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HA GSK3 beta S9A pcDNA3

Plasmid

#14754

Purpose

Mammalian expression of human GSK3 beta constitutively active mutant with HA tag

Depositor

Jim Woodgett

Article

Stambolic et al Biochem J. 1994 Nov 1;303 ( Pt 3)(Pt 3):

Insert

GSK3 beta S9A (GSK3B Human)

Tags

HA

Expression

Mammalian

Mutation

S9A. Constitutively active mutant.

Available Since

April 12, 2007

Availability

Industry, Academic Institutions, and Nonprofits

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beta-arrestin1 mYFP

Plasmid

#36916

Depositor

Robert Lefkowitz

Article

Violin et al J Biol Chem. 2006 Jul 21;281(29):20577-8

Insert

beta-arrestin1 (Arrb1 Rat)

Tags

mYFP

Expression

Mammalian

Promoter

CMV

Available Since

June 27, 2012

Availability

Academic Institutions and Nonprofits only

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GFP-Drosha

Plasmid

#62520

Purpose

wild-type human Drosha cDNA is subcloned into pEGFP-C1 vector between HindIII and KpnI sites

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha full length (DROSHA Human)

Tags

GFP

Expression

Mammalian

Available Since

March 20, 2015

Availability

Academic Institutions and Nonprofits only

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pHAGE-CMV-hAAT-W

Plasmid

#126704

Purpose

Constituitive, ubiquitious human alpha - 1 antitrypsin gene expression

Depositor

Darrell Kotton

Article

Wilson et al J Clin Invest. 2010 Jan . 120(1):379-89.

Insert

hAAT (SERPINA1 Human)

Use

Lentiviral

Promoter

CMVp

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pAG415GAL-ccdB

Plasmid

#14145

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway Cloning

Expression

Yeast

Available Since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP22

Plasmid

#22575

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP22 (USP22 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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Level 1 P2 OsUbiP:Cas9:NosT

Plasmid

#165424

Purpose

GoldenGate (MoClo) Level 1 Position 2 OsUbiP: Cas9: NosT cassette

Depositor

Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing

Article

Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

Insert

Os Ubiquitin promoter :: Cas9 :: NosTerminator

Use

Synthetic Biology

Promoter

Os Ubiquitin promoter :: Cas9 :: NosTerminator

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1 - TRC mTagBFP2

Plasmid

#191566

Purpose

Expresses an shRNA and mTagBFP2

Depositor

Timothy Ryan

Article

Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6 for shRNA

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pFCcGi

Plasmid

#59324

Purpose

Inducible expression of GFP and constitutive expression of mCherry

Depositor

Sophie Helaine, David Holden

Article

Figueira et al MBio. 2013 Apr 16;4(2):e00065. doi: 10.1

Inserts

mCherry
EGFP

Expression

Bacterial

Promoter

Pbad and rpsM

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-F-RAM-d2tTA-TRE-mKate2

Plasmid

#140274

Purpose

F-RAM, the Fos-dependent reporter

Depositor

Yingxi Lin

Article

Sun et al Cell. 2020 Apr 16;181(2):410-423.e17. do

Insert

mKate2

Use

AAV

Promoter

TRE

Available Since

July 27, 2021

Availability

Academic Institutions and Nonprofits only

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pMK265 (RAD21-mAC Hygro)

Plasmid

#140539

Purpose

RAD21 tagging with mAID-Clover

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

RAD21 (RAD21 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-SUMO-MarathonRT

Plasmid

#109029

Purpose

Group II intron maturase from Eubacterium rectale (wild-type sequence). This is a highly processive reverse transcriptase that is called “MarathonRT”

Depositor

Anna Pyle

Article

Zhao et al RNA. 2018 Feb;24(2):183-195. doi: 10.126

Insert

ERE_01490

Tags

6xHis-SUMO tag

Expression

Bacterial

Promoter

T7

Available Since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

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phL1A-pcDNA3

Plasmid

#12307

Depositor

Vance Lemmon

Article

Hlavin et al Genomics. 1991 Oct . 11(2):416-23.

Insert

L1CAM (L1CAM Human)

Expression

Mammalian

Available Since

Oct. 7, 2006

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA (AAV Retrograde)

Viral Prep

#154867-AAVrg

Purpose

Ready-to-use AAV Retrograde particles produced from pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA (#154867). In addition to the viral particles, you will also receive purified pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA plasmid DNA. Syn-driven, Flp-dependent hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

Depositor

Ulrik Gether

Article

Runegaard et al eNeuro. 2018 Jun 18;5(3). pii: eN-NWR-03

Promoter

Human Synapsin-1

Tags

mCherry (Flp-dependent)

Available Since

Oct. 8, 2020

Availability

Academic Institutions and Nonprofits only
ATP5F1B-pmCherry

Plasmid

#232783

Purpose

Encodes an mCherry-tagged ATP5F1B

Depositor

Giulia Bertolin

Article

Sharma et al Cell Death Discov. 2023 Jun 29;9(1):203.

Insert

ATP5F1B (ATP5F1B Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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Endy/Shearwin pOSIP plasmid set

Plasmid Kit

#1000000035

Purpose

Cloning and assembly of DNA constructs for chromosomal integration in E. coli.

Depositor

Drew Endy

Application

Cloning and Synthetic Biology

Vector Type

Bacterial Expression

Cloning Type

Clonetegration

Available Since

July 26, 2013

Availability

Academic Institutions and Nonprofits only
pdRBFC-B2-YN

Plasmid

#74721

Purpose

Express C-terminal YN fused flock house virus (FHV) B2 protein (2741-2956 nt) for dsRNA labeling

Depositor

Aiming Wang

Article

Cheng et al Virology. 2015 Nov;485:439-51. doi: 10.1

Insert

Influenza A virus NS1 protein, partial

Use

Double-stranded rna labeling

Tags

HA and YFP N-terminal half domain

Expression

Plant

Promoter

CaMV 35S

Available Since

May 9, 2016

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-mTurquoise2

Plasmid

#98886

Purpose

Produces a fusion between mTurquoise2 and mNeonGreen. It can be used as a positive control for FRET

Depositor

Dorus Gadella

Article

Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

Insert

mNeonGreen-mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pLYS5-SDHB-Flag

Plasmid

#50055

Purpose

Expression of human SDHB in mammalian cells

Depositor

Vamsi Mootha

Article

Sancak et al Science. 2013 Nov 14.

Insert

SDHB (SDHB Human)

Tags

Flag

Expression

Mammalian

Available Since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pGY037 BSMV-gamma-AmCyan

Plasmid

#234818

Purpose

To express flourescent protein AmCyan from the gamma genome of Barley Stripe Mosaic Virus Vector (T-DNA)

Depositor

Daniel Voytas

Article

Baysal et al Plant J. 2025 Jan;121(2):e17196. doi: 10

Insert

AmCyan

Expression

Plant

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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