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We narrowed to 5,579 results for: PEP

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Showing: 4351 - 4400 of 5579 results

  2. pOCC17

    Plasmid
    #118875
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal tagRFP
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C--NotI-ccdB-AscI-tagRFP-stop-HindIII cassette
    Tags
    HIS6, cleavable with 3C protease and tagRFP
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118875-plasmid-map-sequence-id-232352

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  3. pOCC16

    Plasmid
    #118870
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal EGFP
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C--NotI-ccdB-AscI-EGFP-stop-HindIII cassette
    Tags
    EGFP and HIS6, cleavable with 3C protease
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118870-plasmid-map-sequence-id-232333

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  4. pOCC218

    Plasmid
    #118907
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS10, cleavable with 3C protease and N-terminal YBBR tag
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS10-3C-YBBR-NotI-ccdB-AscI-stop-HindIII cassette
    Tags
    HIS10, cleavable with 3C protease, YBBR tag
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118907-plasmid-map-sequence-id-232329

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  5. pOCC18

    Plasmid
    #118868
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal tagRFP
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C-tagRFP-NotI-ccdB-AscI-stop-HindIII cassette
    Tags
    HIS6, cleavable with 3C protease, tagRFP
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118868-plasmid-map-sequence-id-232324

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  6. pOCC171

    Plasmid
    #118888
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal SNAP tag
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-MBP-3C-SNAP-NotI-ccdB-AscI-stop-HindIII cassette
    Tags
    HIS6-MBP, cleavable with 3C protease; SNAP tag
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118888-plasmid-map-sequence-id-232245

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  7. pOCC202

    Plasmid
    #118880
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal mKate2, and C-terminal HIS6 tag, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-NotI-ccdB-AscI-mKate2-3C-HIS6-stop-HindIII cassette
    Tags
    mKate2, HIS6, cleavable with 3C protease
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118880-plasmid-map-sequence-id-232243

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  8. pOCC139

    Plasmid
    #118872
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal monomeric GFP
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C--NotI-ccdB-AscI-mGFP-stop-HindIII cassette
    Tags
    HIS6, cleavable with 3C protease and mGFP
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118872-plasmid-map-sequence-id-232236

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  9. pYPQ127B

    Plasmid
    #99883
    Purpose
    Express two TALE-VP64 activator linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequence under 2x35S promoter
    Depositor
    Yiping Qi
    Article
    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(
    Insert
    TALE-VP64-T2A-TALE-VP64
    Use
    TALEN
    Expression
    Plant
    Available Since
    March 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    99883-plasmid-map-sequence-id-203133

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  14. pET-Ce25

    Plasmid
    #51296
    Purpose
    Expresses a plant arginyl-tRNA synthetase in E.coli
    Depositor
    Gabor Igloi
    Article
    Hogg et al Protein Expr Purif. 2008 Oct;61(2):163-7
    Insert
    arginyl-tRNA synthetase
    Tags
    His Tag and Thioredoxin
    Expression
    Bacterial
    Mutation
    GTT (Val) insertion after initiation codon to per…
    Promoter
    T7
    Available Since
    July 31, 2014
    Availability
    Academic Institutions and Nonprofits only
    51296-plasmid-map-sequence-id-78029

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  16. pEG593

    Plasmid
    #40119
    Depositor
    Geraldine Seydoux
    Article
    Griffin et al Cell. 2011 Sep 16;146(6):955-68.
    Insert
    Mex-5 (ATG through 648 bp of 3'UTR) R274E K318E (RNAi res) (mex-5 Nematode)
    Use
    Gateway destination vector
    Tags
    Dendra2 PAFP (sequence optimized for worm express…
    Expression
    Worm
    Mutation
    R274E, K318E, Exon 2 recoded to be RNAi-resistant
    Promoter
    4.4 kb mex-5 promoter fragment
    Available Since
    Oct. 4, 2012
    Availability
    Academic Institutions and Nonprofits only

  17. pEG628

    Plasmid
    #40120
    Depositor
    Geraldine Seydoux
    Article
    Griffin et al Cell. 2011 Sep 16;146(6):955-68.
    Insert
    Mex-5 (aa1-244) (RNAi resistant) (mex-5 Nematode)
    Use
    Gateway destination vector
    Tags
    Dendra2 PAFP (sequence optimized for worm express…
    Expression
    Worm
    Mutation
    contains aa1-244, Exon 2 recoded to be RNAi-resis…
    Promoter
    4.4 kb mex-5 promoter fragment
    Available Since
    Sept. 6, 2012
    Availability
    Academic Institutions and Nonprofits only

  25. AIO-Puro

    Plasmid
    #74630
    Purpose
    All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and Cas9-D10A nickase linked via 2A peptide with puromycin resistant marker to enhance efficient and accurate genome editing
    Depositor
    Steve Jackson
    Article
    Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Puromycin resistant marker
    Expression
    Mammalian
    Promoter
    Cbh
    Available Since
    May 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    74630-plasmid-map-sequence-id-373032

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  28. pJRH-1345 U6-B2M sgRNA Gag-Cas9 v2

    Plasmid
    #201916
    Purpose
    Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103
    Inserts
    Gag-Cas9 v2
    B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
    Expression
    Mammalian
    Promoter
    CAG and Human U6
    Available Since
    Aug. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    201916-plasmid-map-sequence-id-403306

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  30. MTNR1B-DuET

    Plasmid
    #213349
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    MTNR1B (MTNR1B Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213349-plasmid-map-sequence-id-430875

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  31. OPRM1-DuET

    Plasmid
    #213361
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    OPRM1 (OPRM1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    213361-plasmid-map-sequence-id-431826

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  33. MTNR1A-DuET

    Plasmid
    #213348
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    MTNR1A (MTNR1A Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213348-plasmid-map-sequence-id-430874

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  35. pDONR P2R-P3-SV40 polyA signal

    Plasmid
    #48627
    Purpose
    Contributes a cassette containing an SV40 polyA signal as the 3’-module during MultiSite Gateway cloning of chimeric cDNAs, when a peptide module at the C-terminal end is not required.
    Depositor
    Anna Planas, Tomas Santalucia
    Article
    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:
    Insert
    SV40 early polyadenylation signal cassette
    Use
    Gateway entry vector
    Promoter
    none
    Available Since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    48627-plasmid-map-sequence-id-70632

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  44. pYPQ173

    Plasmid
    #99907
    Purpose
    Express CRISPR-Act2.0 system which contains pco-dCas9-VP64 fusion protein and MS2-VP64 fusion protein linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequence
    Depositor
    Yiping Qi
    Article
    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(
    Insert
    pco-dCas9-VP64-T2A-MS2-VP64
    Use
    CRISPR
    Expression
    Plant
    Available Since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    99907-plasmid-map-sequence-id-210432

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  46. pJRoC30_Tv9nL

    Plasmid
    #188069
    Purpose
    PROSS-designed high-redox potential laccase from Trametes versicolor
    Depositor
    Sarel Fleishman
    Article
    Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173
    Insert
    PROSS-designed high-redox potential laccase from Trametes versicolor
    Tags
    S. cerevisiae evolved α-factor prepro-leader sign…
    Expression
    Yeast
    Mutation
    79 PROSS mutations, described in publication
    Available Since
    Oct. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    188069-plasmid-map-sequence-id-370129

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Showing: 4351 - 4400 of 5579 results