Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

163,421 results

Showing: 1081 - 1120 of 163421 results

  3. pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA (AAV8)

    Viral Prep
    #154867-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA (#154867). In addition to the viral particles, you will also receive purified pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA plasmid DNA. Syn-driven, Flp-dependent hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ulrik Gether
    Article
    Runegaard et al eNeuro. 2018 Jun 18;5(3). pii: eN-NWR-03
    Promoter
    Human Synapsin-1
    Tags
    mCherry (Flp-dependent)
    Available since
    Aug. 19, 2022
    Availability
    Academic Institutions and Nonprofits only

  6. pUCmini-iCAP-AAV.CAP-B10

    Plasmid
    #175004
    Purpose
    non-standard AAV2 rep-AAV.CAP-B10 cap plasmid with AAV cap expression controlled by a tTA-TRE amplifcation system
    Depositor
    Viviana Gradinaru
    Article
    Goertsen et al Nat Neurosci. 2022 Jan;25(1):106-115. do
    Insert
    Synthetic construct isolate AAV.CAP-B10 VP1 gene
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    7 amino acid substitution between VP1 452 and VP1…
    Promoter
    p41
    Available since
    Nov. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    175004-plasmid-map-sequence-id-347131

    Enlarge

  7. pAAV.Syn.NES-jRGECO1a.WPRE.SV40 (AAV1)

    Viral Prep
    #100854-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV.Syn.NES-jRGECO1a.WPRE.SV40 (#100854). In addition to the viral particles, you will also receive purified pAAV.Syn.NES-jRGECO1a.WPRE.SV40 plasmid DNA. Synapsin-driven GECO1a calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project, Douglas Kim
    Article
    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:
    Promoter
    Syn
    Tags
    None
    Available since
    April 6, 2018
    Availability
    Academic Institutions and Nonprofits only

  8. LRP1A-c004

    Plasmid
    #205112
    Purpose
    LRP1 protein expression
    Depositor
    Paul Brennan
    Insert
    LRP1 (LRP1 Human)
    Use
    Tags
    AviTag and GP64sp-6His-TEV
    Expression
    Insect
    Mutation
    WT
    Promoter
    Available since
    Oct. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    205112-plasmid-map-sequence-id-412366

    Enlarge

  11. pGP-AAV-syn-FLEX-jGCaMP8f-WPRE (AAV9)

    Viral Prep
    #162379-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pGP-AAV-syn-FLEX-jGCaMP8f-WPRE (#162379). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP8f-WPRE plasmid DNA. Syn-driven, Cre-dependent expression of the ultrafast calcium sensor, GCaMP8f. These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project
    Article
    Zhang et al Janelia Research Campus, Online Resource
    Promoter
    Syn
    Tags
    None
    Available since
    June 22, 2021
    Availability
    Academic Institutions and Nonprofits only

  12. TETv4

    Plasmid
    #167983
    Purpose
    Expresses TETv4 (TET1CD-XTEN80-dCas9-3xNLS-P2A-BFP) downstream of the CAG promoter
    Depositor
    Luke Gilbert
    Article
    Nuñez et al Cell. 2021 Apr 7. pii: S0092-8674(21)003
    Insert
    TETv4 (TET1CD-XTEN80-dCas9)
    Use
    CRISPR and Synthetic Biology
    Tags
    3xNLS, TET1 catalytic domain, and tagBFP
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    May 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167983-plasmid-map-sequence-id-418937

    Enlarge

  14. pAAV-hSyn-DIO-hM4D(Gi)-mCherry (AAV8)

    Viral Prep
    #44362-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-hSyn-DIO-hM4D(Gi)-mCherry (#44362). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM4D(Gi)-mCherry plasmid DNA. Syn-driven, Cre-dependent, hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Article
    Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d
    Promoter
    Syn
    Tags
    mCherry (Cre-dependent)
    Available since
    Sept. 28, 2016
    Availability
    Academic Institutions and Nonprofits only

  17. pBBR1MCS-2

    Plasmid
    #85168
    Purpose
    Mobilisable shuttle and expression vector. Replicates in many Gram-negative bacteria. Has multiple cloning site with blue/white selection function. Cloned genes driven by derepressed lac promoter.
    Depositor
    Kenneth Peterson
    Article
    Kovach et al Gene. 1995 Dec 1;166(1):175-6.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Constitutive (derepressed P-lac)
    Available since
    Nov. 8, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    85168-plasmid-map-sequence-id-372970

