167,985 results

1. mouse E-cadherin GFP (423)

   Plasmid

   #67937

   Purpose

   Mammalian expression of E-cadherin-GFP

   Depositor

   Alpha Yap

   Article

   Truffi et al J Cell Sci. 2014 Jun 1;127(Pt 11):2420-3

   Insert

   E-cadherin (Cdh1 Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKS035 - pCAGGS-3XFLAG-Rad21-mKate2-bpA

   Plasmid

   #156445

   Purpose

   For transient expression of mouse RAD21 (SCC1) tagged with mKate2

   Depositor

   Elphege Nora

   Article

   Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

   Insert

   mouse RAD21 (Rad21 Mouse)
   

   Expression

   Mammalian

   Mutation

   none

   Available Since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-alpha-synuclein-BiFC

   Plasmid

   #87855

   Purpose

   pETDUET-1 based vector for BiMolecular Fluorescence Complementation based assays of alpha-synuclein oligomerisation

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

   Inserts

   alpha-synuclein-VC155
   alpha-synuclein-VN154
   

   Tags

   C-terminal fragment of mVenus BiFC protein and N-…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAGGS-synPCB2.1

   Plasmid

   #139478

   Purpose

   Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

   Depositor

   Kazuhiro Aoki

   Article

   Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

   Insert

   MTS-Fnr-Fd-P2A-MTS-PcyA-FLAG-P2A-MTS-HA-HO1
   

   Expression

   Mammalian

   Promoter

   CAG promoter

   Available Since

   April 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSET-mSA

   Plasmid

   #39860

   Depositor

   Sheldon Park

   Article

   Lim et al Biotechnol Bioeng. 2013 Jan;110(1):57-67

   Insert

   monomeric streptavidin
   

   Tags

   6xHis and FLAG

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFH2.22_mCherry-MS2x8

   Plasmid

   #205537

   Purpose

   Express mCherry-MS2x8 (export tag consisting of 8 tandem repeats of MS2 stem-loop aptamer)

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   mCherry
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pλPR cI-Elysis

   Plasmid

   #176891

   Purpose

   Bacterial expression plasmids for production of E. coli bacterial ghost (BG) capsules

   Depositor

   James Inglese

   Article

   Dranchak et al Dis Model Mech. 2023 Mar 1;16(3):dmm0498

   Insert

   Lysis E gene from PhiX174 and lambda PR-cI857 regulatory system
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. eMscL WT

   Plasmid

   #107454

   Purpose

   eMscL WT is optimized for mammalian rodent neuronal expression to the plasma membrane through a neuron-specific promoter and a voltage-gated channel targeting motif

   Depositor

   Francesco Difato

   Article

   Soloperto et al J Cell Sci. 2018 Jan 29. pii: jcs.210393

   Insert

   bacterial mechanosensitive ion channel of large conductance (mscL Escherichia Coli)
   

   Use

   Adeno-associated viral

   Tags

   Kir2.1 ER export signal (FCYENEV) and tdTomato fl…

   Expression

   Mammalian

   Promoter

   human synapsin 1

   Available Since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLX_lenti_osTir1_9Myc_P2A_Bsd

   Plasmid

   #139746

   Purpose

   lentiviral vector expressing rice E3 ligase Tir1 in mammalian cells with selection marker

   Depositor

   Robert Tjian

   Article

   Xie et al Nat Methods. 2020 Apr;17(4):430-436. doi

   Insert

   rice E3 ligase Tir1 (osTir1)
   

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-VEGFR2-V5/HIS

    Plasmid

    #205617

    Purpose

    Expression of human VEGFR2 receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    VEGFR2 receptor tyrosine kinase (KDR Human)
    

    Tags

    V5-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. HIV-1 LTR-gfp

    Plasmid

    #115809

    Purpose

    HIV-1 LTR driven reporter vector that retains complete LTRs, tat, and rev, but has a frameshift mutation in env, an ngfr reporter gene in place of nef, and gfp in place of gag, pol, vif, and vpr

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    gfp and ngfr
    

    Use

    Lentiviral

    Promoter

    HIV1 LTR

    Available Since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. mApple-C1

    Plasmid

    #54631

    Purpose

    Localization: C1 Cloning Vector, Excitation: 568, Emission: 592

    Depositor

    Michael Davidson

    Article

    Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

    Type

    Empty backbone

    Tags

    mApple

    Expression

    Mammalian

    Available Since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. MAC_N_PAX8

    Plasmid

    #167788

    Purpose

    Expresses PAX8 in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    PAX8 (PAX8 Human)
    

    Tags

    MAC

    Expression

    Mammalian

    Mutation

    no mutations

    Available Since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCXLE-Oct4-p2a-Klf4-IRES-Sox2-17

    Plasmid

    #193298

    Purpose

    episomal OKS* (expressing mouse Oct4 + Klf4 + super-Sox) for generation of integration-free iPSCs

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    Oct4-p2a-Klf4-IRES-Sox2-17 (Sox2, Pou5f1, Klf4 Mouse)
    

    Expression

    Mammalian

    Mutation

    Sox2-17 is engineered highly cooperative chimeric…

    Promoter

    CAG

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. PGK-AAVS1ZFNR

    Plasmid

    #60915

    Purpose

    encodes zinc finger nuclease specific to AAVS1 locus (right)

    Depositor

    Paul Gadue

    Article

    Sim et al Methods Mol Biol. 2015 Jan 29.

    Insert

    AAVS1-ZFNR
    

    Promoter

    PGK

    Available Since

    Feb. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. GPRC6A-DuET

    Plasmid

    #213299

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GPRC6A (GPRC6A Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. PIK3CA-WT

    Plasmid

    #16643

    Depositor

    Bert Vogelstein

    Article

    Samuels et al Science. 2004 Apr 23. 304(5670):554.

    Insert

    PIK3CA (PIK3CA Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    March 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. nls-mCherry

    Plasmid

    #108881

    Purpose

    Expression of nuclear localized mCherry

    Depositor

    Rob Parton

    Article

    Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

    Insert

    nls-mCherry
    

    Use

    Can be used for mammalian expression or in vitro …

    Expression

    Mammalian

    Available Since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. GST-AR-1-660

    Plasmid

    #104196

    Purpose

    Expresses human AR-1-660 from bacterial expression vector pGEX-5X-1 as GST fusion protein

    Depositor

    Elizabeth Wilson

    Article

    He et al J Biol Chem. 2000 Jul 28;275(30):22986-9

    Insert

    human androgen receptor aa 1-660 (AR Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSB1A3 EcoRI-RTX with EcoRI Methylase-AmilCP

    Plasmid

    #85165

    Purpose

    Provides the coding region to express the EcoRI restriction enzyme. The RTX tag allows the EcoRI enzyme to be isolated via calcium-precipitation.

    Depositor

    Robin Dowell

    Article

    Hendrix et al ACS Synth Biol. 2014 Dec 19;3(12):969-71

    Insert

    EcoRI-RTX and EcoRI Methylase-AmilCP coding sequences
    

    Tags

    AmilCP and RTX

    Expression

    Bacterial

    Promoter

    J23100 promoter

    Available Since

    Dec. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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21. pSUP-(2xCouRS-tRNAopt)

    Plasmid

    #197930

    Purpose

    Machinery plasmid expressing constitutive and inducible 7HCou synthetase and cognate amber suppressing tRNA. Incorporates (7-hydroxy-4-coumarin-yl) ethylglycine

    Depositor

    Kenneth Johnson

    Article

    Dangerfield et al J Biol Chem. 2020 Dec 11;295(50):17265-1

    Inserts

    CouRS
    tRNA opt
    

    Expression

    Bacterial

    Promoter

    glnS' and lambda(ts) and proK

    Available Since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40 (AAV1)

    Viral Prep

    #100054-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40 (#100054). In addition to the viral particles, you will also receive purified pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40 plasmid DNA. CAG-driven, humanized channelrhodopsin H134R mutant fused to mCherry for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Promoter

    CAG

    Tags

    mCherry

    Available Since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

23. ATG9A-mCherry

    Plasmid

    #197393

    Purpose

    Expresses ATG9A in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    De Pace et al PLoS Genet. 2018 Apr 26;14(4):e1007363.

    Insert

    ATG9A (ATG9A Human)
    

    Tags

    mCh

    Expression

    Mammalian

    Available Since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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24. pT3TS-RfxCas13d-NLS-HA

    Plasmid

    #141321

    Purpose

    Plasmid to carry out IVT of RfxCas13d-NLS (human codon-optimized)

    Depositor

    Ariel Bazzini, Miguel Angel Moreno-Mateos

    Article

    Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

    Insert

    Rfx-Cas13d (NLS-RfxCas13d-NLS)
    

    Use

    CRISPR

    Tags

    HA and NLS

    Available Since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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25. All-in-one lenti-ABE8e-NG

    Plasmid

    #200447

    Purpose

    Lentivirus vector for base editing

    Depositor

    Benjamin Cravatt

    Article

    Li et al Nat Chem Biol. 2023 Nov;19(11):1320-1330

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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26. pACEBac1-PARP2-Flag-His-WT

    Plasmid

    #111574

    Purpose

    Expresses full length PARP2 in Sf9 (insect) cells

    Depositor

    Thomas Muir

    Article

    Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

    Insert

    PARP2 (PARP2 Human)
    

    Tags

    C-terminal 6xHis and C-terminal Flag

    Expression

    Insect

    Promoter

    polyhedrin promoter

    Available Since

    July 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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27. pHR_Gal4UAS_humanIL-10_IRES_mCherry_PGK_tBFP

    Plasmid

    #85427

    Purpose

    Lentiviral vector for Gal4 inducible humanIL-10 with mCherry reporter and constitutive tBFP expression

    Depositor

    Wendell Lim

    Article

    Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

    Insert

    human IL-10
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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28. pCdrA-gfp(ASV)C

    Plasmid

    #111615

    Purpose

    Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species

    Depositor

    Tim Tolker-Nielsen

    Article

    Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

    Insert

    PcdrA-RBSII-gfp(ASV)-T0-T1
    

    Expression

    Bacterial

    Available Since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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29. pCMV-2E2-HA-mCh

    Plasmid

    #129596

    Purpose

    The encoded protein is the anti-HA frankenbody variant fused with the mCherry. It can be used to track mature and nascent HA tagged proteins in living organism.

