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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,137 results for: NSI

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Showing: 11241 - 11280 of 12137 results

  1. Luc-PS9

    Plasmid
    #177942
    Purpose
    Transient mammalian expression of Luc2 (firefly luciferase) along with SARS-CoV-2 sequence 20080-21171 (PS9) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    Luc2 (ORF1ab )
    Expression
    Mammalian
    Available Since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    177942-plasmid-map-sequence-id-348206

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  3. Luc-T20

    Plasmid
    #177941
    Purpose
    Transient mammalian expression of Luc2 (firefly luciferase) along with SARS-CoV-2 sequence 20080-22222 (T20) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    Luc2 (ORF1ab )
    Expression
    Mammalian
    Available Since
    Nov. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    177941-plasmid-map-sequence-id-348207

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  4. pDule-3-nitroTyrosine (5B)

    Plasmid
    #85498
    Purpose
    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Cooley et al Biochemistry. 2014 Apr 1;53(12):1916-24.
    Insert
    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32H H70C D158S I159A L162R
    Promoter
    lpp (constitutive)
    Available Since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85498-plasmid-map-sequence-id-169862

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  5. pORTMAGE-2

    Plasmid
    #72677
    Purpose
    Expresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Ap resistance marker.
    Depositor
    Csaba Pál
    Article
    Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p
    Insert
    mutL E32K
    Tags
    none
    Expression
    Bacterial
    Mutation
    E32K mutation conferring dominant mutator phenoty…
    Promoter
    pL promoter
    Available Since
    Feb. 18, 2016
    Availability
    Academic Institutions and Nonprofits only
    72677-plasmid-map-sequence-id-457485

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  6. pRSF-MjCouRS

    Plasmid
    #229066
    Purpose
    tRNA synthetase/tRNA pair for the in vivo incorporation of (7-hydroxy-4-coumarin-yl) ethylglycine (Hco), into proteins in E. coli in response to the amber (TAG) codon
    Depositor
    Thomas Huber
    Article
    Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024
    Insert
    Methanococcus jannaschii tRNA synthetase
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    229066-plasmid-map-sequence-id-459819

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  9. pLM1110_LEU2

    Plasmid
    #168460
    Purpose
    Big-IN Payload yeast assembly vector. Encodes LEU2 yeast selectable marker and components for bacterial copy number induction. Contains a mammalian transient (backbone) GFP-T2A-BSD selection cassette.
    Depositor
    Jef Boeke
    Article
    Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118
    Insert
    mRFP1
    Use
    Synthetic Biology; Yeast assembly vector for big-…
    Available Since
    June 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    168460-plasmid-map-sequence-id-365349

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  10. pYPQ-CBE-Cas9n-Act3.0

    Plasmid
    #178955
    Purpose
    It consists of a Cas9 nickase (D10A) fused with a cytidine deaminase (A3A/Y130F) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous C to T conversion and gene activation.
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:
    Insert
    hA3A-zCas9-UGI-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Expression
    Plant
    Available Since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    178955-plasmid-map-sequence-id-458203

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  11. pYPQ-CBE-SpRYn-Act3.0

    Plasmid
    #178958
    Purpose
    It consists of a SpRY nickase (D10A) fused with a cytidine deaminase (A3A/Y130F) and MS2 -SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous C to T conversion and gene activation.
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:
    Insert
    hA3A-SpRY-UGI-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Expression
    Plant
    Available Since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    178958-plasmid-map-sequence-id-354326

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  14. CMV-NLS-R-GECO

    Plasmid
    #32462
    Purpose
    Mammalian expression of nucleus-targeting red fluorescent genetically encoded Ca2+ indicator for optical imaging
    Depositor
    Robert Campbell
    Article
    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.
    Insert
    R-GECO1.0
    Tags
    NLS sequence DPKKKRKV
    Expression
    Mammalian
    Mutation
    Substitutions relative to the mApple-derived anal…
    Promoter
    CMV
    Available Since
    Sept. 29, 2011
    Availability
    Academic Institutions and Nonprofits only
    32462-plasmid-map-sequence-id-201367

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  16. pDule-Tet2.0

    Plasmid
    #85496
    Purpose
    Plasmid for incorporating the non-canonical amino acid Tetrazine 2.0 with the Mj Tet2.0 synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Blizzard et al J Am Chem Soc. 2015 Aug 19;137(32):10044
    Insert
    Tetrazine2.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32G L65Q F108S Q109D D158S L162N
    Promoter
    lpp (constitutive)
    Available Since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85496-plasmid-map-sequence-id-169865

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  19. pBpaRS3tRNA

    Plasmid
    #197102
    Purpose
    To express the p-benzoylphenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into p-benzoylphenylalanine.
    Depositor
    Kensaku Sakamoto
    Article
    Sato et al Biochemistry. 2011 Jan 18;50(2):250-7. d
    Insert
    TyrRS variant, amber suppressor tRNA
    Expression
    Bacterial
    Mutation
    Gly32, Ser107, Thr158, Ser159, Arg286 (TyrRS)
    Available Since
    June 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    197102-plasmid-map-sequence-id-388022

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  24. pAcBac1.tR4-AcdRS82

    Plasmid
    #174101
    Purpose
    Expression of orthogonal Mb tRNA and Acd82 tRNA synthetase for incorporation of acridone in eukaryotic cells.
    Depositor
    Ryan Mehl
    Article
    Jones et al Chem Sci. 2021 Aug 3;12(36):11955-11964.
    Insert
    M. barkeri tRNA synthetase for acridone82
    Expression
    Mammalian
    Mutation
    N311S, C313G, V366A, W382T
    Promoter
    CMV
    Available Since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    174101-plasmid-map-sequence-id-345718

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  25. pMXs-IP- SCAT3.2

    Plasmid
    #172497
    Purpose
    SCAT3.2 is fluorescent reporter (FRET probe) for cell survival/apoptosis with Caspase-3 sensitive sequence which can be introduced to mammalian cells by retrovirus vector.
    Depositor
    David Scadden
    Article
    Oki et al Nat Commun. 2021 Jan 11;12(1):245. doi:
    Insert
    Amcyan
    Use
    Retroviral
    Tags
    mVenus
    Expression
    Mammalian
    Promoter
    MLV LTR
    Available Since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172497-plasmid-map-sequence-id-340999

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  26. HSC1-HS4-D4Z4c-GiP

    Plasmid
    #58541
    Purpose
    Derived from HSC1-GiP where 2 copies of the HS4 insulator core inside the 3'LTR and the D4Z4-c insulator, are combined upstream of the EF1 alpha promoter
    Depositor
    James Ellis
    Article
    Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1
    Inserts
    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    D4Z4 repeat, partial
    Use
    Retroviral
    Expression
    Mammalian
    Promoter
    Human EF1-alpha
    Available Since
    Sept. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    58541-plasmid-map-sequence-id-90716

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  27. pYPQ-ABE-Cas9n-Act3.0

    Plasmid
    #178956
    Purpose
    It consists of a Cas9 nickase (D10A) fused with an adenine deaminase (ABE8e) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous A to G conversion and gene activation.
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:
    Insert
    ABE8e-zCas9-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Expression
    Plant
    Available Since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    178956-plasmid-map-sequence-id-354328

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  32. pAL113

    Plasmid
    #22471
    Purpose
    Expresses N-terminal domains of PhyB (with Y276H mutation), fused to mCFP and HRas C terminal CAAX palmitoylation sequence. For red-light-induced binding to PIF6.
    Depositor
    Christopher Voigt
    Article
    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.
    Insert
    PhyB NT (PHYB Mustard Weed, Mouse)
    Use
    Synthetic Biology
    Tags
    10aaLinker-mCFP-HrasCT
    Expression
    Mammalian
    Mutation
    Y276H
    Promoter
    CMV
    Available Since
    Nov. 5, 2009
    Availability
    Academic Institutions and Nonprofits only
    22471-plasmid-map-sequence-id-10325

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  36. SiriusGFP

    Plasmid
    #123201
    Purpose
    We have developed a variant of eGFP, SiriusGFP, that shows over a two fold increase in photostability with utility in methods requiring sustained or high intensity excitation as in 4D confocal or SIM.
    Depositor
    Joseph Santos-Sacchi
    Article
    Zhong et al J Neurosci Methods. 2019 Feb 1;313:68-76
    Insert
    SiriusGFP
    Use
    Mouse Targeting
    Tags
    N/A
    Expression
    Bacterial and Mammalian
    Mutation
    eGFP-S30R/Y39N/F99S/N105T/S147R/M153T/V163A/S205V…
    Promoter
    CMV
    Available Since
    March 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    123201-plasmid-map-sequence-id-239192

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  38. pDule2-Tet2.0

    Plasmid
    #85497
    Purpose
    Plasmid for incorporating the non-canonical amino acid Tetrazine 2.0 with the Mj Tet2.0 synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Blizzard et al J Am Chem Soc. 2015 Aug 19;137(32):10044
    Insert
    Tetrazine2.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Expression
    Bacterial
    Mutation
    Y32G L65Q F108S Q109D D158S L162N
    Promoter
    lpp (constitutive)
    Available Since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85497-plasmid-map-sequence-id-169854

    Enlarge

  40. pYPQ-ABE-SpRYn-Act3.0

    Plasmid
    #178959
    Purpose
    It consists of a SpRY nickase (D10A) fused with an adenine deaminase (ABE8e) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous A to G conversion and gene activation.
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:
    Insert
    ABE8e-SpRY-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Expression
    Plant
    Available Since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    178959-plasmid-map-sequence-id-354322

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Showing: 11241 - 11280 of 12137 results