We narrowed to 8,811 results for: Ott

1. pBLO2 casX

   Plasmid

   #123122

   Purpose

   E. coli expression vector for CasX sgRNA

   Depositor

   Benjamin Oakes, Jennifer Doudna

   Article

   Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

   Insert

   CasX guide RNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Lentiviral CaMKKbeta

   Plasmid

   #33322

   Depositor

   Anthony Means

   Article

   Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

   Insert

   Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mutation

   Promoter

   human PGK

   Available since

   April 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. actc1b-mKate2-rab14

   Plasmid

   #109600

   Purpose

   Muscle specific zebrafish expression of mKate2 fused to rab14

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   rab14 (rab14 Zebrafish)
   

   Use

   Zebrafish plasmids

   Tags

   mKate2

   Expression

   Mutation

   Promoter

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. SauCas9 EMX1-nicking sgRNA

   Plasmid

   #169860

   Purpose

   SauCas9 nicking sgRNA for EMX1

   Depositor

   Scot Wolfe

   Article

   Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

   Insert

   SauCas9 EMX1-nicking sgRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6 promoter

   Available since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1/Hygro(-)-B2-cT

   Plasmid

   #104355

   Purpose

   Expresses human beta 2 integrin tension sensor

   Depositor

   Pontus Nordenfelt, Timothy Springer

   Article

   Nordenfelt et al Nat Commun. 2016 Oct 10;7:13119. doi: 10

   Insert

   Human integrin b2 with tension sensor (ITGB2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCS2-mCherry_SNAP_SNAPV_PACT_Dead

   Plasmid

   #173073

   Purpose

   Dead SNAP tag at the centrosome

   Depositor

   Heidi Hehnly

   Article

   Bucko et al Elife. 2019 Dec 24;8. pii: 52220. doi: 1

   Insert

   mCherry-SNAP-SNAP-Varied-PACT
   

   Use

   Tags

   mCherry-SNAP-SNAP-Varied

   Expression

   Mammalian

   Mutation

   AA144 in both SNAP tags changed from a cysteine t…

   Promoter

   CMV IE94

   Available since

   Aug. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCFJ1208

   Plasmid

   #44490

   Depositor

   Erik Jorgensen

   Article

   Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

   Insert

   Minimal Mos1 [Peft-3 tdTomato(NLS) tbb-2 UTR, unc-119]
   

   Use

   Gateway expression clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEBG p130cas

   Plasmid

   #15001

   Depositor

   Raymond Birge

   Article

   Zvara et al Oncogene. 2001 Feb 22. 20(8):951-61.

   Insert

   p130cas (Bcar1 Rat)
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 22, 2007

   Availability

   Academic Institutions and Nonprofits only

9. JL202: pMVP/mCherry-DEST

   Plasmid

   #121853

   Purpose

   pMVP destination vector, empty expression vector backbone w/ mCherry selection cassette

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Pmvp gateway destination vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. JL002: pMVP/eGFP-DEST

    Plasmid

    #121852

    Purpose

    pMVP destination vector, empty expression vector backbone w/ eGFP selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Pmvp gateway destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. actc1b-lamp1-mKate2

    Plasmid

    #109507

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to lamp1

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    lamp1 (lamp1b Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. JC001: pMVP/Ad/RGD-DEST

    Plasmid

    #121844

    Purpose

    pMVP destination vector, empty RGD fiber-modified adenovirus vector backbone

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Adenoviral; Pmvp gateway destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCRII-Topo Plexin D1 in situ probe

    Plasmid

    #45621

    Depositor

    Jeffrey Macklis

    Article

    Molyneaux et al Neuron. 2005 Sep 15;47(6):817-31.

    Insert

    Plexin D1 in situ probe (Plxnd1 Mouse)
    

    Use

    In situ

    Tags

    Expression

    Mutation

    fragment contains bp# 5751-6406 of NM_026376.3

    Promoter

    Available since

    July 9, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pACYC-Cas12g1-noncoding

    Plasmid

    #120880

    Purpose

    Expresses concatenated noncoding sequences surrounding the CRISPR-Cas12g1 system in the native genomic loci; expresses the tracrRNA required for activity.

    Depositor

    Arbor Biotechnologies

    Article

    Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

    Insert

    Noncoding sequences for CRISPR-Cas12g1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. KN206: pMVP/eCFP-DEST

    Plasmid

    #121854

    Purpose

    pMVP destination vector, empty expression vector backbone w/ eCFP selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Pmvp gateway destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBbdCas9S(-10TC)_Psyn-sgRNA500

    Plasmid

    #149583

    Purpose

    parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30, Psyn

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRKB134_GI-DUX4-intron2(-)

    Plasmid

    #83968

    Purpose

    to test DUX4 mRNA is subjected to NMD upon intron2 inclusion

    Depositor

    Robert Bradley

    Article

    Feng et al Elife. 2015 Jan 7;4. doi: 10.7554/eLife.

    Insert

    DUX4-3'UTR-intron2(-) (DUX4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouseCMV

    Available since

    Jan. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

18. pRKB133_GI-DUX4-intron2(+)

    Plasmid

    #83967

    Purpose

    to test DUX4 mRNA is subjected to NMD upon intron2 inclusion

    Depositor

    Robert Bradley

    Article

    Feng et al Elife. 2015 Jan 7;4. doi: 10.7554/eLife.

    Insert

    DUX4-3'UTR-intron2(+) (DUX4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouseCMV

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pBS-KS-attB2-SA(1)-T2A-QF2-Hsp70

    Plasmid

    #62945

    Purpose

    Creating QF2 drivers from MiMIC lines with inserts into coding introns in Phase 1

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-QF2-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. tdEos-VSVG-16

    Plasmid

    #57693

    Purpose

    Localization: Golgi/ER Transport, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

    Insert

    VSVG
    

    Use

    Tags

    tdEos

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. CS2 Flag-Smad2 (100-467)

    Plasmid

    #14928

    Depositor

    Joan Massague

    Article

    Hata et al Nature. 1997 Jul 3. 388(6637):82-7.

    Insert

    Smad2 (100-467) (SMAD2 Human)
    

    Use

    Xenopus/mammalian/avian/zebrafish

    Tags

    Flag

    Expression

    Mutation

    amino acids 100-467

    Promoter

    Available since

    May 17, 2007

    Availability

    Academic Institutions and Nonprofits only

22. pCph8

    Plasmid

    #22869

    Depositor

    Christopher Voigt

    Article

    Levskaya et al Nature. 2005 Nov 24. 438(7067):441-2.

    Insert

    pCph8
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    First 517 amino acids of Cph1 and the last 229 am…

    Promoter

    Available since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. CS2 Flag-Smad2 (1-185)

    Plasmid

    #14929

    Depositor

    Joan Massague

    Article

    Hata et al Nature. 1997 Jul 3. 388(6637):82-7.

    Insert

    Smad2 (1-185) (SMAD2 Human)
    

    Use

    Xenopus/mammalian/avian/zebrafish

    Tags

    Flag

    Expression

    Mutation

    amino acids 1-185

    Promoter

    Available since

    May 17, 2007

    Availability

    Academic Institutions and Nonprofits only

24. pRSF-G1mCNPRS

    Plasmid

    #174718

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of L-3-(2-cyano-4-pyridyl)alanine (mCNP), into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Abdelkader et al Angew Chem Int Ed Engl. 2022 Jan 31. doi

    Insert

    tRNA synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJet-CMV-CD5Flag2-bglpA

    Plasmid

    #89705

    Purpose

    Expression plasmid for Flag embedded into human CD52 with short polyA

    Depositor

    Dmitriy Mazurov

    Article

    Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

    Insert

    CMV-CD5Flag2-bglpA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBS-KS-attB2-SA(0)-T2A-QF2-Hsp70

    Plasmid

    #62944

    Purpose

    Creating QF2 drivers from MiMIC lines with inserts into coding introns in Phase 0

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-QF2-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBS-KS-attB2-SA(2)-T2A-QF2-Hsp70

    Plasmid

    #62946

    Purpose

    Creating QF2 drivers from MiMIC lines with inserts into coding introns in Phase 2

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-QF2-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. JH401: pMVP/Ad/RGD-PK-DEST

    Plasmid

    #121846

    Purpose

    pMVP destination vector, empty RGD/PK fiber-modified adenovirus vector backbone

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Adenoviral; Pmvp gateway destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMXs_FLAG-SFXN3

    Plasmid

    #110637

    Purpose

    Retroviral expression of Flag-SFXN3 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Science. 2018 Nov 16;362(6416). pii: 362

    Insert

    SFXN3 (SFXN3 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCMV5 Flag-Smad2 (1-456)

    Plasmid

    #14958

    Depositor

    Joan Massague

    Article

    Hata et al Nature. 1997 Jul 3. 388(6637):82-7.

    Insert

    Smad2 (SMAD2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Amino acids 1-456 (tail deletion)

    Promoter

    CMV

    Available since

    May 17, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pBS-KS-attB2-SA(1)-T2A-LexA::GADfluw-Hsp70

    Plasmid

    #78306

    Purpose

    Creating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 1

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-nlsLexA::GADfluw-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pACYC-Cas12c2-noncoding

    Plasmid

    #120877

    Purpose

    Expresses concatenated noncoding sequences surrounding the CRISPR-Cas12c2 system in the native genomic loci; expresses the tracrRNA required for activity.

