We narrowed to 11,159 results for: ENA
-
Plasmid#216012PurposeFragmid fragment: (Cas protein) deactivated CasDepositorHas ServiceCloning Grade DNAInsertdCas9_v1.1 [Sp]
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYY1704
Plasmid#198421PurposepBs3:RedNTF:tNOS. A pGreen based plasmid expressing the INTACT reporter RedNTF after the promoter of the pepper Bs3 gene (pBs3). The plasmid has spectinomycin resistance.DepositorInsertRedNTF
UseTagsExpressionPlantMutationPromoterpBs3Available sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTol2-elavl3-WHaloCaMP1a
Plasmid#205312PurposeExpression of WHaloCaMP1a in zebrafish from the elavl3 promoter (pan-neuronal expression)DepositorInsertWHaloCaMP1a
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-LINXa3-nano
Plasmid#73616PurposeLight-induced nuclear export switch fusion to nano for microscopy in mammalian cells.DepositorInsertLINXa3-nano
UseTagsmCherryExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-DENV2A-GFP-puro
Plasmid#140089PurposeFluorescence activatable reporter of DENV-2 NS2B-NS3 protease activityDepositorInsertDENV2A-GFP
UseLentiviral; Low expressionTags6XHis tag and GFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
GST-Bcr pLEF
Plasmid#38160DepositorInsertBcr (BCR Human)
UseTagsGSTExpressionMammalianMutationPromoterSV40Available sinceAug. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Tsc2-g1)-PGKpuroBFP-W
Plasmid#105039PurposeLentiviral gRNA plasmid targeting mouse Tsc2 , co-expression of TagBFPDepositorInsertTsc2 (Tsc2 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRvSAHS1-mEGFP-NLS
Plasmid#205005PurposeThe vector contains 1kbp of the upstream and downstream regions of the sahs1 gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.DepositorInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the sahs1 gene from Ramazzottius varieornatus
UseTardigrade expressionTagsExpressionMutationPromoterAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRvCAHS3-mEGFP-NLS
Plasmid#205004PurposeThe vector contains 1kbp of the upstream and downstream regions of the cahs3 gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.DepositorInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the cahs3 gene from Ramazzottius varieornatus
UseTardigrade expressionTagsExpressionMutationPromoterAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJH4504
Plasmid#191702PurposePnmr-1 long::TeTx::wCherry unc-54 3' UTR C.elegans AVA/other neurons expression of TeTx RFPDepositorInsertTetanus Toxin Light Chain
UseTagswCherryExpressionWormMutationPromoterPnmr-1 longAvailable sinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDFNKI-strepII-3C-LIC-strep
Plasmid#108708PurposeExpression of a StrepII-tagged target protein with 3C protease cleavage site.DepositorTypeEmpty backboneUseTagsStrepII-3C protease cleavage siteExpressionBacterialMutationPromoterT7Available sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
mVenus-NES-SspB-LARG-DH-P2A-iLIDcaax
Plasmid#173863PurposeMammalian expression of mVenus-NES-SspB-LARG-DH-P2A-iLIDcaax (opto-RhoA)DepositorInsertmVenus-NES-SspB-LARG-DH-P2A-iLIDcaax
UseTagsC-terminal CAAX-box and mVenusExpressionMammalianMutationPromoterCMVAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJG367
Plasmid#91185PurposeBeYDV replicon T-DNA for gene targeting in tobacco leaves, H840A double nickase (nAtCas9_H840A+gNt_R2+gNt_F2+donor)DepositorInsertnAtCas9_H840A+gNt_R2+gNt_F2+donor
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJG382
Plasmid#91189PurposeBeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A double nickase (nAtCas9_D10A+gNt_R2+gNt_F2+donor)DepositorInsertnAtCas9_D10A+gNt_R2+gNt_F2+donor
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf106
Plasmid#12794PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf106
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
mVenus-NES-SspB-C3Gcat-P2A-iLIDcaax
Plasmid#173868PurposeMammalian expression of mVenus-NES-SspB-C3Gcat-P2A-iLIDcaax (opto-Rap)DepositorInsertmVenus-NES-SspB-C3Gcat-P2A-iLIDcaax
UseTagsC-terminal CAAX-box and mVenusExpressionMammalianMutationPromoterCMVAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
