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31,059 results

Showing: 3961 - 4000 of 31059 results

  5. pHTganA-Cpf1

    Plasmid
    #158647
    Purpose
    Constitutive transcription of FnCpf1 and ganA crRNA
    Depositor
    Wenjing Cui
    Insert
    Cpf1, ganA crRNA
    Use
    CRISPR and Synthetic Biology; Shuttle vector baci…
    Expression
    Bacterial
    Promoter
    P43
    Available Since
    Oct. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    158647-plasmid-map-sequence-id-314144

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  10. pSF18

    Plasmid
    #222593
    Purpose
    BB2 ZWF1 tet-on overexpression cassette
    Depositor
    Matthias Steiger
    Article
    Matthias et al PLOS One
    Insert
    ZWF1
    Expression
    Bacterial
    Mutation
    Internal BsaI and BpiI sites removed from insert
    Promoter
    tet-on
    Available Since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    222593-plasmid-map-sequence-id-443809

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  11. pLyGo-Bs-1

    Plasmid
    #163137
    Purpose
    pLyGo cloning vector for a sequence of interest (LPMO) in Bacillus subtilis. Vector encoding the AmyL signal peptide and the LyGo casette (SapI-ccdB-SapI)
    Depositor
    Morten Norholm
    Article
    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    PamyL-PamyQ-PcryIIIA-cryIIIAstab49
    Available Since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    163137-plasmid-map-sequence-id-332146

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  13. pET-29b(+)_His-CBD-3C-EmPNGaseF

    Plasmid
    #218193
    Purpose
    Bacterial expression of a His-tagged Chitin Binding fusion of Elizabethkingia miricola PNGaseF
    Depositor
    Florence Pojer
    Insert
    PNGaseF
    Tags
    tufA-His-CBD-3C
    Expression
    Bacterial
    Mutation
    codon optimized Ecoli, residues 41-354
    Promoter
    T7
    Available Since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    218193-plasmid-map-sequence-id-431798

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  15. pDM042

    Plasmid
    #216812
    Purpose
    Aspergillus nidulans codon-adjusted mScarlet3 fluorescent protein, includes linker for C-terminal tagging.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Insert
    mScarlet3
    Tags
    VinnyLinker
    Expression
    Bacterial
    Mutation
    Aspergillus nidulans codon-adjusted
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216812-plasmid-map-sequence-id-429538

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  16. pEM-Cas9HF1

    Plasmid
    #89961
    Purpose
    Modified from pCas9-CR4 (Addgene: 62655) to use the high-fidelity version of Cas9, SpCas9-HF1 (N497A/R661A/Q695A/Q926A) from Kleinstiver et al 2016.
    Depositor
    Michael Lynch
    Article
    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021
    Insert
    Cas9HF1
    Expression
    Bacterial
    Mutation
    N497A/R661A/Q695A/Q926A
    Promoter
    pTet
    Available Since
    June 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    89961-plasmid-map-sequence-id-181339

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  19. pZP06B

    Plasmid
    #232321
    Purpose
    Make sacB a counterselection marker to replace galK
    Depositor
    Chenggang Wu
    Insert
    PrpsJ-sacB
    Expression
    Bacterial
    Available Since
    March 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    232321-plasmid-map-sequence-id-462085

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  31. NRASA

    Plasmid
    #25256
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    NRASA:M1-N172 (NRAS Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids M1-N172
    Available Since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  34. FKBP1AA-c001

    Plasmid
    #53671
    Purpose
    bacterial expression plasmid
    Depositor
    Nicola Burgess-Brown
    Insert
    FKBP1AA
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    Bacterial expression for structure determination;…
    Available Since
    June 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  35. pBAD24-sfGFPx2

    Plasmid
    #51559
    Purpose
    Superfolder GFP ORF cloned into pBAD24 for expression in E. coli. It contains additional aminoacids in the N and C terminus (polilinker sequences for cloning proteins in frame with GFP)
    Depositor
    Sankar Adhya, Francisco Malagon
    Article
    Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261
    Insert
    superfolder GFP
    Tags
    GLESTCRHASLAVLADERRFSA and MARARA
    Expression
    Bacterial
    Mutation
    Contains additonal aa in the N and C terminus (in…
    Available Since
    March 26, 2014
    Availability
    Academic Institutions and Nonprofits only
    51559-plasmid-map-sequence-id-78831

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  36. pET-29b(+)_NSP4ss_SerM_NucA-myc

    Plasmid
    #218191
    Purpose
    Bacterial expression of a periplasmically expressed myc-tagged Serratia Marcescens NucA (Benzonase)
    Depositor
    Florence Pojer
    Article
    Rieubon et al protocols.io 2025
    Insert
    nucA
    Tags
    NSP4 signal secretion peptide and myc
    Expression
    Bacterial
    Mutation
    codon optimized ecoli residues - 22-266
    Promoter
    T7
    Available Since
    May 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    218191-plasmid-map-sequence-id-433865

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  39. pUS252

    Plasmid
    #127674
    Purpose
    Expresses fuGFP constitutively in E.coli cells. Designed to act as modular template for cutting out or amplifying fuGFP to place in other plasmid backbones
    Depositor
    Nicholas Coleman
    Insert
    Free Use GFP (fuGFP)
    Expression
    Bacterial
    Promoter
    lac
    Available Since
    June 24, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    127674-plasmid-map-sequence-id-251848

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Showing: 3961 - 4000 of 31059 results