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Showing: 9001 - 9040 of 161249 results

  3. pPP085

    Plasmid
    #158795
    Purpose
    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-2 in MCS1 for expression in E. coli and protein purification
    Depositor
    Jennifer Doudna
    Article
    Pausch et al Science. 2020 Jul 17;369(6501):333-337.
    Insert
    CasPhi-2
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    158795-plasmid-map-sequence-id-309510

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  4. pBbB8k-csg-nb112

    Plasmid
    #171702
    Purpose
    Arabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a nanobody targeting the spike protein of SARS-CoV-2 via a flexible linker.
    Depositor
    Neel Joshi
    Article
    Birnbaum et al ACS ES T Water. 2022 Nov 11;2(11):1836-1
    Insert
    csgBACEFG, with nanobody fused to CsgA
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171702-plasmid-map-sequence-id-336472

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  8. pOpen-P45

    Plasmid
    #165570
    Purpose
    Significantly improves maximum amplicon size and PCR yields for Family B polymerases like Pfu or Phusion. Comparable to ArchaeMaxx/PfuTurbo.
    Depositor
    FreeGenes Project, Jennifer Molloy, Drew Endy
    Insert
    P45
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 23, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165570-plasmid-map-sequence-id-340824

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  12. pKEK3394

    Plasmid
    #234078
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Article
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    tspurple
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pJ23100-BsaI
    Available since
    May 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    234078-plasmid-map-sequence-id-472134

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  13. 8061-E33

    Plasmid
    #234270
    Purpose
    Gateway ORF Entry clone of mouse Smad3 with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    Smad3 (Smad3 Mouse)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    234270-plasmid-map-sequence-id-468854

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  15. pKEK3238

    Plasmid
    #234092
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Article
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    sfCherry2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    E117Q/T127I/G219A
    Promoter
    pJ23100-BsaI
    Available since
    April 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    234092-plasmid-map-sequence-id-467988

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  16. pKEK3228

    Plasmid
    #234090
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Article
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    aeBlue
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pJ23100-BsaI
    Available since
    April 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    234090-plasmid-map-sequence-id-467993

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  17. pKEK3227

    Plasmid
    #234089
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Article
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    YukonOFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pJ23100-BsaI
    Available since
    April 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    234089-plasmid-map-sequence-id-467986

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  19. pAIP4_2EYI

    Plasmid
    #234173
    Purpose
    Heterologous protein expression of alpha-actinin-1 in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    2EYI (ACTN1 Human)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234173-plasmid-map-sequence-id-464138

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  23. pcDNA-SUN2 mApple

    Plasmid
    #187679
    Purpose
    In vivo visualization of SUN2
    Depositor
    Mary Beckerle
    Insert
    SUN2 (Sun2 Mouse)
    Use
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    CMV
    Available since
    Sept. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    187679-plasmid-map-sequence-id-371231

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  25. SEC14L3

    Plasmid
    #73240
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SEC14L3 (SEC14L3 Human)
    Use
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 25, 2016
    Availability
    Academic Institutions and Nonprofits only

  26. SEC14L4

    Plasmid
    #73233
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SEC14L4 (SEC14L4 Human)
    Use
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    73233-plasmid-map-sequence-id-137211

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  36. pITGA4-GFP

    Plasmid
    #81178
    Purpose
    Mammalian expression of human full length ITGA4-EGFP
    Depositor
    Chinten James Lim
    Insert
    ITGA4 (ITGA4 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Contains KLGFFKR motif; A1002L (please see deposi…
    Promoter
    CMV
    Available since
    Oct. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    81178-plasmid-map-sequence-id-175302

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  40. DD-Cas9 with filler sequence and Cre-ERT2 (EDCICE)

    Plasmid
    #90086
    Purpose
    This plasmid contains destabilized Cas9 and has Cre-ERT2 after IRES sequence. This vector also contains filler sequence which required to be removed for cloning of desired sgRNA
    Depositor
    Raffaella Sordella
    Article
    Senturk et al Nat Commun. 2017 Feb 22;8:14370. doi: 10
    Insert
    Cas9
    Use
    CRISPR and Lentiviral
    Tags
    Destabilized Domain and Flag
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    90086-plasmid-map-sequence-id-182687

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Showing: 9001 - 9040 of 161249 results