    Depositor

    Arbor Biotechnologies

    Article

    Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

    Insert

    Noncoding sequences for CRISPR-Cas12c2
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMXs_SFXN5

    Plasmid

    #110640

    Purpose

    Retroviral expression of SFXN5 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Science. 2018 Nov 16;362(6416). pii: 362

    Insert

    SFXN5 (SFXN5 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pGP8GF-AtTIR1

    Plasmid

    #188427

    Purpose

    Arabidopsis Nuclear Auxin Signaling Receptor TIR1

    Depositor

    Britney Moss

    Article

    Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

    Insert

    Auxin Signaling Receptor TIR1 (TIR1 Mustard Weed)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMGS59(AID-ARF-P2A-Hygromycin)

    Plasmid

    #138174

    Purpose

    An AID-ARF-P2A-Hygromycin fusion cassette to tag the protein of interests with auxin-inducible degron and ARF at the c-terminal

    Depositor

    Michael Guertin

    Article

    Sathyan et al Curr Protoc Mol Biol. 2020 Sep;132(1):e1

    Insert

    AID-ARF-P2A-Hygromycin
    

    Use

    Tags

    AID-ARF clamp degron tag

    Expression

    Mutation

    Promoter

    Available since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Lentiviral FLAG-CaMKKbeta

    Plasmid

    #32458

    Depositor

    Anthony Means

    Article

    Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

    Insert

    Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    Feb. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

37. actc1b-mKate2-rab5aa

    Plasmid

    #109648

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to rab5aa

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    rab5aa (rab5aa Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. actc1b-mKate2-rab5ab

    Plasmid

    #109649

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to rab5ab

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    rab5ab (rab5ab Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAAVsc CB6PI Gpluc7XmiR-122T

    Plasmid

    #35647

    Depositor

    Phillip Zamore

    Article

    Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

    Insert

    7 bulged miR-122 target sites
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CB

    Available since

    July 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. HB901: pMVP (L3-L2) polyA

    Plasmid

    #121746

    Purpose

    pMVP L3-L2 entry plasmid, contains human growth hormone polyadenylation signal (polyA) for 3- or 4-component MultiSite Gateway Pro assembly

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    Polyadenylation signal (polyA)
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pMTS-hFluc-HA-GFP11

    Plasmid

    #177726

    Purpose

    Firefly luciferase reporter fused to a mitochondria signal sequence for mitochondrial targeting with an HA tag and the eleventh β-strand of GFP added to the C-terminus

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    hFluc
    

    Use

    Tags

    GFP11, HA, and Mitochondrial Targeting Sequence

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. EGFP-GEM

    Plasmid

    #111191

    Purpose

    fluorescent fusion protein

    Depositor

    Johannes A. Schmid

    Article

    Piddini et al EMBO J. 2001 Aug 1. 20(15):4076-87.

    Insert

    GEM (GEM Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    T2A

    Promoter

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pENTR1A-3xFlag-M694V-MEFV

    Plasmid

    #167118

    Purpose

    Gateway entry plasmid with 3xFlag-pyrin (M694V/FMF variant)

    Depositor

    Thomas Henry

    Article

    Chirita et al Methods Mol Biol. 2022;2523:179-195. doi

    Insert

    MEFV (MEFV Human)
    

    Use

    Gateway entry vector

    Tags

    3xFlag

    Expression

    Mutation

    M694V Methionine 694 to valine (FMF mutation)

    Promoter

    Available since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pBbdCas9S(-10AC12)_Psyn-sgRNArodA

    Plasmid

    #149661

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi rodA

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNArodA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30(-10AC12), Psyn

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. IF701: pMVP (L5-L4) APEX2

    Plasmid

    #121725

    Purpose

    pMVP L5-L4 entry plasmid, contains APEX2 for 4-component MultiSite Gateway Pro assembly. Allows fusion of N-term APEX2 to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    APEX2 (open)
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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46. LC501: pMVP (L3-L2) mCherry + polyA

    Plasmid

    #121765

    Purpose

    pMVP L3-L2 entry plasmid, contains mCherry + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term mCherry fusion to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    mCherry + polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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47. JN607: pMVP/SB-DEST

    Plasmid

    #121860

    Purpose

    pMVP destination vector, empty Sleeping Beauty transposon vector backbone

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Sleeping beauty transposon, pmvp gateway destinat…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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48. Ef1a-QCP-3xBFP

    Plasmid

    #158206

    Purpose

    Qb coat protein fused to three repeats of the blue fluorescent protein under a Efla promoter.

    Depositor

    Roee Amit

    Article

    Katz et al Nat Commun. 2021 Mar 11;12(1):1576. doi:

    Insert

    QCP under the Elfa promoter and three BFP tags
    

    Use

    Synthetic Biology

    Tags

    3xBFP

    Expression

    Mammalian

    Mutation

    Promoter

    Elfa

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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49. pMXs_FLAG-SFXN2

    Plasmid

    #110636

    Purpose

    Retroviral expression of Flag-SFXN2 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Science. 2018 Nov 16;362(6416). pii: 362

    Insert

    SFXN2 (SFXN2 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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50. pBbdCas9S(RBSmut)_Psyn-sgRNAflaB

    Plasmid

    #149588

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi flaB

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNAflaB
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30, Psyn

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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