280,409 results

1. pcDNA5-FRT-TO-Nsp16-HF

   Plasmid

   #157725

   Purpose

   mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp16 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nsp16-HF (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   His8-Flag

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pDEST17-UbE2G1

   Plasmid

   #15790

   Depositor

   Wade Harper

   Article

   Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

   Insert

   Ubiquitin conjugating enzyme UbE2G1 (UBE2G1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

3. pDEST17-UbE2G1

   Plasmid

   #15790

   Depositor

   Wade Harper

   Article

   Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

   Insert

   Ubiquitin conjugating enzyme UbE2G1 (UBE2G1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

4. Cerulean

   Plasmid

   #15214

   Depositor

   Dave Piston

   Article

   Rizzo et al Nat Biotechnol. 2004 Apr . 22(4):445-9.

   Insert

   monomeric Cerulean
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A206K

   Promoter

   Available since

   June 22, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Cerulean

   Plasmid

   #15214

   Depositor

   Dave Piston

   Article

   Rizzo et al Nat Biotechnol. 2004 Apr . 22(4):445-9.

   Insert

   monomeric Cerulean
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A206K

   Promoter

   Available since

   June 22, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. RFP-(SUMO)6-(SIM)10

   Plasmid

   #122027

   Purpose

   Mammalian expression plasmid for multivalent scaffold of (SUMO)6-(SIM)10 biomolecular condensates to recruit SUMO-containing client proteins.

   Depositor

   Michael Rosen

   Article

   Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

   Insert

   6 repeats of human SUMO3 and 10 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4 (PIAS2, SUMO3 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. RFP-(SUMO)6-(SIM)10

   Plasmid

   #122027

   Purpose

   Mammalian expression plasmid for multivalent scaffold of (SUMO)6-(SIM)10 biomolecular condensates to recruit SUMO-containing client proteins.

   Depositor

   Michael Rosen

   Article

   Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

   Insert

   6 repeats of human SUMO3 and 10 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4 (PIAS2, SUMO3 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Lenti-EF1alpha-VP64-dCas9-p300_Blast

   Plasmid

   #192654

   Purpose

   3rd generation lenti vector encoding VP64-dCas9-p300 with 2A Blast resistance marker

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   VP64-dCas9-p300
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A; H840A in Cas9

   Promoter

   EF1a

   Available since

   March 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-Ef1a-fDIO-GCaMP6f (AAV1)

   Viral Prep

   #128315-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV-Ef1a-fDIO-GCaMP6f (#128315). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO-GCaMP6f plasmid DNA. EF1a-driven, Flp-dependent expression of calcium sensor GCaMP6f. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Patricia Jensen

   Article

   Sciolino et al Sci Adv. 2022 Aug 19;8(33):eabn9134. doi

   Promoter

   EF1a

   Tags

   None

   Available since

   March 12, 2021

   Availability

   Academic Institutions and Nonprofits only

10. pM201

    Plasmid

    #173179

    Purpose

    dCas9-24xGCN4

    Depositor

    Hajin Kim

    Article

    Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

    Insert

    dCas9-24xGCN4
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pM201

    Plasmid

    #173179

    Purpose

    dCas9-24xGCN4

    Depositor

    Hajin Kim

    Article

    Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

    Insert

    dCas9-24xGCN4
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. eGFP-PRM-5R

    Plasmid

    #112155

    Purpose

    Mammalian expression plasmid for PRM-5R in a vector expressing eGFP.

    Depositor

    Michael Rosen

    Article

    Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

    Insert

    PRM-5R (ABL1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    contains only the PRM domain of Abl1

    Promoter

    CMV

    Available since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. eGFP-PRM-5R

    Plasmid

    #112155

    Purpose

    Mammalian expression plasmid for PRM-5R in a vector expressing eGFP.

    Depositor

    Michael Rosen

    Article

    Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

    Insert

    PRM-5R (ABL1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    contains only the PRM domain of Abl1

    Promoter

    CMV

    Available since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-CMV-SauriCas9

    Plasmid

    #135964

    Purpose

    Expresses SauriCas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-CMV-SauriCas9

    Plasmid

    #135964

    Purpose

    Expresses SauriCas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pet-15b-ultraID

    Plasmid

    #172879

    Purpose

    expresses ultraID in E. coli

    Depositor

    Julien Béthune

    Article

    Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1

    Insert

    ultraID
    

    Use

    Tags

    myc

    Expression

    Bacterial

    Mutation

    fragment ([aa2-171]) of aquifex aeolicus BirA wit…

    Promoter

    T7

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pet-15b-ultraID

    Plasmid

    #172879

    Purpose

    expresses ultraID in E. coli

    Depositor

    Julien Béthune

    Article

    Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1

    Insert

    ultraID
    

    Use

    Tags

    myc

    Expression

    Bacterial

    Mutation

    fragment ([aa2-171]) of aquifex aeolicus BirA wit…

    Promoter

    T7

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-pCMV-dSaCas9-VP64-pU6-sgRNA

    Plasmid

    #158990

    Purpose

    Vector G encodes pAAV-pCMV-dSaCas9-VP64-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in mammalian cells

    Depositor

    Chung Tin

    Article

    Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

    Insert

    dSaCas9-VP64
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    Aug. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-pCMV-dSaCas9-VP64-pU6-sgRNA

    Plasmid

    #158990

    Purpose

    Vector G encodes pAAV-pCMV-dSaCas9-VP64-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in mammalian cells

    Depositor

    Chung Tin

    Article

    Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

    Insert

    dSaCas9-VP64
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    Aug. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDONR223_SNAI2_WT

    Plasmid

    #82162

    Purpose

    Gateway Donor vector containing SNAI2 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    SNAI2 (SNAI2 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDONR223_SNAI2_WT

    Plasmid

    #82162

    Purpose

    Gateway Donor vector containing SNAI2 , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    SNAI2 (SNAI2 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. HA-SOCS1

    Plasmid

    #174571

    Purpose

    Human HA-tagged SOCS1 expression (N-term. tag) (CMV prom.)

    Depositor

    Mathew Garnett

    Article

    Coelho et al Cancer Cell. 2023 Feb 13;41(2):288-303.e

    Insert

    HA-SOCS1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGWB517

    Plasmid

    #74859

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with 4xMyc (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGWB517

    Plasmid

    #74859

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with 4xMyc (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Glycogene CRISPR Library

    Pooled Library

    #140961

    Purpose

    Targets human genes involved in cellular glycosylation.

    Depositor

    Sriram Neelamegham

    Article

    Zhu et al Glycobiology. 2020 Aug 8. pii: 5889976.

    Species

    Homo sapiens

    Use

    CRISPR and Lentiviral

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

26. CRY2olig-PR

    Plasmid

    #218917

    Purpose

    Expression of CRY2olig-mCherry fused to a codon-optimised poly-PR sequence

    Depositor

    Tatyana Shelkovnikova

    Article

    Hodgson et al iScience. 2024 Sep 14;27(10):110937. doi

    Insert

    CRY2olig-mCherry tagged poly-PR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    wild-type

    Promoter

    CMV

    Available since

    Aug. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. CRY2olig-GR

    Plasmid

    #218919

    Purpose

    Expression of CRY2olig-mCherry fused to a codon-optimised poly-GR sequence

    Depositor

    Tatyana Shelkovnikova

    Article

    Hodgson et al iScience. 2024 Sep 14;27(10):110937. doi

    Insert

    CRY2olig-mCherry tagged poly-GR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    wild-type

    Promoter

    CMV

    Available since

    Aug. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Myc-tagged delta N (dominant-negative form) human TCF4E pcDNA3

    Plasmid

    #32739

    Depositor

    Frank McCormick

    Article

    Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

    Insert

    TCF4E (Tcf7l2 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    The N-terminal deletion form (delta N) lacks amin…

    Promoter

    Available since

    Jan. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Myc-tagged delta N (dominant-negative form) human TCF4E pcDNA3

    Plasmid

    #32739

    Depositor

    Frank McCormick

    Article

    Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

    Insert

    TCF4E (Tcf7l2 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    The N-terminal deletion form (delta N) lacks amin…

    Promoter

    Available since

    Jan. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. TFORF0123

    Plasmid

    #141528

    Purpose

    Lentiviral vector for overexpressing the OLIG2 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    OLIG2 (OLIG2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. TFORF0123

    Plasmid

    #141528

    Purpose

    Lentiviral vector for overexpressing the OLIG2 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    OLIG2 (OLIG2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pK036.TRE-Flpe-WPRE (Supernova)

    Plasmid

    #85007

    Purpose

    For Flpe/FRT-based Supernova, pK036 should be used with pK068 etc.

    Depositor

    Takuji Iwasato

    Article

    Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

    Insert

    NLS-FLPe-WPRE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pK036.TRE-Flpe-WPRE (Supernova)

    Plasmid

    #85007

    Purpose

    For Flpe/FRT-based Supernova, pK036 should be used with pK068 etc.

    Depositor

    Takuji Iwasato

    Article

    Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

    Insert

    NLS-FLPe-WPRE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pYC-C

    Plasmid

    #207114

    Purpose

    HDR donor template with Puromycin resistance and mNeonGreen marker flanked by two multiple cloning sites (MCS) for insertion of left and right homology arms (L- and R-arms) for the targeted locus

    Depositor

    Yifan Cheng

    Article

    Choi et al Proc Natl Acad Sci U S A. 2023 Aug;120(3

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pYJA5

    Plasmid

    #217778

    Purpose

    The empty vector for quadruple sgRNA cloning

    Depositor

    Adriano Aguzzi

    Article

    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038

    Type

    Empty backbone

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    Puromycin resistance gene, T2A, and TagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    PGK, CMV, U6

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAAV_CMV::hTfR-GFP

    Plasmid

    #199714

    Purpose

    Expresses a chimera of human transferrin receptor (hTfR) and GFP in mammalian cells.

    Depositor

    Alice Ting

    Article

    Roh et al J Am Chem Soc. 2023 May 10;145(18):10220

    Insert

    hTfR-GFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCW-LIC-N8C_Fab3a

    Plasmid

    #203689

    Purpose

    Expresses N8C_Fab3a

    Depositor

    Brenda Schulman

    Article

    Henneberg et al Nat Chem Biol. 2023 Dec;19(12):1513-1523

    Insert

    N8C_Fab3a
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. PMXS-NDI1

    Plasmid

    #72876

    Purpose

    expresses NDI1 in mammalian cells

    Depositor

    David Sabatini

    Article

    Birsoy et al Nature. 2014 Apr 3;508(7494):108-12. doi

    Insert

    NDI1 (NDI1 Budding Yeast)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. PMXS-NDI1

    Plasmid

    #72876

    Purpose

    expresses NDI1 in mammalian cells

    Depositor

    David Sabatini

    Article

    Birsoy et al Nature. 2014 Apr 3;508(7494):108-12. doi

    Insert

    NDI1 (NDI1 Budding Yeast)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pDONR223_SNAI1_WT

    Plasmid

    #82164

    Purpose

    Gateway Donor vector containing SNAI1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    SNAI1 (SNAI1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge