183,825 results

1. pAAV-Syn1-tTA

   Plasmid

   #104109

   Purpose

   AAV-mediated expression of tTA under the Syn1 promoter.

   Depositor

   Takeshi Imai

   Article

   Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

   Insert

   tTA2
   

   Use

   AAV

   Promoter

   hSyn1

   Available Since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. V245 pCEP-4HA B56alpha

   Plasmid

   #14532

   Depositor

   David Virshup

   Article

   Seeling et al Science. 1999 Mar 26. 283(5410):2089-91.

   Insert

   PPP2R5A B56 alpha regulatory subunit of PP2A (PPP2R5A Human)
   

   Tags

   4 HA

   Expression

   Mammalian

   Available Since

   March 23, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. V245 pCEP-4HA B56alpha

   Plasmid

   #14532

   Depositor

   David Virshup

   Article

   Seeling et al Science. 1999 Mar 26. 283(5410):2089-91.

   Insert

   PPP2R5A B56 alpha regulatory subunit of PP2A (PPP2R5A Human)
   

   Tags

   4 HA

   Expression

   Mammalian

   Available Since

   March 23, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL0_9 [pUB110 ORI]

   Plasmid

   #198924

   Purpose

   Level 0 part

   Depositor

   Charlie Gilbert, Cultivarium Tools, Nili Ostrov

   Article

   Gilbert et al bioRxiv 2023.05.19.541510

   Insert

   pUB110 ORI
   

   Use

   Synthetic Biology

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEAK10-His-Myc-Kal9

   Plasmid

   #25441

   Depositor

   Betty Eipper

   Article

   Johnson et al J Biol Chem. 2000 Jun 23. 275(25):19324-

   Insert

   Kal9
   

   Tags

   His and Myc

   Expression

   Mammalian

   Available Since

   July 15, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pEAK10-His-Myc-Kal9

   Plasmid

   #25441

   Depositor

   Betty Eipper

   Article

   Johnson et al J Biol Chem. 2000 Jun 23. 275(25):19324-

   Insert

   Kal9
   

   Tags

   His and Myc

   Expression

   Mammalian

   Available Since

   July 15, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pEAK10-His-Myc-Kal5

   Plasmid

   #25440

   Depositor

   Betty Eipper

   Article

   Johnson et al J Biol Chem. 2000 Jun 23. 275(25):19324-

   Insert

   Kal5
   

   Tags

   His and Myc

   Expression

   Mammalian

   Available Since

   July 15, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pEAK10-His-Myc-Kal5

   Plasmid

   #25440

   Depositor

   Betty Eipper

   Article

   Johnson et al J Biol Chem. 2000 Jun 23. 275(25):19324-

   Insert

   Kal5
   

   Tags

   His and Myc

   Expression

   Mammalian

   Available Since

   July 15, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pLVX-EF1a-Calr-KDEL-IRES-Puromycin

   Plasmid

   #134664

   Purpose

   Expresses EGFP-tagged endoplasmic reticulum marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   calreticulin (CALR Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 18-413

   Promoter

   EF1a

   Available Since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. RFP-HA-DAT

    Plasmid

    #90265

    Purpose

    Expresses RFP-HA-DAT in mammalian cells

    Depositor

    Alexander Sorkin

    Article

    Caltagarone et al Mol Cell Neurosci. 2015 Sep;68:120-30. d

    Insert

    DAT (dopamine transporter) (SLC6A3 Human)
    

    Expression

    Mammalian

    Mutation

    inserted HA tag (see YFP-HA-DAT)

    Promoter

    CMV

    Available Since

    May 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. SPARC-bio-His

    Plasmid

    #52078

    Purpose

    Expresses full-length Secreted protein acidic and rich in cysteine precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    SPARC (SPARC Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. TFORF3008

    Plasmid

    #144484

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    CDX2 (CDX2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGMC00025 (aka pCW-dCas9-Blast)

    Plasmid

    #195316

    Purpose

    Dox inducible lentiviral dead Cas9

    Depositor

    Raj Chari

    Insert

    dead Cas9
    

    Use

    CRISPR and Lentiviral

    Expression

    Bacterial and Mammalian

    Mutation

    D10A; H840A

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTO_CHMP4B_LAP

    Plasmid

    #101850

    Purpose

    Tet-inducible expression of human CHMP4B with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration

    Depositor

    Daniel Gerlich

    Article

    Mierzwa et al Nat Cell Biol. 2017 Jul;19(7):787-798. d

    Insert

    Charged Multivesicular Body Protein 4B (CHMP4B Human)
    

    Use

    Gateway cloning, flp-frt recombination, tet-induc…

    Tags

    Localization and Affinity Purification (LAP) tag

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    Dec. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pICH47742::2x35S-5’UTR-hCas9(STOP)-NOST

    Plasmid

    #49771

    Purpose

    Level 1 hCas9 module

    Depositor

    Sophien Kamoun

    Article

    Belhaj et al Plant Methods. 2013 Oct 11;9(1):39.

    Insert

    35Sp::hCas9
    

    Use

    CRISPR

    Available Since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRosetta_v2

    Plasmid

    #136477

    Purpose

    Lentiviral GFP Reporter assay for determining appropriate dose of puromycin, blasticidin, and hygromycin

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

    Insert

    HygroR-T2A-BlastR-P2A-PuroR-F2A-EGFP
    

    Use

    Lentiviral

    Promoter

    PGK

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pC.HA Vav2

    Plasmid

    #14554

    Depositor

    Joan Brugge

    Article

    Moores et al Mol Cell Biol. 2000 Sep . 20(17):6364-73

    Insert

    Vav2 (VAV2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    April 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. pC.HA Vav2

    Plasmid

    #14554

    Depositor

    Joan Brugge

    Article

    Moores et al Mol Cell Biol. 2000 Sep . 20(17):6364-73

    Insert

    Vav2 (VAV2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    April 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMSCV-IRES-YFP II (pMIY II)

    Plasmid

    #52108

    Purpose

    a bicistronic IRES-YFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP

    Depositor

    Dario Vignali

    Article

    Holst et al Nat Protoc. 2006;1(1):406-17.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    IRES-EYFP

    Expression

    Mammalian

    Available Since

    March 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. 12xMoonTag-12xSunTag-kif18b-24xPP7

    Plasmid

    #128606

    Purpose

    Expresses 12xgp41 peptide array (MoonTag peptide array), followed by 12xgcn4 peptide array (SunTag peptide array), kif18b gene and 24 repeats of PP7 hairpins in 3’ UTR.

    Depositor

    Marvin Tanenbaum

    Article

    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

    Inserts

    12xMoonTag-12xSunTag
    kinesin family member 18b (KIF18B Human)
    

    Expression

    Mammalian

    Promoter

    Pcmv

    Available Since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. pAAV-EF1a-DIO-GRAB_g5-HT3.0

    Plasmid

    #208726

    Purpose

    Expresses the green 5-HT sensor GRAB_g5-HT3.0 in neurons in the presence of Cre recombinase

    Depositor

    Yulong Li

    Article

    Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

    Insert

    Green fluorescent 5-HT sensor GRAB_g5-HT3.0
    

    Use

    AAV

    Promoter

    EF1a

    Available Since

    Oct. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. pmNeonGreenHO-G

    Plasmid

    #127912

    Purpose

    a mammalian expression backbone vector for humanized mNeonGreen

    Depositor

    Isei Tanida

    Article

    Tanida-Miyake et al PLoS One. 2018 Jan 17;13(1):e0191108. do

    Insert

    humanized mNeonGreen
    

    Tags

    mNeonGreen

    Expression

    Mammalian

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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23. pEGFP/golgin-160

    Plasmid

    #180630

    Purpose

    Expression of WT golgin-160

    Depositor

    Carolyn Machamer

    Article

    Mancini et al J Cell Biol. 2000 May 1;149(3):603-12. d

    Insert

    golgin-160 (GOLGA3 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pU6-As-crRNA

    Plasmid

    #78956

    Purpose

    Cloning vector for expression of AsCpf1 crRNA. It contains BsmB1 site for cloning.

