30,738 results

1. pMH-MBP

   Plasmid

   #101768

   Purpose

   Gateway-compatible destination vector for bacterial expression of N-terminal MBP-tag fusion protein

   Depositor

   Michael Huen

   Article

   An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

   Type

   Empty backbone

   Tags

   MBP

   Expression

   Bacterial

   Available Since

   Oct. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSP6-RNAP

   Plasmid

   #48143

   Purpose

   Shuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage SP6 under control of the xylose inducible promoter PxylA

   Depositor

   Dieter Jahn

   Article

   Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

   Insert

   rna polymerase SP6
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   V314A (numbering based on NCBI Sequence ID: NP_85…

   Available Since

   Oct. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSJX022

   Plasmid

   #232325

   Purpose

   Genome editing in Caulobacter crescentus

   Depositor

   Wei Zhao

   Article

   Sun et al bioRxiv 2024.12.02.626314

   Inserts

   sgRNA
   SpCas9M
   

   Use

   CRISPR

   Tags

   H-arms and cat

   Expression

   Bacterial

   Promoter

   PJ23119 and Pvan

   Available Since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSJX016

   Plasmid

   #232324

   Purpose

   Genome editing in Caulobacter crescentus

   Depositor

   Wei Zhao

   Article

   Sun et al bioRxiv 2024.12.02.626314

   Inserts

   sgRNA
   SpCas9M
   

   Use

   CRISPR

   Tags

   H-arms and sfGFP

   Expression

   Bacterial

   Promoter

   PJ23119 and Pvan

   Available Since

   June 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pETDuet_sfCherry2(1-10)_sfCherry2(11)-SpyCatcher

   Plasmid

   #117656

   Purpose

   Bacterial expression vector for split sfCherry3 screening. The “sfCherry2(1-10)” and “sfCherry2(11)-SpyCatcher002” are expressed from two different T7 promoters.

   Depositor

   Bo Huang

   Article

   Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

   Inserts

   sfCherry2(1-10)
   sfCherry2(11)-SpyCatcher002
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. Opto-CRAC version 1

   Plasmid

   #101245

   Purpose

   Genetically-encoded calcium actuator; mCherry-tagged LOV2-STIM1 chimera that enables optical control of calcium signals and calcium dependent signaling

   Depositor

   Yubin Zhou

   Article

   He et al Elife. 2015 Dec 8;4. pii: e10024. doi: 1

   Insert

   LOV2 and STIM1 cytosolic fragment
   

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. PompF-cfp (ColE1)

   Plasmid

   #127948

   Purpose

   CFP fluorescent reporter for OmpF

   Depositor

   Mary Dunlop

   Article

   Rossi et al Commun Biol. 2019 Jul 11;2:259. doi: 10.

   Insert

   PompF-cfp
   

   Expression

   Bacterial

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. SpySwitch

   Plasmid

   #184225

   Purpose

   Expresses SpySwitch in the bacterial cytoplasm. SpySwitch binds SpyTag-, SpyTag002- or SpyTag003-fusions non-covalently for affinity purification, allowing gentle pH or temperature release.

   Depositor

   Mark Howarth

   Article

   Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

   Insert

   SpySwitch
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   E77A mutation prevents isopeptide bond formation,…

   Promoter

   T7

   Available Since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. 1a. pETM11-SUMO-SNCA-GFP

   Plasmid

   #107292

   Purpose

   Plasmid for the expression of the construct alpha-Synuclein-GFP, which contains N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubility

   Depositor

   Dmytro Yushchenko

   Article

   Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

   Insert

   SNCA-GFP (SNCA Human)
   

   Tags

   His-tag and SUMO-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAGM22082_sfGFP1-10

    Plasmid

    #153515

    Purpose

    E.coli expression plasmid for the production of GFP1-10

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Insert

    sfGFP1-10
    

    Expression

    Bacterial

    Available Since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDF RBP-FL P53

    Plasmid

    #174042

    Purpose

    Express Full length P53 in E.coli with cleavable expression/purification tag (ribose binding protein)

    Depositor

    Stewart Loh

    Article

    Blanden et al Elife. 2020 Dec 2;9. pii: 61487. doi: 10

    Insert

    P53 gene (TP53 Human)
    

    Tags

    Thermoanaerobacter Tencongenesis ribose binding p…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-28a (+)-hTBP

    Plasmid

    #171073

    Purpose

    Expresses human TBP in bacteria. The resulting plasmid was used to generate a single expression construct encoding a His-tagged TBP

    Depositor

    Joan Conaway

    Article

    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

    Insert

    TATA-box-binding protein (TBP Human)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMEXC3H

    Plasmid

    #110080

    Purpose

    Analogous to pMINTC3H, but containing oriM for episomal replication in mycobacteria.

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Type

    Empty backbone

    Tags

    3C cleavage site - His10 Tag

    Expression

    Bacterial

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOPINK-OTUD6B (full-length, aa 1-293)

    Plasmid

    #61418

    Purpose

    Expresses human OTUD6B (full-length) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD6B (OTUD6B Human)
    

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. p2CT-His-MBP-Lwa_Cas13a_WT

    Plasmid

    #91865

    Purpose

    Bacterial expression for Cas13a

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

    Insert

    LwaCas13a
    

    Use

    CRISPR

    Tags

    His6-MBP-TEV site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCFT4.2

    Plasmid

    #208807

    Purpose

    Expresses the fluorescent protein phiLOV2.1

    Depositor

    François-Xavier Campbell-Valois

    Article

    Tchagang et al Front Microbiol. 2023 Oct 20;14:1245755.

    Insert

    phiLOV2.1
    

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Oct. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBolux

    Plasmid

    #188109

    Purpose

    Encodes a promoter-less Bacteroides-optimized lux cassette

    Depositor

    Guy Townsend

    Article

    Modesto et al Nat Commun. 2023 Jan 17;14(1):275. doi:

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Jan. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRSFDuet1_CoiA1_CoiB

    Plasmid

    #208761

    Purpose

    Precursor peptide CoiA1 and synthase CoiB, for the co-expression of CoiA1 in E.coli.

    Depositor

    Wilfred van der Donk

    Article

    Luo et al Anal Chem. 2024 Jan 30;96(4):1767-1773.

    Inserts

    CoiA1
    CoiB
    

    Tags

    Hisx6 Tag and SUMO

    Expression

    Bacterial

    Mutation

    Changed Ser 20 to Asn, Changed Arg 84 to Lys, bot…

    Promoter

    T7

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOSIP-TT (TetR, P21)

    Plasmid

    #45989

    Depositor

    Keith Shearwin, Drew Endy

    Article

    St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTriEx4-Rac1-2G

    Plasmid

    #66110

    Purpose

    Second generation Rac1 FRET biosensor

    Depositor

    Olivier Pertz

    Article

    Fritz et al Dev Cell. 2015 Sep 29. pii: S1534-5807(1

    Insert

    Second generation Rac1 FRET biosensor
    

    Tags

    His-Myc

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    CMV

    Available Since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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