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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 33,294 results for: Nes

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Showing: 2341 - 2370 of 33294 results

  3. PB-SAM

    Plasmid
    #102559
    Purpose
    Piggybac transposon vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex.
    Depositor
    Ying Liu
    Article
    Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64
    Inserts
    MS2-P65-HSF1_T2A_Hygro
    dCAS9(D10A, N863A)-VP64_T2A_Blast
    Use
    CRISPR; Piggybac transposon
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9 and N55K in MS2
    Promoter
    CAG and EF1A
    Available Since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    102559-plasmid-map-sequence-id-193885

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  7. pMJ922

    Plasmid
    #78312
    Purpose
    Expression of His6-MBP-tagged Cas9-NLS-EGFP protein in E. coli
    Depositor
    Martin Jinek
    Article
    Burger et al Development. 2016 Apr 29. pii: dev.13480
    Insert
    SpCas9
    Tags
    HA-2xNLS-EGFP-NLS and His6-MBP-TEV
    Expression
    Bacterial
    Mutation
    human codon-optimized gene sequence
    Promoter
    T7
    Available Since
    June 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    78312-plasmid-map-sequence-id-457381

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  10. pEC3109 (pSpy1C-Plac(1)_ffluc)

    Plasmid
    #218514
    Purpose
    replicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressor
    Depositor
    Emmanuelle Charpentier
    Article
    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1
    Insert
    ffluc
    Use
    Luciferase
    Expression
    Bacterial
    Mutation
    deletion of mrfp cassette, mutagenesis of the RBS…
    Promoter
    Plac(1)
    Available Since
    Sept. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    218514-plasmid-map-sequence-id-444100

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  12. pMi[3xP3::mCherry-SV40, Plodia-FibL::mBaojin-P10]

    Plasmid
    #240410
    Purpose
    For Minos transgenesis in insect, expresses the 3xP3::mCherry eye/glia transgenesis, and the mBaoJin in Posterior Silk Glands (under the control of the Fibroin-L promoter from Plodia interpunctella)
    Depositor
    Arnaud Martin
    Article
    Shodja et al PeerJ. 2025 Nov 12;13:e20249. doi: 10.77
    Inserts
    3xP3::mCherry_SV40terminator
    FibL::mBaojin_P10terminator (Fib-l Synthetic)
    Expression
    Insect
    Promoter
    3xP3 and Fibroin-L proximal promoter (Plodia inte…
    Available Since
    Aug. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    240410-plasmid-map-sequence-id-483538

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  20. pMJ923

    Plasmid
    #78313
    Purpose
    Expression of His6-MBP-tagged Cas9-NLS-mCherry protein in E. coli
    Depositor
    Martin Jinek
    Article
    Burger et al Development. 2016 Apr 29. pii: dev.13480
    Insert
    SpCas9
    Tags
    HA-2xNLS-mCherry-NLS and His6-MBP-TEV
    Expression
    Bacterial
    Mutation
    human codon-optimized gene sequence
    Promoter
    T7
    Available Since
    June 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    78313-plasmid-map-sequence-id-148391

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  23. pcDNA3-Lyso-mCherry-Rheb(C181S)

    Plasmid
    #192438
    Purpose
    Encodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant targeted to lysosome membrane.
    Depositor
    Jin Zhang
    Article
    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1
    Insert
    Lyso-mCherry-Rheb(C181S) (RHEB Human)
    Tags
    Full-length LAMP1 and mCherry
    Expression
    Mammalian
    Mutation
    Rheb Cys 181 mutated to Ser; abolishes C-terminal…
    Promoter
    CMV
    Available Since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    192438-plasmid-map-sequence-id-379134

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  25. pEC3111 (pSpy1C-Plac(3)_ffluc)

    Plasmid
    #218778
    Purpose
    replicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressor
    Depositor
    Emmanuelle Charpentier
    Article
    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1
    Insert
    ffluc
    Use
    Luciferase
    Expression
    Bacterial
    Mutation
    deletion of mrfp cassette, mutagenesis of the RBS…
    Promoter
    Plac(3)
    Available Since
    Sept. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    218778-plasmid-map-sequence-id-444099

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  27. 3xFLAG-dCas9/MSCV-EGFP

    Plasmid
    #82613
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Fujita et al Genes Cells. 2018 Feb 26. doi: 10.1111/g
    Insert
    3xFLAG-dCas9
    Use
    Retroviral
    Tags
    3xFLAG tag and NLS (nuclear localization signal)
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available Since
    Sept. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    82613-plasmid-map-sequence-id-298740

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Showing: 2341 - 2370 of 33294 results