Addgene

We narrowed to 15,391 results for: SHE

Showing: 1651 - 1700 of 15391 results

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integrin beta6 pcDNA1 neo

Plasmid

#13580

Depositor

Dean Sheppard

Article

Weinacker et al J Biol Chem. 1994 Mar 4. 269(9):6940-8.

Insert

integrin beta 6 (ITGB6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 8, 2007

Availability

Academic Institutions and Nonprofits only

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pHMT.wt (p53)

Plasmid

#12139

Depositor

James Sherley

Article

Liu et al J Cell Physiol. 1998 Nov . 177(2):364-76

Insert

p53 (Trp53 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2006

Availability

Academic Institutions and Nonprofits only
HsCLYBL-FLAG

Plasmid

#111290

Purpose

mammalian expression

Depositor

Vamsi Mootha

Article

Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

Insert

human CLYBL (CLYBL Human)

Use

Tags

linker and FLAG tag: TGGGSGGSGGSMDYKDDDDK

Expression

Mammalian

Mutation

Promoter

Available since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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HsCLYBL(D320A)-FLAG

Plasmid

#111291

Purpose

mammalian expression

Depositor

Vamsi Mootha

Article

Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

Insert

human CLYBL D320A (CLYBL Human)

Use

Tags

linker and FLAG tag: TGGGSGGSGGSMDYKDDDDK

Expression

Mammalian

Mutation

D320A

Promoter

Available since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pNH11 (pmyo-2::Arch(D95N)::2xMycTag)

Plasmid

#130275

Purpose

Expresses the voltage sensor Arch(D95N) in the pharynx of C. elegans.

Depositor

Alexander Gottschalk

Article

Azimi Hashemi et al Proc Natl Acad Sci U S A. 2019 Aug 1. pi

Insert

Arch(D95N)

Use

Tags

2x Myc-tag

Expression

Worm

Mutation

Promoter

Available since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pBluescript GPX-1

Plasmid

#13038

Depositor

Peter Newburger

Article

Shen et al J Biol Chem. 1993 May 25. 268(15):11463-

Insert

GPX-1 (GPX1 Human)

Use

Tags

Expression

Bacterial

Mutation

Additional GCG upstream of codon 11. Codon 92 is …

Promoter

Available since

Nov. 7, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3 MDM2 1-400

Plasmid

#45754

Depositor

Robert Lefkowitz

Article

Shenoy et al Science. 2001 Nov 9;294(5545):1307-13. E

Insert

MDM2 1-400 (MDM2 Human)

Use

Tags

Expression

Mammalian

Mutation

aa 1-400, lacks catalytic RING domain

Promoter

CMV

Available since

Aug. 21, 2013

Availability

Academic Institutions and Nonprofits only
pNH13 (pmyo-2::QuasAr::mOrange)

Plasmid

#130273

Purpose

Expresses the eFRET-based voltage sensor QuasAr::mOrange in the pharynx of C. elegans.

Depositor

Alexander Gottschalk

Article

Azimi Hashemi et al Proc Natl Acad Sci U S A. 2019 Aug 1. pi

Insert

QuasAr::mOrange

Use

Tags

mOrange

Expression

Worm

Mutation

Promoter

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CMV-SspB-EGFP-VP16-P2A-FLAG-Gal4DBD-CapN-SsrA-CapC-HA

Plasmid

#213536

Purpose

Expresses the split transcription factors in mouse liver

Depositor

Wenjing Wang

Article

Shen et al Nat Methods. 2023 Jan;20(1):112-122. doi

Insert

SspB-EGFP-VP16-P2A-FLAG-Gal4DBD-CapN-SsrA-CapC-HA-HA

Use

AAV

Tags

EGFP and HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
HOXB8 (murine) HIS-tag pET

Plasmid

#8540

Depositor

Corey Largman

Article

Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

Insert

HOXB8 (Hoxb8 Mouse)

Use

Tags

His and T7

Expression

Bacterial

Mutation

starts at amino acid #2 (Ser)

Promoter

Available since

Aug. 7, 2006

Availability

Academic Institutions and Nonprofits only
trLATmEos3.2

Plasmid

#203745

Purpose

Encodes the transmembrane domain from human LAT with mEos3.2 fused on the C terminus. Used as a fluorescent plasma membrane probe.

