179,463 results
-
Viral prep#130995-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-hSyn-ChRmine-mScarlet-Kv2.1-WPRE (#130995). In addition to the viral particles, you will also receive purified pAAV-hSyn-ChRmine-mScarlet-Kv2.1-WPRE plasmid DNA. hSyn-driven, soma-targeted ChRmine-mScarlet expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmScarletAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only
-
pAAV mGAD65(delE1) GFP WPRE SV40pA
Plasmid#177316PurposeTo produce the AAV vector that expresses GFP under the control of the mGAD65 promoter. The mGAD65 promoter has highly specificity to GABAergic interneurons.DepositorInsertmGAD65(delE1) promoter, GABAergic interneurons specific promoter
UseAAVAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO mCherry (AAV Retrograde)
Viral prep#114471-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-Ef1a-fDIO mCherry (#114471). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO mCherry plasmid DNA. Ef1a-driven, Flp recombinase-dependent expression of mCherry. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsmCherry (Flp-dependent)Available sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
MoClo CRISPR/Cas Toolkit for Plants
Plasmid kit#1000000159PurposePlasmids for gene editing using Cas nucleases and base editors in monocots and dicots.DepositorApplicationCloning and Synthetic Biology, Genome EditingVector typePlant ExpressionEditing typeCRISPRCloning typeGolden Gate (MoClo)Available sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTP396
Plasmid#149336PurposeBacterial expression of His14-Biotin_acceptor_peptide-SUMOEu1-anti-GFP_nanobodyDepositorInsertanti-GFP nanobody
TagsBiotin acceptor peptide (Avi-tag), His14, and SUM…ExpressionBacterialAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDONR223 SARS-CoV-2 NSP16
Plasmid#141269PurposeGateway-compatible Entry vector, with insert of NSP16 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1DepositorInsertNSP16 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway CloningMutationMany synonymous changes due to codon optimizationAvailable sinceApril 1, 2020AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
Cbh_v5 AAV-CBE N-terminal
Plasmid#137175PurposeAAV genome: expresses the N-terminal of v5 AAV-CBE from the Cbh promoter, and U6-sgRNADepositorInsertv5 AAV-CBE N-terminal; U6-protospacer
UseAAVMutationCas9 D10APromoterCbhAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-FLEX-TeLC-P2A-EYFP-WPRE
Plasmid#135391PurposeCre-dependent Tetanus toxin light chain construct for conditional silencing of neurons.DepositorInsertTeLC-P2A-EYFP
UseAAVExpressionMammalianPromoterhSynapsinAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSMAL-CellTag-V1 (Concentrated Lentiviral Prep)
Viral prep#115643-LVCPurposeReady-to-use Concentrated Lentiviral Prep particles produced from pSMAL-CellTag-V1 (#115643). In addition to the viral particles, you will also receive purified pSMAL-CellTag-V1 plasmid DNA. <p><p>Ready-to-use lentiviral particles carrying version 1 of the CellTag barcoding library to combinatorially index cells for single-cell analysis of clonal dynamics.</p></p>DepositorPromoterMinimal CMVTagsEGFPAvailable sinceOct. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSGAb-km
Plasmid#121999PurposeA sgRNA expression plasmid for genome editing in Acinetobacter baumanniiDepositorInsertnone
UseCRISPRAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
HP17_KO7
Plasmid#120346PurposeHelper plasmid for production of phagemid ssDNA, based on M13KO7DepositorInsertCoding part of the M13KO7 helper phage genome
ExpressionBacterialPromoternative phage promotersAvailable sinceAug. 30, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMK1334
Plasmid#127965PurposesgRNA expression vector compatible with CROP-Seq and imaging screensDepositorInsertEF1a-Puro-T2A-2xmycNLS-WPRE-mU6-sgRNA
UseCRISPR and LentiviralExpressionMammalianPromotermU6Available sinceAug. 24, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CMV-mCherry-(F30-2xPepper)10
Plasmid#129401PurposemCherry mRNA reporter tagged with (F30-2xPepper)10DepositorInsertmCherry-(F30-2xPepper)10
ExpressionMammalianPromoterCMVAvailable sinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET-32a(+)-NRG1B1
Plasmid#120287PurposeProduce human recombinant NRG1 in DE3 cellsDepositorInsertNRG1-beta 1
