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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 9921 - 9960 of 23042 results

  2. pcDNA3.1-TRPV1exCellHalo

    Plasmid
    #220307
    Purpose
    Mammalian expression vector for a subunit of rat TRPV1 with circularly permutated HaloTag inserted between the S5 and S6 transmembrane helices
    Depositor
    Eric Senning
    Article
    Mott et al J Gen Physiol. 2024 Oct 7;156(10):e20231
    Insert
    rTRPV1exCellHalo (Trpv1 Rat)
    Tags
    circularly permutated Halotag (Deo et al., Nat Ch…
    Expression
    Mammalian
    Mutation
    linker included to insert cpHaloTag (
    Promoter
    CMV promoter
    Available Since
    July 30, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    220307-plasmid-map-sequence-id-436649

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  6. pSCM001

    Plasmid
    #124605
    Purpose
    Expresses mCherry-pHluorin fusion protein in E. coli, a fluorescent reporter for cytoplasmic pH
    Depositor
    David Summers
    Article
    Zarkan et al Sci Rep. 2019 Mar 7;9(1):3868. doi: 10.1
    Insert
    mCherry-pHluorin
    Tags
    mCherry-(Gly-Gly-Ser)x2-pHluorin translational fu…
    Expression
    Bacterial
    Promoter
    pDAB
    Available Since
    May 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    124605-plasmid-map-sequence-id-242761

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  13. p46Cpf1-OP2

    Plasmid
    #98592
    Purpose
    Produces lambda Red and Cpf1 for recombination and selection. The plasmid is combined with a donor plasmid (with crRNA and template) for rapid genome editing in E.coli.
    Depositor
    Qiong Wu
    Article
    Ao et al Front Microbiol. 2018 Oct 9;9:2307. doi:
    Insert
    FnCpf1
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    Codon optimized for E. coli
    Promoter
    araBAD
    Available Since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    98592-plasmid-map-sequence-id-201329

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  14. pVenusNfN

    Plasmid
    #128564
    Purpose
    Protein interaction screening tool with Gateway-compatible BiFC vectors comprising of a set of 4 vectors permiting N or C fusions of candidate genes to the N or C fragment of Split-Venus.
    Depositor
    Oliver Vugrek
    Article
    Lepur et al J Biomol Screen. 2016 Dec;21(10):1100-11
    Insert
    Venus
    Expression
    Mammalian
    Available Since
    Jan. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    128564-plasmid-map-sequence-id-321698

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  15. NSSCP

    Plasmid
    #183190
    Purpose
    mRFP-PopZ expressed under J23116 promoter; split N terminal of TEV protease-SpmXΔC expressed under Tac promoter; SpmXΔC-split C terminal of TEV protease expressed under Tac promoter; DivIVA-sfGFP-TEV
    Depositor
    Hsiao-Chun Huang
    Article
    Hong et al ACS Synth Biol. 2021 Aug 20;10(8):1830-1
    Inserts
    mRFP-PopZ
    DivIVA-GFP(tevR-50)
    SpmX-TEV(C)
    TEV(N)-SpmX
    Expression
    Bacterial
    Available Since
    July 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    183190-plasmid-map-sequence-id-366594

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  16. pVenusCfC

    Plasmid
    #128567
    Purpose
    Protein interaction screening tool with Gateway-compatible BiFC vectors comprising of a set of 4 vectors permiting N or C fusions of candidate genes to the N or C fragment of Split-Venus.
    Depositor
    Oliver Vugrek
    Article
    Lepur et al J Biomol Screen. 2016 Dec;21(10):1100-11
    Insert
    Venus
    Expression
    Mammalian
    Available Since
    Jan. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    128567-plasmid-map-sequence-id-321691

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  17. pVenusCfN

    Plasmid
    #128566
    Purpose
    Protein interaction screening tool with Gateway-compatible BiFC vectors comprising of a set of 4 vectors permiting N or C fusions of candidate genes to the N or C fragment of Split-Venus.
    Depositor
    Oliver Vugrek
    Article
    Lepur et al J Biomol Screen. 2016 Dec;21(10):1100-11
    Insert
    Venus
    Expression
    Mammalian
    Available Since
    Jan. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    128566-plasmid-map-sequence-id-321694

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  24. ZJOM82

    Plasmid
    #133648
    Purpose
    Integration of NAB2-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast nucleus.
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-NAB2-mCherry (NAB2 Budding Yeast)
    Tags
    mCherry
    Expression
    Yeast
    Available Since
    Jan. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    133648-plasmid-map-sequence-id-269551

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  30. ZJOM64

    Plasmid
    #133655
    Purpose
    Integration of COX4-DuDre as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast mitochondria.
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-COX4-DuDre (COX4 Budding Yeast)
    Tags
    DuDre
    Expression
    Yeast
    Available Since
    April 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    133655-plasmid-map-sequence-id-292149

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  34. pDR111_mannose_Bgal NLS

    Plasmid
    #188398
    Purpose
    Expresses beta-galactosidase by D-mannose promoter for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.
    Depositor
    Christopher Contag
    Article
    Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:
    Insert
    lacZ
    Tags
    PhoD secretion peptide and SV40 NLS
    Expression
    Bacterial
    Promoter
    D-mannose
    Available Since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    188398-plasmid-map-sequence-id-370473

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Showing: 9921 - 9960 of 23042 results