only