165,802 results

1. MSCV-Cas9-2A-BFP

   Plasmid

   #164662

   Purpose

   Retroviral introduction of Cas9 into mammalian cell line coupled to expression of blue fluorescent protein

   Depositor

   Daniel Hodson

   Article

   Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

   Inserts

   Cas9
   BFP
   

   Use

   CRISPR and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   MSSV_LTR

   Available Since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Ai6

   Plasmid

   #22798

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Nat Neurosci. 2010 Jan . 13(1):133-40.

   Insert

   CAG-Floxed ZsGreen
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX5X.1 PINK1 WT

   Plasmid

   #13321

   Depositor

   Mark Cookson

   Article

   Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

   Insert

   PINK1 kinase domain (PINK1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Amino acids 112-496

   Promoter

   Available Since

   Nov. 9, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPEgRNA

   Plasmid

   #172716

   Purpose

   Delivery of guide RNA for prime editing

   Depositor

   Tilmann Weber

   Article

   Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

   Insert

   gRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYPQ173

   Plasmid

   #99907

   Purpose

   Express CRISPR-Act2.0 system which contains pco-dCas9-VP64 fusion protein and MS2-VP64 fusion protein linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequence

   Depositor

   Yiping Qi

   Article

   Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

   Insert

   pco-dCas9-VP64-T2A-MS2-VP64
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-Ef1a-Con/Fon-oScarlet

   Plasmid

   #137136

   Purpose

   Intersectional viral expression of oScarlet in cells expressing both Cre AND Flp

   Depositor

   INTRSECT 2.0 Project, Karl Deisseroth

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Con/Fon-oScarlet
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   E95D

   Promoter

   EF1a

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pZT-AAVS1-L1

   Plasmid

   #52637

   Purpose

   TALEN-mediated gene editing at human PPP1R12C/AAVS1 locus

   Depositor

   Jizhong Zou, Mahendra Rao

   Article

   Luo et al Stem Cells Transl Med. 2014 May 15. pii:

   Insert

   Left AAVS1 TALEN
   

   Use

   TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-Flag-NPM1wt

   Plasmid

   #131810

   Purpose

   Expresses Flag-NPM1wildtype (human) in mammalian cells through transient transfection

   Depositor

   Mark Minden

   Article

   Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

   Insert

   NPM1 (NPM1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-TFEB-WT-MYC (#647)

   Plasmid

   #99955

   Purpose

   mammalian expression

   Depositor

   James Brugarolas

   Article

   Vega-Rubin-de-Celis et al Autophagy. 2017 Mar 4;13(3):464-472. doi

   Insert

   TFEB (TFEB Human)
   

   Use

   Tags

   6xHis and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. Fuw-dCas9-dead Tet1CD-P2A-BFP

    Plasmid

    #108246

    Purpose

    Lentiviral vector to express dCas9-dead Tet1CD-P2A-tagBFP

    Depositor

    Rudolf Jaenisch

    Article

    Liu et al Cell. 2018 Feb 8. pii: S0092-8674(18)300

    Insert

    dCas9-dead Tet1CD-HA-P2A-BFP
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    dCas9-D10A, dCas9-H840A, Tet1CD-H1672Y, Tet1CD-D1…

    Promoter

    Ubc

    Available Since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. 5'UTR CGG 99x FMR1-EGFP

    Plasmid

    #63091

    Purpose

    Contains expanded CGG repeats (99 repeats) within the 5'UTR of FMR1 as found in the Fragile X Tremor Ataxia Syndrome (FXTAS). These repeats are in frame with EGFP.

    Depositor

    Nicolas Charlet-Berguerand

    Insert

    expanded CGG repeats (99 repeats) within the 5'UTR of FMR1 (FMR1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. MG1655 ΔendA ΔrecA (DE3)

    Bacterial Strain

    #37854

    Depositor

    Kristala Prather

    Article

    Tseng et al Microb Cell Fact. 2010 Nov 27;9:96.

    Bacterial Resistance

    None

    Available Since

    Nov. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

13. GFP-CaM-uTEVp

    Plasmid

    #163032

    Purpose

    FLiCRE protease for neuronal expression

    Depositor

    Alice Ting

    Article

    Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

    Insert

    GFP-CaM-uTEVp
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. TFORF1808

    Plasmid

    #143849

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TBX18 (TBX18 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLX-EF1a_GFP

    Plasmid

    #221481

    Purpose

    GFP lentiviral overexpression

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAX APP 695

    Plasmid

    #30137

    Depositor

    Tracy Young-Pearse, Dennis Selkoe

    Article

    Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

    Insert

    Amyloid Precursor Protein (APP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    wild type splice variant 695

    Promoter

    Available Since

    Aug. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-45b-POLR2A-CTD-mCherry

    Plasmid

    #185011

    Purpose

    Bacterial Expression of 6xHis C-terminal domain of POLR2A mCherry Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

    Insert

    POLR2A (Polr2a Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBT372

    Plasmid

    #125613

    Purpose

    Expresses evoCDA1-BE4max-NG in mammalian cells

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    evoCDA1-BE4max-NG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    F23S A123V I195F

    Promoter

    Available Since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. GFP-α-cateninWT

    Plasmid

    #229709

    Purpose

    Transient expression of GFP-alpha-cateninWT in mammalian cells

    Depositor

    Alpha Yap

    Article

    Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

    Insert

    CTNNA1 (alpha-catenin) Human (CTNNA1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. 783-Rx-hU6: RfxCas13d gRNA and array cloning backbone

    Plasmid

    #228360

    Purpose

    hU6-driven expression of RfxCas13d gRNAs and arrays compatible. Contains AarI sites for guide cloning flanked by 5' DR36

    Depositor

    Michael Boettcher

    Article

    El Kassem et al Nat Commun. 2025 Feb 14;16(1):1631. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral; Rfxcas13d grna expression …

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. AAVS1 CAG rtTA3 TauP301L 2N4R-EGFP

    Plasmid

    #132393

    Purpose

    Exchange cassette containing inducible TauP301L 2N4R-EGFP fusion, CAG-driven rtTA3, Puromycin resistance marker, targets AAVS1 site

    Depositor

    Gerold Schmitt-Ulms

    Article

    Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

    Insert

    human TauP301L 2N4R-EGFP (MAPT Synthetic, Human)
    

    Use

    CRISPR and Cre/Lox

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Proline 301 to Leucine (P301L)

    Promoter

    TREtight

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. AAV1 for Split-PE, SpCas9 nickase (PKC1001)

    Plasmid

    #190111

    Purpose

    AAV1 for expression of nickase SpCas9(H840A) for Split-PE use

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

    Insert

    nSpCas9
    

    Use

    AAV

    Tags

    bpNLS

    Expression

    Mutation

    H840A

    Promoter

    EF1a

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. OMM-SPLICSs-nFAST

    Plasmid

    #210430

    Purpose

    Expresses outer mitochondria-targeted split-NFAST for Short-range ER-Mito Contact sites

    Depositor

    Tito Calì

    Insert

    OMM-SPLICSs-nFAST
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pHR-SFFV-dCas9-BFP

    Plasmid

    #46910

    Purpose

    Human expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS and tagBFP

    Depositor

    Jonathan Weissman, Stanley Qi

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Insert

    dCas9-BFP fusion
    

    Use

    CRISPR and Lentiviral

    Tags

    2xNLS, BFP, and HA

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available Since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pRK5-A3-HA-ubiquitin

    Plasmid

    #220145

    Purpose

    Expresses A3-Ub for E3 substrate identification by E-STUB

    Depositor

    William Sellers

    Article

    Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    A3-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. mTurquoise2

    Plasmid

    #198196

    Purpose

    Spectral correction: Expression of mTurquoise2

    Depositor

    Christian Wahl-Schott

    Article

    Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

    Insert

    mTurquoise2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A206K

    Promoter

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. CMV-eGFP-Z1

    Plasmid

    #135049

    Purpose

    Enhanced Green fluorescent protein expression for mammalian cells under constitutive CMV Promoter in minimal backbone plasmid.

