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Viral Prep#140135-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV-EF1a-iCreV (#140135). In addition to the viral particles, you will also receive purified pAAV-EF1a-iCreV plasmid DNA. EF1a-driven, light-inducible iCreV recombinase for spatiotemporally precise optogenomic modifications. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aAvailable SinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only
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pAAV-EF1a-iFlpV (AAV PHP.eB)
Viral Prep#140137-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV-EF1a-iFlpV (#140137). In addition to the viral particles, you will also receive purified pAAV-EF1a-iFlpV plasmid DNA. EF1a-driven, light-inducible iFlpV recombinase for spatiotemporally precise optogenomic modifications. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aAvailable SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-iDreV (AAV PHP.eB)
Viral Prep#140136-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV-EF1a-iDreV (#140136). In addition to the viral particles, you will also receive purified pAAV-EF1a-iDreV plasmid DNA. EF1a-driven, light-inducible iDreV recombinase for spatiotemporally precise optogenomic modifications. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aAvailable SinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEcCas
Plasmid#73227PurposeConstitutive expression of cas9 and inducible expression of lambda RED and sgRNADepositorInsertsCas9
sgRNA
UseCRISPR; Red recombinaseExpressionBacterialPromoternative promoterAvailable SinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
HPV16 E6 TCR
Plasmid#122727PurposeExpress HPV16 E6 TCRDepositorInsertHPV16 E6 TCR
UseRetroviralAvailable SinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMP71-Flt3L
Plasmid#174600PurposeSecretes the extracellular domain of murine Flt3LDepositorInsertsecretes extracellular domain of mouse Flt3-L
ExpressionMammalianAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMP71-GM-CSF
Plasmid#174596Purposeexpresses secreted murine GM-CSFDepositorInsertGM-CSF
ExpressionMammalianAvailable SinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR_Puro_sgTP53
Plasmid#187819PurposepLentiCRISPR that is selectable with puromycin with an sgRNA targeting TP53DepositorInsertsgTP53
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
NW50
Plasmid#133445PurposeCovalently circularized nanodisc NW50 for studying membrane proteins and viral entry.DepositorInsertNW50
ExpressionBacterialAvailable SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAFNF-ECFP-Actb
Plasmid#169787PurposeAn actin FRET sensor. Must be expressed with Venus-actin for FRET.DepositorAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Wnt3A
Plasmid#35908DepositorAvailable SinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher003 S49C
Plasmid#133448PurposeExpresses SpyCatcher003 S49C in bacterial cytoplasm, with a unique cysteine for chemical couplingDepositorInsertSpyCatcher003 S49C
TagsGSSGS, His6, and TEV cleavage siteExpressionBacterialMutationContains S49C mutationPromoterT7Available SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX330-BsaIx2-DD-Cas9
Plasmid#75380PurposeExpresses DD-SpCas9 in mammalian cells and has a cloning site for an sgRNA. The FKBP12 L106P destabilization domain allows for inducible stabilization of Cas9 through the addition of Shield-1DepositorInsertDD-SpCas9
UseCRISPRTagsFKBP12 L106P DDExpressionMammalianPromoterCAGAvailable SinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-GCaMP6s-CAAX
Plasmid#52228PurposePlasma membrane targeted GCaMP6s, calcium sensorDepositorInsertGCaMP6s-CAAX
Tags6xHis and CAAXExpressionMammalianPromoterCMVAvailable SinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCas
Plasmid#62225PurposeConstitutive expression of cas9 and inducible expression of lambda RED and sgRDepositorHas ServiceCloning Grade DNAInsertcas9
UseCRISPR; Lambda red recombinaseExpressionBacterialPromoternative cas9 promoterAvailable SinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_mNG2(11)_Clathrin light chain
Plasmid#82608PurposeExpresses mNG2(11) tagged clathrin light chain in mammalian cellsDepositorInsertmNG2(11)_Clathrin light chain
ExpressionMammalianAvailable SinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAGM55261
Plasmid#153210PurposeLevel 2 cloning vector for a single guide RNA, already contains Cas9, Bar selection cassette and FAST markerDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_sfCherry2(11)_H2B
Plasmid#82605PurposeExpresses sfCherry2(11) tagged H2B in mammalian cellsDepositorInsertsfCherry2(11)_H2B
ExpressionMammalianMutationchanged Gly12 to Ala in sfCherry11+Available SinceJune 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHEN2-SG4 R105A
Plasmid#196072PurposeExpress mutant (R105A) SG4 in E.coliDepositorInsertSG4 R105A
Tags3XFLAG and 6XHisExpressionBacterialMutationR105A mutation in SG4PromoterT7Available SinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-EGFP
Plasmid#27082PurposeIntegration-free (episomal) expression of EGFPDepositorInserteGFP
ExpressionMammalianAvailable SinceMarch 10, 2011AvailabilityAcademic Institutions and Nonprofits only