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We narrowed to 4,225 results for: pes

Showing: 2371 - 2400 of 4225 results

  1. pJET-osr1-KI-donor

    Plasmid
    #184875
    Purpose
    Donor template for knock-in of p2a-CreERT2 into the medaka osr1 locus via CRISPR/Cas9-mediated homology directed repair
    Depositor
    Christoph Winkler
    Article
    Hui et al Development. 2022 May 20. pii: 275483. d
    Inserts
    Odd-Skipped Related Transcription Factor 1 5' homology arm
    Odd-Skipped Related Transcription Factor 1 3' homology arm
    p2a-CreERT2
    Myosin light chain 2 promoter
    EGFP
    Use
    CRISPR and Cre/Lox
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    184875-plasmid-map-sequence-id-366330

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  2. pAAV-hSyn-mCherry.3xFLAG.NOS1AP396-503-WPRE

    Plasmid
    #174133
    Purpose
    pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP396-503 fusion protein under the hSyn promoter
    Depositor
    Florian Freudenberg
    Article
    Freudenberg et al EBioMedicine. 2021 Aug 26;71:103565. doi
    Insert
    Nos1ap (Nos1ap Mouse)
    Use
    AAV
    Tags
    3xFLAG and mCherry
    Expression
    Mutation
    Only contains the sequences coding for amino acid…
    Promoter
    hSyn
    Available since
    Oct. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    174133-plasmid-map-sequence-id-345689

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  3. pAAV-hSyn-mCherry.3xFLAG.NOS1AP396-427-WPRE

    Plasmid
    #174135
    Purpose
    pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP396-427 fusion protein under the hSyn promoter
    Depositor
    Florian Freudenberg
    Article
    Freudenberg et al EBioMedicine. 2021 Aug 26;71:103565. doi
    Insert
    Nos1ap (Nos1ap Mouse)
    Use
    AAV
    Tags
    3xFLAG and mCherry
    Expression
    Mutation
    Only contains the sequences coding for amino acid…
    Promoter
    hSyn
    Available since
    Oct. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    174135-plasmid-map-sequence-id-345687

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  5. Progerin-S22D-CS-pLPC

    Plasmid
    #73810
    Purpose
    Progerin mutant where Serine 22 is mutated to aspartic acid and where there is a substitution of cysteine in CaaX-motif by a serine
    Depositor
    Gerardo Ferbeyre
    Article
    Moiseeva et al Aging (Albany NY). 2016 Feb;8(2):366-81.
    Insert
    Progerin (LMNA Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Progerin with substitution of cysteine in CaaX-mo…
    Promoter
    CMV
    Available since
    April 1, 2016
    Availability
    Academic Institutions and Nonprofits only

  6. pRS426-TUB1-internal6xHis - human TUBA1a Cterminal tail - C130S, E446C

    Plasmid
    #60394
    Purpose
    Expression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis with human alpha tubulin Cterminal tail (TUBA1a) C130S, E446C for crosslinking polyglutamate peptide
    Depositor
    Ron Vale
    Article
    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do
    Insert
    TUB1 (TUB1 Budding Yeast, Synthetic)
    Use
    Tags
    Expression
    Yeast
    Mutation
    internal 6xHis (see supplement figure 4), amino a…
    Promoter
    GAL
    Available since
    Jan. 26, 2015
    Availability
    Academic Institutions and Nonprofits only

  9. pDONR221-ETV3

    Plasmid
    #88804
    Purpose
    Donor Vector containing ETV3 transcription factor, part of the Human TFome Collection
    Depositor
    Marc Vidal
    Article
    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.
    Insert
    ETV3 (ETV3 Human)
    Use
    Gateway donor vector
    Tags
    Expression
    Mutation
    Last nucleotide of stop codon removed to allow fo…
    Promoter
    Available since
    June 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    88804-plasmid-map-sequence-id-410021

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  13. pAGT9054

    Plasmid
    #219430
    Purpose
    Transcriptional unit (MoClo-position 2): 2x35Ss_Omega enhancer_PapE-4LF2-N-SpCas9i-N_tOCS
    Depositor
    Tom Schreiber
    Article
    Schreiber et al Mol Plant. 2024 Mar 22:S1674-2052(24)000
    Insert
    LB_2x35Ss-omega enhancer:PapE-4xLF2-NLS-SpCas9i-NLS:tOCS_RB (Position 2)
    Use
    CRISPR; Moclo compatible level 1 module (position…
    Tags
    PapE-4LF2
    Expression
    Plant
    Mutation
    Promoter
    Available since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    219430-plasmid-map-sequence-id-441037

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  17. GABA(A) receptor subunit a2SE

    Plasmid
    #49169
    Purpose
    pHluorin-tagged GABA A receptor subunit (alpha 2) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface
    Depositor
    Tija Jacob, Stephen Moss
    Article
    Tretter et al J Neurosci. 2008 Feb 6;28(6):1356-65. do
    Insert
    GABA(A) receptor subunit alpha-2 (GABRA2 Human)
    Use
    Tags
    HA, bovine alpha 1 signal sequence, and pHGFP (pH…
    Expression
    Mammalian
    Mutation
    T343A
    Promoter
    CMV
    Available since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    49169-plasmid-map-sequence-id-72495

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  19. TFORF0351

    Plasmid
    #143694
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    FOXL2 (FOXL2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    143694-plasmid-map-sequence-id-404770

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  20. pCS6-ZF(VEGFA)-StaPL(AI)-YFP-VPR

    Plasmid
    #111502
    Purpose
    Expresses a zinc finger specific to the human VEGFA locus, which is linked to a VPR transcriptional activation domain via a StaPL(AI) module, in mammalian cells. [AI = asunaprevir inhibited].
    Depositor
    Michael Lin
    Article
    Jacobs et al Nat Methods. 2018 Jul;15(7):523-526. doi
    Insert
    ZF(VEGFA)-StaPL(AI)-YFP-VPR
    Use
    Tags
    Expression
    Mammalian
    Mutation
    The HCV NS3 protease carries V36M, T54A, and S122…
    Promoter
    CMV
    Available since
    July 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    111502-plasmid-map-sequence-id-214269

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  22. pTR-UF11

    Plasmid
    #157970
    Purpose
    AAV vector plasmid containing the Chimeric CMV/Chicken Beta Actin promoter driving 'humanized' GFP
    Depositor
    Sergei Zolotukhin
    Article
    Burger et al Mol Ther. 2004 Aug;10(2):302-17. doi: 10
    Has Service
    AAV.A01.T, AAV.A02.T, AAV.A03.T, AAV.A04.T, AAV.A05.T, AAV.A06.T, AAV.A07.T, AAV.A08.T, AAV.A09.T, AAV.A10.T, AAV.A11.T, and AAV.A12.T
    Insert
    'humanized' e.g. codon optimized GFP
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    chimeric CMV/Chicken Beta actin (CBA)
    Available since
    Oct. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    157970-plasmid-map-sequence-id-313812

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  24. 3'-ecRESCUE-mCherry

    Plasmid
    #191486
    Purpose
    ecDHFR DD-mCherry, an inducible ecRESCUE RNA base editor: TMP-induced ecDHFR DD expression allows reversible control of the expression of dRanCas13b-ADAR2-mCherry
    Depositor
    Xu Ma
    Article
    Wang et al Cell Commun Signal. 2021 Jul 31;19(1):81
    Insert
    dRanCas13b-hADAR2-mCherry-ecDHFR (folA, ADARB1 Human, R. anatipestifer, E. coli)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    191486-plasmid-map-sequence-id-374351

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  25. ATX3-13Q

    Plasmid
    #184248
    Purpose
    Recombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track fused with His-Tag and TEV cleavage sequence (N-terminal)
    Depositor
    Sandra Macedo Ribeiro
    Article
    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111
    Insert
    ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track (ATXN3 Human, Synthetic)
    Use
    Tags
    6x His-Tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    C-terminal codon optimization for protein expres…
    Promoter
    T7 promoter
    Available since
    Aug. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    184248-plasmid-map-sequence-id-370322

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  28. Venus-Bad-4E-pEGFP-C1

    Plasmid
    #166740
    Purpose
    Express Venus fused to the N-terminus of the Bcl-2 family protein, Bad with mutation in BH3 domain to disrupt binding anti-apoptotic proteins
    Depositor
    David Andrews
    Article
    Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1
    Insert
    Bad-4E (BAD Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    4 hydrophobic residues in the BH3 region mutated …
    Promoter
    CMV
    Available since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    166740-plasmid-map-sequence-id-329000

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Showing: 2371 - 2400 of 4225 results