    Depositor

    Jordan Green

    Insert

    eGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Promoter

    Available Since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3β-FLAG-CBP-HA

    Plasmid

    #32908

    Depositor

    Tso-Pang Yao

    Article

    Zhao et al Mol Cell Biol. 2005 Oct;25(19):8456-64.

    Insert

    CBP (Crebbp Mouse)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET21-pelB-LaG94-5

    Plasmid

    #228965

    Purpose

    For bacterial expression of anti-GFP nanobody LaG94-5, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaG94-5
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Nrp1-Fc-His

    Plasmid

    #72097

    Purpose

    Expresses the extracellular region of the Neuropilin 1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Nrp1 (Nrp1 Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCAS

    Plasmid

    #60847

    Purpose

    Expresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeast

    Depositor

    Jamie Cate

    Article

    Ryan et al Elife. 2014 Aug 19;3. doi: 10.7554/eLife

    Inserts

    S. pyogenese Cas9
    RNA pol III promoter (tRNA-Tyr)
    hepatitis delta virus ribozyme, genomic
    sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    NLS/His8 and TTT 3' extension prior to sgRNA

    Expression

    Bacterial and Yeast

    Mutation

    L4 is UUCG tetraloop and guide targets LYP1 (CATA…

    Promoter

    Available Since

    Jan. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3.1 MCS-BirA(R118G)-HA

    Plasmid

    #36047

    Purpose

    To fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); HA tag

    Depositor

    Kyle Roux

    Article

    Roux et al J Cell Biol. 2012 Mar 12.

    Has Service

    Cloning Grade DNA

    Insert

    BirA(R118G)-HA
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    R118G (highly promiscuous form)

    Promoter

    Available Since

    April 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pGWB512

    Plasmid

    #74854

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with FLAG (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available Since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCMV-SpCas9(H840A)-T2A-EGFP

    Plasmid

    #221231

    Purpose

    Express nCas9 nickase (H840A) with GFP reporter

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    SpCas9(H840A)-T2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H840A

    Promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLVX-Puro-TDP-43-A315T

    Plasmid

    #133755

    Purpose

    Lentiviral expression of human TDP-43 A315T disease mutant in mammalian cells.

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

    Insert

    TARDBP (TARDBP Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Changed alanine 315 to threonine

    Promoter

    CMV

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET-Sfp R4-4 mutant

    Plasmid

    #173491

    Purpose

    Expression plasmid for mutant R4-4 of Sfp phosphopantetheinyl transferase. R4-4 can use 3′-dephospho CoA for adding the phosphopantetheinyl group to the carrier proteins.

    Depositor

    Jun Yin

    Article

    Sunbul et al J Mol Biol. 2009 Apr 10;387(4):883-98. d

    Insert

    Sfp-R44
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. AAVS1-Pur-CAG-hM3Dq-mCherry

    Plasmid

    #80948

    Purpose

    human AAVS1 site targeting donor plasmid for knock-in hM3Dq-mCherry expression cassette driven by CAG promoter

    Depositor

    Su-Chun Zhang

    Article

    Chen et al Cell Stem Cell. 2016 Jun 2;18(6):817-26.

    Insert

    hM3Dq-mCherry
    

    Use

    Tags

    mcherry fusion

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available Since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. IKK1-EYFP

    Plasmid

    #111206

    Purpose

    fluorescent fusion protein

    Depositor

    Johannes A. Schmid

    Insert

    IKKalpha (CHUK Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLenti CMV/TO Hygro empty (w214-1)

    Plasmid

    #17484

    Purpose

    3rd gen lentiviral empty control vector, CMV/TO promoter, Hygro 

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. PB-tetO-NGN2

    Plasmid

    #197090

    Purpose

    PiggyBac plasmid with tet-inducible expression of transcription factors for cortical neuron differentiation

    Depositor

    Brian Wainger

    Article

    Held et al Cell Rep. 2023 Aug 30;42(9):113046. doi:

    Insert

    NGN2 (NEUROG2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge