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We narrowed to 9,194 results for: sgRNA

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Showing: 121 - 140 of 9194 results

  1. eSpCas9(1.1)_No_FLAG_ATP1A1_G2_Dual_sgRNA

    Plasmid
    #86612
    Purpose
    Vector for tandem expression of ATP1A1 G2 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G2 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via nhej using ouabain
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available Since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86612-plasmid-map-sequence-id-168849

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  16. pLKO.1-puro U6 sgRNA BfuAI large stuffer

    Plasmid
    #52628
    Purpose
    lentiviral U6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sites, improved cassette cloning efficiency
    Depositor
    Scot Wolfe
    Insert
    Kanamycin cassette
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    April 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    52628-plasmid-map-sequence-id-217983

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  17. pSpCas9(BB)-2A-Neo

    Plasmid
    #127762
    Purpose
    Used for expression of sgRNA in mammalian cells
    Depositor
    Ken-Ichi Takemaru
    Type
    Empty backbone
    Expression
    Mammalian
    Available Since
    June 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    127762-plasmid-map-sequence-id-247554

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  19. pSpCas9(BB)-2A-Hygro

    Plasmid
    #127763
    Purpose
    Used for expression of sgRNA in mammalian cells
    Depositor
    Ken-Ichi Takemaru
    Type
    Empty backbone
    Expression
    Mammalian
    Available Since
    June 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    127763-plasmid-map-sequence-id-247560

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Showing: 121 - 140 of 9194 results