167,948 results

1. pCDH-EF1-DIO-tdTomato

   Plasmid

   #72254

   Purpose

   Express tdTomato under EF-1 promoter when Cre is expressed by another vector

   Depositor

   Kazuhiro Oka

   Insert

   tdTomato
   

   Use

   Cre/Lox and Lentiviral

   Expression

   Mammalian

   Promoter

   elongation factor-1

   Available Since

   Jan. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-EF1a-DIO-JEDI-2P-WPRE (AAV1)

   Viral Prep

   #179460-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV-EF1a-DIO-JEDI-2P-WPRE (#179460). In addition to the viral particles, you will also receive purified pAAV-EF1a-DIO-JEDI-2P-WPRE plasmid DNA. EF1a-driven, Cre-dependent expression of voltage indicator JEDI-2P. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Francois St-Pierre

   Article

   Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

   Promoter

   EF1a

   Tags

   cEGFP (Cre-dependent)

   Available Since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

3. pCMV-SpRYc-ABE8e-EGFP

   Plasmid

   #208340

   Purpose

   Expresses SpRYc-ABE8e in mammalian cells

   Depositor

   Pranam Chatterjee

   Article

   Zhao et al Nat Commun. 2023 Oct 4;14(1):6175. doi:

   Insert

   ecTadA(8e)-nSpRYc
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Sc++ (1-1119), SpRY (1111-1368), D10A

   Promoter

   CMV

   Available Since

   Aug. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3 HA E2F1

   Plasmid

   #236431

   Purpose

   transient overexpression of E2F1 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

   Insert

   E2F1 (E2F1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   May 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. cfSGFP2-col4a1

   Plasmid

   #160317

   Purpose

   cfSGFP2-tagged collagen4a1 for mammalian expression

   Depositor

   Ikuo Wada

   Article

   Matsui et al Cell Struct Funct. 2020 Jul 23;45(2):107

   Insert

   COL4A1 (COL4A1 Human)
   

   Tags

   cfSGFP2

   Expression

   Mammalian

   Available Since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMXs-mHRI-FLAG-IP

   Plasmid

   #101791

   Purpose

   Retroviral vector encoding C-terminally FLAG tagged mouse HRI

   Depositor

   Seiichi Oyadomari

   Article

   Taniuchi et al Sci Rep. 2016 Sep 16;6:32886. doi: 10.10

   Insert

   Hri (Eif2ak1 Mouse)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   LTR

   Available Since

   Oct. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGP-AAV-syn-FLEX-jGCaMP8f-WPRE

   Plasmid

   #162379

   Purpose

   AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expression

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Has Service

   AAV Retrograde, AAV1, AAV5, and AAV9

   Insert

   jGCaMP8f
   

   Use

   AAV and Cre/Lox

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Q315L

   Promoter

   Synapsin

   Available Since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJEP314-pAAV-U6SaCas9gRNA(SapI)-CMV-SaCas9-DIO-pA

   Plasmid

   #113691

   Purpose

   U6 driven SaCas9 gRNA expression cassette followed by a CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Insert

   SaCas9
   

   Use

   AAV, CRISPR, and Cre/Lox

   Tags

   NLS

   Promoter

   Cytomegalo Virus(CMV)

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. psPAX2-D64V-NC-MS2

   Plasmid

   #122944

   Purpose

   MS2 coat protein-modified lentiviral packaging plasmid for packaging mRNA with an MS2 aptamer into lentivirus-like particles

   Depositor

   Baisong Lu

   Article

   Lu et al Nucleic Acids Res. 2019 Feb 13. pii: 531

   Insert

   NC-MCP (MS2 coat protein) fusion protein in Gag
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   D64V

   Available Since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. SacI ML GFP Strand 11 Short

    Plasmid

    #164121

    Purpose

    Detect the short Mitochondria-Endoplasmic reticulum contact along with the OMMGFP1-10

    Depositor

    Tito Calì, Marisa Brini

    Article

    Cieri et al Cell Death Differ. 2018 Jun;25(6):1131-1

    Insert

    SacI ML GFP Strand 11 Short
    

    Expression

    Mammalian

    Available Since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. GPR161-Tango

    Plasmid

    #66334

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPR161 (GPR161 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pAAV FLEX Synaptophysin GFP

    Plasmid

    #137188

    Purpose

    Cre-dependent expression of synaptophysin-GFP

    Depositor

    Matthew Nolan

    Article

    Surmeli et al Neuron. 2016 Nov 23;92(4):929. doi: 10.1

    Insert

    Synaptophysin GFP (Syp Mouse)
    

    Use

    AAV

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. ptf-Galectin3

    Plasmid

    #64149

    Purpose

    Expression in mammalian cells

    Depositor

    Tamotsu Yoshimori

    Article

    Maejima et al EMBO J. 2013 Aug 28;32(17):2336-47. doi:

    Insert

    Galectin3 (LGALS3 Human)
    

    Tags

    EGFP and mRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. MutaT7

    Bacterial Strain

    #156460

    Purpose

    An E. coli strain that expresses MutaT7 for mutagenizing genes downstream from a T7 promoter.

