167,948 results

1. dGFP-Rab3a

   Plasmid

   #170864

   Purpose

   Mammalian expression of dGFP-Rab3a.

   Depositor

   Justin Taraska

   Article

   Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

   Insert

   Rab-3a (Rab3a Mouse)
   

   Tags

   dEGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV.Syn.GCaMP6m.WPRE.SV40 (AAV5)

   Viral Prep

   #100841-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pAAV.Syn.GCaMP6m.WPRE.SV40 (#100841). In addition to the viral particles, you will also receive purified pAAV.Syn.GCaMP6m.WPRE.SV40 plasmid DNA. Syn-driven GCaMP6m calcium sensor. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Douglas Kim, GENIE Project

   Article

   Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

   Promoter

   Syn

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

3. pENTR-GFP-MDC1 (770-7)

   Plasmid

   #26284

   Depositor

   Eric Campeau

   Insert

   Mediator of DNA damage checkpoint 1 (MDC1 Human)
   

   Use

   Entry vector

   Tags

   GFP

   Available Since

   Oct. 18, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. CRISPi Kit

   Plasmid Kit

   #1000000136

   Purpose

   CRISPi Kit contains elements for CRISPR/Cas9 mediated genome editing in yeast P. pastoris. Contains 7 different backbone (BB3) vectors with Cas9/ sgRNA expression cassettes with NTC, hphMX, or kanMX.

   Depositor

   Brigitte Gasser

   Available Since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

5. pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (AAV PHP.eB)

   Viral Prep

   #104492-PHPeB

   Purpose

   Ready-to-use AAV PHP.eB particles produced from pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (#104492). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP7f-WPRE plasmid DNA. Syn-driven, Cre-dependent GCaMP7f calcium sensor. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Promoter

   Syn

   Available Since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

6. lenti-SAM-hygro

   Plasmid

   #138955

   Purpose

   Lentiviral vector for co-expressing spCas9, dRNA-MS2, p65 and HSF1 fused to MS2 binding protein, and hygromycin resistance gene

   Depositor

   Nadya Dimitrova

   Article

   Olivero et al Mol Cell. 2020 Feb 20;77(4):761-774.e8.

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6 AND EF1a

   Available Since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. MAC_C_INSR

   Plasmid

   #187772

   Purpose

   MAC-tagged gene expression of human INSR

   Depositor

   Markku Varjosalo

   Article

   Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

   Insert

   INSR (INSR Human)
   

   Expression

   Mammalian

   Available Since

   Aug. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPB-CAG-hCas3

   Plasmid

   #134920

   Purpose

   Components for genome editing in mammalian cells with pCAG-All-in-one-hCascade and pBS-U6-crRNA-targeted.

   Depositor

   Tomoji Mashimo

   Article

   Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

   Insert

   Cas3 with bpNLS
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHes1(2.5k)-luc

   Plasmid

   #43806

   Depositor

   Ryoichiro Kageyama

   Article

   Takebayashi et al J Biol Chem. 1994 Feb 18;269(7):5150-6.

   Insert

   Hes1 Promoter (Hes1 Mouse)
   

   Use

   Luciferase

   Tags

   Firefly luciferase

   Expression

   Mammalian

   Mutation

   Contains the murine Hes1 promoter

   Promoter

   Hes1 Promoter

   Available Since

   March 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. PX552

    Plasmid

    #60958

    Purpose

    pAAV-U6sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). AAV plasmid for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei.

    Depositor

    Feng Zhang

    Article

    Swiech et al Nat Biotechnol. 2014 Oct 19. doi: 10.103

    Inserts

    U6_(SpaI)_sgRNA
    Syn_EGFP-KASH
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Expression

    Mammalian

    Promoter

    Synapsin and U6

    Available Since

    Nov. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCI-T7Max-UTR1-deGFP-8xHis-T500

    Plasmid

    #178422

    Purpose

    Expression of deGFP via a T7 Promoter in TXTL

    Depositor

    Kate Adamala

    Article

    Deich et al J Biol Eng. 2023 Jan 23;17(1):4. doi: 10

    Insert

    deGFP
    

    Use

    Synthetic Biology

    Tags

    8xHis Tag

    Expression

    Bacterial

    Promoter

    T7Max

    Available Since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSB1C3-J23110-B0034-mRFP1_Yellow

    Plasmid

    #160443

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Yellow chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

    Insert

    promoter, RBS, mRFP1E_Yellow
    

    Use

    Synthetic Biology

    Mutation

    BioBrick sites removed

    Available Since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVX-Tight-Puro_Nkx6.2

    Plasmid

    #64846

    Purpose

    A lentiviral vector for the expression of mouse Nkx6.2 under doxycyline control

    Depositor

    Paul Tesar

    Article

    Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

    Insert

    Nkx6.2 (Nkx6-2 Mouse)
    

    Use

    Lentiviral

    Expression

    Bacterial and Mammalian

    Available Since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNCS Citrine

    Plasmid

    #91761

    Purpose

    Bacterial expression of a fluorescent protein, Citrine

    Depositor

    Roger Tsien, Erik Rodriguez

    Article

    Griesbeck et al J Biol Chem. 2001 Aug 3;276(31):29188-94

    Insert

    Citrine
    

    Tags

    Hexa-Histidine tag, Xpress epitope for detection …

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCI-EGFP-NR2a wt

    Plasmid

    #45445

    Depositor

    Andres Barria, Robert Malinow

    Article

    Barria et al Neuron. 2002 Jul 18;35(2):345-53.

