We narrowed to 989 results for: Streptococcus

1. pT3TS-Hypa-nCas9n-A69

   Plasmid

   #125645

   Purpose

   micro-injection of poly-adenylated Hypa-nCas9n RNA

   Depositor

   Jason Berman

   Article

   Prykhozhij et al Methods Mol Biol. 2020;2115:385-405. doi

   Insert

   Hypa-nCas9n
   

   Use

   Rna in vitro transcription vector

   Mutation

   N692A, M694A, Q695A, H698A from wild-type Cas9

   Promoter

   T3

   Available Since

   July 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCF704

   Plasmid

   #121658

   Purpose

   U6-sgRNA EFS-ProCas9-Flavi-P2A-Puro

   Depositor

   David Savage

   Article

   Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

   Insert

   Cas9(C)
   

   Use

   Lentiviral

   Promoter

   U6

   Available Since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCF712

   Plasmid

   #121663

   Purpose

   U6-sgRNA EF1a-ProCas9-Flavi-P2A-Puro

   Depositor

   David Savage

   Article

   Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

   Insert

   Cas9(C)
   

   Use

   Lentiviral

   Promoter

   U6

   Available Since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSBtet-Hyg-dCas9-KRAB-MeCP2_hU6-SapI

   Plasmid

   #196082

   Purpose

   (Empty Backbone) Inducible CRISPRi vector conferring hygromycin B resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.

   Depositor

   Joanna Bereta

   Article

   Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

   Inserts

   dCas9-KRAB-MeCP2 repressor
   hU6 promoter and gRNA scaffold
   Aminoglycoside phosphotransferase
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Catalytically dead mutant of the Cas9 endonucleas…

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSBtet-Bla-dCas9-KRAB-MeCP2_hU6-SapI

   Plasmid

   #196080

   Purpose

   (Empty Backbone) Inducible CRISPRi vector conferring blasticidin S resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.

   Depositor

   Joanna Bereta

   Article

   Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

   Inserts

   dCas9-KRAB-MeCP2 repressor
   hU6 promoter and gRNA scaffold
   Blasticidin S deaminase
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Catalytically dead mutant of the Cas9 endonucleas…

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOSIP-KH-RBS2-dCas9

   Plasmid

   #182740

   Purpose

   Integrative plasmid at the HK022 site in E. coli carrying dCas9 under the control of a Ptet promoter

   Depositor

   David Bikard

   Article

   Rousset et al PLoS Genet. 2018 Nov 7;14(11):e1007749.

   Insert

   dCas9
   

   Use

   CRISPR; Integrative vector

   Promoter

   Ptet

   Available Since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRCT

   Plasmid

   #60621

   Purpose

   Plasmid encoding iCas9, tracrRNA and crRNAs

   Depositor

   Huimin Zhao

   Article

   Bao et al ACS Synth Biol. 2014 Sep 19.

   Inserts

   iCas9
   tracrRNA
   

   Use

   CRISPR

   Tags

   FLAG and SV40 NLS

   Expression

   Yeast

   Mutation

   changed Aspartate 147 to Tyrosine, Proline 411 to…

   Promoter

   RPR1p and TEF1p

   Available Since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAc-sgRNA-Cas9

   Plasmid

   #49330

   Purpose

   Expresses sgRNA and Cas9-Puro in Drosophila S2 cells

   Depositor

   Ji-Long Liu

   Article

   Bassett et al Biol Open. 2013 Dec 10. pii: bio.2013712

   Inserts

   Cas9
   dU6-sgRNA
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Insect

   Mutation

   Human codon optimised

   Promoter

   Actin-5c and Drosophila U6

   Available Since

   Dec. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEasyG1_nat

   Plasmid

   #184911

   Purpose

   Template to generate via PCR a single gRNA for expression in S. cerevisiae

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Expression

   Bacterial and Yeast

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-Flag-hGSDME

    Plasmid

    #218939

    Purpose

    Expression of N-terminal FLAG tagged human gasdermin E

    Depositor

    Christopher LaRock

    Article

    LaRock et al Nature. 2022 May;605(7910):527-531. doi:

    Insert

    human GSDME (GSDME Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. All_in_one_CRISPR/Cas9_LacZ

    Plasmid

    #74293

    Purpose

    "All-in-one" CRISPR/Cas9 (wt) plasmid for cloning of custom gRNA with blue/white screening

    Depositor

    Lynne Postovit

    Article

    Findlay et al PLoS One. 2016 Apr 18;11(4):e0153901. do

    Inserts

    LacZ-alpha
    Cas9
    mCherry
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Promoter

    Lac promoter, SV40, and T7 promoter

    Available Since

    May 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC19_CRISPR_DpmrA

    Plasmid

    #160903

    Purpose

    CRISPR_Cas9, lambda red recombineering

    Depositor

    Anne-Catrin Uhlemann

    Article

    McConville et al Cell Rep. 2020 Oct 27;33(4):108313. doi:

    Inserts

    Cas9
    Lambda Red Recombineering Genes
    Zeocin Resistance Cassette
    

    Promoter

    araBAD

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMSCV-Cas9-2A-GFP-sgRNA

    Plasmid

    #124889

    Purpose

    Contains spCas9-2A-GFP and guide RNA scaffold in single retroviral vector

    Depositor

    Harvey Lodish

    Article

    Li et al Haematologica. 2016 Jun;101(6):e216-9. d

    Insert

    Cas9
    

    Use

    Retroviral

    Tags

    2A-GFP

    Promoter

    MSCV

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. gRNA NIA TLS2

    Plasmid

    #196981

    Purpose

    Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Each gRNA is fused to TLS2 mobility signal that confers graft mobility.

    Depositor

    Friedrich Kragler

    Article

    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    AtNIA1 gRNAs fused to TLS2 tags
    

    Expression

    Plant

    Promoter

    pU6-26, pU6-29

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHis-parallel1-NLSH2BCas9

    Plasmid

    #112065

    Purpose

    Expression and purification of NLSH2BCas9 for fungal gene editing

    Depositor

    Jeff Coleman

    Article

    Wang et al Fungal Genet Biol. 2018 May 12. pii: S10

    Insert

    NLS of the Fusarium oxysporum histone H2B fused to SpCas9 that was codon optimized fro Aspergillus niger
    

    Use

    CRISPR

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Cas9 was codon optimized for Aspergillus niger

    Promoter

    promoter in vector

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. HiFi dCas9 VPR

    Plasmid

    #188510

    Purpose

    Expresses FLAG tagged HiFi dCas9 VPR

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A/R691A/H840A

    Promoter

    EF1a

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. Pb-TRE3G-SpCas9-IRES-blast

    Plasmid

    #195506

    Purpose

    Inducible Piggybac vector expressing SpCas9 with Blasticidin selection

    Depositor

    Joanna Wysocka

    Article

    Bajpai et al Science. 2023 Aug 11;381(6658):eade6289.

    Insert

    spCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. dCas9-10xSunTag-P2A-tagBFP

    Plasmid

    #174141

    Purpose

    Expresses the dCas9-10xSunTag-tagBFP fusion protein for the recruitment of scFv(GCN4)-fused effector domains. A shorter version of the Addgene 60903 plasmid.

    Depositor

    Albert Jeltsch

    Article

    Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

    Insert

    dCas9, 10xSunTag, tagBFP
    

    Tags

    HA and tagBFP

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. Puro-Cas9 donor

    Plasmid

    #58409

    Purpose

    Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus

    Depositor

    Danwei Huangfu

    Article

    Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3xFlag, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)

    Plasmid

    #78231

    Purpose

    Expresses Cas9 protein, under the control of the TEF1 promoter, and CYC terminator. KanMX resistance

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    Cas9
    

    Expression

    Yeast

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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