Tags6x His and S-TagExpressionBacterialMutationCodon optimized for E. Coli productionPromoterT7 promoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA-Halotag-FRB-EX-nes_pLX304
Plasmid#120913PurposeLentiviral vector expressing HA-Halotag-FRB-EX-NES in mammalian cytosolDepositorInsertHA-Halotag-FRB-EX-NES
UseLentiviralExpressionMammalianAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJMP1039
Plasmid#119239Purposeexpresses Tn7 transposaseDepositorInserttnsABCD
ExpressionBacterialAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pmGFP-ADAR2
Plasmid#117929PurposeADAR2 expression plasmid.DepositorAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CAG-NLS-GFP
Plasmid#104061PurposeAAV expression of nuclear localized GFP with SV40 NLSDepositorHas serviceAAV PHP.eBInsertGFP
UseAAVPromoterCAGAvailable sinceAug. 31, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40 (AAV Retrograde)
Viral prep#100853-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40 (#100853). In addition to the viral particles, you will also receive purified pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40 plasmid DNA. Synapsin-driven, Cre-dependent GECO1a calcium sensor. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
Bassik lab Human CRISPR-Cas9 Deletion Library - Drug targets, kinases, phosphatases
Pooled library#101927PurposeBassik lab Human CRISPR-Cas9 Deletion Library - Drug targets, kinases, phosphatases. Coexpresses mCherry.DepositorExpressionMammalianUseCRISPRAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV.CAG.Flex.NES-jRCaMP1a.WPRE.SV40 (AAV1)
Viral prep#100846-AAV1PurposeReady-to-use AAV1 particles produced from pAAV.CAG.Flex.NES-jRCaMP1a.WPRE.SV40 (#100846). In addition to the viral particles, you will also receive purified pAAV.CAG.Flex.NES-jRCaMP1a.WPRE.SV40 plasmid DNA. CAG-driven, Cre-dependent jRCaMP1a calcium sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hChR2(H134R)-mCherry (AAV1)
Viral prep#26975-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-CaMKIIa-hChR2(H134R)-mCherry (#26975). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hChR2(H134R)-mCherry plasmid DNA. CaMKIIa-driven, humanized channelrhodopsin H134R mutant fused to mCherry for optogenetic activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIITagsmCherryAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
IGI-P0492 pHR-dCas9-NLS-VPR-mCherry
Plasmid#102245PurposeLentiviral expression of dCas9-VPR-mCherry fusion protein for CRISPRa.DepositorInsertdCas9-VPR-mCherry
UseCRISPR and LentiviralTagsNLS-VPR-mCherryExpressionMammalianPromoterCAGAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-hLbCpf1(TYCV) (pY230)
Plasmid#89355PurposeExpresses humanized LbCpf1 variant that recognizes TYCV PAMsDepositorInsertCpf1, RR variant
UseCRISPRTagsNLS and NLS-3xHAExpressionMammalianMutationG532R/K595RPromoterCMVAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJYS3_ΔcrtYf
Plasmid#85542PurposeConstitutive transcription of FnCpf1 and crRNA of crtYfDepositorInsertsCpf1
crRNA of crtYf
UseCRISPR; Shuttle vector corynebacterium glutamicum…ExpressionBacterialAvailable sinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-RAM-d2TTA::TRE-MCS-WPRE-pA
Plasmid#63931PurposeEmpty AAV-RAM plasmid w. multiple cloning site (MCS)DepositorTypeEmpty backboneUseAAVPromoterpRAM and TREAvailable sinceDec. 11, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Talin-TS
Plasmid#83376PurposeFRET probe for measuring tension on Talin moleculeDepositorInsertTalin1 (Tln1 Mouse)
UseRetroviralTagsEGFP-F40-tagRFP (tension sensor module)ExpressionMammalianMutationTension sensor module is inserted between V447 an…PromoterCMVAvailable sinceOct. 26, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1_pCMV-nCas-PmCDA1-ugi pH1-gRNA(HPRT)
Plasmid#79620PurposeExpresses nCas9-PmCDA1-UGI and gRNA(HPRT) in mammalian cellsDepositorPublicationInsertSpCas9
TagsPmCDA1 and UGIExpressionMammalianMutationD10A for nickase Cas9PromoterpCMVAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMSCV-Zeo
Plasmid#75088Purposeretrovirus-mediated gene transfer into mammalian cellsDepositorTypeEmpty backboneUseRetroviralExpressionMammalianAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYPQ152
Plasmid#69303PurposeGateway entry vector with pco-dCas9-VP64DepositorInsertpco-dCas9-VP64 (Plant codon-optimized)
UseCRISPRMutationVP64 activator fusion; NLS fusionAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDD284
Plasmid#66825PurposeTagRFP-T^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertTagRFP-T-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags3xFlag and C. elegans codon-optimized TagRFP-TExpressionWormAvailable sinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAcBac2.tR4-OMeYRS/GFP*
Plasmid#50831Purposebaculovirus-based delivery system that enables the efficient incorporation of unnatural amino acids into proteins in mammalian cellsDepositorInsertstwo-copy Tyr tRNA cassette
EGFP*
OMeYRS
two copy Tyr tRNA cassette
UseBaculoviralTagsHis, Myc-6xHis, and WPREExpressionInsectMutationY39TAG and able to charge various unnatural amino…PromoterCAG, CMV, and U6 and H1Available sinceJune 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSIN4-EF1a-ETV2-IRES-Puro
Plasmid#61061Purposelentiviral vector for ETV2DepositorAvailable sinceMarch 27, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSBbi-Neo
Plasmid#60525PurposeSB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: neomycin resistance geneDepositorTypeEmpty backboneUseTransposonExpressionMammalianPromoterEF1a/RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSBtet-RP
Plasmid#60497PurposeSB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of RFP, rtTA and puromycin resistance geneDepositorTypeEmpty backboneUseTransposonExpressionMammalianPromoterTCE & RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAC-BETA
Plasmid#53272PurposeContains crtE, crtB, crtI, and crtY carotenoid pathway genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces beta-carotene in Escherichia coliDepositorInsertcrtE, crtY, crtI, crtB
UseLow copy number bacterial cloning vectorPromoterendogenous promotersAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
EGFP-Actin-7
Plasmid#56421PurposeLocalization: Actin, Excitation: 488, Emission: 507DepositorAvailable sinceDec. 23, 2014AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
paavCAG-Jx-rev-post-mGRASP-2A-dTomato
Plasmid#34913PurposeJxON-postDepositorInsertpostsynaptic mGRASP
UseAAV and Cre/LoxPromoterCAGAvailable sinceNov. 15, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMito-iRFP670
Plasmid#45462DepositorInsertiRFP670
Tagshuman cytochrome C oxidase subunit VIIIExpressionMammalianMutationT2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…PromoterCMVAvailable sinceJune 17, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP-TRIM28
Plasmid#45568DepositorInsertTRIM28 (TRIM28 Human)
TagsEGFPExpressionMammalianMutationG34S mutation in EGFPPromoterCMVAvailable sinceMay 29, 2013AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
human EB1 fused to monomeric RFP
Plasmid#39323DepositorAvailable sinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBB550
Plasmid#27396DepositorInsertdnaK, dnaJ, GroESL
ExpressionBacterialAvailable sinceSept. 7, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
beta-arrestin1 mYFP
Plasmid#36916DepositorAvailable sinceJune 27, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28a-SpyTagMBP
Plasmid#35050DepositorInsertSpyTagMBP
TagsHis6ExpressionBacterialMutationSimple peptide tag added to the N-terminus of MBP…PromoterT7 promoterAvailable sinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti-PGK-ER-KRAS(G12V)
Plasmid#35635DepositorPublicationInsertK-Ras4B(G12V) (KRAS Human)
UseLentiviralTagsERExpressionMammalianMutationGlycine 12 to ValinePromoterPGKAvailable sinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGL3-mArg1 promoter/enhancer -31/-3810
Plasmid#34571DepositorAvailable sinceJan. 17, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
B23(NPM)
Plasmid#34553DepositorAvailable sinceDec. 14, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA-SADB19P
Plasmid#32631DepositorPublicationInsertSADB19P
ExpressionMammalianPromoterCMVAvailable sinceOct. 26, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
HSF1-GFPN3
Plasmid#32538DepositorInsertHSF1 (HSF1 Human)
TagsEGFPExpressionMammalianMutationsee depositor comments belowPromoterCMVAvailable sinceOct. 13, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Snail_pGL2
Plasmid#31694DepositorPublicationAvailable sinceSept. 21, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by