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173,387 results

Showing: 9001 - 9040 of 173387 results

  3. DD-Cas9 with filler sequence and Cre-ERT2 (EDCICE)

    Plasmid
    #90086
    Purpose
    This plasmid contains destabilized Cas9 and has Cre-ERT2 after IRES sequence. This vector also contains filler sequence which required to be removed for cloning of desired sgRNA
    Depositor
    Raffaella Sordella
    Article
    Senturk et al Nat Commun. 2017 Feb 22;8:14370. doi: 10
    Insert
    Cas9
    Use
    CRISPR and Lentiviral
    Tags
    Destabilized Domain and Flag
    Expression
    Mammalian
    Promoter
    EFS
    Available Since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    90086-plasmid-map-sequence-id-182687

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  4. mEmerald-MPO-C-18

    Plasmid
    #54186
    Purpose
    Localization: Lysosomes, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    MPO (MPO Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 10, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  10. AAV-CAG-hChR2-H134R-tdTomato (AAV Retrograde)

    Viral Prep
    #28017-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from AAV-CAG-hChR2-H134R-tdTomato (#28017). In addition to the viral particles, you will also receive purified AAV-CAG-hChR2-H134R-tdTomato plasmid DNA. Humanized channelrhodopsin H134R mutant fused to tdTomato, under the control of the CAG promoter. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karel Svoboda
    Article
    Mao et al Neuron. 2011 Oct 6;72(1):111-23.
    Promoter
    CAG
    Tags
    tdTomato
    Available Since
    June 20, 2017
    Availability
    Academic Institutions and Nonprofits only

  15. mCherry-TGNP-N-10

    Plasmid
    #55145
    Purpose
    Localization: Golgi Complex, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    TGNP (TGOLN2 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55145-plasmid-map-sequence-id-339930

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  21. EF1α-Lox2272-LoxP-MARKS·mTurquoise2-NLS·turboRFP-Lox2272-LoxP

    Plasmid
    #198753
    Purpose
    Cre reporter, which switches from membraneous mTurquoise to nuclear RFP upon Cre recombinase activity
    Depositor
    Jacco van Rheenen
    Article
    Vizoso et al Nat Commun. 2022 Oct 28;13(1):6442. doi:
    Insert
    EF1a promotor-Lox2272-LoxP-MARKS-mTurquoise2-NLS.turboRFP-Lox2272-LOXP
    Use
    Lentiviral
    Expression
    Bacterial and Mammalian
    Mutation
    See depositor comments below
    Promoter
    EF1a
    Available Since
    July 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    198753-plasmid-map-sequence-id-398000

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  31. pcDNA3-TLR3-CFP

    Plasmid
    #13641
    Depositor
    Doug Golenbock
    Insert
    Toll-Like Receptor 3 (TLR3 Human)
    Tags
    CFP
    Expression
    Mammalian
    Available Since
    June 12, 2007
    Availability
    Academic Institutions and Nonprofits only
    13641-plasmid-map-sequence-id-7719

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  36. pYPQAT57

    Plasmid
    #223429
    Purpose
    T-DNA vector for dSpCas9 mediated gene activation for dicot plants; NGG PAM; dSpCas9-Act3.0 was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    2x35s-dSpCas9-Act3.0-AtU3-gRNA scaffold 2.0
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223429-plasmid-map-sequence-id-453502

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  38. pcDNA C12 ecDHFR DD YFP HA

    Plasmid
    #192815
    Purpose
    Expresses an N-terminal enhanced destabilizing domain (DD) version of E. coli DHFR with higher basal turnover in mammalian systems. Contains missense mutations W74R/T113S/E120D/Q146L.
    Depositor
    John Hulleman
    Article
    Nakahara et al ACS Chem Biol. 2022 Sep 19. doi: 10.1021
    Insert
    E. coli dihydrofolate reductase
    Tags
    enhanced yellow fluorescent protein and hemagglut…
    Expression
    Mammalian
    Mutation
    W74R/T113S/E120D/Q146L
    Promoter
    CMV
    Available Since
    Nov. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    192815-plasmid-map-sequence-id-377240

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Showing: 9001 - 9040 of 173387 results