Depositor

Sarah Veatch

Article

Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

Insert

trLAT (LAT Human)

Use

Tags

mEos3.2

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLdCH

Plasmid

#84291

Purpose

Express gRNA and Cas9 in Leishmania with Hygromycin resistance

Depositor

Greg Matlashewski

Article

Zhang et al mSphere. 2017 Jan 18;2(1). pii: e00340-1

Insert

gRNA and Cas9

Use

CRISPR; Leishmania

Tags

Expression

Mutation

Promoter

L. donovani ribosome RNA promoter

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
HOXA7 (murine) His-tag pET

Plasmid

#8491

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

Insert

HOXA7 (Hoxa7 Mouse)

Use

Tags

His and T7

Expression

Bacterial

Mutation

Fusion sequence is: ggatccATG; makes protein by …

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
HOXB4 (human) pSG5

Plasmid

#8527

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB4 (HOXB4 Human)

Use

In vitro transcriptioin/translation

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pGEX-6P-1-hs-ALPS 1-ALPS2-mCherry

Plasmid

#187114

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSG00000101199 (ARFGAP1 Human)

Use

Tags

mCherry

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
HOXA4 (murine) HA-tag pET

Plasmid

#8489

Depositor

Corey Largman

Article

Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

Insert

HOXA4 (Hoxa4 Mouse)

Use

Tags

HA and T7

Expression

Bacterial

Mutation

Thr-2 changed to Ala-2 to form better ribosomal b…

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
M77 pIRESpuro-GLUE-hDsh3

Plasmid

#15102

Depositor

Randall Moon

Article

Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

Insert

Dsh3 (DVL3 Human)

Use

Tags

CBP tag, HA, and streptag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
XE124 XDsh delta DEP-GFP-CS2+

Plasmid

#16785

Depositor

Randall Moon

Article

Miller et al J Cell Biol. 1999 Jul 26;146(2):427-37.

Insert

Xdsh-delta-DEP (dvl2.L Frog)

Use

Xenopus expression

Tags

GFP

Expression

Mutation

contains Dsh amino acids 1-433 and 503-736 (delet…

Promoter

Available since

March 14, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
p53-(1-393)-R273H-N268D

Plasmid

#35087

Depositor

Cheryl Arrowsmith

Article

Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

Insert

p53 (TP53 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Contains aa 1-393 - R273H-N268D

Promoter

T7

Available since

April 4, 2012

Availability

Academic Institutions and Nonprofits only
p164-pAAV-mDlx-FLEX-mScarlet

Plasmid

#204689

Purpose

Fluorescent reporter under the control of the Dlx promotor

Depositor

Lynette Lim

Article

Fisher et al Neuron. 2023 Dec 7:S0896-6273(23)00887-5

Insert

mScarlet

Use

AAV and Cre/Lox

Tags

mScarlet palmitoylation

Expression

Mammalian

Mutation

Promoter

mDlx

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
p164 Dync1i1.B

Plasmid

#26435

Depositor

Elizabeth Fisher

Article

Kuta et al PLoS One. 2010 . 5(7):e11682.

Insert

cytoplasmic dynein 1 intermediate chain 1 isoform B (Dync1i1 Mouse)

Use

Subcloning and sequencing

Tags

Expression

Mutation

N/A

Promoter

Available since

March 21, 2011

Availability

Academic Institutions and Nonprofits only
pNH12 (pmyo-2::MacQ::mCitrine)

Plasmid

#130274

Purpose

Expresses the eFRET-based voltage sensor MacQ::mCitrine in the pharynx of C. elegans.

Depositor

Alexander Gottschalk

Article

Azimi Hashemi et al Proc Natl Acad Sci U S A. 2019 Aug 1. pi

Insert

MacQ::mCitrine

Use

Tags

mCitrine

Expression

Worm

Mutation

Promoter

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBtPIPLC His

Plasmid

#173808

Purpose

Expression and purification of the mature form of Bacillus thuringiensis phosphaatidyl inositol specific phospholipase C (PI-PLC), UniProt ID P08954 from E. coli BL21 CodonPlus (DE3) RIL cells

Depositor

Anne Gershenson, Mary F Roberts

Article

He et al J Biol Chem. 2015 Jul 31;290(31):19334-4

Insert

Bacillus thuringiensis 1-phosphatidylinositol phosphodiesterase

Use

Tags

6XHis

Expression

Bacterial

Mutation

none

Promoter

T7

Available since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
p53-(1-393)-R175H-N268D

Plasmid

#35086

Depositor

Cheryl Arrowsmith

Article

Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

Insert

p53 (TP53 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Contains aa 1-393 - R175H-N268D

Promoter

T7

Available since

April 4, 2012

Availability

Academic Institutions and Nonprofits only
pMSCV-Atoh1wt-IRES-RFP

Plasmid

#33334

Depositor

Charles Sherr, Martine Roussel

Article

Ayrault et al Cancer Res. 2010 Jul 1;70(13):5618-27. E

Insert

Atoh1 (Atoh1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 31, 2012

Availability

Academic Institutions and Nonprofits only
HOXA11 (murine) HIS-tag pET

Plasmid

#8517

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

Insert

HOXA11 (Hoxa11 Mouse)

Use

Tags

T7

Expression

Bacterial

Mutation

makes protein by Western

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
p199 Dync1i2.C (Ex1a)

Plasmid

#26449

Depositor

Elizabeth Fisher

Article

Kuta et al PLoS One. 2010 . 5(7):e11682.

Insert

cytoplasmic dynein 1 intermediate chain 2 isoform C (exon 1a) (Dync1i2 Mouse)

Use

Subcloning and sequencing

Tags

Expression

Mutation

N/A

Promoter

Available since

March 24, 2011

Availability

Academic Institutions and Nonprofits only
pET30a-SARS-CoV2-ORF8

Plasmid

#165089

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Article

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Severe acute respiratory syndrome coronavirus 2 ORF8 (ORF8 SARS-CoV2)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
HOXB2 (human) HIS-tag pET

Plasmid

#8522

Depositor

Corey Largman

Article

Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

Insert

HOXB2 (HOXB2 Human)

Use

Tags

His and T7

Expression

Bacterial

Mutation

Starts at AA #2; makes protein by Western

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pLNCX2-Orai1

Plasmid

#89818

Purpose

Express Orai1 in mammalian cells

Depositor

Shengyu Yang

Article

Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

Insert

Orai1 (ORAI1 Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
HBT-CPK32-Flag

Plasmid

#107432

Purpose

For Arabidopsis mesophyll protoplast transient expression assay

Depositor

Jen Sheen

Article

Liu et al Nature. 2017 May 18;545(7654):311-316. d

Insert

CPK32 (CPK32 Mustard Weed)

Use

Tags

Flag

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
HSF1 KO

Plasmid

#200207

Purpose

gRNA for HSF1 KO

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

HSF1 KO (Hsf1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

None

Promoter

U6

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
XW17

Plasmid

#65835

Purpose

Punc-4-QF-3'UTR-SL2-mCherry-unc-54 (The endogenous SphI, KpnI and SacI sites in QF are removed for the convenience of cloning, most sites are compatible with pPD95.77)

Depositor

Kang Shen

Article

Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

Insert

OR74A quinic acid utilization activator (qa-1F)

Use

Tags

mCherry

Expression

Worm

Mutation

The endogenous SphI, KpnI and SacI sites in QF ar…

Promoter

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
p53-(1-393)-E285K-N268D

Plasmid

#35089

Depositor

Cheryl Arrowsmith

Article

Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

Insert

p53 (TP53 Human)

Use

Tags

His and Thrombin cleavage site

Expression

Bacterial

Mutation

Contains aa 1-393 with N269D and E285K mutations

Promoter

T7

Available since

April 4, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
XE123 XDsh delta PDZ-GFP- CS2+

Plasmid

#16786

Depositor

Randall Moon

Article

Miller et al J Cell Biol. 1999 Jul 26;146(2):427-37.

Insert

Xdsh-delta-PDZ (dvl2.L Frog)

Use

Xenopus expression

Tags

gfp

Expression

Mutation

contains Dsh amino acids 1-267 and 381-736 (delet…

Promoter

Available since

March 14, 2008

Availability

Academic Institutions and Nonprofits only
pMSCV-Atoh1wt-IRES-GFP

Plasmid

#33333

Depositor

Charles Sherr, Martine Roussel

Article

Ayrault et al Cancer Res. 2010 Jul 1;70(13):5618-27. E

Insert

Atoh1 (Atoh1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 31, 2012

Availability

Academic Institutions and Nonprofits only
pFastBac HT JS-Munc18b

Plasmid

#135554

Purpose

Express rat Munc18b in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18b.

Depositor

Jingshi Shen

Article

Yu et al Methods Mol Biol. 2019;1860:237-249. doi

Insert

Munc18b (Stxbp2 Rat)

Use

Tags

6xHis tag and TEV site

Expression

Insect

Mutation

Promoter

polyhedrin promoter

Available since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSUPER.retro.puro-shMePCE_shLARP7

Plasmid

#113538

Purpose

Retroviral vector designed to knock down MePCE and LARP7 simultaneously.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

shMePCE and shLARP7 (MEPCE Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-Atoh1ER-IRES-GFP

Plasmid

#33335

Depositor

Charles Sherr, Martine Roussel

Article

Ayrault et al Cancer Res. 2010 Jul 1;70(13):5618-27. E

Insert

Atoh1 (Atoh1 Mouse)

Use

Retroviral

Tags

ER

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 31, 2012

Availability

Academic Institutions and Nonprofits only
HOXB7 (human) HIS-tag pET

Plasmid

#8538

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB7 (HOXB7 Human)

Use

Tags

His and T7

Expression

Bacterial

Mutation

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
HOXA9 (murine) FLAG pRC/CMV

Plasmid

#8514

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

Insert

HOXA9 (Hoxa9 Mouse)

Use

Tags

FLAG and His

Expression

Mammalian

Mutation

FLAG epitope tag replaces T7 tag in the pET back…

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
CBP-Bromo-HAT-CH3-sp65

Plasmid

#21026

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 2001 Nov;21(21):7509-22

Insert

CBP (Crebbp Mouse)

Use

In vitro tnt

Tags

Flag

Expression

Mutation

Bromo+HAT+CH3 domains aa 1098-1877; E1239G mutati…

Promoter

Available since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pSPneogRNA241510+MT

Plasmid

#84292

Purpose

Express LdPBK_241510.1 and LdMT targeting gRNAs simutaneously

Depositor

Greg Matlashewski

Article

Zhang et al mSphere. 2017 Jan 18;2(1). pii: e00340-1

Insert

LdBPK_241510.1 targeting gRNA and LdMT targeting gRNA

Use

CRISPR; Leishmania donovani

Tags

Expression

Mutation

Promoter

L. donovani ribosome RNA promoter

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pFlg167

Plasmid

#101019

Purpose

fluorescent, functional, glutamate receptor subunit

Depositor

Thomas Hughes

Article

Sheridan et al Neuroscience. 2006 Aug 25;141(2):837-49.

Insert

GluR 1 (Gria1 Rat)

Use

Tags

Expression

Mammalian

Mutation

fluorescent protein inserted into subunit

Promoter

cmv

Available since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO-shcIAP1-B

Plasmid

#44131

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

c-IAP1 (BIRC2 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 10, 2013

Availability

Academic Institutions and Nonprofits only
FusionRed-Sit-N-15

Plasmid

#56133

Purpose

Localization: Golgi TGN, Excitation: 580, Emission: 608

Depositor

Michael Davidson

Article

Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

Insert

Sit (ST6GAL1 Human)

Use

Tags

FusionRed

Expression

Mammalian

Mutation

aa 1-45 of SiT

Promoter

CMV

Available since

April 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits
HOXC4 (human) MSCV

Plasmid

#8546

Depositor

Corey Largman

Article

Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

Insert

HOXC4 (HOXC4 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Contains improved ribosomal binding site and NcoI…

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pFlg113

Plasmid

#101020

Purpose

fluorescent, functional, glutamate receptor subunit

Depositor

Thomas Hughes

Article

Sheridan et al Neuroscience. 2006 Aug 25;141(2):837-49.

Insert

GluR 1 (Gria1 Rat)

Use

Tags

Expression

Mammalian

Mutation

fluorescent protein inserted into subunit

Promoter

cmv

Available since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
HOXD12 (murine) pRSETa

Plasmid

#8554

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

Insert

HOXD12 (Hoxd12 Mouse)

Use

Tags

HIS and XPress

Expression

Bacterial

Mutation

Fusion sequence is: ggatccATG

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pMSCV FHA-Stop

Plasmid

#27050

Depositor

Jayanta Debnath

Article

Radoshevich et al Cell. 2010 Aug 20. 142(4):590-600.

Insert

autophagy-related 12 (yeast) (Atg12 Mouse)

Use

Retroviral

Tags

HA and flag

Expression

Mammalian

Mutation

changed glycine 141 to stop codon.

Promoter

Available since

Jan. 13, 2011

Availability

Academic Institutions and Nonprofits only

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