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20,449 results

Showing: 2951 - 3000 of 20449 results

  1. lentiMPH v2 (Neo)

    Plasmid
    #92065
    Purpose
    Modified version of lentiMPH v2, a lenti vector encoding the MS2-P65-HSF1 activator helper complex and neo resistance marker (EF1a-MS2-p65-HSF1-2A-Neo-WPRE).
    Depositor
    Adam Karpf
    Article
    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:
    Insert
    MS2-P65-HSF1_2A_Neo (HSF1 Synthetic, Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    N55K in MS2
    Promoter
    EF1a
    Available since
    June 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    92065-plasmid-map-sequence-id-181210

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  4. pVP91A-pcaHG

    Plasmid
    #113766
    Purpose
    Expresses the alpha (pcaG) and the beta (pcaH) subunits of Protocatechuate 3,4-Dioxygenase from Pseudomonas putida.
    Depositor
    John Lipscomb
    Article
    Knoot et al Proc Natl Acad Sci U S A. 2015 Jan 13;11
    Inserts
    β subunit of Protocatechuate 3,4- Dioxygenase
    α subunit of Protocatechuate 3,4- Dioxygenase
    Use
    Tags
    Met-6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    T5 promoter
    Available since
    Aug. 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    113766-plasmid-map-sequence-id-218346

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  5. pLSL

    Plasmid
    #51493
    Purpose
    T-DNA destination plasmid. Contains the bean yellow dwarf virus cis-acting elements (LIR and SIR) in an LIR-SIR-LIR orientation. Contains attR1 and attR2 sites between the LIR and SIR sequence.
    Depositor
    Daniel Voytas
    Article
    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:
    Type
    Empty backbone
    Use
    Plant t-dna plasmid
    Tags
    Expression
    Mutation
    Promoter
    virion-sense LIR and 2x35S
    Available since
    May 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    51493-plasmid-map-sequence-id-78588

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  10. pAAV_hSyn1-SIO-stGtACR1-FusionRed (AAV5)

    Viral Prep
    #105678-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV_hSyn1-SIO-stGtACR1-FusionRed (#105678). In addition to the viral particles, you will also receive purified pAAV_hSyn1-SIO-stGtACR1-FusionRed plasmid DNA. Synapsin-driven, Cre-dependent, soma-targeted anion-conducting channelrhodopsin fused to FusionRed for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ofer Yizhar
    Article
    Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1
    Promoter
    Syn
    Tags
    FusionRed (Cre-dependent)
    Available since
    Sept. 24, 2020
    Availability
    Academic Institutions and Nonprofits only

  12. pAAV asyn S129A

    Plasmid
    #36066
    Depositor
    Hilal Lashuel, Patrick Aebischer
    Insert
    alpha-synuclein S129A (SNCA Human)
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    S129A
    Promoter
    CMV
    Available since
    Sept. 10, 2012
    Availability
    Academic Institutions and Nonprofits only

  21. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA

    Plasmid
    #182653
    Purpose
    Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF),tRNACUAPyl & eRF1E55D used for amber codon suppression in mammalian cells
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Inserts
    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    mutant eukaryotic release factor 1
    Use
    Tags
    HA tag and nuclear export signal (NES)
    Expression
    Mammalian
    Mutation
    E55D and Y306A/Y384F (AF) double mutant of Methan…
    Promoter
    CMV and U6
    Available since
    June 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    182653-plasmid-map-sequence-id-363026

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  24. pTC223

    Plasmid
    #70019
    Purpose
    Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 7
    Depositor
    Daniel Voytas
    Article
    Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:
    Insert
    Nuclease (Cas9/sgRNA) + Donor + GVR
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Dec. 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    70019-plasmid-map-sequence-id-124558

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  29. pTrex-n-eGFP

    Plasmid
    #62544
    Purpose
    Expression of eGFP in T. cruzi. The NheI-linearized form of this plasmid could be efficiently integrated in to T. cruzi genome
    Depositor
    Rick Tarleton
    Article
    Peng et al MBio. 2014 Dec 30;6(1). pii: e02097-14.
    Insert
    eGFP
    Use
    (1)trypanosoma cruzi expression of egfp (2) genom…
    Tags
    None
    Expression
    Mutation
    None
    Promoter
    Ribo-HX1
    Available since
    April 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    62544-plasmid-map-sequence-id-109759

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  30. pCMV-Neo-Bam

    Plasmid
    #16440
    Purpose
    Contains two independent transcription units--a CMV promoter/enhancer upstream of a MCS and a HSV thymidine kinase promoter/enhancer upstream of the neomycin resistance gene.
    Depositor
    Bert Vogelstein
    Article
    Baker et al Science. 1990 Aug 24. 249(4971):912-5.
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 7, 2008
    Availability
    Academic Institutions and Nonprofits only
    16440-plasmid-map-sequence-id-211955

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  31. pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (AAV Retrograde)

    Viral Prep
    #20298-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (#20298). In addition to the viral particles, you will also receive purified pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA plasmid DNA. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    EF1a
    Tags
    EYFP (Cre-dependent)
    Available since
    Oct. 12, 2017
    Availability
    Academic Institutions and Nonprofits only

  37. psd44-GqQL

    Plasmid
    #46826
    Purpose
    2nd generation lentiviral vector for expression of mutant G protein alpha q in mammalian cells
    Depositor
    Agnese Mariotti
    Insert
    g protein alpha q (GNAQ Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Q209L (The mutation reduces GTPase activity resul…
    Promoter
    PGK promoter
    Available since
    July 30, 2013
    Availability
    Academic Institutions and Nonprofits only
    46826-plasmid-map-sequence-id-246440

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  38. MSCV IRES Luciferase

    Plasmid
    #18760
    Depositor
    Scott Lowe
    Type
    Empty backbone
    Use
    Luciferase and Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 3, 2008
    Availability
    Academic Institutions and Nonprofits only
    18760-plasmid-map-sequence-id-169127

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  40. 4xNFkB Luc

    Plasmid
    #111216
    Purpose
    reporter gene
    Depositor
    Johannes A. Schmid
    Insert
    firefly Luciferase
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    111216-plasmid-map-sequence-id-213082

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  48. pAAV-hDlx-GiDREADD-dTomato-Fishell-5 (AAV1)

    Viral Prep
    #83896-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-hDlx-GiDREADD-dTomato-Fishell-5 (#83896). In addition to the viral particles, you will also receive purified pAAV-hDlx-GiDREADD-dTomato-Fishell-5 plasmid DNA. hDlx-driven expression of Gi-DREADD and nuclear localized dTomato (physically separate). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Gordon Fishell
    Article
    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/
    Promoter
    hDlx
    Tags
    dTomato (physically separate, not a fusion protein)
    Available since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 2951 - 3000 of 20449 results