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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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159,481 results

Showing: 3331 - 3360 of 159481 results

  1. EB1-tdTomato

    Plasmid
    #50825
    Purpose
    expresses EB1-tdTomato fusion in mammalian cells for labeling the growing tips of MTs
    Depositor
    Erik Dent
    Insert
    EB1 (Mapre1 Mouse)
    Use
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    50825-plasmid-map-sequence-id-377726

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  2. pBiFC-VC155

    Plasmid
    #22011
    Purpose
    Mammalian expression of a fluorescent protein fragment derived from Venus for BiFC and multicolor BiFC assays
    Depositor
    Chang-Deng Hu
    Article
    Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.
    Insert
    VC155
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Changed Alanine 206 to Lysine
    Promoter
    Available since
    Sept. 28, 2009
    Availability
    Academic Institutions and Nonprofits only
    22011-plasmid-map-sequence-id-354492

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  6. pHR_PGK_SNIPR_Hinge Notch SNIPR (LaG17)

    Plasmid
    #188380
    Purpose
    Lentiviral vector - constitutive expression of an anti-GFP SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a Gal4VP64 transcriptional factor
    Depositor
    Kole Roybal
    Article
    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.
    Insert
    PGK_antiGFP_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_Gal4VP64
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    188380-plasmid-map-sequence-id-426083

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  7. pSL1521 (pSPIN, pSC101* backbone)

    Plasmid
    #160729
    Purpose
    Single-plasmid V. cholerae CAST system. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA has BsaI sites for cloning. Temperature-sensitive pSC101* backbone can be cured by 37 °C incubation.
    Depositor
    Samuel H. Sternberg
    Article
    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103
    Insert
    VchCAST proteins, crRNA, and donor DNA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119
    Available since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    160729-plasmid-map-sequence-id-322926

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  13. pACYC-LIC+

    Plasmid
    #62312
    Purpose
    E. coli pACYC based expression vector with N-terminal Hexa-HIS and a thrombin protease cleavage site
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    6xHis and thrombin protease cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO
    Available since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  22. TFORF1207

    Plasmid
    #144089
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    CASZ1 (CASZ1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    144089-plasmid-map-sequence-id-396173

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  23. pRN120

    Plasmid
    #160696
    Purpose
    BeYDV viral replicon on T-DNA backbone expressing Firefly Luc+, CmYLCV::STM::35S terminator, Nos::WUS2::PinII terminator
    Depositor
    Daniel Voytas
    Article
    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103
    Inserts
    CmYLCV::Luc+::AtHSP
    Nos::WUS2::PinII
    AtUbi10::STM::35S
    Use
    Luciferase; Gemini viral replicon, plant developm…
    Tags
    Expression
    Mutation
    Promoter
    AtUbi10, CmYLCV, and Nos
    Available since
    Nov. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    160696-plasmid-map-sequence-id-316629

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  26. pAAV-syn-flex-iGluSnFR4f-NGR-WPRE

    Plasmid
    #234442
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4f-NGR
    Use
    AAV and Cre/Lox
    Tags
    IgK chain and Myc epi tag-NGR GPI anchor
    Expression
    Mammalian
    Mutation
    Promoter
    Synapsin
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234442-plasmid-map-sequence-id-465693

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  27. pDRM210 LGP (Luciferase GFP Puro)

    Plasmid
    #174722
    Purpose
    Expresses the firefly luciferase gene, E2A, eGFP, F2A, and the puromycin resistance gene
    Depositor
    Wayne Tilley
    Article
    Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413
    Inserts
    Firefly Luciferase
    eGFP
    pac
    Use
    Lentiviral and Luciferase
    Tags
    E2A linker and F2A linker
    Expression
    Mammalian
    Mutation
    Promoter
    MND, MND (off Luc-E2A), and MND (off Luc-E2A-eGFP…
    Available since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    174722-plasmid-map-sequence-id-348963

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Showing: 3331 - 3360 of 159481 results