    Depositor

    Jin-Soo Kim

    Article

    Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 promoter

    Available Since

    June 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA5-EGFP-AID-BubR1

    Plasmid

    #47330

    Purpose

    for plasmid integration using the Flp-In system. AID at N-terminal

    Depositor

    Don Cleveland

    Article

    Holland et al Proc Natl Acad Sci U S A. 2012 Dec 4;109

    Insert

    BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B Human)
    

    Tags

    AID and GFP

    Expression

    Mammalian

    Mutation

    siRNA resistant

    Available Since

    Sept. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. FUGW-Golgi-TurboID

    Plasmid

    #200964

    Purpose

    3rd gen lentiviral plasmid for expressing golgi localized TurboID with EGFP

    Depositor

    Keriann Backus

    Article

    Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

    Insert

    Golgi-TurboID
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLPT41

    Plasmid

    #85524

    Purpose

    Titration sponge for TetR

    Depositor

    Johan Paulsson

    Article

    Potvin-Trottier et al Nature. 2016 Oct 12;538(7626):514-517. d

    Type

    Empty backbone

    Use

    Synthetic Biology

    Promoter

    PLtet

    Available Since

    April 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40

    Plasmid

    #100054

    Purpose

    AAV expression of humanized ChR2 with H134R mutation fused to mCherry driven by CAG promoter for optogenetic activation

    Depositor

    Karl Deisseroth

    Has Service

    AAV1, AAV2, AAV5, and AAV9

    Insert

    channelrhodopsin-2
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    H134R mutation in ChR2

    Promoter

    CAG

    Available Since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3 Myc2- Hs TTP WT

    Plasmid

    #107008

    Purpose

    Expresses epitope-tagged wild-type human TTP

    Depositor

    Julian Downward

    Article

    Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

    Insert

    tristetraprolin (ZFP36 Human)
    

    Tags

    Double Myc peptide epitope tag

    Expression

    Mammalian

    Available Since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3-Casp2-Flag

    Plasmid

    #11811

    Depositor

    Guy Salvesen

    Insert

    Caspase-2 (CASP2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    May 12, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3-Casp2-Flag

    Plasmid

    #11811

    Depositor

    Guy Salvesen

    Insert

    Caspase-2 (CASP2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    May 12, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pUC57 (Gent)

    Plasmid

    #54338

    Depositor

    Martin Parniske

    Article

    Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

    Type

    Empty backbone

    Use

    Cloning vector

    Available Since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pTipi2.1-AZU_SP

    Plasmid

    #229144

    Purpose

    Allows for expression of recombinant antibodies. Includes human azurocidin signal peptide.

    Depositor

    Institute for Protein Innovation (IPI)

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Jan. 9, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

34. pLV_Zika_NS5_Flag

    Plasmid

    #79639

    Purpose

    Third generation Self-inactivating lentivector expressing NS5 from Zika virus

    Depositor

    Vaithi Arumugaswami

    Insert

    NS5
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    EF1

    Available Since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. GFP-Tollip

    Plasmid

    #178050

    Purpose

    GFP fused to human Tollip cDNA. Localisation and expression in mammalian cells.

    Depositor

    David Tumbarello

    Article

    Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi:

    Insert

    Toll-Interacting Protein (TOLLIP Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pJSC111 - Bacterial expression plasmid for SpCas9-HF1 variant

    Plasmid

    #101209

    Purpose

    Bacterial expression plasmid for SpCas9-HF1 variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant N497A/R661A/Q695A/Q926A
    

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    N497A, R661A, Q695A and Q926A

    Promoter

    T7

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pHH0103_RAD51_Rad51

    Plasmid

    #110031

    Purpose

    Protein expression and purification of RAD51_Rad51

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    RAD51_Rad51 (RAD51 Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pTipi2.1

    Plasmid

    #225877

    Purpose

    Allows for expression of recombinant antibodies

    Depositor

    Institute for Protein Innovation (IPI)

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Oct. 23, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

39. pB_tetO7_sfGFP

    Plasmid

    #196616

    Purpose

    Plasmid for integrating tetO7-controlled sfGFP into the HO region in yeast

    Depositor

    Matthias Heinemann

    Article

    Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

    Insert

    Superfolder GFP
    

    Expression

    Yeast

    Promoter

    tetO7 promoter

    Available Since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. UbG76V-eGFP-V5His_20aa

    Plasmid

    #23970

    Depositor

    Nico Dantuma

    Article

    Beskow et al J Mol Biol. 2009 Dec 11. 394(4):732-46.

    Insert

    Ub-G76V-eGFP-V5HIS_20aa
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    G76V mutation in Ubiquitin. Ub fused to N-termin…

    Available Since

    March 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge