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We narrowed to 5,579 results for: PEP

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Showing: 3901 - 3950 of 5579 results

  2. Avr2-GFP pZK538

    Plasmid
    #118011
    Purpose
    expresses Avr2-GFP (without signal peptide) and mCherry-HDEL. Used for visualization of cell-to-cell movement of Avr2. Avr2 can be replaced with gene of interest via HiFi DNA assembly cloning (NEB) .
    Depositor
    FLW Takken
    Article
    et al Bio-protocol 8(24): e3114.
    Inserts
    dspAvr2
    mCherry
    Tags
    EGFP and HDEL
    Expression
    Bacterial and Plant
    Promoter
    35S
    Available Since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    118011-plasmid-map-sequence-id-307579

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  4. mApple-hCOL1A1

    Plasmid
    #140113
    Purpose
    Expresses fluorescently-tagged human Type I procollagen α1 chain with mApple replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site
    Depositor
    Sergey Leikin
    Insert
    Type I procollagen α1 (COL1A1 Human)
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    COL1A1 exons 2-3 replaced by fluorescent tag
    Promoter
    CMV
    Available Since
    May 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    140113-plasmid-map-sequence-id-296772

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  5. mVenus-hCOL1A1

    Plasmid
    #140111
    Purpose
    Expresses fluorescently-tagged human Type I procollagen α1 chain with mVenus replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site
    Depositor
    Sergey Leikin
    Insert
    Type I procollagen α1 (COL1A1 Human)
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    COL1A1 exons 2-3 replaced by fluorescent tag
    Promoter
    CMV
    Available Since
    May 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    140111-plasmid-map-sequence-id-296751

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  9. mCerulean-hCOL1A1

    Plasmid
    #140112
    Purpose
    Expresses fluorescently-tagged human Type I procollagen α1 chain with mCerulean replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site
    Depositor
    Sergey Leikin
    Insert
    Type I procollagen α1 (COL1A1 Human)
    Tags
    mCerulean
    Expression
    Mammalian
    Mutation
    COL1A1 exons 2-3 replaced by fluorescent tag
    Promoter
    CMV
    Available Since
    May 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    140112-plasmid-map-sequence-id-296778

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  11. pOCC154

    Plasmid
    #118902
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal TwinStrep, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-NotI-ccdB-AscI-3C-TwinStrep-stop-HindIII cassette
    Tags
    TwinStrep, cleavable with 3C protease
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118902-plasmid-map-sequence-id-232325

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  13. pOCC7

    Plasmid
    #118876
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal HIS6 tag, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
    Tags
    HIS6, cleavable with 3C protease
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118876-plasmid-map-sequence-id-232237

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  16. pOCC153

    Plasmid
    #118901
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal TwinStrep, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-TwinStrep-3C-NotI-ccdB-AscI-stop-HindIII cassette
    Tags
    TwinStrep, cleavable with 3C protease
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118901-plasmid-map-sequence-id-232312

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  24. pNAS1B split mCherry N-ME(pS)VD/TRAP(RK)-C

    Plasmid
    #61966
    Purpose
    Positive control duet plasmid for SepOTS-dependent split mCherry assembly assay (i.e., N-mCherry is fused to the peptide ME(pS)VD (pS = phosphoserine); C-mCherry is fused to TRAP(RK))
    Depositor
    Lynne Regan
    Article
    Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.
    Inserts
    N-mCherry-ME(pS)VD
    TRAP(RK)-C-mCherry
    Expression
    Bacterial
    Promoter
    PBAD and PLtetO
    Available Since
    Feb. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    61966-plasmid-map-sequence-id-101265

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  28. MX01

    Plasmid
    #158624
    Purpose
    GATEWAY and BacMam compatible mammalian expression vector with eGFP expression reporter (eGFP is expressed by same promotor but as a separate polypeptide chain).
    Depositor
    Timm Maier
    Article
    Scaiola et al Sci Adv. 2020 Nov 6;6(45). pii: 6/45/eab
    Type
    Empty backbone
    Use
    Gateway destination vector ; bacmam acceptor vect…
    Tags
    P2A
    Expression
    Mammalian
    Promoter
    CMV-IE
    Available Since
    Nov. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    158624-plasmid-map-sequence-id-310153

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  29. pSCM167.2-Venus-VF-hADAM10(aa1-213)

    Plasmid
    #189554
    Purpose
    Protein expression ADAM10 in mammalian cells. Contains only prodomain--amino acids 1-213
    Depositor
    Haian Fu
    Insert
    truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
    Tags
    VF
    Expression
    Mammalian
    Mutation
    contains aa 1-213
    Available Since
    Feb. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    189554-plasmid-map-sequence-id-384131

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  38. HA-hDAGLb-V5

    Plasmid
    #87675
    Purpose
    Expresses human DAGLb protein with a HA tag inserted in the first extracellular loop, and intracellular V5 tag located on the C-terminal
    Depositor
    Pat Doherty
    Insert
    Human Diacylglycerol Lipase Beta (DAGLB Human)
    Tags
    HA and V5 tag
    Expression
    Mammalian
    Mutation
    Hemagglutinin (HA) peptide sequence (YPYDVPDYA) f…
    Promoter
    T7
    Available Since
    March 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    87675-plasmid-map-sequence-id-173605

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  40. pOCC114

    Plasmid
    #118904
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal sortase site
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-TEV-GG-NotI-ccdB-AscI-stop-HindIII cassette
    Tags
    HIS6, cleavable with TEV protease, two glycines
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118904-plasmid-map-sequence-id-232331

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  41. pMH-macroHC

    Plasmid
    #82337
    Purpose
    Provides a C-terminal macro domain and 6xHis tag for expression in E. coli
    Depositor
    Michael Hothorn
    Article
    Wild et al Protein Sci. 2016 Oct 24. doi: 10.1002/p
    Insert
    SPX domain of the Vacuolar Transporter Chaperone 4 (VTC4 Budding Yeast)
    Tags
    6xHis non-cleavable and macro domain of human his…
    Expression
    Bacterial
    Mutation
    Truncated protein, amino acids 1-178
    Available Since
    Nov. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    82337-plasmid-map-sequence-id-155830

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  48. Myog'-2A-mCherryNLS-PGK-Purodtk

    Plasmid
    #69547
    Purpose
    Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.
    Depositor
    Charles Gersbach
    Inserts
    2A-mCherry-NLS
    Myogenin 5' homology arm (Myog Mouse)
    Myogenin 3' homology arm (Myog Mouse)
    Puro-delta-tk
    Use
    Mouse Targeting
    Tags
    2x nuclear localization signal and PVT1-2A "…
    Mutation
    Silent V205V mutation in myogenin gene to prevent…
    Available Since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    69547-plasmid-map-sequence-id-122991

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  50. pET28a-HsSENP7

    Plasmid
    #71470
    Purpose
    Encodes human full length SENP7. Suitable for in vitro transcription/translation (T7).
    Depositor
    Jorge Eduardo Azevedo
    Article
    Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:
    Insert
    SUMO1/sentrin specific peptidase 7 (SENP7 Human)
    Tags
    Hexahistidine
    Expression
    Bacterial
    Mutation
    Gene optimised for E. coli expression (Genscript)
    Promoter
    T7
    Available Since
    Jan. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 3901 - 3950 of 5579 results