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Plasmid#113610PurposePPI reporterDepositorInsertbeta 2 adrenergic receptor
ExpressionMammalianAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSYN-bArrestin2-TevC-P2A-TdTomato-WPRE-bGHpA
Plasmid#89873Purposeexpresses iTango bArrestin2-TevC component and TdTomato bicistronically for AAV productionDepositorInsertbArrestin2-TevC-P2A-TdTomato
UseAAVTagsTdTomatoExpressionMammalianMutationR173H in TdTomato (Please see depositor comments …PromoterHuman synapsinAvailable sinceMay 26, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PCDNA3-PAR1-GFP11-T2A-GFP10-bArrestin1-T2A mCherry
Plasmid#113608PurposePPI reporterDepositorInsertPAR1
ExpressionMammalianAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET C-terminal TEV His6 cloning vector with BioBrick polycistronic restriction sites (9Bc)
Plasmid#48285DepositorTypeEmpty backboneTagsHis6ExpressionBacterialAvailable sinceNov. 5, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
psPAX2
Plasmid#12260Purpose2nd generation lentiviral packaging plasmid. Can be used with 2nd or 3rd generation lentiviral vectors and envelope expressing plasmid (Addgene#12259)DepositorTypeEmpty backboneUseLentiviral; PackagingExpressionMammalianAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA (AAV1)
Viral prep#50942-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA (#50942). In addition to the viral particles, you will also receive purified pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA plasmid DNA. GCaMP6s calcium sensor and bicistronic, physically separate mRuby2 expression under a human synapsin1 promoter. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmRuby2Available sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMD2.G
Plasmid#12259PurposeVSV-G envelope expressing plasmidDepositorInsertVSV G
UseLentiviral; EnvelopeExpressionMammalianAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFastBac StrepII msfGFP TEV cloning vector with BioBrick polycistronic restriction sites(11R-GFP)
Plasmid#48297DepositorTypeEmpty backboneTagsStrepII and msfGFPExpressionInsectAvailable sinceSept. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2
Plasmid#52961PurposeReplaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.DepositorInsertsCas9
Puromycin resistance
UseCRISPR and LentiviralTagsFLAGExpressionMammalianPromoterEFS-NSAvailable sinceMay 8, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
psPAX2
Plasmid#12260Purpose2nd generation lentiviral packaging plasmid. Can be used with 2nd or 3rd generation lentiviral vectors and envelope expressing plasmid (Addgene#12259)DepositorTypeEmpty backboneUseLentiviral; PackagingExpressionMammalianAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSpCas9(BB)-2A-GFP (PX458)
Plasmid#48138PurposeCas9 from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNADepositorInserthSpCas9
UseCRISPRTags3XFLAG and GFPExpressionMammalianPromoterCbhAvailable sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMD2.G
Plasmid#12259PurposeVSV-G envelope expressing plasmidDepositorInsertVSV G
UseLentiviral; EnvelopeExpressionMammalianAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSpCas9(BB)-2A-Puro (PX459) V2.0
Plasmid#62988PurposeCas9 from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)DepositorInserthSpCas9-2A-Puro V2.0
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianPromoterCbhAvailable sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRSV-Rev
Plasmid#12253Purpose3rd generation lentiviral packaging plasmid; Contains Rev; also requires pMDLg/pRRE (Addgene#12251) and envelope expressing plasmid (Addgene#12259)DepositorPublicationAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMDLg/pRRE
Plasmid#12251Purpose3rd generation lentiviral packaging plasmid; Contains Gag and Pol; also requires pRSV-Rev (Addgene#12253) and envelope expressing plasmid (Addgene#12259)DepositorPublicationInsertHIV-1 GAG/POL
UseLentiviral; PackagingExpressionMammalianAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-VSV-G
Plasmid#8454PurposeEnvelope protein for producing lentiviral and MuLV retroviral particles. Use in conjunction with a packaging vector such as pCMV-dR8.2 dvpr (lentiviral) or pUMVC (MuLV retroviral).DepositorPublicationTypeEmpty backboneExpressionMammalianAvailable sinceJune 20, 2005AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX330-U6-Chimeric_BB-CBh-hSpCas9
Plasmid#42230PurposeA human codon-optimized SpCas9 and chimeric guide RNA expression plasmid.DepositorPublicationInserthumanized S. pyogenes Cas9
UseCRISPRTags3xFLAGExpressionMammalianPromoterCBhAvailable sinceFeb. 27, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
lentiGuide-Puro
Plasmid#52963PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.DepositorInsertsS. pyogenes sgRNA cassette
Puromycin resistance
UseCRISPR and LentiviralExpressionMammalianPromoterEf1-a and hU6Available sinceMay 8, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Tet-pLKO-puro
Plasmid#21915Purpose3rd generation lentiviral plasmid for inducible expression of shRNA; puromycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.DepositorTypeEmpty backboneUseLentiviralAvailable sinceAug. 22, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLKO.1 - TRC cloning vector
Plasmid#10878Purpose3rd generation transfer plasmidDepositorPublicationTypeEmpty backboneUseLentiviral and RNAiExpressionMammalianAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by