167,948 results

1. pAAV.Syn.NES.jRCaMP1a.WPRE.SV40

   Plasmid

   #100848

   Purpose

   AAV expression of jRCaMP1a, a red fluorescent calcium sensor protein, from the Synapsin promoter

   Depositor

   GENIE Project, Douglas Kim

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Has Service

   AAV1 and AAV9

   Insert

   jRCaMP1a
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Synapsin

   Available Since

   Nov. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRS416-dCas9-Mxi1 + TetR + pRPR1(TetO)-NotI-gRNA

   Plasmid

   #73796

   Purpose

   pRS416 Ura marked Cen/Ars plasmid with dCas9-Mxi1 under Tef1 promoter, and tet-incucibile RPR1 promoter with NotI cloning site adjacent to gRNA

   Depositor

   Ronald Davis

   Article

   Smith et al Genome Biol. 2016 Mar 8;17(1):45. doi: 1

   Inserts

   dCas9-Mxi1
   Tet Repressor
   Structural gRNA for S pyogenes
   

   Use

   CRISPR

   Tags

   Mxi1 and NLS

   Expression

   Yeast

   Mutation

   D10A, H840A

   Promoter

   pGPM1, pRPR1(TetO), and pTef1

   Available Since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBS-SKII-3XHA-fCLIP-NAT

   Plasmid

   #188921

   Purpose

   Integration cassette to create C-terminal fusions for fluorescent tagging

   Depositor

   Stephen Buratowski

   Article

   Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

   Insert

   3XHA-fCLIP-NAT
   

   Expression

   Yeast

   Available Since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBMN mEGFP-OPTN

   Plasmid

   #188784

   Purpose

   For mammalian expression of OPTN tagged with mEGFP

   Depositor

   Michael Lazarou

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   OPTN (OPTN Human)
   

   Use

   Retroviral

   Tags

   mEGFP

   Expression

   Mammalian

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. IDG_CACNG3_OE_1

   Plasmid

   #161622

   Purpose

   Overexpresses CACNG3

   Depositor

   Mike McManus

   Insert

   CACNG3 (CACNG3 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Jan. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. CROP_C9_Puro_PDGFRA

   Plasmid

   #183313

   Purpose

   All-in-One CRISPRko system with a guide RNA that targets PDGFRA gene

   Depositor

   Prashant Mali

   Article

   Hu et al Biomaterials. 2022 Jan;280:121276. doi:

   Insert

   PDGFRA
   

   Use

   Lentiviral

   Available Since

   May 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-ABE7.10

   Plasmid

   #102919

   Purpose

   expresses ABE7.10 in mammalian cells

   Depositor

   David Liu

   Article

   Gaudelli et al Nature. 2017 Nov 23;551(7681):464-471. d

   Insert

   ABE7.10
   

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   see manuscript

   Promoter

   CMV

   Available Since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-Clover

   Plasmid

   #40259

   Purpose

   GFP with robust performance in protein fusions and useful as FRET donor for mRuby2 in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlap

   Depositor

   Michael Lin

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Has Service

   Cloning Grade DNA

   Insert

   Clover GFP
   

   Expression

   Mammalian

   Promoter

   CMV IE

   Available Since

   Sept. 24, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA HA DUSP6

   Plasmid

   #236430

   Purpose

   transient overexpression of DUSP6 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Duan et al Cell Rep. 2021 Oct 19;37(3):109870. doi:

   Insert

   DUSP6 (DUSP6 Human)
   

   Expression

   Mammalian

   Available Since

   May 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pIG-601_NY-ESO-1_TCR_GFP

    Plasmid

    #207482

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    GFP, NY-ESO-1_TCR
    

    Use

    CRISPR

    Mutation

    Potential silent point mutation in GFP sequence

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. Str-KDEL_neomycin

    Plasmid

    #65306

    Purpose

    Luminal ER hook only (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Streptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end
    

    Tags

    KDEL motif and Signal Sequence

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. HBV 1.3-mer X-null replicon

    Plasmid

    #65461

    Purpose

    containing 1.3 units of the X-null HBV genome (subtype ayw)

    Depositor

    Wang-Shick Ryu

    Article

    Hussain et al J Gen Virol. 2009 Sep;90(Pt 9):2272-81.

    Insert

    1.3 units of the X-null HBV genome
    

    Expression

    Mammalian

    Available Since

    Dec. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOT_6 - lenti-EFS-FMC6.3-BBz-2A-puro-2A-tNGFR

    Plasmid

    #181975

    Purpose

    Expresses anti-CD19 CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A and to human truncated NGFR via T2A for lentiviral delivery.

    Depositor

    Neville Sanjana

    Article

    Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

    Insert

    FMC6.3-BBz CAR; tNGFR (NGFR Synthetic, Human)
    

    Use

    Lentiviral

    Mutation

    NGFR truncation: aa 11-276

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT3TS-spRYCas9-ABE-UMax

    Plasmid

    #222139

    Purpose

    Near PAMless Adenine base editor with modified TadA variant for A-to-G base editing in zebrafish

    Depositor

    Gaurav Varshney

    Article

    Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

    Insert

    TadA-nSpRYCas9
    

    Use

    CRISPR; Zebrafish expression

    Tags

    bipartite NLS

    Promoter

    T3

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. p11-LacY-wtx1

    Plasmid

    #69056

    Purpose

    The reporter plasmid p11-LacY-wtx1 encodes the toxin CcdB gene under the inducible BAD promoter and a single copy of the endonuclease cleavage site which is readily extendable to multiple copies.

    Depositor

    Huimin Zhao

    Article

    Chen et al Nucleic Acids Res. 2005 Oct 6;33(18):e15

    Inserts

    ccdB (ccdB )
    lacY (lacY )
    

    Tags

    A177C

    Promoter

    pBAD and pLac

    Available Since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. Stat3-C Flag pRc/CMV

    Plasmid

    #8722

    Depositor

    Jim Darnell

    Article

    Bromberg et al Cell 1999 Aug 6;98(3):295-303.

    Insert

    Stat3-C (Stat3 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    A662C and N664C. Residues changed to Cys renders…

    Available Since

    Sept. 26, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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17. p35S_GFP-GUS

    Plasmid

    #167122

    Purpose

    Plant binary expression vector containing mGFP5-GUS fusion reporter under CaMV 35S promoter.

    Depositor

    Olivier Voinnet, Arturo Marí-Ordóñez

    Article

    Oberlin et al Genome Res. 2017 Sep;27(9):1549-1562. do

    Insert

    GFP-GUS
    

    Tags

    mGFP5, GUS

    Expression

    Plant

    Promoter

    CaMV 35S

    Available Since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLinker-BirA-3xHA-HXGPRT-loxP

    Plasmid

    #86668

    Purpose

    for generation of BirA tagged strains using CRISPR tagging technology

    Depositor

    David Sibley

    Insert

    Linker-BirA-3xHA-HXGPRT 3'UTR
    

    Expression

    Bacterial

    Available Since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. PKC delta DN

    Plasmid

    #16389

    Depositor

    Bernard Weinstein

    Article

    Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

    Insert

    PKC delta isoform 2 (Prkcd Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Dominant negative (kinase inactive): K376R

    Available Since

    April 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. cEF.tk-GFP

    Plasmid

    #33308

    Depositor

    Linzhao Cheng

    Article

    Pomper et al Cell Res. 2009 Mar;19(3):370-9.

    Insert

    HSV-TK/GFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    March 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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