mVenus-NES-SspB-SOS2cat-P2A-iLIDcaax
Plasmid#173867PurposeMammalian expression of mVenus-NES-SspB-SOS2cat-P2A-iLIDcaax (opto-Ras)DepositorInsertmVenus-NES-SspB-SOS2cat-P2A-iLIDcaax
UseTagsC-terminal CAAX-box and mVenusExpressionMammalianMutationPromoterCMVAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
AA406
Plasmid#216070PurposeFragmid fragment: (2A Selection) Violet-excited GFP, T2A, blasticidin resistanceDepositorHas ServiceCloning Grade DNAInsertP2A_v1.1; VexGFP_v1.1; T2A_v1.1; BlastR_v1.1; Stop
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
NSP6-FR-2Strep
Plasmid#210317PurposeExpresses SARS-CoV-2 NSP6 in mammalian cellsDepositorInsertSARS-CoV-2 NSP6 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
UseTags2StrepExpressionMammalianMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMOD_B2309
Plasmid#91069PurposeModule B, Promoter: YAO, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35SDepositorInsertSapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers
UseCRISPRTagsExpressionMutationPromoterYAOAvailable sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCSN061
Plasmid#101725PurposeSingle copy yeast plasmid expressing Cas9 from Streptococcus pyogenes (SpCas9), codon optimized for expression in Saccharomyces cerevisiae.DepositorInsertsS. pyogenes Cas9 codon optimized for expression in S. cerevisiae. (NEWENTRY )
KanMX marker expression cassette.
UseCRISPRTagsSV40 NLSExpressionYeastMutationPromoterHeterologous TEF1 promoter from A. gossypii. and …Available sinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-RIPK3 4KR
Plasmid#78813PurposeTo overexpress RIPK3 4KR in Mammalian CellDepositorInsertRIPK3 (RIPK3 Human)
UseTagsHAExpressionMammalianMutationK55R, K89R, K363R, K501RPromoterCMVAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEXNKI-his-GST-3C-LIC
Plasmid#108709PurposeExpression of a GST-6xHis-tagged target protein with 3C protease cleavage site.DepositorTypeEmpty backboneUseTagsGST-6xHis-3C protease cleavage siteExpressionBacterialMutationPromotertacAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf37
Plasmid#12725PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf37
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf48
Plasmid#12736PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf48
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAAV-β2AR-NNES-NanoLuc-eLOV-TEVcs-Flag-Gal4-V5
Plasmid#125236PurposeExpresses modified SPARK β2AR-NanoLuc transmembrane component in mammalian cellsDepositorInsertβ2AR-NNES-NanoLuc-eLOV-TEVcs-Flag-Gal4-V5
UseAAVTagsFlag, V5ExpressionMammalianMutationPromoterCMVAvailable sinceJune 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-VCPIP (OTU, aa 25-561)
Plasmid#61583PurposeExpresses human VCPIP (OTU domain) in E. coli.DepositorInsertVCPIP (VCPIP1 Human)
UseTagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-24 and aa 562-1222.PromoterT7Available sinceDec. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
8xCTS1_WT-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_WT-hutRNApro_SV40polyA
Plasmid#124546PurposeCloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.DepositorInsertWT-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_WT-hutRNApro
UseTagsExpressionMammalianMutationWT, WTPromoterMinimal ADEAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf40
Plasmid#12728PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf40
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
ND4 left TALE–G1333 DddAtox C
Plasmid#158092Purposeexpresses the right-side ND4 TALE–DddAtox half in mammalian cellsDepositorInsertSOD2 MTS–3xHA–ND4 left TALE–G1333 DddAtox-C–1xUGI
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40
Plasmid#133560PurposeBackbone vector for CRISPR-HDR insertion of SPARC-Variant constructs (or any module) near the attP40 locusDepositorTypeEmpty backboneUseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-TNKS-2
Plasmid#154149PurposeFor making TNKS-2 RNADepositorInsertTankyrase 2 (TNKS2 Human)
UseIn vitro transcriptionTagsFLAGExpressionMutationPromoterT7Available sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDH-Lenti-CAGGS-hPRDM6-2xGly-V5-EF1-Luc2-Blast
Plasmid#213826PurposeExpresses human PRDM6 with a C-terminal V5 epitope tag.DepositorInsertPRDM6 (PRDM6 Human)
UseLentiviral and LuciferaseTagsV5ExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Tsc2-g2)-PGKpuroBFP-W
Plasmid#105040PurposeLentiviral gRNA plasmid targeting mouse Tsc2 , co-expression of TagBFPDepositorInsertTsc2 (Tsc2 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
MSCV mouse Vav1 IRES GFP
Plasmid#107091PurposeMammalian expression retroviral vectorDepositorInsertVav1 (Vav1 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETNKI-his-3C-LIC-kan
Plasmid#108703PurposeExpression of an His-tagged target protein with 3C protease cleavage site.DepositorTypeEmpty backboneUseTagsHis-3C protease cleavage siteExpressionBacterialMutationPromoterT7Available sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC008_pLKO-U6-BsmBI-chRNA(+85)-EFS-Thy11
Plasmid#192147PurposeT cell CRISPR lentiviral vectorDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationNAPromoterAvailable sinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-ASAP2f
Plasmid#183757PurposeBxb1-specific donor plasmid for intergrating a transcription unit for expression of ASAP2f voltage reporterDepositorInsertASAP2f
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMA7
Plasmid#79967PurposeTM-MAGE strainDepositorInsertsLambda Red recombinase beta subunit
DNA adenine methylase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpBADAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDFNKI-his-3C-LIC-strep
Plasmid#108705PurposeExpression of a His-tagged target protein with 3C protease cleavage site.DepositorTypeEmpty backboneUseTagsHis-3C protease cleavage siteExpressionBacterialMutationPromoterT7Available sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
MAC_N_ARP3B
Plasmid#111680PurposeMAC-tagged gene expressionDepositorInsertARP3B (ACTR3B Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
mVenus-NES-SspB-RGL2-GEF-P2A-iLIDcaax
Plasmid#173875PurposeMammalian expression of mVenus-NES-SspB-RGL2-GEF-P2A-iLIDcaax (opto-Ral)DepositorInsertmVenus-NES-SspB-RGL2GEF-P2A-iLIDcaax
UseTagsC-terminal CAAX-box and mVenusExpressionMammalianMutationPromoterCMVAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry-NES-SspB-RGL2-GEF-P2A-iLIDcaax
Plasmid#173876PurposeMammalian expression of mCherry-NES-SspB-RGL2-GEF-P2A-iLIDcaax (opto-Ral)DepositorInsertmCherry-NES-SspB-RGL2GEF-P2A-iLIDcaax
UseTagsC-terminal CAAX-box and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_21B
Plasmid#91129PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9_dead + AtU6:gRNA, Plant Selection: 2x35S:hpt IIDepositorInsertEngineering Reagent: 35S:AtCas9_dead + AtU6:gRNA
UseCRISPRTagsExpressionPlantMutationD10A, H840APromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf107
Plasmid#12795PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf107
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
dmBACCS2VL-IRES-dOrai
Plasmid#67629PurposeMammalian expression of dmBACCS2VL, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, and dOraiDepositorInsertdmBACCS2VL-IRES-dOrai
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
ND4 right TALE–G1333 DddAtox N
Plasmid#158096Purposeexpresses the right-side ND4 TALE–DddAtox half in mammalian cellsDepositorInsertCOX8A MTS–3xFLAG–ND4 right TALE–G1333 DddA tox-N–1xUGI
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
sg1+sg2+sg3
Plasmid#113969PurposeTriple short guide RNA targeting GTATAGCATACATTATACG, TACCACATTTGTAGAGGTT & CAATGTATCTTATCATGTCDepositorInsertsg1+sg2+sg3
UseTagsExpressionMammalianMutationPromoterU6Available sinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUD1 (OTU, aa 287-437)
Plasmid#61407PurposeExpresses human OTUD1 (OTU domain) in E. coli.DepositorInsertOTUD1 (OTUD1 Human)
UseTagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-286 and aa 438-481.PromoterT7Available sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
R0010 (pLacI)_AB
Plasmid#66004PurposeMoClo Basic Part: Controllable promoter - pLacI - lacI regulated (repressed by LacI+CAP. LacI, C0012, inhibited by IPTG) [A:R0010:B]DepositorInsertControllable promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only