    Enlarge

  20. T7-VEE-GFP

    Plasmid
    #58977
    Purpose
    expression of GFP using a self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon
    Depositor
    Steven Dowdy
    Article
    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.
    Insert
    EGFP
    Use
    Venezuelan equine encephalitis (vee) virus rna re…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    26S subgenomic promoter
    Available since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    58977-plasmid-map-sequence-id-419580

    Enlarge

  22. pGP-AAV-syn-FLEX-jGCaMP8f-WPRE (AAV1)

    Viral Prep
    #162379-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pGP-AAV-syn-FLEX-jGCaMP8f-WPRE (#162379). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP8f-WPRE plasmid DNA. Syn-driven, Cre-dependent expression of the ultrafast calcium sensor, GCaMP8f. These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project
    Article
    Zhang et al Janelia Research Campus, Online Resource
    Promoter
    Syn
    Tags
    None
    Available since
    May 18, 2021
    Availability
    Academic Institutions and Nonprofits only

  23. BPK1520

    Plasmid
    #65777
    Purpose
    Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    SpCas9 gRNA backbone, without spacer sequence
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65777-plasmid-map-sequence-id-316938

    Enlarge

  25. pEVOL-pAzF

    Plasmid
    #31186
    Purpose
    tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in E coli response to the amber codon, TAG
    Depositor
    Peter Schultz
    Article
    Chin et al J Am Chem Soc. 2002 Aug 7;124(31):9026-7
    Insert
    M.j. p-azidohenylalanine RS (2 copies +tRNA)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y32T, E107N, D158P, I159L, L162Q, D286R
    Promoter
    Available since
    Dec. 9, 2011
    Availability
    Academic Institutions and Nonprofits only
    31186-plasmid-map-sequence-id-334781

    Enlarge

  27. pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3 (AAV9)

    Viral Prep
    #108912-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3 (#108912). In addition to the viral particles, you will also receive purified pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3 plasmid DNA. CAG-driven, Cre-dependent ChroME-ST-P2A-H2B-mRuby3 expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Hillel Adesnik
    Article
    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/
    Promoter
    CAG
    Tags
    mRuby3 (Cre-dependent)
    Available since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only

  30. sgRNA_expression_vector

    Plasmid
    #210212
    Purpose
    An empty gRNA expression vector to clone U6 promoter driven sgRNAs with gRNA scaffold and with co-expression of DsRed. sgRNA can be cloned by Golden Gate Assembly or restriction digestion using BbsI.
    Depositor
    Albert Jeltsch
    Article
    Rajaram et al Methods Mol Biol. 2024;2842:179-192. doi
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    U6
    Available since
    Dec. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    210212-plasmid-map-sequence-id-416400

    Enlarge

  31. pAAV-GFAP104-mCherry (AAV5)

    Viral Prep
    #58909-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-GFAP104-mCherry (#58909). In addition to the viral particles, you will also receive purified pAAV-GFAP104-mCherry plasmid DNA. GFAP104-driven mCherry expression control. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Article
    Perea et al Nat Commun. 2014;5:3262. doi: 10.1038/nc
    Promoter
    GFAP
    Tags
    mCherry
    Available since
    Feb. 27, 2019
    Availability
    Academic Institutions and Nonprofits only

  32. pAAV-CaMKIIa-hM4D(Gi)-mCherry (AAV9)

    Viral Prep
    #50477-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-CaMKIIa-hM4D(Gi)-mCherry (#50477). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hM4D(Gi)-mCherry plasmid DNA. CaMKIIa-driven hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal silencing. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    CaMKII
    Tags
    mCherry
    Available since
    Jan. 26, 2021
    Availability
    Academic Institutions and Nonprofits only

  33. pUCmini-iCAP-AAV.CAP-B22

    Plasmid
    #175005
    Purpose
    non-standard AAV2 rep-AAV.CAP-B22 cap plasmid with AAV cap expression controlled by a tTA-TRE amplifcation system
    Depositor
    Viviana Gradinaru
    Article
    Goertsen et al Nat Neurosci. 2022 Jan;25(1):106-115. do
    Insert
    Synthetic construct isolate AAV.CAP-B22 VP1 gene
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    7 amino acid substitution between VP1 452 and VP1…
    Promoter
    p41
    Available since
    Nov. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    175005-plasmid-map-sequence-id-347133

    Enlarge

  39. pGP-AAV-syn-jGCaMP8s-WPRE (AAV9)

    Viral Prep
    #162374-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pGP-AAV-syn-jGCaMP8s-WPRE (#162374). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP8s-WPRE plasmid DNA. Syn-driven expression of calcium sensor GCaMP8s (more sensitive). These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project
    Article
    Zhang et al Janelia Research Campus, Online Resource
    Promoter
    Syn
    Tags
    None
    Available since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 1081 - 1120 of 163421 results