We narrowed to 15,981 results for: LEA

1. pJH27_pBad_AraC_Para-sfGFP.2TAG

   Plasmid

   #163722

   Purpose

   Arabinose-inducible expression of green fluorescent protein encoding two amber codons

   Depositor

   Matthew Bennett

   Article

   Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

   Insert

   araC gfp.2TAG
   

   Expression

   Bacterial

   Mutation

   gfp.N39TAG,Y151TAG

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJH25_pBad_AraC_Para-sfGFP.0TAG

   Plasmid

   #163720

   Purpose

   Arabinose-inducible expression of green fluorescent protein encoding zero amber codons

   Depositor

   Matthew Bennett

   Article

   Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

   Insert

   araC gfp
   

   Expression

   Bacterial

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTORKm43GW

   Plasmid

   #112897

   Purpose

   Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to geneticin (G418).

   Depositor

   Sebastian Schornack

   Article

   Evangelisti et al MBio. 2019 Oct 1;10(5). pii: mBio.01516-

   Type

   Empty backbone

   Use

   Oomycete expression

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28b-MseLig1

   Plasmid

   #107071

   Purpose

   Expresses M. sedula DNA Ligase 1 in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

   Insert

   DNA ligase 1
   

   Tags

   His6 tag

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFH42

   Plasmid

   #125881

   Purpose

   Level0 Cestrum Yellow Leaf Curling Virus CmYLCV Pol II promoter

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   Level0 Cestrum Yellow Leaf Curling Virus CmYLCV Pol II promoter
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PSuperPuroRetro-1660s(hPot1)

   Plasmid

   #11136

   Depositor

   Christopher Counter

   Article

   Veldman et al Curr Biol. 2004 Dec 29. 14(24):2264-70.

   Insert

   RNAi against Human Protection of Telomeres 1 (control) (POT1 Human)
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Available Since

   March 3, 2006

   Availability

   Academic Institutions and Nonprofits only

7. pBM13h-sd4U

   Plasmid

   #179690

   Purpose

   Bacterial expression of base editor

   Depositor

   David Liu

   Article

   Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

   Insert

   [sd4U] UGI-TALE3-DddA-G1397-N wildtype
   

   Expression

   Bacterial

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pQE30 NFAT5 DBD

   Plasmid

   #13629

   Depositor

   Anjana Rao

   Article

   Lopez-Rodriguez et al Proc Natl Acad Sci U S A. 1999 Jun 22. 9

   Insert

   NFAT5 (NFAT5 Human)
   

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   DNA binding domain: aa 175 to 471

   Available Since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

9. pBABEPuro-rnSOX9

   Plasmid

   #63009

   Purpose

   Expresses rat SOX9

   Depositor

   Michael Ruppert

   Article

   Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

   Insert

   SOX9 (Sox9 Rat)
   

   Use

   Retroviral

   Tags

   HA

   Available Since

   May 28, 2015

   Availability

   Academic Institutions and Nonprofits only

10. Venus-7aa-Cb5-pEGFP-C1

    Plasmid

    #166764

    Purpose

    To target Venus to the Endoplasmic Reticulum. Cb5 tail anchor inserts into the membrane and Venus faces the cytosol. This serves as a collisional control in FLIM-FRET experiments.

    Depositor

    David Andrews

    Insert

    Cb5 tail anchor (Cyb5a Rat)
    

    Tags

    Venus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFREE2-eSpCas9-sgRNA

    Plasmid

    #179580

    Purpose

    Expresses eSpCas9 and gRNA that targets ColE1 plasmids for curing

    Depositor

    Kevin Solomon

    Article

    Lee et al ACS Synth Biol. 2022 Jan 21;11(1):53-60.

    Insert

    eSpCas9
    

    Expression

    Bacterial

    Available Since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHJ386

    Plasmid

    #20679

    Depositor

    Stephen Shaw

    Article

    Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

    Insert

    Moesin (MSN Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Changed Lys 253, 254, 262, 263 to Asn; Changed Th…

    Available Since

    April 28, 2009

    Availability

    Academic Institutions and Nonprofits only

13. pc3XB-ZF70

    Plasmid

    #13193

    Depositor

    Daniel Voytas

    Article

    Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

    Insert

    ZF70
    

    Expression

    Mammalian

    Available Since

    Oct. 31, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Venus-7aa-MAO-pEGFP-C3

    Plasmid

    #166765

    Purpose

    To target Venus to the mitochondrial outer membrane. MAO tail anchor inserts into the outer membrane and Venus faces the cytosol. Serves as a collisional control in FLIM-FRET experiments.

    Depositor

    David Andrews

    Insert

    MAO tail anchor (MAOA Human)
    

    Tags

    Venus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTAL4

    Plasmid

    #31035

    Depositor

    Daniel Voytas, Adam Bogdanove

    Article

    Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

    Insert

    TALEN-FokI nuclease backbone
    

    Tags

    AcV5 and FokI nuclease

    Available Since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pXCX-sGFAP-LMO-IRES-tdTomato

    Plasmid

    #178312

    Purpose

    Express the bacterial lactate 2-monooxygenase (LMO) enzyme in astrocytes

    Depositor

    Sergey Kasparov

    Article

    Vaccari Cardoso et al Brain Sci. 2021 Aug 10;11(8). pii: brain

    Insert

    Lactate 2-monooxygenase
    

    Use

    Adenoviral

    Tags

    tdTomato

    Expression

    Mammalian

    Promoter

    sGFAP

    Available Since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJH807_pJH_LldR_Pcon-sfGFP.2TAG

    Plasmid

    #163738

    Purpose

    Constitutive expression of green fluorescent protein encoding two stop codons

    Depositor

    Matthew Bennett

    Article

    Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

    Insert

    lldr gfp.2TAG
    

    Expression

    Bacterial

    Mutation

    gfp.N39TAG,Y135TAG

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJH809_pJH_LldR_Pcon-sfGFP.10TAG

    Plasmid

    #163739

    Purpose

    Constitutive expression of green fluorescent protein encoding ten stop codons

    Depositor

    Matthew Bennett

    Article

    Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

    Insert

    lldr gfp.10TAG
    

    Expression

    Bacterial

    Mutation

    gfp with first 10 leucine codons mutated to amber

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSUPER-retro-puro-shNup133-2

    Plasmid

    #87333

    Purpose

    To express shRNA against human Nup133

    Depositor

    Lei Lu

    Article

    Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

    Insert

    shRNA against human Nup133
    

    Use

    RNAi

    Expression

    Mammalian

    Promoter

    H1

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEPOR1CB0058

    Plasmid

    #117539

    Purpose

    Level1 Golden Gate Cassette: sgRNA cassette for SaCas9, targeting NbPDS gene in N. benthamiana

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    sgRNA (SaCas9)
    

    Expression

    Plant

    Available Since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLsCas13a-gRNA-1

    Plasmid

    #164865

    Purpose

    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for LsCas13a in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

    Insert

    LsCas13a-gRNA-1
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRSET FLIPE-100u

    Plasmid

    #13539

    Depositor

    Wolf Frommer

    Article

    Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

    Insert

    FLIP-E S118W
    

    Tags

    ECFP and Venus

    Expression

    Bacterial

    Mutation

    S118W in binding protein

    Available Since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBT374_pattB-pCA-GFP-pA-attBSwa

    Plasmid

    #52553

    Purpose

    for PhiC31 integrase-mediated cassette exchange

    Depositor

    Liqun Luo

    Article

    Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

    Inserts

    PhiC31o attB (full length)
    GFP
    

    Expression

    Mammalian

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. EGFP-bSV-1-830

    Plasmid

    #40963

    Depositor

    Elizabeth Luna

    Article

    Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

    Insert

    supervillin (SVIL Bovine)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    amino acids 1-830

    Available Since

    Dec. 12, 2012

    Availability

    Academic Institutions and Nonprofits only

25. knob-s48i48

    Plasmid

    #68391

    Purpose

    Knob protein fused to elastin-like polypeptide (S48I48)

    Depositor

    Andrew MacKay

    Article

    Sun et al J Control Release. 2011 Oct 30;155(2):21

    Insert

    Knob-S48I48
    

    Tags

    Knob

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pPS832

    Plasmid

    #1357

    Depositor

    Pamela Silver

    Article

    Schlenstedt et al Proc Natl Acad Sci U S A 1995 Jan 3;92(1

    Insert

    GSP1 (GSP1 Budding Yeast)
    

    Expression

    Yeast

    Available Since

    Aug. 3, 2005

    Availability

    Academic Institutions and Nonprofits only

27. pGEXndoE(E662Q)

    Plasmid

    #47716

    Purpose

    Expresses GST-EndoE(E662Q) from E. faecalis in E. coli

    Depositor

    Mattias Collin, Vincent Fischetti

    Article

    Collin et al J Biol Chem. 2004 May 21;279(21):22558-7

    Insert

    ndoE
    

    Tags

    GST-EndoE(E186Q) and Glutathione S-transferase

    Expression

    Bacterial

    Mutation

    Changed Glu-662 to Gln

    Promoter

    tac

    Available Since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pRS423-SgH

    Plasmid

    #163972

    Purpose

    Helper plasmid for cloning gRNAs under the control of the SNR52 promoter.

    Depositor

    Jiazhang Lian

    Article

    Dong et al ACS Synth Biol. 2020 Sep 18;9(9):2252-22

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Yeast

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Hy_pPepck1 eGFP SV40

    Plasmid

    #132644

    Purpose

    Expresses eGFP mRNA from the Drosophila Pepck1 promoter

    Depositor

    Jeremy Wilusz

    Article

    Tatomer et al Genes Dev. 2019 Sep 17. pii: gad.330167.

    Insert

    eGFP
    

    Expression

    Insect

    Promoter

    Pepck1

    Available Since

    Oct. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pc3XB-ZF5

    Plasmid

    #13104

    Depositor

    Keith Joung

    Article

    Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

    Insert

    ZF5
    

    Expression

    Mammalian

    Available Since

    Feb. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pENTR Bmi1-C

    Plasmid

    #30511

    Depositor

    John Gurdon

    Article

    Pasque et al EMBO J. 2011 May 6;30(12):2373-87. doi:

    Insert

    Bmi1 (Bmi1 Mouse)
    

    Use

    Gateway - donor vector

    Mutation

    NO STOP

    Available Since

    June 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAAVS1-NEO-CAG-BFP-pA-GFP-pA

    Plasmid

    #149344

    Purpose

    Vector for targeting the Replace reporter to the AAVS1 locus

    Depositor

    Ralf Kuehn

    Article

    Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

    Insert

    BFP
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA-HIRAmut-polyA

    Plasmid

    #59782

    Purpose

    This Hira gene contains silent mutations in siRNA-targeted sequences (see Inoue and Zhang, 2014 NSMB).

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    HIRA
    

    Expression

    Mammalian

    Mutation

    Change from 'GATCGAAGTTTG' to 'GAc…

    Promoter

    CMV and T7

    Available Since

    Sept. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pc3XB-ZF10

    Plasmid

    #13109

    Depositor

    Keith Joung

    Article

    Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

    Insert

    ZF10
    

    Expression

    Mammalian

    Available Since

    Feb. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pTEI134

    Plasmid

    #126071

    Purpose

    Vector for expression of plastid targeted YFP1-10 (T203Y) in plant cell

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Insert

    Chloroplastic FNR transit peptide
    

    Use

    Binary

    Tags

    YFP1-10

    Expression

    Plant

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pEPOR0SP0007

    Plasmid

    #117534

    Purpose

    Level0 Golden Gate part: CDS, LbCas12a

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    LbCas12a (with stop codon)
    

