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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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173,361 results

Showing: 3921 - 3960 of 173361 results

  1. pAAVS1-NDi-CRISPRi (Gen1)

    Plasmid
    #73497
    Purpose
    Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus with mCherry marker
    Depositor
    Bruce Conklin
    Article
    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.
    Inserts
    dCas9-KRAB-P2A-mCherry
    rtTA
    Use
    CRISPR
    Tags
    HA, KRAB, and NLS
    Expression
    Mammalian
    Mutation
    D10A, H840A (catalytically deactivated Cas9)
    Promoter
    CAG and TRE3G
    Available Since
    Dec. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    73497-plasmid-map-sequence-id-247304

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  5. pBA-mCherry-EGFP-PIM

    Plasmid
    #111758
    Purpose
    Low level expression of mCherry-EGFP-PIM. Can be used for inducible aggregate formation upon AP20187 addition
    Depositor
    Lukas Kapitein
    Article
    Janssen et al Nat Commun. 2018 Oct 12;9(1):4245. doi:
    Insert
    2x FKBP homo-mCherry-EGFP-2x FKBP homo-2x FKBP
    Expression
    Mammalian
    Mutation
    Val24Glu, Tyr80Cys, Ala95Thr mutations in second …
    Promoter
    chicken beta-actin
    Available Since
    Oct. 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    111758-plasmid-map-sequence-id-222504

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  8. pAG CMV Empty Puro

    Plasmid
    #237881
    Purpose
    Empty mammalian expression plasmid for cloning untagged proteins
    Depositor
    Meghan Rego, Addgene Research Program
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 12, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237881-plasmid-map-sequence-id-469912

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  10. pLEX307-DPP4-puro

    Plasmid
    #158451
    Purpose
    Constitutive lentiviral expression of human DPP4.
    Depositor
    Alejandro Chavez, Sho Iketani
    Insert
    DPP4 (DPP4 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158451-plasmid-map-sequence-id-308170

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  12. EF1a- gfp-2a-puro

    Plasmid
    #129443
    Purpose
    expression of GFP and Puro R
    Depositor
    Oskar Laur
    Insert
    gfp-puro
    Use
    Lentiviral
    Available Since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    129443-plasmid-map-sequence-id-256478

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  18. pTP1172

    Plasmid
    #104161
    Purpose
    Bacterial expression plasmid of anti-mouse kappa chain nanobody TP1170 (3x Cysteines)
    Depositor
    Dirk Görlich
    Article
    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154
    Insert
    Anti-mouse kappa chain specific nanobody TP1170 (3xCysteines)
    Tags
    14xHistidine tag and NEDD8 from Brachypodium dist…
    Expression
    Bacterial
    Available Since
    Dec. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    104161-plasmid-map-sequence-id-200072

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  19. pCC_05 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139090
    Purpose
    Expresses human codon-optimized inactive SpCas9 fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    dSpCas9-VPR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139090-plasmid-map-sequence-id-273886

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  25. Halo-Sec61-C-18

    Plasmid
    #123285
    Purpose
    Localization: Endoplasmic Reticulum
    Depositor
    Kevin McGowan
    Insert
    Sec61 (SEC61B Human)
    Tags
    HaloTag7
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    123285-plasmid-map-sequence-id-242690

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  26. pAAV-Ef1a-Con/Fon-GCaMP6F (AAV5)

    Viral Prep
    #137122-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-Ef1a-Con/Fon-GCaMP6F (#137122). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Con/Fon-GCaMP6F plasmid DNA. EF1a-driven, Cre and Flp-dependent expression of GCaMP6f. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth, INTRSECT 2.0 Project
    Article
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    EF1a
    Available Since
    July 17, 2020
    Availability
    Academic Institutions and Nonprofits only

  27. pAAV-Ef1a-DIO ChETA-EYFP (AAV9)

    Viral Prep
    #26968-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-Ef1a-DIO ChETA-EYFP (#26968). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO ChETA-EYFP plasmid DNA. EF1a-driven, Cre-dependent, ChETA fused to EYFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Article
    Gunaydin et al Nat Neurosci. 2010 Mar;13(3):387-92. doi
    Promoter
    EF1a
    Tags
    EYFP (Cre-dependent)
    Available Since
    March 30, 2018
    Availability
    Academic Institutions and Nonprofits only

  33. AAVS1_CAG_OsTIR1_F74G_S210A

    Plasmid
    #242189
    Purpose
    AID 2.0's higher degradation efficiency came with target-specific basal degradation and slower recovery rates. To address these limitations, we employed base-editing-mediated mutagenesis followed by s
    Depositor
    Mazhar Adli
    Article
    Xing et al Nat Commun. 2025 Jul 18;16(1):6631. doi:
    Insert
    CAG_OsTIR1_F74G_S210A
    Expression
    Mammalian
    Mutation
    S210A
    Promoter
    CAG
    Available Since
    Dec. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    242189-plasmid-map-sequence-id-495018

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  37. AAV-CamKIIa-jGCaMP8f-WPRE (AAV9)

    Viral Prep
    #176750-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from AAV-CamKIIa-jGCaMP8f-WPRE (#176750). In addition to the viral particles, you will also receive purified AAV-CamKIIa-jGCaMP8f-WPRE plasmid DNA. CamKIIa-driven expression of calcium sensor GCaMP8f. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Loren Looger
    Promoter
    CamKIIalpha
    Available Since
    Nov. 13, 2025
    Availability
    Academic Institutions and Nonprofits only

  39. pLV-dCas9-p300-P2A-PuroR

    Plasmid
    #83889
    Purpose
    Lentiviral Sp dCas9-p300-P2A-PuroR
    Depositor
    Charles Gersbach
    Article
    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038
    Inserts
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
    pac from Streptomyces alboniger
    Use
    CRISPR and Lentiviral
    Tags
    Flag and nucleoplasmin NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EFS
    Available Since
    April 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    83889-plasmid-map-sequence-id-297764

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Showing: 3921 - 3960 of 173361 results