We narrowed to 13,844 results for: cas9 genes

1. Ssh1 gRNA#2

   Plasmid

   #163395

   Purpose

   Cas9-mediated knockout of Ssh1 in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   ssh1 (Ssh1 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Ssh1 gRNA#1

   Plasmid

   #163394

   Purpose

   Cas9-mediated knockout of Ssh1 in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   ssh1 (Ssh1 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Ssh1 gRNA#3

   Plasmid

   #163396

   Purpose

   Cas9-mediated knockout of Ssh1 in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   ssh1 (Ssh1 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pmKaxxte

   Plasmid

   #113630

   Purpose

   to test Cas9 gRNA efficiency or HR-capacity

   Depositor

   Ken Mills

   Article

   Liberante et al Sci Rep. 2019 Feb 25;9(1):2678. doi: 10.

   Insert

   mKaxxte2.5
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. Fungal Toolkit for Modular Cloning (FTK)

   Plasmid Kit

   #1000000191

   Purpose

   Consists of 96 genetic parts as Modular Cloning-compatible entry vectors, including synthetic and native promoters, terminators, selection markers, and CRISPR components, for filamentous fungi.

   Depositor

   Arnold Driessen

   Available since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

6. Human Lentiviral sgRNA Library - Kinases

   Pooled Library

   #51044

   Purpose

   Sub-pool of Sabatini/Lander sgRNA library enriched for kinase targets.

   Depositor

   David Sabatini, Eric Lander

   Article

   Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

   Expression

   Mammalian

   Species

   Homo sapiens

   Use

   CRISPR and Lentiviral

   Available since

   Feb. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

7. Human Lentiviral sgRNA Library - Nuclear

   Pooled Library

   #51047

   Purpose

   Sub-pool of Sabatini/Lander sgRNA library enriched for nuclear targets.

   Depositor

   David Sabatini, Eric Lander

   Article

   Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

   Expression

   Mammalian

   Species

   Homo sapiens

   Use

   CRISPR and Lentiviral

   Available since

   Feb. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

8. Human Lentiviral sgRNA Library - Cell Cycle

   Pooled Library

   #51046

   Purpose

   Sub-pool of Sabatini/Lander sgRNA library enriched for cell cycle-related targets.

   Depositor

   David Sabatini, Eric Lander

   Article

   Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

   Expression

   Mammalian

   Species

   Homo sapiens

   Use

   CRISPR and Lentiviral

   Available since

   Feb. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

9. Human Lentiviral sgRNA Library - Control

   Pooled Library

   #51048

   Purpose

   Sub-pool of Sabatini/Lander sgRNA library enriched for control targets.

   Depositor

   David Sabatini, Eric Lander

   Article

   Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

   Expression

   Mammalian

   Species

   Homo sapiens

   Use

   CRISPR and Lentiviral

   Available since

   Feb. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pX459-dErbB4

    Plasmid

    #197357

    Purpose

    A knock-out vector for dog ErbB4.

    Depositor

    Michiyuki Matsuda

    Article

    Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

    Insert

    A gRNA targeting the dog ERBB4 gene and the cDNA of CRISPR-Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX459-dErbB1

    Plasmid

    #197354

    Purpose

    A knock-out vector for dog ErbB1.

    Depositor

    Michiyuki Matsuda

    Article

    Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

    Insert

    A gRNA targeting the dog EGFR gene and the cDNA of CRISPR-Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pX459-dErbB2

    Plasmid

    #197355

    Purpose

    A knock-out vector for dog ErbB2.

    Depositor

    Michiyuki Matsuda

    Article

    Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

    Insert

    A gRNA targeting the dog ERBB2 gene and the cDNA of CRISPR-Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX458-dErbB3

    Plasmid

    #197356

    Purpose

    A knock-out vector for dog ErbB3.

    Depositor

    Michiyuki Matsuda

    Article

    Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

    Insert

    A gRNA targeting the dog ERBB3 gene and the cDNA of CRISPR-Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. lentiCRISPR - CTRL sgRNA

    Plasmid

    #70662

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a non-targeting sgRNA element from U6 promoter. Lentiviral backbone

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    non-targeting sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. lentiCRISPR - AAVS1 sgRNA

    Plasmid

    #70661

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an AAVS1-targeting sgRNA element from U6 promoter. Lentiviral backbone

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    sgRNA against AAVS1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pARv-RFP

    Plasmid

    #60021

    Purpose

    pARv-RFP is a fluorescent reporter plasmid, that allows fast and easy evaluation of ZFN, TALEN or CRISPR/Cas9 activity in the transfected cells.

    Depositor

    Radislav Sedláček

    Article

    Kasparek et al FEBS Lett. 2014 Sep 18. pii: S0014-5793(

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJZ002

    Plasmid

    #120231

    Purpose

    CRISPR-cas9 targeting in E. coli with ampicillin resistance

    Depositor

    Bradley Moore

    Article

    Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. Brp-T2A-QF2w-Hsp70-3XP3-dsRed

    Plasmid

    #141094

    Purpose

    Template plasmid for inserting QF2w into the Brp gene of Aedes aegypti with CRISPR/Cas9, to generate a pan-neuronal driver line.

    Depositor

    Carolyn McBride

    Article

    Zhao et al Cell Rep Methods. 2021 Jul 26;1(3):10004

    Insert

    bruchpilot
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    coding sequence downstream of CRISPR cut site, sy…

    Promoter

    Available since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. Myog'-2A-mCherryNLS-PGK-Purodtk

    Plasmid

    #69547

    Purpose

    Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.

    Depositor

    Charles Gersbach

    Inserts

    2A-mCherry-NLS
    Myogenin 5' homology arm (Myog Mouse)
    Myogenin 3' homology arm (Myog Mouse)
    Puro-delta-tk
    

    Use

    Mouse Targeting

    Tags

    2x nuclear localization signal and PVT1-2A "…

    Expression

    Mutation

    Silent V205V mutation in myogenin gene to prevent…

    Promoter

    Available since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEPOR1CB0023

    Plasmid

    #117538

    Purpose

    Level1 Golden Gate Cassette: extended stem sgRNA cassette with AtU6-26 terminator for SpCas9, targeting NbPDS gene in N. benthamiana

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    sgRNA (Sp Cas9) - extended stem with U626 terminator
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCRISPR-PE-bacteria

    Plasmid

    #172715

    Purpose

    Expression of an E. coli codon optimized fusion protein of Cas9n-linker-M-MLV2 for prime editing

    Depositor

    Tilmann Weber

    Article

    Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

    Insert

    Cas9n-linker-M-MLV2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    H840A of Cas9

    Promoter

    Available since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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22. pMJ806

    Plasmid

    #39312

    Depositor

    Jennifer Doudna

    Article

    Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCW-SPIC

    Plasmid

    #162825

    Purpose

    Doxycycline-inducible overexpression of human SPIC

    Depositor

    Matthew Steinhauser

    Article

    Fazeli et al Mol Metab. 2020 Sep 28;42:101082. doi: 1

    Insert

    SPIC (SPIC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet ON

    Available since

    Feb. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMJ826

    Plasmid

    #39316

    Depositor

    Jennifer Doudna

    Article

    Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Histidine 840 to Alanine (H840A)

    Promoter

    T7

    Available since

    Aug. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMJ825

    Plasmid

    #39315

    Depositor

    Jennifer Doudna

    Article

    Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Aspartate 10 to Alanine (D10A)

    Promoter

    T7

    Available since

    Aug. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCMV-PEmax-SpG-P2A-hMLH1dn

    Plasmid

    #211550

    Purpose

    Prime editing in mammalian cells

    Depositor

    Yongsub Kim

    Article

    Jang et al Commun Biol. 2023 Jun 30;6(1):681. doi:

    Insert

    PEmax-SpG-P2A-hMLH1dn
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pTJV1Sc3-rpoB

    Plasmid

    #166983

    Purpose

    Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ EcRecT recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance

    Depositor

    Christopher Reisch

    Article

    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

    Insert

    RecT (recT E. coli)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pVvanCC

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSCL.140

    Plasmid

    #184995

    Purpose

    Express Cas9 in human cells

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    SpCas9
    

    Use

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    Promoter

    TetOn-3g

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. px459 VRER

    Plasmid

    #101716

    Purpose

    sgRNA/SpCas9 expression plasmid with Cas9 VRER mutations (NGCG PAM)

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VRER
    

    Use

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VRER (D1135V, G1218R, R1335E and T1337R)

    Promoter

    Available since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSDMA65

    Plasmid

    #128354

    Purpose

    Codon optimised Streptococcus pyogenes CAS9 ORF regulated by the Cryptococcus neoformans TEF1 promoter and terminator.

    Depositor

    James Fraser

    Article

    Arras et al PLoS One. 2016 Oct 6;11(10):e0164322. do

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    ACT1

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLentiCRISPRv1.mir451_KO

    Plasmid

    #164689

    Purpose

    For CRISPR knockout of miR-451 by lentiviral delivery of Cas9 and miR-451 gRNA

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Insert

    miR-451 CRISPR KO gRNA (MIR451A )
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCZGY2729

    Plasmid

    #135096

    Purpose

    Site specific insertion using CRISPR/Cas9 editing of C. elegans ChrIV

    Depositor

    Yishi Jin

    Article

    Andrusiak et al Neuron. 2019 Jul 24. pii: S0896-6273(19)

    Insert

    Hygromycin resistance
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    rps-0

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pX330-sgRNA_Drosha_4

    Plasmid

    #79863

    Purpose

    CRISPR/Cas9 DROSHA

    Depositor

    Constance Ciaudo

    Article

    Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

    Insert

    sgRNA Drosha
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

34. pX330-sgRNA_Drosha_1

    Plasmid

    #79860

    Purpose

    CRISPR/Cas9 DROSHA

    Depositor

    Constance Ciaudo

    Article

    Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

    Insert

    sgRNA Drosha
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

35. pX330-sgRNA_Drosha_2

    Plasmid

    #79861

    Purpose

    CRISPR/Cas9 DROSHA

    Depositor

    Constance Ciaudo

    Article

    Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

    Insert

    sgRNA Drosha
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

36. pX330-sgRNA_Drosha_3

    Plasmid

    #79862

    Purpose

    CRISPR/Cas9 DROSHA

    Depositor

    Constance Ciaudo

    Article

    Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

    Insert

    sgRNA Drosha
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

37. px459-Rheb sgRNA

    Plasmid

    #133768

    Purpose

    Expresses Cas9 and human Rheb sgRNA

    Depositor

    Shawn Ferguson

    Article

    Angarola et al Mol Biol Cell. 2019 Oct 15;30(22):2750-2

    Insert

    Rheb sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. hIRF-1 gRNA #409 (Sa)

    Plasmid

    #98134

    Purpose

    Expresses a guide RNA (gRNA) to target human IRF-1 promoter for the Sa-Cas9 system

    Depositor

    Hodaka Fujii

    Article

    Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

    Insert

    gRNA_hIRF1 promoter #409
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. hIRF-1 gRNA #351 (Sa)

    Plasmid

    #105283

    Purpose

    Expresses a guide RNA (gRNA) to target human IRF-1 promoter for the Sa-Cas9 system

    Depositor

    Hodaka Fujii

    Article

    Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

    Insert

    gRNA_hIRF1 promoter #351
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pDGE168

    Plasmid

    #79458

    Purpose

    recipient plasmid [multiplexing]; pDD45:Cas9, FAST, Hygro

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Insert

    ccdB (ccdB(letD) )
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    BsaI site eliminated

    Promoter

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge