161,398 results

1. pAAV-FLEXfrt-SaCas9-U6-sgSlc18a2

   Plasmid

   #124862

   Purpose

   Mutagenesis of Slc18a2

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Slc18a2 (Slc18a2 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. TDP-REGv2(mScarlet-FLAG)#7 in pTwist-CMV backbone

   Plasmid

   #216152

   Purpose

   Expresses mScarlet in cells with TDP-43 loss-of-function. This plasmid has one of the best dynamic ranges of those tested (~100-fold) in SK-N-BE2 cells. It uses a cryptic exon. [Code 'A11'] Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

   Depositor

   Pietro Fratta

   Article

   Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

   Insert

   mScarlet with cryptic exon and C-terminal FLAG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA5/FRT_CMV_NES-Caprola_off-mEGFP

   Plasmid

   #194687

   Purpose

   CMV driven expression of the inactive calcium recorder negative control Caprola_off in mammalian cell lines fused to mEGFP

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_off-mEGFP
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Yeast Inducible CRISPRi Library

   Pooled Library

   #161829

   Purpose

   ATc inducbile yeast CRISPRi library

   Depositor

   Saeed Tavazoie

   Article

   Momen-Roknabadi et al Commun Biol. 2020 Nov 27;3(1):723. doi:

   Expression

   Yeast

   Species

   Saccharomyces cerevisiae

   Use

   CRISPR

   Available since

   April 28, 2021

   Availability

   Academic Institutions and Nonprofits only

5. pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W

   Plasmid

   #67974

   Purpose

   CRISPR gRNA expression vector with an improved scaffold and puro/BFP markers

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette, PGKpuro2ABFP cassette, WPRE
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Deleted BbsI site within WPRE

   Promoter

   human U6 promoter, mouse PGK promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. GUT BuGZ FL

   Plasmid

   #84024

   Purpose

   Expression of GFP tagged BuGZ in human cells

   Depositor

   Patrick Paddison

   Article

   Toledo et al Dev Cell. 2014 Feb 10;28(3):282-94. doi:

   Insert

   BuGZ (ZNF207 Human)
   

   Use

   Lentiviral

   Tags

   TurboGFP

   Expression

   Mammalian

   Mutation

   Q394R

   Promoter

   UCOE / SFFV

   Available since

   Oct. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGH125_dCas9-Blast

   Plasmid

   #85417

   Purpose

   lentiviral expression vector for dCas9 with Blasticidin selectable marker

   Depositor

   Michael Bassik

   Article

   Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   dCas9 and Blasticidin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA GNSTM-3-Flag-10-Lamp2b-HA

   Plasmid

   #71293

   Purpose

   Encodes (N to C): GNSTM glycosylation motif, 3 residue spacer, Flag tag,10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.

   Depositor

   Joshua Leonard

   Article

   Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72

   Insert

   Lamp2b (LAMP2 Human)
   

   Use

   Tags

   FLAG, GNSTM glycosylation motif, HA, and Lamp2 si…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSDM052

   Plasmid

   #194341

   Purpose

   pUC mini-Tn7 vector expressing LseCas13a

   Depositor

   Joseph Bondy-Denomy

   Article

   Mendoza et al Nature. 2020 Jan;577(7789):244-248. doi:

   Insert

   Cas13a(Lse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. EB3N-VVDfast-Halo-iLID

    Plasmid

    #190166

    Purpose

    Expresses EB3N-VVDfast-Halo-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells.

    Depositor

    Anna Akhmanova

    Article

    Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

    Insert

    EB3N-VVDfast-Halo-iLID
    

    Use

    Tags

    -VVDfast-mClover3-iLID

    Expression

    Mammalian

    Mutation

    1-189 aa from EB3

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGreenFire1_E2F1RE

    Plasmid

    #112248

    Purpose

    expression of GFP and Luciferase under the control of E2F1 responsive element

    Depositor

    Simone Di Giovanni

    Insert

    E2F1RE (responsive element)
    

    Use

    Lentiviral

    Tags

    copGFP and luciferase

    Expression

    Mutation

    Promoter

    Available since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSYFP2-SCFP3A

    Plasmid

    #22905

    Depositor

    Dorus Gadella

    Article

    Kremers et al Biochemistry. 2006 May 30. 45(21):6570-8

    Insert

    SYFP2-SCFP3A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLPC-scribP305L

    Plasmid

    #24601

    Depositor

    Senthil Muthuswamy

    Article

    Zhan et al Cell. 2008 Nov 28. 135(5):865-78.

    Insert

    Scribble (SCRIB Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    P305L

    Promoter

    Available since

    May 5, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pLV hUbC-dCas9 KRAB-T2A-GFP

    Plasmid

    #67620

    Purpose

    Co-expressed human optimized S. pyogenese dCas9 fused to KRAB repressor domain and GFP

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Insert

    humanized dead Cas9 KRAB T2A GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mutation

    D10A and H840A

    Promoter

    Available since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. L304-EGFP-Tubulin-WT

    Plasmid

    #64060

    Purpose

    EGFP fused at the N-terminus of tubulin; lentiviral construct

    Depositor

    Weiping Han

    Article

    Yang et al Biochem J. 2013 Feb 1;449(3):605-12. doi

    Insert

    tubulin
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMMP-MCS-IRES-eGFP-P2A-iCas9

    Plasmid

    #178533

    Purpose

    For genome editing: EGFP-inducible Caspase9 knock-in cassette which allows ablation of target cells

    Depositor

    Ryoji Yao

    Article

    Okamoto et al STAR Protoc. 2021 Sep 16;2(4):100780. do

    Insert

    iCaspase9 (CASP9 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    FKBP12 F36 to V Truncated Caspase9 (135-416; lack…

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-hSyn-PROGERIN

    Plasmid

    #140189

    Purpose

    Overexpression of Progerin with a GFP fusion protein, under human synapsin promotor

    Depositor

    Lorenz Studer

    Article

    Miller et al Cell Stem Cell. 2013 Dec 5;13(6):691-705

    Insert

    Progerin (LMNA Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-Flpe:GFP

    Plasmid

    #13788

    Purpose

    Mammalian expression of enhanced form of Flp recombinase fused to GFP

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    Flpe-GFP fusion protein
    

    Use

    Flp/frt

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. pORANGE GFP-Actb KI

    Plasmid

    #131479

    Purpose

    Endogenous tagging of βactin: N-terminal (amino acid position: D2)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Actb, Actb Rat, Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV5-ActRIIB

    Plasmid

    #13501

    Depositor

    Jeff Wrana

    Article

    Macias-Silva et al J Biol Chem. 1998 Oct 2. 273(40):25628-3

    Insert

    Activin receptor IIB (Acvr2b Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 4, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. pEGFP-C1-TFIIB

    Plasmid

    #26675

    Depositor

    Sui Huang

    Article

    Chen et al Mol Biol Cell. 2002 Jan . 13(1):276-84.

    Insert

    TFIIB (GTF2B Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2010

    Availability

    Academic Institutions and Nonprofits only

22. PB CAG-sfGFP

    Plasmid

    #133568

    Purpose

    CAG sfGFP piggyBac transposon

    Depositor

    Mario Capecchi

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. PCR4-Hsp68::mCherry-SI-Hsp68::eGFP-H11

    Plasmid

    #211942

    Purpose

    dual-enSERT-2.2 vector for site-specific integration of.a two-color enhancer-reporter construct into the H11 locus (separated by a synthetic insulator)

    Depositor

    Evgeny Kvon

    Article

    Hollingsworth et al bioRxiv 2023.12.10.570890

    Type

    Empty backbone

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    HSP68

    Available since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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24. pENN.AAV.hSyn.Cre.WPRE.hGH (AAV8)

    Viral Prep

    #105553-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pENN.AAV.hSyn.Cre.WPRE.hGH (#105553). In addition to the viral particles, you will also receive purified pENN.AAV.hSyn.Cre.WPRE.hGH plasmid DNA. hSyn-driven Cre expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    James M. Wilson

    Promoter

    Syn

    Tags

    None

    Available since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

25. pcDNA3-6XHis-human SUMO 2

    Plasmid

    #133771

    Purpose

    express 6Xhis- SUMO 2 protein

    Depositor

    Martine Roussel

    Article

    Tago et al Proc Natl Acad Sci U S A. 2005 May 24. 1

    Insert

    SUMO 2 (SUMO2 Human)
    

    Use

    Tags

    6XHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. His-GFP-Syntaxin1A

    Plasmid

    #170871

    Purpose

    Mammalian expression of His-GFP-Syntaxin1A.

    Depositor

    Justin Taraska

    Article

    Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

    Insert

    Syntaxin 1A (Stx1a Mouse)
    

    Use

    Tags

    (His)6 and EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEGFP-BLM

    Plasmid

    #110299

    Purpose

    Mammalian expression of a EGFP-tagged full length Bloom's syndrome helicase

    Depositor

    Chris Kok-Lung Chan

    Insert

    BLM (Bloom's Syndrome) helicase (BLM Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. GPR82-Tango

    Plasmid

    #66375

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPR82 (GPR82 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

29. pFH2.105_PB-EPN24-MCP-T2A-eGFP-PGK-Blast

    Plasmid

    #205550

    Purpose

    Express EPN24-MCP-T2A-eGFP and blasticidin resistance marker

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    EPN24-MCP
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. AAV CMV-dSaCas9-KRAB-bGHpA

    Plasmid

    #106219

    Purpose

    AAV expressing deactivated S. aureus Cas9 fused to a KRAB repressor

    Depositor

    Charles Gersbach

    Article

    Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

    Insert

    dead S. aureus Cas9 KRAB
    

    Use

    AAV and CRISPR

    Tags

    HA-NLS

    Expression

    Mammalian

    Mutation

    D10A and N580A

    Promoter

    CMV

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge