We narrowed to 2,452 results for: LRE

1. AIP(WT)-mChF-giantin

   Plasmid

   #61523

   Purpose

   Expression of human golbin B1 and FK506 binding protein 1A (FKBP1A) tagged with mCherry and AIP

   Depositor

   Takanari Inoue

   Article

   Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

   Inserts

   FK506 binding protein 1A (FKBP1A Human)
   golgin B1 (GOLGB1 Human)
   

   Use

   Tags

   AIP and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 17, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pKLV2-U6gRNA3(BbsI)-PGKpuro2ABFP

   Plasmid

   #67990

   Purpose

   CRISPR gRNA expression vector with the conventional scaffold and puro/BFP markers without WPRE

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette with the conventional scaffold, PGKpuro2ABFP cassette
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-Htt171-18Q-myc-WPRE

   Plasmid

   #107936

   Purpose

   Adeno-Associated viral (AAV) vector plasmid expressing exon1 of wild type HTT (wtHTT, 18 polyQ repeats) in neurons

   Depositor

   Johan Jakobsson

   Article

   Pircs et al Cell Rep. 2018 Aug 7;24(6):1397-1406. do

   Insert

   Exon 1 of wild type HTT (HTT Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSynapsin

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAS_4xhyb PylT A41AA C55A CRFR1 95TAA 263TAG-HA

   Plasmid

   #154780

   Purpose

   plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and rat CRFR1 95TAA 263TAG, for transient transfection or stable, piggybac-mediated, integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

   Insert

   CRFR1 (Crhr1 Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   95TAA 263TAG in CRFR1-HA, hybrid PylT with A41AA …

   Promoter

   EF1

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKLV2-U6gRNA5(gBFP)-PGKGFP2ABFP-W

   Plasmid

   #67984

   Purpose

   Cas9 activity reporter with GFP and BFP

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Inserts

   U6gRNA cassette, PGKGFP2ABFP cassette, WPRE
   Guide RNA targeting modified BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Deleted BbsI site within WPRE, modified BFP

   Promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLL1 sgRNA(MS2)-MS2-AIDmono

   Plasmid

   #112127

   Purpose

   empty sgRNA cloning vector with MS2-AIDmono

   Depositor

   Fei-Long Meng

   Article

   Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

   Insert

   AID mono mutant (AICDA Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   N- mutated and C- terminal truncated, H130A/R131E…

   Promoter

   hU6,CBh

   Available since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYX-N-Puro-mNeon-WNK3

   Plasmid

   #222674

   Purpose

   endogenously tag WNK3 with N-terminal mNeon

   Depositor

   Jason Moffat

   Article

   Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

   Insert

   WNK3 (WNK3 Human)
   

   Use

   Tags

   mNeon

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAD-CMV-Caveolin1-CMV-GFP

   Plasmid

   #83272

   Purpose

   Adenoviral expression of Caveolin1 with co-expression of GFP

   Depositor

   Andrew Brooks

   Article

   Fernandez-Rojo et al Cell Rep. 2013 Jul 25;4(2):238-47. doi:

   Inserts

   CAV1 (CAV1 Human)
   eGFP
   

   Use

   Adenoviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   July 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-H2B-iRFP670-p2a-YFP-Rb-IRES-blasticidin

   Plasmid

   #223962

   Purpose

   Dual fluorescent reporter for histone H2B and for exogenous Rb

   Depositor

   Hee Won Yang

   Article

   Kim et al Cell Rep. 2023 Nov 28;42(11):113198. doi

   Insert

   H2B-iRFP670-p2a-YFP-Rb (RB1, H2B Human)
   

   Use

   Tags

   YFP and iRFP670

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR 223 VPS4B

    Plasmid

    #169320

    Purpose

    Gateway entry vector for human VPS4B ORF

    Depositor

    Francisca Vazquez

    Article

    Neggers et al Cell Rep. 2020 Dec 15;33(11):108493. doi

    Insert

    VPS4B (VPS4B Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    NA

    Available since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTR223-CMV-Cre-U6-sgX-U6-sgRb1-U6-sgTrp53-U6-sgRbl2

    Plasmid

    #209060

    Purpose

    Entry cloning vector to insert an sgRNA of interest (using Esp3i digestion) into a vector that already contains sgRNAs against mouse Rb1, Trp53, and Rbl2 and CMV Cre recombinase.

    Depositor

    Matthew Oser

    Article

    Duplaquet et al Nat Cell Biol. 2023 Sep;25(9):1346-1358.

    Insert

    sgRNAs targeting Rb1, Trp53, Rbl2 and Cre recombinase
    

    Use

    Gateway vector to be used for lr reaction

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Nov. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLL3 sgRNA(MS2)-MS2-AID3f

    Plasmid

    #112129

    Purpose

    empty sgRNA cloning vector with MS2-AID3f

    Depositor

    Fei-Long Meng

    Article

    Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

    Insert

    AIDmono 3f mutant (AICDA Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    N- mutated and C- terminal truncated, F115Y/C116F…

    Promoter

    hU6,CBh

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLL2 sgRNA(MS2)-MS2-AID3c

    Plasmid

    #112128

    Purpose

    empty sgRNA cloning vector with MS2-AID3c

    Depositor

    Fei-Long Meng

    Article

    Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

    Insert

    AID mono 3c mutant (AICDA Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    N- mutated and C- terminal truncated, F115Y/C116F…

    Promoter

    hU6,CBh

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pYX-N-Puro-mNeon-TSC22D4

    Plasmid

    #222671

    Purpose

    endogenously tag TSC22D4 with N-terminal mNeon

    Depositor

    Jason Moffat

    Article

    Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

    Insert

    TSC22D4 (TSC22D4 Human)
    

    Use

    Tags

    mNeon

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLenti-CMV-Blast-DNFGFR1-HA

    Plasmid

    #130887

    Purpose

    Expresses dominant negative mutant mouse FGFR1

    Depositor

    Gian-Paolo Dotto

    Article

    Bordignon et al Cell Rep. 2019 Aug 27;28(9):2358-2372.e6

    Insert

    Fgfr1 (Fgfr1 Mouse)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    Dominant Negative receptor lacking cytosolic cata…

    Promoter

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV8-hSyn-flex-miR30-eGFP-shCrh

    Plasmid

    #132715

    Purpose

    Encodes Cre-dependent short hairpin RNA (shRNA) that targets the 3’-untranslated region of the rat Crh gene which encodes corticotropin releasing factor

    Depositor

    Robert Messing

    Article

    Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

    Insert

    GFP-shCrh (Crh Rat)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Synapsin 1

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. peGFP-N1-Cofilin

    Plasmid

    #186747

    Purpose

    Expresses wildtype GFP-tagged human cofilin in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

    Insert

    Cofilin-1 (CFL1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pKLV2-U6gRNA5(Empty)-PGKGFP2ABFP-W

    Plasmid

    #67983

    Purpose

    Cas9 activity reporter (control) with GFP and BFP

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKGFP2ABFP cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE, modified BFP

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCW57-mCherry-2A-BRD4 Iso AdelCTD

    Plasmid

    #137722

    Purpose

    Doxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 long isoform missing its C-terminal, P-TEFb interacting domain (CTD)

    Depositor

    Scott Floyd

    Article

    Edwards et al Cell Rep. 2020 Sep 22;32(12):108166. doi

    Insert

    BRD4 long isoform without its C-terminal, P-TEFb interacting domain (BRD4 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Truncated at amino acid 1328

    Promoter

    Tight TRE promoter

    Available since

    Jan. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GST-FUS 465-526

    Plasmid

    #44983

    Depositor

    Robin Reed

    Article

    Yamazaki et al Cell Rep. 2012 Oct 25;2(4):799-806. doi:

    Insert

    FUS (465-526aa) (FUS Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    contains amino acids 465-526

    Promoter

    tac

    Available since

    June 26, 2013

    Availability

    Academic Institutions and Nonprofits only

21. MSCV-ASNS-OE-IRES-Thy1.1

    Plasmid

    #192947

    Purpose

    Asparagine synthetase overexpression in murine cells

    Depositor

    Ping-Chih Ho

    Article

    Fernandez-Garcia et al Cell Rep. 2022 Nov 15;41(7):111639. doi:

