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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 25,285 results for: crispr

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Showing: 801 - 820 of 25285 results

  1. Tet-inducible Luciferase reporter

    Plasmid
    #64127
    Purpose
    Photoactivatable transcription system. Luciferase reporter under the control of a tet-inducible promoter.
    Depositor
    Moritoshi Sato
    Publication
    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    luciferase
    Use
    Luciferase
    Expression
    Mammalian
    Promoter
    TetOx13-CMV
    Available since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    64127-plasmid-map-sequence-id-198432

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  2. pPP441

    Plasmid
    #158801
    Purpose
    Plasmid containing Homo sapiens codon optimized CasΦ-2 and a SapI-GG stuffer spacer for editing in human cells.
    Depositor
    Jennifer Doudna
    Publication
    Pausch et al Science. 2020 Jul 17;369(6501):333-337.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CasPhi-2
    Tags
    2xSV40NLS-2xFLAG
    Expression
    Mammalian
    Available since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    158801-plasmid-map-sequence-id-309574

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  10. pLentiRNAGuide_001 - hU6-RfxCas13d-DR1-BsmBI-EFS-Puro-WPRE

    Plasmid
    #138150
    Purpose
    For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa
    Depositor
    Neville Sanjana
    Publication
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    hU6_Rfxcas13d-DR1_BsmBI
    Use
    Lentiviral
    Mutation
    DR1 A>T
    Available since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    16 Articles
    138150-plasmid-map-sequence-id-350242

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  16. pCMV-dCas13-M3nls

    Plasmid
    #155366
    Purpose
    Targeted m6A RNA methylation in mammalian cells
    Depositor
    David Liu
    Publication
    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas13-M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    PspCas13b Δ984-1090 H133A
    Available since
    July 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    7 Articles
    155366-plasmid-map-sequence-id-305808

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  18. GAL4UAS-Luciferase reporter

    Plasmid
    #64125
    Purpose
    Photoactivatable transcription system. Luciferase reporter under the control of the upstream activator sequence (UAS) of Gal4.
    Depositor
    Moritoshi Sato
    Publication
    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GAL4UAS
    Use
    Luciferase
    Tags
    luciferase
    Expression
    Mammalian
    Promoter
    GAL4UAS
    Available since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    10 Articles
    64125-plasmid-map-sequence-id-248531

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  20. pCMV-PEmax-P2A-BSD

    Plasmid
    #174821
    Purpose
    Mammalian expression of SpCas9 PEmax prime editor with P2A-BSD marker
    Depositor
    David Liu
    Publication
    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PEmax-P2A-BSD
    Tags
    P2A-BSD, SV40 bpNLS, and c-Myc NLS
    Expression
    Mammalian
    Mutation
    Detailed in manuscript
    Promoter
    CMV
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    174821-plasmid-map-sequence-id-343626

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Showing: 801 - 820 of 25285 results