We narrowed to 9,004 results for: sgRNA

Showing: 121 - 140 of 9004 results

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pColE1_sgRNA_T2_AmpR

Plasmid

#119159

Purpose

Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

Use

Crispr

Promoter

J23119

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_T3_AmpR

Plasmid

#119160

Purpose

Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

Use

Crispr

Promoter

J23119

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_T1_AmpR

Plasmid

#119158

Purpose

Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

Use

Crispr

Promoter

J23119

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_M1NS_AmpR

Plasmid

#119155

Purpose

Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with one mismatch in the non-seed region

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with one mismatch in the non-seed region

Use

Crispr

Promoter

J23119

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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EC2_7_dCas9_HC_sgRNA

Plasmid

#163711

Purpose

High copy plasmid with dCas9 and sgRNA expression cassettes

Depositor

Yvonne Nygård

Article

Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

Insert

dCas9

Expression

Yeast

Mutation

Point mutations D10A and H840A

Promoter

ScTEF1

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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U6_sgRNA_CAG_ABE7.10_St1Cas9_LMD9

Plasmid

#136660

Purpose

Expresses ABE St1Cas9 LMD9 in mammalian cells along with its U6-driven sgRNA

Depositor

Yannick Doyon

Article

Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

Inserts

St1Cas9 LMD-9
sgRNA for St1Cas9
ABE7.10

Use

CRISPR and Synthetic Biology

Tags

SV40 NLS and hSt1Cas9

Expression

Mammalian

Promoter

CAG and U6

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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sgRNA4_ASCL1

Plasmid

#64132

Purpose

Photoactivatable transcription system. Lentiviral expression of ASCL1 sgRNA4. Also contains a CMV-puro-t2A-mCherry expression cassette.

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA1_ASCL4 (ASCL1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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sgRNA3_ASCL1

Plasmid

#64131

Purpose

Photoactivatable transcription system. Lentiviral expression of ASCL1 sgRNA3. Also contains a CMV-puro-t2A-mCherry expression cassette.

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA1_ASCL3 (ASCL1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_M4NS_AmpR

Plasmid

#119156

Purpose

Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with four mismatches in the non-seed region

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with four mismatches in the non-seed region

Use

Crispr

Promoter

J23119

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_pos9_AmpR

Plasmid

#119152

Purpose

Encodes sgRNA targeting position 9 in pColE1_70a_deGFP_KanR

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 9 in pColE1_70a_deGFP_KanR

Use

Crispr

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_G3BP2_G1

Plasmid

#127112

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

gRNA G3BP2 (G3BP2 Human)

Use

CRISPR

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pLCKO_PSMD1_sgRNA_5

Plasmid

#74181

Purpose

lentiviral vector expressing sgRNA targeting PSMD1

Depositor

Jason Moffat

Article

Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

Insert

PSMD1 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 Promoter

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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pNOC_dCas9_sgRNA NC

Plasmid

#176259

Purpose

pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and spacer sequence on sgRNA is replaced by the type IIS restriction site for endonuclease BaeI and

Depositor

John van der Oost

Article

Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

Insert

dead spCas9

Use

CRISPR, Luciferase, and Synthetic Biology ; Expre…

Tags

luciferase

Mutation

H840A and D10A mutations on spCas9 to inactivate …

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pLCKO_PSMD1_sgRNA_1

Plasmid

#74180

Purpose

lentiviral vector expressing sgRNA targeting PSMD1

Depositor

Jason Moffat

Article

Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

Insert

PSMD1 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 Promoter

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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sgRNA1_IL1RN

Plasmid

#64140

Purpose

Photoactivatable transcription system. Lentiviral expression of IL1RN sgRNA1. Also contains a CMV-puro-t2A-mCherry expression cassette

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

IL1RN (IL1RN Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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LCV2_mClover3_AAVS1_sgRNA_2

Plasmid

#155099

Purpose

lentiviral plasmid expressing mClover3, Cas9 and a gRNA targeting the AAVS1 safe harbor locus

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

AAVS1_sgRNA_2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCBLsgRNA3

Plasmid

#199729

Purpose

Golden Gate entry vector; 3rd gRNA under TaU3 promoter

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

TaU3-sgRNA3 scaffold

Expression

Plant

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCBLsgRNA4

Plasmid

#199730

Purpose

Golden Gate entry vector; 4th gRNA under ZmU6-C1 promoter

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

ZmU6C1-sgRNA4 scaffold

Expression

Plant

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCBLsgRNA5

Plasmid

#199731

Purpose

Golden Gate entry vector; 5th gRNA under ZmU6-C3 promoter

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

ZmU6C3-sgRNA5 scaffold

Expression

Plant

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCBLsgRNA6

Plasmid

#199732

Purpose

Golden Gate entry vector; 6th gRNA under OsU6.1 promoter

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

OsU6.1-sgRNA6 scaffold

Expression

Plant

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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