Addgene

We narrowed to 2,371 results for: 683

Showing: 1121 - 1160 of 2371 results

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pH6HTC_IGF2BP2a-Halo

Plasmid

#175309

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

IGF2BP2 (IGF2BP2 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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CAG:: mCherry-2a-hM4Dnrxn

Plasmid

#52523

Purpose

Co-expresses hM4Dnrxn and a red fluorescent label

Depositor

Scott Sternson

Article

Stachniak et al Neuron. 2014 May 21;82(4):797-808. doi:

Insert

mCherry-2a-hM4Dnrxn

Use

Tags

2xHA

Expression

Mammalian

Mutation

Promoter

CAg

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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Halo-OPTN

Plasmid

#187900

Purpose

Mammalian cell expression of Halo-tagged OPTN

Depositor

Erika Holzbaur

Article

Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

Insert

OPTN (OPTN Human)

Use

Tags

Halo

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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YFP-Cdc42

Plasmid

#11392

Depositor

Joel Swanson

Article

Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

Insert

cell division cycle 42 (CDC42 Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 21, 2007

Availability

Academic Institutions and Nonprofits only

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pH6HTC_FSCN1-Halo

Plasmid

#175343

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

FSCN1 (FSCN1 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCI-Neo-His-Pitaire-Myc

Plasmid

#26066

Depositor

Jonathon Pines

Article

Ko et al J Cell Sci. 2001 Jul . 114(Pt 14):2591-6

Insert

Pitaire (CDK12 Human)

Use

Tags

His and Myc

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2010

Availability

Academic Institutions and Nonprofits only
pEB2_mScarletI_mVenusNB-MrkH

Plasmid

#182291

Purpose

expresses c-di-GMP biosensor in E. coli

Depositor

Howard Berg

Article

Vrabioiu et al Proc Natl Acad Sci U S A. 2022 Feb 8;119

Insert

mVenusNB-MrkH

Use

Tags

mVenusNB internal fusion to MrkH

Expression

Bacterial

Mutation

Promoter

proC

Available since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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Hip1R-tDimerRFP-N1

Plasmid

#27700

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

Hip1R (Hip1r Mouse)

Use

Tags

tDimer-RFP

Expression

Mammalian

Mutation

Promoter

Available since

May 4, 2011

Availability

Academic Institutions and Nonprofits only
pD474N-EGFP

Plasmid

#68847

Purpose

mutated OPTN was inserted into pEGFP-N1 backbone

Depositor

Beatrice Yue

Article

Turturro et al Springerplus. 2014 Feb 19;3:99. doi: 10.

Insert

optineurin (OPTN Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

D474N (not linked to any diseases)

Promoter

CMV

Available since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pR96L-EGFP

Plasmid

#68846

Purpose

mutated OPTN was inserted into pEGFP-N1 backbone

Depositor

Beatrice Yue

Article

Turturro et al Springerplus. 2014 Feb 19;3:99. doi: 10.

Insert

optineurin (OPTN Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

R96L, associated with ALS

Promoter

CMV

Available since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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HA-TRIM28deltaBCC

Plasmid

#124954

Purpose

Mammalian expression of Human TRIM28 with deleted Bbox and CC domains

Depositor

Michelle Barton

Article

Li et al Oncogene. 2017 May 25;36(21):2991-3001.

Insert

hTRIM28 (TRIM28 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Deletion of Bbox and CC domains

Promoter

Available since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
mcherry-Myo 9b

Plasmid

#134918

Purpose

Expression of rat Myo 9b (Myr 5) in mammalian cells.

Depositor

Martin Bähler

Article

Hemkemeyer et al J Biol Chem. 2021 Jan-Jun;296:100136. do

Insert

Myo 9b (rat) with 3' UTR (Myo9b Rat)

Use

Tags

mcherry

Expression

Mammalian

Mutation

Promoter

CMV IE

Available since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pIRES RED/SP M87C448Y

Plasmid

#89324

Purpose

Expression of RFP and M87C448Y spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

Insert

spastin (SPAST Human)

Use

Tags

Expression

Mammalian

Mutation

1-460 deleted

Promoter

CMV

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pICSL90002

Plasmid

#68261

Purpose

Level 0 Golden Gate Part: Promoter U6-26 (Arabidopsis thaliana)

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter: U6-26 (Arabidopsis thaliana)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-mROCK1-delta2

Plasmid

#101290

Purpose

Mammalian expression of Rock1-GFP for the study of localisation signaling.