    Insert

    Asparagine synthetase (Asns Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    5'LTR

    Available since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. GST-FUS 266-526

    Plasmid

    #44981

    Depositor

    Robin Reed

    Article

    Yamazaki et al Cell Rep. 2012 Oct 25;2(4):799-806. doi:

    Insert

    FUS (266-526aa) (FUS Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    contains amino acids 266-526

    Promoter

    tac

    Available since

    June 26, 2013

    Availability

    Academic Institutions and Nonprofits only

23. GST-FUS 356-526

    Plasmid

    #44982

    Depositor

    Robin Reed

    Article

    Yamazaki et al Cell Rep. 2012 Oct 25;2(4):799-806. doi:

    Insert

    FUS (356-526aa) (FUS Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    contains amino acids 356-526

    Promoter

    tac

    Available since

    June 3, 2013

    Availability

    Academic Institutions and Nonprofits only

24. MIGR1-Cdc42-1

    Plasmid

    #179517

    Purpose

    Retroviral expression of Cdc42 splice variant 1

    Depositor

    Bo Porse

    Article

    Rehn et al Cell Rep. 2022 May 10;39(6):110793. doi:

    Insert

    Cdc42 splice variant 1 cDNA (CDC42 Human)
    

    Use

    Retroviral

    Tags

    IRES-EGFP

    Expression

    Mutation

    Promoter

    LTR

    Available since

    April 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Cav2.1 HA EI,II,III,IVA (E320A, E670A, E1411A, E1707A) pCAGGS

    Plasmid

    #206115

    Purpose

    expression of rat Cav2.1 with an exofacial double HA tag in domain II and mutations in the P loop of Domains I, II, III and IV which abolish trafficking to the cell surface

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1b (Cacna1a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E320A, E670A, E1411A, E1707A

    Promoter

    CMV/B-actin

    Available since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Cav2.1 HA EI,II,III,IVA (E320A, E670A, E1411A, E1707A) pcDNA3

    Plasmid

    #206121

    Purpose

    expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domains I, II, III and IV to to abolish divalent cation binding

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1a (Cacna1a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E320A, E670A, E1411A, E1707A

    Promoter

    CMV

    Available since

    Oct. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. MIGR1-Cdc42-2

    Plasmid

    #179518

    Purpose

    Retroviral expression of Cdc42 splice variant 2

    Depositor

    Bo Porse

    Article

    Rehn et al Cell Rep. 2022 May 10;39(6):110793. doi:

    Insert

    Cdc42 splice variant 2 cDNA (CDC42 Human)
    

    Use

    Retroviral

    Tags

    IRES-EGFP

    Expression

    Mutation

    Promoter

    LTR

    Available since

    April 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pIGF1_P2 Promoter-FLuc

    Plasmid

    #154267

    Purpose

    Firefly luciferase reporter containing the downstream Human IGF-1 P2 Promoter.

    Depositor

    Antonio Amelio

    Article

    Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

    Insert

    IGF-1 P2 Promoter (IGF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human IGF-1 P2 Promoter

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. TMEM230-WT-IRES(isoform 2)

    Plasmid

    #99564

    Purpose

    mammalian expression of TMEM230 (isoform 2) and ZsGreen

    Depositor

    Han-Xiang Deng

    Article

    Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

    Insert

    TMEM230 (TMEM230 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMIG-AVITEV- hNFATc1/aA

    Plasmid

    #74057

    Purpose

    retroviral expression plasmid for human NFATc1/aA with N-terminal BirA-biotinylation signal and TEV celavage site

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    human NFATc1, isoform alpha-A (NFATC1 Human)
    

    Use

    Retroviral

    Tags

    AVI-TEV

    Expression

    Mammalian

    Mutation

    mutation G1157A [R235Q] and the silent mutation C…

    Promoter

    pMSCV-LTRs

    Available since

    June 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge