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Showing: 2801 - 2850 of 11733 results

  19. PMmEos3.2

    Plasmid
    #203755
    Purpose
    Encodes the membrane anchor of Lyn including the first 10 residues of the N terminus, with 1 myristoylation and 1 palmitoylation, with mEos3.2 fused to the C terminus. Used as a fluorescent plasma membrane probe.
    Depositor
    Sarah Veatch
    Article
    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d
    Insert
    Lyn n-term (LYN Human)
    Tags
    mEos3.2
    Expression
    Mammalian
    Available Since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    203755-plasmid-map-sequence-id-403338

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  20. pOPS0379

    Plasmid
    #115510
    Purpose
    Suitable for experiments in environments where there is no antibiotic selection present. Symbiosis-induced nifH promoter from Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing sfGFP
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    Rlv3841pNifH(pOGG082) sfGFP (pOGG037) and T-pharma (pOGG003) assembled in pOGG026
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Available Since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    115510-plasmid-map-sequence-id-224287

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  22. pOPS0314

    Plasmid
    #115507
    Purpose
    Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing celB
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    celB pOGG050, T-pharma pOGG003 assembled in pOGG026
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Promoter
    Constitutive promoter pNeo pOGG001
    Available Since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    115507-plasmid-map-sequence-id-224284

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  28. pAAV-CMV-FRT-myrSNAP-2A-H2BeGFP-WPRE

    Plasmid
    #102986
    Purpose
    AAV expressing membrane-targeted SNAP protein and nuclear-targeted eGFP after FLP-mediated recombination
    Depositor
    Marco Tripodi
    Article
    Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306
    Inserts
    membrane-targeted SNAP protein after FLP-mediated recombination
    Nuclear-targeted eGFP after FLP-mediated recombination
    Use
    AAV; Adeno associated viral vector
    Available Since
    Dec. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    102986-plasmid-map-sequence-id-200006

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  29. pmirGLO-Smad4-1719

    Plasmid
    #49383
    Purpose
    Insert is a fragment of the Smad4 3'-UTR that contains miR-17 and miR-19 binding sites. To be used for 3'UTR assay.
    Depositor
    Martine Roussel
    Article
    Murphy et al Cancer Res. 2013 Oct 21.
    Insert
    Smad family member 4 (SMAD4 Mouse, Human)
    Use
    Luciferase
    Promoter
    Human phosphoglycerate kinase (PGK) promoter
    Available Since
    Oct. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    49383-plasmid-map-sequence-id-73406

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  32. pGS1021

    Plasmid
    #59575
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Twin-Strep tag and a TRP1 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    Twin-Strep tag optimized for PCR amplification followed by TRP1 selection marker
    Use
    E. coli cloning vector
    Tags
    Twin-Strep tag
    Promoter
    None
    Available Since
    Aug. 28, 2014
    Availability
    Academic Institutions and Nonprofits only
    59575-plasmid-map-sequence-id-92981

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  33. pGS1022

    Plasmid
    #59576
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Twin-Strep tag and a URA3 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    Twin-Strep tag optimized for PCR amplification followed by URA3 selection marker
    Use
    E. coli cloning vector
    Tags
    Twin-Strep tag
    Promoter
    None
    Available Since
    Aug. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    59576-plasmid-map-sequence-id-92983

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  38. pOPS0359

    Plasmid
    #115508
    Purpose
    Reporter Plasmid. Taurine-inducible expression of GFP
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    sfGFP pOGG037, T-pharma pOGG003 assembled in pOGG024
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Promoter
    Taurine-inducible promoter of S. meliloti Promote…
    Available Since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    115508-plasmid-map-sequence-id-224277

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  39. C-BLa 1.1

    Plasmid
    #88997
    Purpose
    BLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.1 fusionprotein containing the C-terminal fragment of the split β-lactamase.
    Depositor
    Dieter Langosch
    Article
    Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103
    Insert
    C-BLa 1.1 fusion protein
    Tags
    Flag
    Expression
    Bacterial
    Promoter
    araBAD
    Available Since
    May 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    88997-plasmid-map-sequence-id-177504

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  42. pN_35S/mCitrine/P_UBQ10/Derlin1-mOrange2

    Plasmid
    #118000
    Purpose
    Co-expression of mCitrine under the control of the CaMV 35S promoter and Derlin1 fused to mOrange2 (an ER-targeting marker protein) under the control of the A. thaliana ubiquitin-10 promoter.
    Depositor
    Mathias Pribil
    Article
    Behrendorff et al ACS Synth Biol. 2019 Mar 18. doi: 10.102
    Insert
    Derlin1-mOrange2
    Use
    Plant/bacteria binary vector
    Expression
    Plant
    Promoter
    Ubiquitin-10
    Available Since
    March 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    118000-plasmid-map-sequence-id-229409

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Showing: 2801 - 2850 of 11733 results