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pAG1159 TOM20-ECFP-FIREmate

Plasmid

#196693

Purpose

Expresses TOM20-ECFP-FIREmate in mammalian cells

Depositor

Arnaud Gautier

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

TOM20-ECFP-FIREmate

Tags

FIREmate

Expression

Mammalian

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-coilin

Plasmid

#36906

Depositor

Greg Matera

Article

Shpargel et al J Cell Sci. 2003 Jan 15;116(Pt 2):303-12

Insert

coilin (COIL Human)

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pCASP-SptP120-3m160-HilA

Plasmid

#153325

Purpose

Triggers secretion of SptP120 fused to the 3m160 anti-mCherry DARPin from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP120-3m160 DARPin

Use

Salmonella expression

Available Since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2 GFP-GSK3-MAPK

Plasmid

#29689

Purpose

GFP reporter protein fused to three GSK3 phosphorylation sites

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

GFP glycogen synthase kinase 3 recognition site primed by MAPK

Tags

Flag and Flag-EGFP

Expression

Mammalian

Mutation

recognition sites only, and E3 ligase site

Promoter

SP6

Available Since

July 7, 2011

Availability

Academic Institutions and Nonprofits only

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UBC plasmid

Plasmid

#169746

Purpose

Unregulated "open-loop" UBC promoter plasmid. This plasmid encodes eGFP to report circuit output levels.

Depositor

Francois St-Pierre

Article

Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

Insert

EGFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

UBC

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-YFP

Plasmid

#11180

Purpose

Mammalian expression vector for expression of YFP

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

Insert

EYFP from Aequorea victoria

Expression

Mammalian

Available Since

April 13, 2006

Availability

Academic Institutions and Nonprofits only

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pCGN-HCF-1 fl

Plasmid

#53309

Purpose

plasmid contains human HCFC1 full length gene with HA and c-myc tags

Depositor

Winship Herr

Article

Wilson et al Cell. 1993 Jul 16;74(1):115-25.

Insert

HCFC1 (HCFC1 Human)

Tags

HA and c-myc

Expression

Mammalian

Promoter

CMV

Available Since

May 28, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV_BiSSTe10_ChR2_mCherry (AAV PHP.eB)

Viral Prep

#213815-PHPeB

Purpose

Ready-to-use AAV PHP.eB particles produced from pAAV_BiSSTe10_ChR2_mCherry (#213815). In addition to the viral particles, you will also receive purified pAAV_BiSSTe10_ChR2_mCherry plasmid DNA. Expression of mCherry-tagged channelrhodopsin-2 under the control of the SST interneuron-targeting enhancer E10. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

Depositor

Gordon Fishell

Article

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Tags

mCherry

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only
mScarletI-Rab7

Plasmid

#112955

Purpose

In vivo visualization of Rab7 (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Rab7

Tags

mScarlet-I

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2018

Availability

Academic Institutions and Nonprofits only

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PMKO-shNC

Plasmid

#64863

Purpose

Expresses a scrambled shRNA

Depositor

Lei Sun

Article

Alvarez-Dominguez et al Cell Metab. 2015 May 5;21(5):764-76. doi

Insert

Scrambled shRNA

Use

Retroviral

Promoter

u6

Available Since

May 26, 2015

Availability

Academic Institutions and Nonprofits only
pCAG-HA-Nrxn1beta AS4(+)

Plasmid

#59410

Purpose

Expression plasmid for HA-tagged mouse Neurexin 1beta AS4(+) [containing the insert at alternatively spliced segment 4 (AS4+)]

Depositor

Peter Scheiffele

Article

Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

Insert

Nrxn1beta AS4(+) (Nrxn1 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CAG

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pTRE-tdTomato

Plasmid

#50798

Purpose

Expression of tdTomato under the control of the TRE-promoter

Depositor

Connie Cepko

Article

Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

Insert

TREtight driven tdTomato fluorescent protein

Use

Mutli-species

Promoter

TREtight

Available Since

March 20, 2014

Availability

Academic Institutions and Nonprofits only

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pBRPB CAG-mCherry-IP

Plasmid

#106333

Purpose

PiggyBac transposon, mCherry-IRES-puro labeling under the control of CAG promoter. Used in chimera experiment of ES cells.

Depositor

Thomas Tuschl

Article

Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

Insert

mCherry-IRES-Puro

Use

Piggybac transposon

Expression

Mammalian

Promoter

CAG

Available Since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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EYFP-TRAF2

Plasmid

#111203

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Article

Birbach et al J Biol Chem. 2002 Mar 29;277(13):10842-5

Insert

TRAF2 (TRAF2 Human)

Tags

EYFP

Expression

Mammalian

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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sgRNA BRCA2 R2842C

Plasmid

#139324

Purpose

Plasmid expressing a sgRNA to introduce BRCA2 R2842C using base editing

Depositor

Alberto Ciccia

Article

Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

Insert

sgRNA to insert BRCA2 R2842C using base editing

Expression

Mammalian

Promoter

U6

Available Since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

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pDZ305 GTL1 GAL1p 24xPP7 V3 <HIS>

Plasmid

#72240

Purpose

Histidine selection; Used to insert a GAL1 promoter and 24 repetitive PP7 version 3 RNA stem-loops before GLT1's start codon (5' tagging)

Depositor

Daniel Zenklusen

Article

Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

Insert

GLT1 GAL1p 24xPP7 V3

Expression

Bacterial

Available Since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pTL032

Plasmid

#80928

Purpose

14x MS2 sense + 14x PP7 antisense with loxP-kanMX-loxP

Depositor

Daniel Larson

Article

Lenstra et al Mol Cell. 2015 Nov 19;60(4):597-610. doi

Insert

14xMS2 sense + 14xPP7 antisense, loxP-kanMX-loxP

Expression

Bacterial

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pLV hUbC-dCas9-T2A-GFP

Plasmid

#53191

Purpose

Co-expresses human optimized S. pyogenes dCas9 and GFP

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Insert

humanized dead Cas9 T2A GFP

Use

CRISPR and Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

D10A and H840A

Promoter

hUbC

Available Since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

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pNB655

Plasmid

#60142

Purpose

Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by MET17 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc-yEVenus

Expression

Yeast

Mutation

A4V

Promoter

MET17pr

Available Since

Feb. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA6 asyn WT

Plasmid

#107425

Purpose

Mammalian expression of human alpha synuclein

Depositor

Hilal Lashuel

Article

Mbefo et al J Biol Chem. 2015 Apr 10;290(15):9412-27

Insert

alpha-synuclein (SNCA Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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