    Depositor

    Matthew D. Shoulders

    Article

    Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

    Bacterial Resistance

    Streptomycin

    Species

    Rattus norvegicus

    Available Since

    Oct. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3.1-HA-CDK4

    Plasmid

    #172612

    Purpose

    Expresses HA-tagged CDK4 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CDK4 (CDK4 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMV-SB100X[EF1α-copGFP]

    Plasmid

    #218323

    Purpose

    Donor plasmid of SB100X Transposon with copGFP mammalian expression cassette

    Depositor

    Haoyi Wang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    copGFP mammalian expression cassette
    

    Expression

    Mammalian

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. COX8mls-Flag-APEX2-SOPP3

    Plasmid

    #229075

    Purpose

    Expresses APEX2-SOPP3 in the mitochondrial matrix

    Depositor

    Weijun Pan

    Article

    Qu et al Cell Res. 2025 Feb;35(2):149-152. doi: 1

    Insert

    Express APEX2-SOPP3 in the mitochondrial matrix (APEX2 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET-45b(+)-Tn5

    Plasmid

    #112112

    Purpose

    Expresses hyperactive Tn5 transposase for next generation sequencing library preparation

    Depositor

    Frank Pugh

    Article

    Rossi et al Nat Commun. 2018 Jul 20;9(1):2842. doi:

    Insert

    Tn5
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    E54K E110K P242A L372P

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRK5-HA-SLC38A9.1

    Plasmid

    #71869

    Purpose

    overexpression

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Jan 9;347(6218):188-94. do

    Insert

    SLC38A9.1 (SLC38A9 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    CMV

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

20. PB-SAM-Dest

    Plasmid

    #102563

    Purpose

    Piggybac transposon DEST vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex. It is used for insertion of multiple U6-sgRNA expression cassettes.

    Depositor

    Ying Liu

    Article

    Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64

    Type

    Empty backbone

    Use

    CRISPR; Piggybac transposon

    Expression

    Mammalian

    Promoter

    N/A

    Available Since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. TFORF3311

    Plasmid

    #144787

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    CREB3 (CREB3 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV-pCMV-TM-KA2-CaM-NES-TevN-AsLOV2-TEVseq-tTA

    Plasmid

    #171617

    Purpose

    AAV Soma-targeted Cal-Light with KA2

    Depositor

    Hyungbae Kwon

    Article

    Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

    Insert

    TM-KA2-CaM-NES-TevN-AsLOV2-TEVseq-tTA
    

    Use

    AAV and Synthetic Biology

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHMattR3-attR4-GFP

    Plasmid

    #196247

    Purpose

    pHM1 derived plasmid containing the Gateway cassette attR3-attR4 and GFP gene

    Depositor

    Bing Yang

    Article

    Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3-ClopHensor

    Plasmid

    #25938

    Depositor

    Daniele Arosio

    Article

    Arosio et al Nat Methods. 2010 Jul . 7(7):516-8.

    Insert

    ClopHensor
    

    Tags

    Strep-II

    Expression

    Mammalian

    Mutation

    E2GFP is EGFP-T203Y

    Available Since

    Sept. 13, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. MSCV-human Igf2bp2-IRES-GFP

    Plasmid

    #91890

    Purpose

    Retroviral expression of human Igfbp2

    Depositor

    Martine Roussel

    Article

    Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

    Insert

    Igf2bp2 (IGF2BP2 Human)
    

    Use

    Retroviral

    Tags

    IRES-EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. SpCas9-HF1

    Plasmid

    #138556

    Purpose

    Expresses human codon-optimized high-fidelity SpCas9 and blasticidin resistance: EFS promoter-SpCas9-HF1-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    SpCas9-HF1
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, Q926A

    Promoter

    EFS

    Available Since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDONR223_SHC1_WT_V5

    Plasmid

    #82931

    Purpose

    Gateway Donor vector containing SHC1, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Matthew Meyerson, Jesse Boehm

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    SHC1 (SHC1 Human)
    

    Use

    Gateway entry vector

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-CamKII-Cre

    Plasmid

    #182736

    Purpose

    Cre expression under the control of CamKII promoter

    Depositor

    Janelia Viral Tools

    Insert

    Cre
    

    Use

    AAV

    Promoter

    CamKII

    Available Since

    April 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Construct 28 -hUBCp_TetR_p2a_LacI_p2a_ZeoR

    Plasmid

    #81253

    Purpose

    Lentiviral construct for building stable cell lines expressing TetR and LacI via Zeocin selection

    Depositor

    Timothy Lu

    Article

    Perli et al Science. 2016 Aug 18. pii: aag0511.

    Insert

    TetR and LacI
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pTKRED

    Plasmid

    #41062

    Depositor

    Thomas Kuhlman, Edward Cox

    Article

    Kuhlman et al Nucleic Acids Res. 2010 Apr;38(6):e92. E

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Feb. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pGolt-mCherry / pGolt3-mCherry

    Plasmid

    #73297

    Purpose

    Visualization of Dendritic Golgi Satellite

    Depositor

    Michael Kreutz

    Article

    Mikhaylova et al Cell Rep. 2015 Dec 30. pii: S2211-1247(1

    Inserts

    ER export signal of Scap
    ER export signal of Scap
    TMD (trans-membrane domain) of Calneuron-2 (Cabp7 Rat)
    

    Tags

    ER export and mCherry

    Expression

    Mammalian

    Promoter

    CMV immediate early promoter

    Available Since

    Feb. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Cyto-V5-APEX2

    Plasmid

    #170572

    Purpose

    Expresses APEX2 in cytosol

    Depositor

    James Olzmann

    Article

    Bersuker et al Dev Cell. 2018 Jan 8;44(1):97-112.e7. do

    Insert

    APEX2
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLacO-pEF1α-RFP

    Plasmid

    #179507

    Purpose

    Expresses monomeric RFP in mammalian cells under a EF1α promoter

    Depositor

    Yasushi Hiraoka

    Article

    Haraguchi et al Commun Biol. 2022 Jan 20;5(1):78. doi: 1

    Insert

    monomeric RFP
    

    Expression

    Mammalian

    Promoter

    EF1α

    Available Since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. mU6-sgRNA-CLTA-mCherry

    Plasmid

    #240637

    Purpose

    Vector for expressing an sgRNA targeting CLTA promoter from the mouse U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter.

    Depositor

    James Nuñez

    Article

    Xu et al Nat Commun. 2025 Aug 26;16(1):7948. doi:

    Insert

    puro-T2A-mCherry
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pHDR-USR-SpCas9

    Plasmid

    #208829

    Purpose

    HDR Universal Surrogate Reporter with a sgRNA and consistently expressed SpCas9. The PuroR reporter gene was designed to be repaired as a selecting marker gene only by the sgRNA/Cas9-triggered HDR.

    Depositor

    Zhiying Zhang

    Article

    Yan et al Mol Ther Nucleic Acids. 2020 Mar 6;19:77

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA5/FRT/TO_CalR-SNAP-tag2-KDEL

    Plasmid

    #226517

    Purpose

    Mamalian cell expression of SNAP-tag2 in endoplasmic reticulum

    Depositor

    Kai Johnsson

    Article

    Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

    Insert

    CalR-SNAP-tag2-KDEL
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pNF02-mLemon

    Plasmid

    #219399

    Purpose

    Constitutive expression of mLemon in E. coli

    Depositor

    Christian Lesterlin

    Article

    Fraikin et al Sci Adv. 2025 Apr 18;11(16):eads6201. do

    Insert

    mLemon
    

    Expression

    Bacterial

    Promoter

    proDp

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAAV-EF1α-DIO-SN

    Plasmid

    #181741

    Purpose

    AAV vector for expressing SN

    Depositor

    Yasunori Hayashi

    Article

    Goto et al Science. 2021 Nov 12;374(6569):857-863.

    Insert

    SuperNova (monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. AAV SYN flex PSAM4 GlyR IRES EGFP

    Plasmid

    #119741

    Purpose

    Cre-dependent chemogenetic inhibitor expression

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2019 Mar 14. pii: science.aav52

    Has Service

    AAV5 and AAV9

    Insert

    PSAM4 GlyR IRES eGFP (CHRNA7 Human, Synthetic)
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    L131G, Q139L, Y217F

    Promoter

    synapsin

    Available Since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pEMS2159

    Plasmid

    #70120

    Purpose

    Promoterless AAV plasmid with MCS to insert promoter for expression of iCre. Contains WPRE.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    ssAAV-MCS-iCre-WPRE
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    May 12, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge