178,447 results
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Plasmid#64622Purposewhen used to produce lentivirus, express physiological levels of insertDepositorAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by
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pLV-IRF9
Plasmid#71452PurposeLentiviral expression of IRF9DepositorAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p5E-mfap4
Plasmid#70052Purpose5' Entry vector containing the zebrafish mfap4 promoter. Used with Gateway Recombination to generate transgenic constructs exhibiting macrophage-restricted expression of transgenes.DepositorInsertmfap4 promoter
UseGateway 5' elementAvailable sinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141A
Plasmid#69290PurposeGateway entry vector; single gRNA under AtU6 promoterDepositorTypeEmpty backboneUseCRISPRAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_ManII-SBP-EGFP
Plasmid#65252Purposesynchronize trafficking of ManII from the ER (RUSH system)DepositorInsertStreptavidin-KDEL and truncated Mannosidase II
TagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianMutationcontains only amino acids 1 to 116 of ManIIPromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-EF1a-tdTomato-WPRE-pGHpA
Plasmid#67527Purpose"Expresses the fluorescent protein tdTomato"DepositorInserttandem dimer tomato
UseAAVExpressionMammalianPromoterEF1aAvailable sinceJuly 23, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3 Flag C/EBPb LAP
Plasmid#66979PurposeExpression plasmid for mouse C/EBPb LAP; adds Flag tag to the N-terminus of proteinDepositorAvailable sinceJuly 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKLV-U6gRNA-EF(BbsI)-PGKpuro2ABFP
Plasmid#62348PurposeAn empty optimized gRNA expression vectorDepositorInsertnone
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CaMKII-Jaws-KGC-GFP-ER2
Plasmid#65015PurposeAAV-mediated expression of Jaws under the CaMKII promoterDepositorInsertJaws-KGC-GFP-ER2
UseAAVTagsER2, GFP, and KGCExpressionMammalianMutationK200R W214FPromoterCaMKIIAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
th-P2A-EGFP donor
Plasmid#65562PurposeIntron targeting-mediated EGFP knockin at the zebrafish th locus.DepositorAvailable sinceJune 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV2-SEP-GluA1 (M1)
Plasmid#64942Purposemammalian expression of rat GluA1DepositorAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
TGFB1-bio-His
Plasmid#52185PurposeExpresses full-length Transforming growth factor beta-1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertTGFB1 (TGFB1 Human)
TagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBUN421
Plasmid#62204PurposeCRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (TaU3 promoter), Bar resistanceDepositorInsertsgRNA scaffold
zCas9
UsePlant binary vectorTags3x FLAG and NLSExpressionPlantMutationmaize codon optimizedPromoterTaU3 and UbiAvailable sinceFeb. 13, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSBbi-BP
Plasmid#60512PurposeSB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: BFP and puromycin resistance geneDepositorTypeEmpty backboneUseTransposonExpressionMammalianPromoterEF1a/RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTY-EF1a-wild type mKdm2b-Flag
Plasmid#61739PurposeExpress wild-type mouse histone demethylase Kdm2bDepositorAvailable sinceFeb. 6, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRK5-FLAG-USP5
Plasmid#61742Purposeexpress N-terminally FLAG tagged human USP5DepositorAvailable sinceFeb. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-V5-PTPN14-wild type
Plasmid#61003PurposeExpresses wild type human PTPN14 in mammalian cells, N-terminus V5 tagDepositorAvailable sinceNov. 20, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pQCXIP-GFP-LacR
Plasmid#59418Purposeretroviral construct expressing the Lac repressor (LacR) tagged with GFPDepositorInsertLac repressor (LacR)
UseRetroviralTagsGFPAvailable sinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
FRB_SCF2_LD6_CTEV
Plasmid#58879PurposeMESA split-TEV protease chain with Rapamycin FRB ectodomain 2 aa scaffold, 6 aa linker domain, C-terminal TEV proteaseDepositorPublicationInsertMESA split protease chain with FRB rapamycin-binding ectodomain and C terminal fragment of TEV protease
UseAAVExpressionMammalianPromoterCMVAvailable sinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
OCT4-GFP-2A-PURO transgenic reporter
Plasmid#52379PurposeBAC recombineering generated construct, used as a reporter for OCT4 expressionDepositorInsertsGFP-2A-puro
OCT4
ExpressionMammalianPromoterOCT4Available sinceOct. 10, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by