    Depositor

    Tim Stasevich

    Article

    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

    Insert

    Anti-HA frankenbody variant-mCherry (2E2-HA scFv-mCherry)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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30. pCMV-scFv-GCN4-StayGold-IRESv4-HaloTag-tdMCP-CAAX

    Plasmid

    #241144

    Purpose

    Expresses Anti-GCN4 scFv-StayGold, HaloTag-tdMCP, and HaloTag-tdMCP-CAAX to track mature and nascent HA-tagged proteins and track MS2-tagged mRNA anchored to the plasma membrane

    Depositor

    Tim Stasevich

    Article

    Galindo et al Mol Biol Cell. 2025 Jun 1;36(6):mr6. doi

    Inserts

    Anti-GCN4 scFv
    tdMCP - CAAX
    

    Tags

    (n2)oxStayGold(c4) E147D and HaloTag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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31. SacI ML GFP Strand 11 Long

    Plasmid

    #164120

    Purpose

    Detect the long Mitochondria-Endoplasmic reticulum contact along with the OMMGFP1-10

    Depositor

    Tito Calì, Marisa Brini

    Article

    Cieri et al Cell Death Differ. 2018 Jun;25(6):1131-1

    Insert

    SacI ML GFP Strand 11 Long
    

    Expression

    Mammalian

    Available Since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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32. pME-mTagBFP

    Plasmid

    #75148

    Purpose

    TagBFP (blue) fluorophore for use with 5’ promoters.

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    mTagBFP
    

    Use

    Middle entry vector for multisite gateway three f…

    Promoter

    n/a

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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33. pVP91A-pcaHG

    Plasmid

    #113766

    Purpose

    Expresses the alpha (pcaG) and the beta (pcaH) subunits of Protocatechuate 3,4-Dioxygenase from Pseudomonas putida.

    Depositor

    John Lipscomb

    Article

    Knoot et al Proc Natl Acad Sci U S A. 2015 Jan 13;11

    Inserts

    β subunit of Protocatechuate 3,4- Dioxygenase
    α subunit of Protocatechuate 3,4- Dioxygenase
    

    Tags

    Met-6xHis

    Expression

    Bacterial

    Promoter

    T5 promoter

    Available Since

    Aug. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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34. AAVS1-TRE-U2AF2-TurboID-HA-rtTA

    Plasmid

    #226997

    Purpose

    Integrative plasmid to express U2AF2-TurboID in mammalian cells

    Depositor

    Bruno Dallagiovanna

    Article

    Gomes-Junior et al PLoS One. 2025 May 30;20(5):e0324779. do

    Insert

    U2 small nuclear RNA auxiliary factor 2 (U2AF2 Human)
    

    Tags

    TurboID-HA

    Expression

    Mammalian

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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35. lenti F9-TetR-EGFP-IRES-PuroR

    Plasmid

    #117049

    Purpose

    lentiviral vector for expression of TetR-EGFP, for visualization of TetO repeats

    Depositor

    Huimin Zhao

    Article

    Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

    Insert

    F9-TetR-EGFP
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    F9 promoter

    Available Since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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36. NC16 pCDNA3.1 FLAG NRF2

    Plasmid

    #36971

    Depositor

    Randall Moon

    Article

    Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

    Insert

    NRF2 (NFE2L2 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pFRT-TODestFLAGHA_HuB

    Plasmid

    #65755

    Purpose

    expresses FLAGHA-tagged HuB in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    ELAVL2 (ELAVL2 Human)
    

    Tags

    FLAG HA and V5

    Expression

    Mammalian

    Promoter

    CMV, 2x Tet

    Available Since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pGreenIIM RPS5A-mDII-ntdTomato/RPS5A-DII-n3Venus

    Plasmid

    #61629

    Purpose

    R2D2: Expresses a fusion of auxin-sensitive DII to nuclear 3xVenus and auxin-insensitive mDII to nuclear tdTomato, both from the Arabidopsis RPS5A promoter

    Depositor

    Dolf Weijers

    Article

    Liao et al Nat Methods. 2015 Mar;12(3):207-10. doi:

    Inserts

    mDII
    ntdTomato
    DII
    n3xVenus
    

    Expression

    Plant

    Promoter

    RPS5A

    Available Since

    March 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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39. gag-PE

    Plasmid

    #211371

    Purpose

    Packaging plasmid for v3 PE-eVLP production

    Depositor

    David Liu

    Article

    An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

    Insert

    MMLVgag-PE
    

    Expression

    Mammalian

    Available Since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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40. XEC

    Plasmid

    #233341

    Purpose

    expressing SARS-CoV-2 XEC spike protein for pseudovirus production

    Depositor

    David Nemazee

    Insert

    Spike (XEC) (S SARS-CoV-2)
    

    Expression

    Mammalian

    Mutation

    T22N, F59S on the basis of KP.3

    Promoter

    CMV

    Available Since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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