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We narrowed to 14,447 results for: cas9 genes

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Showing: 2841 - 2880 of 14447 results

  1. pCW-Tet-Cas9-miRFP670nano-PGK-Blast

    Plasmid
    #196722
    Purpose
    Tet-inducible expression of Cas9-T2A-miRFP670nano
    Depositor
    Bernhard Schmierer
    Insert
    Cas9-T2A-miRFP670nano
    Use
    CRISPR and Lentiviral
    Tags
    3xFLAG-SV40NLS and Nucleoplasmin NLS
    Promoter
    TRE, PGK
    Available Since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196722-plasmid-map-sequence-id-389804

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  2. PB-CAG-DDdCas9VPP300-T2A-GFP-IRES-Neo

    Plasmid
    #102887
    Purpose
    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-P300core activator followed by T2A-EGFP as a reporter and IRES-Neo as selection
    Depositor
    Timo Otonkoski
    Article
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Insert
    DDdCas9VP192-P300-T2A-GFP-IRES-Neo
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CAG
    Available Since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102887-plasmid-map-sequence-id-262467

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  3. PB-CAG-DDdCas9VPPH-T2A-GFP-IRES-Neo

    Plasmid
    #102888
    Purpose
    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-P300core-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection
    Depositor
    Timo Otonkoski
    Article
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Insert
    DDdCas9VP192-p65-HSF1-P300-T2A-GFP-IRES-Neo
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CAG
    Available Since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102888-plasmid-map-sequence-id-262466

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  7. pLenti-U6-sgCTG-CAG-Cas9D10A nickase-Blast

    Plasmid
    #216733
    Purpose
    Expresses SpCas9-D10A nickase under a CAG promoter together with a blasticidin resistance gene, along with a U6 promoter driving the sgCTG (target sequence: (CUG)6). Suitable for lentivirus packaging.
    Depositor
    Vincent Dion
    Article
    Alvaro et al bioRxiv
    Insert
    Cas9-D10A nickase and sgCTG
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    U6 and CAG
    Available Since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216733-plasmid-map-sequence-id-430318

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  8. pU6-(BsaI)_CBh-UC-Cas9-T2A-mCherry

    Plasmid
    #135014
    Purpose
    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to C-terminus of Cas9, linked to mCherry via a T2A peptide
    Depositor
    Yosef Shaul
    Article
    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio
    Insert
    humanized S. pyogenes Cas9
    Tags
    3x Flag, 3xFLAG (N terminal on insert), NLS, and …
    Expression
    Mammalian
    Mutation
    126aa domain from HSV-1 UL12 fused to the C-termi…
    Promoter
    CBh
    Available Since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    135014-plasmid-map-sequence-id-267599

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  13. pU6-(BsaI)_CBh-UN-Cas9-T2A-mCherry

    Plasmid
    #135013
    Purpose
    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to N-terminus of Cas9, linked to mCherry via a T2A peptide
    Depositor
    Yosef Shaul
    Article
    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio
    Insert
    humanized S. pyogenes Cas9
    Tags
    3x Flag, 3xFLAG (N terminal on insert), NLS, and …
    Expression
    Mammalian
    Mutation
    126aa domain from HSV-1 UL12 fused to the N-termi…
    Promoter
    CBh
    Available Since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    135013-plasmid-map-sequence-id-267601

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  15. BPK3258 - human expression plasmid for eSpCas9(1.1)

    Plasmid
    #101176
    Purpose
    Human expression plasmid for SpCas9 eSpCas9(1.1) variant: CMV-T7-hSpCas9-eSpCas9(1.1)(K848A, K1003A, R1060A)-NLS(SV40)-3xFLAG
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    hSpCas9-eSpCas9(1.1)(K848A/K1003A/R1060A)
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    K848A/K1003A/R1060A
    Promoter
    CMV
    Available Since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101176-plasmid-map-sequence-id-193999

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  22. PB-tight-DDdCas9VPPH-T2A-GFP-IRES-Neo

    Plasmid
    #102892
    Purpose
    PiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-P300core-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection
    Depositor
    Timo Otonkoski
    Article
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Insert
    DDdCas9VP192-p65-HSF1-P300-T2A-GFP-IRES-Neo
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    TRE-tight
    Available Since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102892-plasmid-map-sequence-id-198257

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  23. pU6-SacB-Cas9-T2A-mCherry_(BbsI)

    Plasmid
    #117070
    Purpose
    Encodes Cas9, T2A-mCherry, and AmpR which creates a CRISPR system application with reliable positive selection
    Depositor
    Kim Failor
    Inserts
    Cas9
    SacB
    Use
    CRISPR
    Tags
    3x FLAG, NLS, and T2A-mCherry
    Expression
    Mammalian
    Promoter
    CBh and U6
    Available Since
    Dec. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    117070-plasmid-map-sequence-id-231696

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  29. PB-tight-DDdCas9VPP300-T2A-GFP-IRES-Neo

    Plasmid
    #102891
    Purpose
    PiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-P300core activator followed by T2A-EGFP as a reporter and IRES-Neo as selection
    Depositor
    Timo Otonkoski
    Article
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Insert
    DDdCas9VP192-P300-T2A-GFP-IRES-Neo
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    TRE-tight
    Available Since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102891-plasmid-map-sequence-id-198253

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  31. A3Bi-ctd E255A -Cas9n-UGI-NLS

    Plasmid
    #109429
    Purpose
    Expresses the C-terminal catalytically inactive mutant of human APOBEC3B containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.
    Depositor
    Reuben Harris
    Article
    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994
    Insert
    Apolipoprotein mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal Doman E255A Catalytic Mutant (APOBEC3B Human)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Insertion of an L1 intron into the coding sequenc…
    Promoter
    CMV
    Available Since
    July 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    109429-plasmid-map-sequence-id-212934

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  39. pDGB3 alpha1 Tnos:nptII:Pnos - SF - P35S:hCas9:Tnos (GB1656)

    Plasmid
    #160618
    Purpose
    Module for the expression of the human codon optimized Cas9 and kanamycin resistance (NptII).
    Depositor
    Diego Orzaez
    Insert
    Tnos:NptII:Pnos-SF-P35s:hCas9:tNos
    Use
    CRISPR
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Pnos, 35S
    Available Since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    160618-plasmid-map-sequence-id-322016

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  40. dCas9-3×RFP-P2A-CASANOVA/gRNA telo

    Plasmid
    #137711
    Purpose
    Vector co-expressing dCas9-3×RFP-P2A-CASANOVA and sgRNA telo
    Depositor
    Dominik Niopek
    Insert
    dCas9-3×RFP-P2A-CASANOVA/gRNA telo
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    137711-plasmid-map-sequence-id-273733

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Showing: 2841 - 2880 of 14447 results