Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

179,463 results

Showing: 1151 - 1200 of 179463 results

  3. pAAV-CaMKIIa-mCherry (AAV5)

    Viral prep
    #114469-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-CaMKIIa-mCherry (#114469). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-mCherry plasmid DNA. CamKIIa-driven expression of mCherry. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    CaMKII
    Tags
    mCherry
    Available since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only

  5. pAAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine (AAV8)

    Viral prep
    #50454-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine (#50454). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine plasmid DNA. Synapsin-driven, Cre-dependent, excitatory hM3D DREADD expression, with physically separate mCitrine expression for cell body labeling. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    mCitrine (Cre-dependent)
    Available since
    Sept. 20, 2018
    Availability
    Academic Institutions and Nonprofits only

  6. pAAV.CMV.PI.EGFP.WPRE.bGH (AAV8)

    Viral prep
    #105530-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV.CMV.PI.EGFP.WPRE.bGH (#105530). In addition to the viral particles, you will also receive purified pAAV.CMV.PI.EGFP.WPRE.bGH plasmid DNA. CMV-driven EGFP control. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    CMV
    Tags
    EGFP
    Available since
    June 29, 2018
    Availability
    Academic Institutions and Nonprofits only

  7. APEX2-OMM

    Plasmid
    #79056
    Purpose
    Soybean APEX2 anchored to cytosolic face of outer mitochondrial membrane with C-terminal targeting sequence of last 31aa of human mitochondrial antiviral-signaling protein
    Depositor
    Alice Ting
    Publication
    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n
    Insert
    Soybean APEX2
    Use
    Lentiviral
    Tags
    flag and last 31 amino acids of human MAVS
    Expression
    Bacterial and Mammalian
    Promoter
    CMV immearly promotor
    Available since
    Aug. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    79056-plasmid-map-sequence-id-238450

    Enlarge

  23. pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA (AAV Retrograde)

    Viral prep
    #20297-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA (#20297). In addition to the viral particles, you will also receive purified pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA plasmid DNA. Humanized channelrhodopsin H134R mutant fused to mCherry, driven by the EF1a promoter. Cre-dependent. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    EF1a
    Tags
    mCherry (Cre-dependent)
    Available since
    Aug. 22, 2017
    Availability
    Academic Institutions and Nonprofits only

  24. pML107

    Plasmid
    #67639
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformation
    Depositor
    John Wyrick
    Publication
    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1
    Inserts
    Cas9
    single guide RNA expression cassette
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    pSNR52 and pTDH3 (aka GAP promoter)
    Available since
    Aug. 31, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    8 Articles
    67639-plasmid-map-sequence-id-220324

    Enlarge

  29. pAAV-hSyn-hM4D(Gi)-mCherry

    Plasmid
    #50475
    Purpose
    Gi-coupled hM4D DREADD fused with mCherry under the control of human synapsin promoter.
    Depositor
    Bryan Roth
    Has service
    AAV1, AAV2, AAV5, AAV8, AAV9, and AAV Retrograde
    Insert
    hM4D(Gi)-mCherry
    Use
    AAV
    Tags
    mCherry
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    human Synapsin 1
    Available since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    118 Articles
    50475-plasmid-map-sequence-id-171072

    Enlarge

  30. pHIV-Luciferase

    Plasmid
    #21375
    Purpose
    self-inactivating 3rd generation lentiviral plasmid for co-expression of your gene of interest and Luciferase
    Depositor
    Bryan Welm
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Available since
    June 19, 2009
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    32 Articles
    21375-plasmid-map-sequence-id-357945

    Enlarge

  36. pAAV-Ef1a-DIO eNpHR 3.0-EYFP

    Plasmid
    #26966
    Purpose
    AAV expression of eNpHR 3.0 fused to EYFP driven by EF1a promoter for optogenetic inhibition
    Depositor
    Karl Deisseroth
    Publication
    Gradinaru et al Cell. 2010 Apr 2. 141(1):154-65.
    Has service
    AAV1, AAV5, AAV9, and AAV Retrograde
    Insert
    eNpHR 3.0
    Use
    AAV
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    ER export at 3' end of gene. Trafficking sig…
    Promoter
    EF1alpha
    Available since
    Jan. 27, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    89 Articles
    26966-plasmid-map-sequence-id-200278

    Enlarge

  40. pDest-565

    Plasmid
    #11520
    Purpose
    Gateway destination vector for bacterial expression; His,GST tag
    Depositor
    Dominic Esposito
    Type
    Empty backbone
    Use
    Gateway Cloning
    Tags
    6xHis and GST
    Expression
    Bacterial
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available since
    May 24, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    9 Articles
    11520-plasmid-map-sequence-id-324017

    Enlarge

  43. pLVX-EF1alpha-SARS-CoV-2-N-2xStrep-IRES-Puro

    Plasmid
    #141391
    Purpose
    Lentiviral expression of SARS-CoV-2 nucleocapsid (N) protein; transient expression and generate lentivirus
    Depositor
    Nevan Krogan
    Publication
    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:
    Insert
    SARS-CoV-2-N (N SARS-CoV-2)
    Use
    Lentiviral
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    human codon optimized
    Promoter
    EF1alpha
    Available since
    April 8, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    28 Articles
    141391-plasmid-map-sequence-id-288088

    Enlarge

  45. pAAV.Syn.Flex.GCaMP6f.WPRE.SV40 (AAV9)

    Viral prep
    #100833-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV.Syn.Flex.GCaMP6f.WPRE.SV40 (#100833). In addition to the viral particles, you will also receive purified pAAV.Syn.Flex.GCaMP6f.WPRE.SV40 plasmid DNA. Syn-driven, Cre-dependent GCaMP6f calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Publication
    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d
    Promoter
    Syn
    Available since
    May 14, 2018
    Availability
    Academic Institutions and Nonprofits only

  49. pdCas9

    Plasmid
    #46569
    Purpose
    Expresses the tracrRNA, the dCas9 catalytic site mutant and a CRISPR array designed for the easy cloning of new spacers.
    Depositor
    Luciano Marraffini
    Publication
    Bikard et al Nucleic Acids Res. 2013 Jun 12.
    Inserts
    tracrRNA
    dcas9
    CRISPR array
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Available since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    18 Articles
    46569-plasmid-map-sequence-id-364492

    Enlarge

Showing: 1151 - 1200 of 179463 results