    Insert

    NR2A (Grin2a Rat)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    EGFP inserted after the predicted signal peptide …

    Promoter

    CMV

    Available Since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. SpCas9-CBE6b-V106W

    Plasmid

    #215825

    Purpose

    Express CBE6b V106W (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

    Insert

    CBE6b V106W-SpCas9-2xUGI (cas9 )
    

    Use

    In vitro transcription template

    Available Since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. TFORF3358

    Plasmid

    #144834

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    STAT3 (STAT3 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEF1a-BirA-V5-neo

    Plasmid

    #100548

    Purpose

    Expresses C-terminal V5-tagged BirA biotin ligase

    Depositor

    Jian Xu

    Article

    Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.

    Insert

    C-terminal V5-tagged BirA
    

    Tags

    V5, His

    Expression

    Mammalian

    Promoter

    Human EF1a

    Available Since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. mCherry-cGAS-HA

    Plasmid

    #231895

    Purpose

    Expression of cyclic GMP/AMP synthase (cGAS), as a nuclear rupture marker

    Depositor

    Lukas Kapitein

    Article

    Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

    Insert

    cGAS (CGAS Human)
    

    Tags

    3xHA and mCherry

    Expression

    Mammalian

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHCMV_COCVmut_G

    Plasmid

    #207262

    Purpose

    Mammalian expression of a mutant Cocal virus G protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    COCVmut_G
    

    Expression

    Mammalian

    Mutation

    K47A/Y209A/R354A; mammalian codon optimization

    Promoter

    CMV promoter

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. pD649-HAsp-EDA-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156768

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    EDA (EDA Human)
    

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. pLv-CMV-MyoD-ER(T)

    Plasmid

    #26809

    Depositor

    Jeffrey Chamberlain

    Article

    Kimura et al Hum Mol Genet. 2008 Aug 15. 17(16):2507-

    Insert

    MyoD-ER(T) (Myod1 Mouse)
    

    Use

    Lentiviral

    Available Since

    Sept. 12, 2011

    Availability

    Academic Institutions and Nonprofits only

23. pAAV-CaMKII-PinkyCaMP

    Plasmid

    #232858

    Purpose

    An AAV vector expressing the mScarlet based calcium indicator PinkyCaMP under CaMKII promoter

    Depositor

    Olivia Masseck

    Article

    Fink et al bioRxiv [Preprint]. 2025 Jan 11:2024.12.

    Insert

    PinkyCaMP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CaMKII

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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24. Arf4-GFP

    Plasmid

    #39556

    Depositor

    Paul Melancon

    Article

    Chun et al Mol Biol Cell. 2008 Aug;19(8):3488-500.

    Insert

    ADP ribosylaion factor 4 (ARF4 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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25. pJeM1

    Plasmid

    #135088

    Purpose

    rhamnose-inducible expression vector for replication in E. coli and P. putida

    Depositor

    Josef Altenbuchner

    Article

    Jeske et al Appl Microbiol Biotechnol. 2010 Feb;85(6

    Insert

    rhaR rhaS rhaPBAD eGFP, mob (rhaR E.coli)
    

    Expression

    Bacterial

    Available Since

    Feb. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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26. SPYtag-Histag-Avidin

    Plasmid

    #214836

    Purpose

    This plasmid encodes a protein (SPYtag-Histag-Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

    Depositor

    Hironori Funabiki

    Article

    Arimura et al eLife. 2025 May 20;13:RP103486. doi: 10.

    Insert

    SPYtag-Histag-Avidin
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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27. pCMV6-G1-ATP8-Myc-FLAG (G418)

    Plasmid

    #86850

    Purpose

    mammalian expression of ATP8 with mitochondrial targeting sequence, Myc, and FLAG tags

    Depositor

    Matthew O'Connor

    Article

    Boominathan et al Nucleic Acids Res. 2016 Nov 2;44(19):934

    Insert

    ATP8 (ATP8 Human)
    

    Tags

    ATP5G1 (G1) (MQTAGALFISPALIRCCTRGLIRPVSASFLNS PVN…

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    CMV

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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28. pgLAP4

    Plasmid

    #19705

    Depositor

    Peter Jackson

    Insert

    pgLAP4
    

    Tags

    Flag, S peptide, and Tev

    Expression

    Mammalian

    Available Since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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29. pcDNA3.1-HDAC2-H141A

    Plasmid

    #115346

    Purpose

    Expresses mouse HDAC2 cDNA with H141A point mutation

    Depositor

    Javier Gonzalez-Maeso

    Article

    Kurita et al Nat Neurosci. 2012 Sep;15(9):1245-54. do

    Insert

    Histone deacetylase 2 (Hdac2 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    H141A

    Promoter

    CMV

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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30. CMVTO-SEAP-26Sfur-3TM-tevs-AAMP

    Plasmid

    #179633

    Purpose

    SEAP RELEASE construct with AAMP motif control

    Depositor

    Xiaojing Gao

    Article

    Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

    Insert

    SEAP RELEASE
    

    Expression

    Mammalian

    Available Since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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