Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

Your Selections

Bacterial Expression

30,514 results

Showing: 1 - 20 of 30514 results

  5. EGFP-pBAD

    Plasmid
    #54762
    Purpose
    Localization: Protein Expression Vector , Excitation: 488, Emission: 507
    Depositor
    Michael Davidson
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    6xHis and EGFP
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54762-plasmid-map-sequence-id-212808

    Enlarge

  7. Tn7 integration plasmid

    Plasmid
    #154134
    Purpose
    Suicidal integration plasmid with the Tn7 arms, streptomycin and kanamycin resistance genes and the restriction site to clone the barcode-carrying cassette.
    Depositor
    Shimon Bershtein
    Article
    Jasinska et al Nat Ecol Evol. 2020 Feb 24. pii: 10.1038
    Inserts
    left and right end of Tn7 arm
    Spectinomycin resistant gene
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Spectinomycin resistance gene promoter and no
    Available Since
    July 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    154134-plasmid-map-sequence-id-306811

    Enlarge

  8. pBBR1MCS-2

    Plasmid
    #85168
    Purpose
    Mobilisable shuttle and expression vector. Replicates in many Gram-negative bacteria. Has multiple cloning site with blue/white selection function. Cloned genes driven by derepressed lac promoter.
    Depositor
    Kenneth Peterson
    Article
    Kovach et al Gene. 1995 Dec 1;166(1):175-6.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Constitutive (derepressed P-lac)
    Available Since
    Nov. 8, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    85168-plasmid-map-sequence-id-372970

    Enlarge

  10. pEF1-Pls3-sfGFP

    Plasmid
    #205750
    Purpose
    Expresses sfGFP under IPTG inducible promoter Pls3 in high copy pTRKH2 gram-positive shuttle vector. Used in Lactobacillus gasseri. Medium/High strength.
    Depositor
    Jerome Bonnet
    Article
    Fristot et al ACS Synth Biol. 2024 Mar 15;13(3):951-95
    Type
    Empty backbone
    Use
    Shuttle vector gram+ gram-
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pls3 (Phyperspank mutant, IPTG inducible)
    Available Since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    205750-plasmid-map-sequence-id-478438

    Enlarge

  11. pET28a MDHC_YEAST

    Plasmid
    #204232
    Purpose
    Bacterial expression of yeast cytosolic malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    MDHC_YEAST (MDH2 Budding Yeast)
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Promoter
    Lac
    Available Since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204232-plasmid-map-sequence-id-413337

    Enlarge

  12. pET28a MDHM_YEAST

    Plasmid
    #204231
    Purpose
    Bacterial expression of yeast mitochondrial malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    MDHM_YEAST (MDH1 Budding Yeast)
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Does not include transit peptide and includes a M…
    Promoter
    Lac
    Available Since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204231-plasmid-map-sequence-id-413335

    Enlarge

  13. pCRISPomyces-2BD

    Plasmid
    #234428
    Purpose
    Plasmid for Streptomyces containing gene of modified Cas9-BD protein and sequence of sgRNA cloning template
    Depositor
    Min-Kyu Oh
    Article
    Kim et al Nat Commun. 2025 Jan 21;16(1):874. doi:
    Insert
    Modified Cas9 with polyaspartate linking
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Linked polyaspartate using glycine-serine linker
    Promoter
    Available Since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    234428-plasmid-map-sequence-id-468848

    Enlarge

  15. SepRS(2)/pSertRNA(B4)/EF-Sep

    Plasmid
    #173897
    Purpose
    The plasmid contains an orthogonal aminoacyl-tRNA synthetase/tRNACUA pair, which directs the efficient incorporation of phosphoserine (pSer) into recombinant proteins in Escherichia coli.
    Depositor
    Jason W Chin
    Article
    Rogerson et al Nat Chem Biol. 2015 Jul;11(7):496-503. d
    Inserts
    SepRS(2)
    pSertRNA
    EF-Sep
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    173897-plasmid-map-sequence-id-342748

    Enlarge

  16. pKM512

    Plasmid
    #140192
    Purpose
    For curing of Orbit-integrated plasmids and associated drug marker
    Depositor
    Kenan Murphy
    Article
    Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-
    Inserts
    zeocin resistance gene
    Bxb1gp47 (directionality factor)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Cloned in gp47 directinality factor in place of R…
    Promoter
    EM7 and Ptet
    Available Since
    Jan. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    140192-plasmid-map-sequence-id-418051

    Enlarge

  19. pET His6 GST TEV LIC cloning vector (1G)

    Plasmid
    #29655
    Depositor
    Scott Gradia
    Type
    Empty backbone
    Use
    Tags
    6xHis, GST, and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available Since
    June 7, 2011
    Availability
    Academic Institutions and Nonprofits only
    29655-plasmid-map-sequence-id-196920

    Enlarge

Showing: 1 - 20 of 30514 results