Depositor

Alpha Yap

Article

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Insert

Rock1 (Rock1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

1-727 aa

Promoter

CMV

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pGem-PgpdA-Cre-TtrpC

Plasmid

#168784

Purpose

Helper vector for the transient expression of Cre recombinase in filamentous fungi. Not replicative in fungi.

Depositor

Yit Heng Chooi

Article

Roux et al ACS Synth Biol. 2022 Feb 16. doi: 10.102

Insert

Cre

Use

Cre/Lox and Synthetic Biology; Expression in fila…

Tags

Expression

Mutation

Promoter

PgpdA

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-mROCK1-delta1

Plasmid

#101289

Purpose

Mammalian expression of Rock1-GFP for the study of localisation signaling.

Depositor

Alpha Yap

Article

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Insert

Rock1 (Rock1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

1-375 aa

Promoter

CMV

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
dFP-mini

Plasmid

#120543

Purpose

Expresses dFP biliverdin-binding fluorescence-activating mini 10 KDa protein in bacteria.

Depositor

Brian Chow

Article

Sheehan et al Biochemistry. 2018 Nov 28. doi: 10.1021/

Insert

de novo fluorescence-activating protein mini (dFP-mini)

Use

Tags

6xHis - TEV cleavage

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFPC3-C2cd3

Plasmid

#128871

Purpose

For mammalian expression of mouse C2cd3

Depositor

Aimin Liu

Article

Hoover et al Development. 2008 Dec;135(24):4049-58. d

Insert

C2cd3 (C2cd3 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-mROCK1-delta3

Plasmid

#101291

Purpose

Mammalian expression of Rock1-GFP for the study of localisation signaling.

Depositor

Alpha Yap

Article

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Insert

Rock1 (Rock1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

1-1080 aa

Promoter

CMV

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pIRES RED/SP M1C448Y

Plasmid

#89323

Purpose

Expression of RFP and M1C448Y spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

Insert

spastin (SPAST Human)

Use

Tags

Expression

Mammalian

Mutation

1-194 bp of 5'UTR deleted

Promoter

CMV

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pOPTN217-577-EGFP

Plasmid

#68841

Purpose

deleted OPTN was inserted into pEGFP-N1 backbone

Depositor

Beatrice Yue

Article

Turturro et al Springerplus. 2014 Feb 19;3:99. doi: 10.

Insert

optineurin (OPTN Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

aa 210-577 only

Promoter

CMV

Available since

March 9, 2016

Availability

Academic Institutions and Nonprofits only
pOPTN1-209-EGFP

Plasmid

#68839

Purpose

deleted OPTN was inserted into pEGFP-N1 backbon

Depositor

Beatrice Yue

Article

Turturro et al Springerplus. 2014 Feb 19;3:99. doi: 10.

Insert

optineurin (OPTN Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

aa 1-209 only

Promoter

CMV

Available since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only
FAM19A2-V5

Plasmid

#184338

Purpose

Expression of V5-tagged mouse FAM19A2

Depositor

Peter Scheiffele

Article

Hauser et al Neuron. 2022 May 8. pii: S0896-6273(22)0

Insert

FAM19A2 (Tafa2 Mouse)

Use

Tags

V5-tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-M2-TLR2

Plasmid

#12291

Depositor

Bruce Beutler

Article

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

TLR2 (Tlr2 Mouse)

Use

Tags

FLAG (FLAG-M2)

Expression

Mammalian

Mutation

M82I and H202Y; signal peptide (aa1-23) replaced …

Promoter

Available since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only
pQ398X-EGFP

Plasmid

#68849

Purpose

mutated OPTN was inserted into pEGFP-N1 backbone

Depositor

Beatrice Yue

Article

Turturro et al Springerplus. 2014 Feb 19;3:99. doi: 10.

Insert

optineurin (OPTN Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Q398X, results in truncation of the protein into …

Promoter

CMV

Available since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only
pSMP-Suv39H1_1

Plasmid

#36342

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

Suv39H1 (SUV39H1 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
HA-TRIM28deltaCC2

Plasmid

#124957

Purpose

Mammalian expression of Human TRIM28 with deleted CC domain

Depositor

Michelle Barton

Article

Li et al Oncogene. 2017 May 25;36(21):2991-3001.

Insert

hTRIM28 (TRIM28 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Deletion of CC domains

Promoter

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pECFP-Cdc42Q61L

Plasmid

#105294

Purpose

Constitutively active Cdc42

Depositor

Kenneth Yamada

Article

Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

Insert

Cdc42 (CDC42 Human)

Use

Tags

CFP

Expression

Mammalian

Mutation

Q61L

Promoter

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-TRIM28deltaCC1

Plasmid

#124955

Purpose

Mammalian expression of Human TRIM28 with deleted CC domain

Depositor

Michelle Barton

Article

Li et al Oncogene. 2017 May 25;36(21):2991-3001.

Insert

hTRIM28 (TRIM28 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Deletion of CC domains

Promoter

Available since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
CAG:: ChR2HA-2a-hM4D

Plasmid

#52520

Purpose

Co-expresses HA tagged channelrhodopsin and hM4D (Gi)

Depositor

Scott Sternson

Article

Stachniak et al Neuron. 2014 May 21;82(4):797-808. doi:

Insert

ChR2-2a-hM4D

Use

Tags

2xHA on ChR2

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 9, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
mEos2-Golgi-7

Plasmid

#57382

Purpose

Localization: Golgi Complex, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Golgi (B4GALT1 Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

aa 1-82 of NM_001497.3

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pOPTN425-577-EGFP

Plasmid

#68843

Purpose

deleted OPTN was inserted into pEGFP-N1 backbone

Depositor

Beatrice Yue

Article

Turturro et al Springerplus. 2014 Feb 19;3:99. doi: 10.

Insert

optineurin (OPTN Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

aa 425-577 only

Promoter

CMV

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only
pOPTN1-424-EGFP

Plasmid

#68840

Purpose

deleted OPTN was inserted into pEGFP-N1 backbone

Depositor

Beatrice Yue

Article

Turturro et al Springerplus. 2014 Feb 19;3:99. doi: 10.

Insert

optineurin (OPTN Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

aa 1-424 only

Promoter

CMV

Available since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only
pCMV-Tag/WT M87

Plasmid

#89322

Purpose

expression of WT M87 spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

Insert

spastin (SPAST Human)

Use

Tags

Expression

Mammalian

Mutation

1-460 bp deleted

Promoter

CMV

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-Tag 3A/WT myc-M1

Plasmid

#87719

Purpose

Expresses WT myc-M1 spastin isoform in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

M1 isoform

Promoter

CMV

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pL157A-EGFP

Plasmid

#68850

Purpose

mutated OPTN was inserted into pEGFP-N1 backbone

Depositor

Beatrice Yue

Article

Turturro et al Springerplus. 2014 Feb 19;3:99. doi: 10.

Insert

optineurin (OPTN Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

L157A, mutation has not been linked to any diseas…

Promoter

CMV

Available since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only
pSMP-Suv39H1_2

Plasmid

#36343

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

Suv39H1 (SUV39H1 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pYPQ-dpcoCas9-EV2.1

Plasmid

#158411

Purpose

CRISPR-dpcoCas9 fused with EDLL activator for transcriptional activation

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Insert

dpcoCas9-EDLL-T2A-MS2-VPR(VP64-p65-Rta)-NOS-ter

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-Tag/WT M1

Plasmid

#89321

Purpose

expression of M1 C448Y spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

Insert

spastin (SPAST Human)

Use

Tags

Expression

Mammalian

Mutation

1-194 bp of 5'UTR deleted

Promoter

CMV

Available since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge

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