    Use

    CRISPR

    Available Since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pBSKΔB-CBh-Cas9n-pA

    Plasmid

    #82617

    Purpose

    Expresses Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

    Insert

    SpCas9n
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pc3XB-ZF64

    Plasmid

    #13200

    Depositor

    Daniel Voytas

    Article

    Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

    Insert

    ZF64
    

    Expression

    Mammalian

    Available Since

    Oct. 31, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pc3XB-ZF63

    Plasmid

    #13199

    Depositor

    Daniel Voytas

    Article

    Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

    Insert

    ZF63
    

    Expression

    Mammalian

    Available Since

    Oct. 31, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Hy_pMtnA nLuc SV40

    Plasmid

    #132654

    Purpose

    Expresses nanoLuciferase (nLuc) mRNA from the Drosophila Metallothionein A promoter

    Depositor

    Jeremy Wilusz

    Article

    Tatomer et al Genes Dev. 2019 Sep 17. pii: gad.330167.

    Insert

    nanoLuciferase
    

    Tags

    3xFLAG

    Expression

    Insect

    Promoter

    MtnA

    Available Since

    Oct. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pLY103

    Plasmid

    #130937

    Purpose

    Engineered sgRNA-LEA1 (2 BoxB aptamers) generator circuit including promoter Plux2

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Insert

    sgRNA-LEA1
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    Plux2

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pc3XB-ZF2

    Plasmid

    #13101

    Depositor

    Keith Joung

    Article

    Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

    Insert

    ZF2
    

    Expression

    Mammalian

    Available Since

    Feb. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pc3XB-ZF47

    Plasmid

    #13146

    Depositor

    Keith Joung

    Article

    Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

    Insert

    ZF47
    

    Expression

    Mammalian

    Available Since

    Feb. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pc3XB-ZF60

    Plasmid

    #13196

    Depositor

    Daniel Voytas

    Article

    Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

    Insert

    ZF60
    

    Expression

    Mammalian

    Available Since

    Oct. 31, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pEPOR0SP0021

    Plasmid

    #117532

    Purpose

    Level0 Golden Gate part: CDS, SaCas9 (no stop codon)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    SaCas9 (no stop codon)
    

    Use

    CRISPR

    Available Since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pT7ddx19gRNA

    Plasmid

    #47932

    Purpose

    in vitro transcription of ddx19 gRNA

    Depositor

    Wenbiao Chen, Susan Wente

    Article

    Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

    Insert

    ddx19 gRNA (ddx19 Zebrafish)
    

    Use

    CRISPR

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. PSUPER-344s(hPot1)

    Plasmid

    #11134

    Depositor

    Christopher Counter

    Article

    Veldman et al Curr Biol. 2004 Dec 29. 14(24):2264-70.

    Insert

    RNAi vector for human protection of telomeres 1(control for 344) (POT1 Human)
    

    Use

    RNAi

    Expression

    Mammalian

    Mutation

    N/A

    Available Since

    March 3, 2006

    Availability

    Academic Institutions and Nonprofits only

48. pc3XB-ZF72

    Plasmid

    #13206

    Depositor

    Daniel Voytas

    Article

    Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

    Insert

    ZF72
    

    Expression

    Mammalian

    Available Since

    Oct. 31, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pET15b-YC

    Plasmid

    #107072

    Purpose

    Host vector to construct a YPer or CyPet fusion protein

    Depositor

    Teruyuki Nagamune

    Article

    Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

    Insert

    YPet-CyPet fusion protein
    

    Tags

    His6 tag

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. Hy_pMT dati MCS circfirefly_1677

    Plasmid

    #110118

    Purpose

    Expresses a 1677-nt firefly luciferase circular RNA in Drosophila cells

    Depositor

    Jeremy Wilusz

    Article

    Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

    Insert

    Firefly luciferase
    

    Expression

    Insect

    Promoter

    Metallothionein Promoter (pMT)

    Available Since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge