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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,326 results for: ada

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Showing: 9841 - 9880 of 10326 results

  4. lentiSAM v2 (Puro)

    Plasmid
    #92062
    Purpose
    Modified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Adam Karpf
    Article
    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 (sgRNA) and EF1a (dCas9-VP64)
    Available Since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    92062-plasmid-map-sequence-id-180159

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  12. AXBEv2

    Plasmid
    #204606
    Purpose
    Mammalian expression of evolved adenine transversion base editor AXBEv2
    Depositor
    Dali Li
    Article
    Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103
    Insert
    bpNLS-TadA-8e-nSpCas9-mAAG (R165E/Y179F)-bpNLS-P2A-GFP
    Use
    CRISPR
    Tags
    6*His
    Expression
    Mammalian
    Mutation
    mAAG (R165E/Y179F); S. pyogenes Cas9 (D10A)
    Available Since
    Aug. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    204606-plasmid-map-sequence-id-404720

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  20. SpRYc

    Plasmid
    #175575
    Purpose
    Expresses the codon-optimized, engineered SpRYc Cas9 nuclease in mammalian cells
    Depositor
    Joseph Jacobson
    Article
    Koseki et al Res Sq. 2023 Mar 7:rs.3.rs-2625838. doi:
    Insert
    SpRYc
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    PID of SpRY grafted onto the N-terminal domain of…
    Promoter
    CMV
    Available Since
    May 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    175575-plasmid-map-sequence-id-345065

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  21. pRN3P_T3_ABE8e_IVT

    Plasmid
    #201676
    Purpose
    Plasmid to be used as DNA template for in vitro RNA transcription of the ABE8e base editor (A to G) by T3 RNA polymerase. The plasmid contains optimised 5'UTR and 3'UTR to improve protein expression.
    Depositor
    Timo Otonkoski
    Article
    Jalil et al Am J Hum Genet. 2024 Apr 4;111(4):714-72
    Insert
    ecTadA(8e)-nSpCas9
    Use
    CRISPR; Vector for in-vitro transcription
    Promoter
    T3 promoter
    Available Since
    April 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    201676-plasmid-map-sequence-id-406944

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  23. pFBP_2xNbLYS1::LUZ

    Plasmid
    #189918
    Purpose
    Binary vector for Agrobacterium-mediated transient expression of a bioluminescent reporter for assessing immune activation in Nicotiana benthamiana by transiently expressed plant immune receptors.
    Depositor
    Adam Steinbrenner
    Article
    Garcia et al Mol Plant Microbe Interact. 2023 Mar;36(
    Insert
    pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtUbi1:NnH3H:tAtuNos, pCmYLCV:NnHisps:tHsp, p2xNbLYS1:NnLuz:tAtuOcs
    Use
    Luciferase
    Expression
    Plant
    Promoter
    Various
    Available Since
    Nov. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    189918-plasmid-map-sequence-id-377067

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  24. pMAL-SH3(2)-5R

    Plasmid
    #112089
    Purpose
    Bacterial expression plasmid containing His and MBP tags for 5 repeats of the second SH3 domain from human NCK1.
    Depositor
    Michael Rosen
    Article
    Li et al Nature. 2012 Mar 7;483(7389):336-40. doi
    Insert
    SH3(2)-5R (NCK1 Human)
    Tags
    His6 and MBP
    Expression
    Bacterial
    Mutation
    construct contains only repeats of the second SH3…
    Promoter
    Lac
    Available Since
    Feb. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    112089-plasmid-map-sequence-id-236082

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  26. ACBE

    Plasmid
    #204607
    Purpose
    Mammalian expression of adenine-to-cytosine base editor ACBE
    Depositor
    Dali Li
    Article
    Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103
    Insert
    bpNLS-N terminal of nSpCas9-linker-mAAG (R165E/Y179F)-TadA-8e-linker-C terminal of nSpCas9-bpNLS-P2A-GFP
    Use
    CRISPR
    Tags
    6*His
    Expression
    Mammalian
    Mutation
    mAAG (R165E/Y179F); S. pyogenes Cas9 (D10A)
    Available Since
    Aug. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    204607-plasmid-map-sequence-id-404719

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  32. PLC-TIR-GFP

    Plasmid
    #52736
    Purpose
    Retroviral vector expresses the lipid binding domain of PLCd1 fused to the TIR of hTIRAP and GFP. Also contains IRES-hCD2 as selectable marker
    Depositor
    Jonathan Kagan
    Article
    Bonham et al Cell. 2014 Feb 13;156(4):705-16. doi: 10
    Insert
    Toll/IL-1 Receptor adaptor protein (TIRAP Human)
    Use
    Retroviral
    Tags
    GFP and PLCd1 Lipid binding
    Expression
    Mammalian
    Mutation
    Deleted amino acids 1-85
    Available Since
    June 18, 2014
    Availability
    Academic Institutions and Nonprofits only

  39. pG2J

    Plasmid
    #102882
    Purpose
    Expresses OsTIR1 via the ADH1 promoter, and the mGFP-AID via the TEF1 promoter in yeast. Both cassettes are integrated in HO locus. Assembly and characterization of the auxin-inducible degradation.
    Depositor
    Matthias Heinemann
    Article
    Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi
    Inserts
    OsTIR1
    mGFP-AID
    Tags
    AID(71-114)
    Expression
    Yeast
    Promoter
    ADH1 and TEF1
    Available Since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    102882-plasmid-map-sequence-id-199289

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Showing: 9841 - 9880 of 10326 results