We narrowed to 17,432 results for: She

1. pYLsgRNA-OsU6a

   Plasmid

   #66194

   Purpose

   cloning of sgRNAs for expression in plants

   Depositor

   Yao-Guang Liu

   Article

   Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

   Type

   Empty backbone

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   OsU6a

   Available since

   Sept. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pv6_TAF3_2xPhd

   Plasmid

   #179399

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of TAF3_2xPhd

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   TAF3_2xPhd
   

   Use

   Tags

   AviTag and EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAGGS

   Available since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET26b_AT118-L-V5-His

   Plasmid

   #222848

   Purpose

   E. coli periplasmid expression plasmid encoding Nb. AT118-L, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) with reduced polyreactivity

   Depositor

   Andrew Kruse

   Article

   Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

   Insert

   AT118-L
   

   Use

   Tags

   V5-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pv6_CBX7_2xChromo

   Plasmid

   #179396

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_2xChromo

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   CBX7_2xChromo
   

   Use

   Tags

   AviTag and EGFP

   Expression

   Mammalian

   Mutation

   G113V in flexible linker

   Promoter

   CAGGS

   Available since

   Sept. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDL023_Tub-rb_IgG_HC

   Plasmid

   #193620

   Purpose

   α-Tubulin heavy chain variable region + rabbit heavy chain constant regions + exogenous N-term signal peptide. For co-expression with pDL024 in HEK293 cells.

   Depositor

   Jennifer DeLuca

   Article

   DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

   Insert

   Anti-Tubulin scFv heavy variable + rabbit constant chain
   

   Use

   Tags

   signal peptide

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 21, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pDL024_Tub-rb_IgG_LC

   Plasmid

   #193621

   Purpose

   α-Tubulin light chain variable region + rabbit light chain constant region + exogenous N-term signal peptide. For co-expression with pDL023 in HEK293 cells.

   Depositor

   Jennifer DeLuca

   Article

   DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

   Insert

   Anti-Tubulin scFv light variable + rabbit constant chain
   

   Use

   Tags

   signal peptide

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 21, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pv6_MBD1_MBD

   Plasmid

   #179404

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1_MBD

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   MBD1_MBD
   

   Use

   Tags

   AviTag and EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAGGS

   Available since

   Aug. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pv6_CBX7_Chromo_TAF3_Phd

   Plasmid

   #179400

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_Chromo - TAF3_Phd

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   CBX7_Chromo - TAF3_Phd
   

   Use

   Tags

   AviTag and EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAGGS

   Available since

   Sept. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Tol2-elavl3-GCaMP6s

   Plasmid

   #59531

   Purpose

   Expressed GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backbone

   Depositor

   Misha Ahrens

   Article

   Vladimirov et al Nat Methods. 2014 Jul 27. doi: 10.1038/n

   Inserts

   elavl3 promoter (elavl3 Zebrafish)
   GCaMP6s
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 12, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLX301

    Plasmid

    #25895

    Depositor

    David Root

    Article

    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTrc99S-ssDsbA-CRM197-4xDQNAT

    Plasmid

    #128403

    Purpose

    Periplasmic expression of CRM197 with C terminal 4xDQNAT glycosylation sites in E. coli

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    Diphtheria toxin
    

    Use

    Tags

    4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

    Expression

    Bacterial

    Mutation

    Inactivating G52E mutation

    Promoter

    trc

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFUGW-FerH-ffLuc2-eGFP

    Plasmid

    #71393

    Purpose

    Lentiviral vector of luciferase-eGFP fusion gene driven by FerH promoter

    Depositor

    Glenn Merlino

    Article

    Day et al Pigment Cell Melanoma Res. 2009 Jun;22(3

    Insert

    FerH-ffLuc2-eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    FerH

    Available since

    Dec. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLenti6/V5/DEST-VPS35

    Plasmid

    #21691

    Depositor

    Lynda Chin

    Article

    Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

    Insert

    Vacuolar Protein Sorting 35 homolog (VPS35 Human)
    

    Use

    Lentiviral; Invitrogen gateway destination vector

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLenti-sgRNA(lib)-puro

    Plasmid

    #119976

    Purpose

    Lenti sgRNA cloning backbone with CMV-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Wensheng Wei

    Article

    Liu et al Nat Biotechnol. 2018 Nov 5. pii: nbt.428

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pACTA2_HL-P2A-eGFP-PGK-PuroR-ACTA2_HR

    Plasmid

    #126705

    Purpose

    donor vector for targeting a 2A peptide followed by green fluorescent reporter to the human ACTA2 locus at the end of the endogenous coding sequence

    Depositor

    Darrell Kotton

    Article

    Kwong et al Stem Cell Reports. 2019 Sep 10;13(3):499

    Insert

    GFP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p5E-mmCdh5pro (JDW 1239)

    Plasmid

    #224478

    Purpose

    Gateway compatible 5' entry clone containing the Murine Cdh5 promoter

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Cdh5 promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCA12-pHR-SFFV-BFP-B23

    Plasmid

    #199443

    Purpose

    Expression of BFP-B23 in mammalian cells

    Depositor

    Baohui Chen

    Article

    Fu et al J Cell Biol. 2023 Jan 2;222(1):e20220211

    Insert

    B23 (NPM1 Human)
    

    Use

    Lentiviral

    Tags

    TagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLX302

    Plasmid

    #25896

    Depositor

    David Root

    Article

    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-ABEmax-NRTH

    Plasmid

    #136922

    Purpose

    Expresses ABEmax-NRTH in mammalian cells

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    ABEmax-NRTH
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    see manuscript

    Promoter

    CMV

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-ABEmax-NRCH

    Plasmid

    #136923

    Purpose

    Expresses ABEmax-NRCH in mammalian cells

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    ABEmax-NRCH
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    see manuscript

    Promoter

    CMV

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. IKK-2 K44M

    Plasmid

    #11104

    Depositor

    Anjana Rao

    Article

    Mercurio et al Science. 1997 Oct 31. 278(5339):860-6.

    Insert

    IKK-2 (IKBKB Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    K44M, kinase inactive

    Promoter

    Available since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCGN-ATF6 (1-373)

    Plasmid

    #27173

    Purpose

    For mammalian expression of the cytoplasmic domain of human ATF6with an HA tag.

    Depositor

    Ron Prywes

    Article

    Wang et al J Biol Chem. 2000 Sep 1. 275(35):27013-2

    Insert

    ATF6 (ATF6 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Contains only aa 1-373 (please see depositor comm…

    Promoter

    Available since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. (401) pAAV CMV-ZsGreen U6-gRNA

    Plasmid

    #163021

    Purpose

    Fluorescent reporter for Sa gRNA (Bsa1 sites)

    Depositor

    Tonia Rex

    Article

    Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

    Insert

    Zs Green
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Stargazin-mCherry-FRB

    Plasmid

    #172443

    Purpose

    Expression of plasma membrane protein Stargazin tagged with mCherry-FRB

    Depositor

    Stephen Royle

    Article

    Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    Cacng2 (Cacng2 Mouse)
    

    Use

    Tags

    mCherry-HA-FRB

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGMC00028 (aka pTS0057)

    Plasmid

    #200832

    Purpose

    AAVS1 guide RNA cloned into the lentiSAM puro backbone

    Depositor

    Raj Chari

    Article

    Yang et al Sci Adv. 2024 Feb 16;10(7):eadj2445. doi

    Insert

    AAVS1 guide RNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pYLsgRNA-AtU6-29

    Plasmid

    #66203

    Purpose

    cloning of sgRNAs for expression in plants

    Depositor

    Yao-Guang Liu

    Article

    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

    Type

    Empty backbone

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. AICSDP-29: LMNB1-mTagRFP-T

    Plasmid

    #114403

    Purpose

    Homology arms and mTagRFPT-linker sequence for N-terminus tagging of human LMNB1

    Depositor

    Allen Institute for Cell Science

    Insert

    LMNB1 Homology Arms with mTagRFPT-linker (LMNB1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    mTagRFPT-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    Sept. 4, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. IRES-GFP FoxG1

    Plasmid

    #14935

    Depositor

    Joan Massague

    Article

    Seoane et al Cell. 2004 Apr 16. 117(2):211-23.

    Insert

    FoxG1 (FOXG1 Human)
    

    Use

    Tags

    IRES-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 17, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. T7-VEE-mCherry

    Plasmid

    #193355

    Purpose

    Self-replicating RNA vector expressing mCherry fluorescent protein + PuroR

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    mCherry
    

    Use

    Venezuelan equine encephalitis (vee) virus rna re…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3-Flag-Tet1 CD

    Plasmid

    #70129

    Purpose

    Express flag-tagged mouse Tet1 catalytic domain.

    Depositor

    Yi Zhang

    Article

    Ito et al Science. 2011 Sep 2;333(6047):1300-3. do

    Insert

    Ten-Eleven Translocation 1 (Tet1 Mouse)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    The plasmid expresses TET1 catalytic domain.

    Promoter

    Available since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAAV-Ef1a-DIO eNpHR 3.0-EYFP

    Plasmid

    #26966

    Purpose

    AAV expression of eNpHR 3.0 fused to EYFP driven by EF1a promoter for optogenetic inhibition

    Depositor

    Karl Deisseroth

    Article

    Gradinaru et al Cell. 2010 Apr 2. 141(1):154-65.

    Has Service

    AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    eNpHR 3.0
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    ER export at 3' end of gene. Trafficking sig…

    Promoter

    EF1alpha

    Available since

    Jan. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCI-Neo-PTPRT (WT)

    Plasmid

    #16630

    Depositor

    Bert Vogelstein

    Article

    Wang et al Science. 2004 May 21. 304(5674):1164-6.

    Insert

    PTPRT (PTPRT Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

33. pMXs-cMyc

    Plasmid

    #233183

    Purpose

    Retroviral expression of c-Myc

    Depositor

    Kate Galloway

    Article

    Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

    Insert

    c-Myc (Myc Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pUC19-eGFP-P2A-dTAG-Ing3

    Plasmid

    #170754

    Purpose

    Donor plasmid for eGFP-P2A-2xHA-dTAG knock-in into the N-terminus of the mouse Ing3 locus

    Depositor

    Kristian Helin

    Article

    Damhofer et al STAR Protoc. 2021 Jun 2;2(2):100575. doi

    Insert

    eGFP-P2A-2xHA-dTAG
    

    Use

    Mouse Targeting

    Tags

    eGFP-P2A-2xHA-dTAG

    Expression

    Mutation

    Phenylalanine 36 to valine in FKBP12

    Promoter

    none

    Available since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA3-6XHis-human SUMO 1

    Plasmid

    #133770

    Purpose

    express 6Xhis- SUMO 1 protein

    Depositor

    Martine Roussel

    Article

    Tago et al Proc Natl Acad Sci U S A. 2005 May 24. 1

    Insert

    SUMO 1 (SUMO1 Human)
    

    Use

    Tags

    6XHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pGL4.10/RSV_SF3B3-miniXon_Luciferase

    Plasmid

    #174660

    Purpose

    Plasmid containing the SF3B3-miniXon/Firefly luciferase cassette. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-miniXon by LMI070.

    Depositor

    Beverly L. Davidson

    Article

    Monteys et al Nature. 2021 Jul 28. pii: 10.1038/s41586

    Insert

    miniXon cassette
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Kozak ATG initiation sequence in middle exon

    Promoter

    RSV

    Available since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pYLsgRNA-AtU6-1

    Plasmid

    #66202

    Purpose

    cloning of sgRNAs for expression in plants

    Depositor

    Yao-Guang Liu

    Article

    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

    Type

    Empty backbone

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pSP64-hKCNE1

    Plasmid

    #53049

    Purpose

    Expresses beta subunit for IKs channel in Xenopus oocytes

    Depositor

    Michael Sanguinetti

    Article

    Sanguinetti et al Nature. 1996 Nov 7;384(6604):80-3.

    Insert

    Potassium voltage-gated channel subfamily E member 1 (KCNE1 Human)
    

    Use

    Xenopus oocyte expression

    Tags

    Expression

    Mutation

    Promoter

    SP6

    Available since

    May 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pYLsgRNA-AtU3d/LacZ

    Plasmid

    #66201

    Purpose

    cloning of sgRNAs for expression in plants

    Depositor

    Yao-Guang Liu

    Article

    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

    Type

    Empty backbone

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    AtU3d

    Available since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCMV5-Flag-HDAC3

    Plasmid

    #63676

    Purpose

    Mammalian expression of Flag tagged HDAC3

    Depositor

    Qingbo Xu, Lingfang Zeng

    Article

    Zeng et al J Cell Biol. 2006 Sep 25;174(7):1059-69.

    Insert

    HDAC3 (Hdac3 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only