29,712 results

1. pGEX-5X-1-LARP7

   Plasmid

   #113545

   Purpose

   Produces an N-terminal GST-tagged full length LARP7 protein.

   Depositor

   Blerta Xhemalce

   Article

   Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

   Insert

   LARP7 (LARP7 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBP-lldR

   Plasmid

   #74095

   Purpose

   L-lactate repressor (LldR) from E. coli MG1655

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   lldR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   None

   Available since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLG024 (SIR2 + HerA)

   Plasmid

   #157902

   Purpose

   Expresses the SIR2 + HerA defense system

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2020 Aug 28;369(6507):1077-1084

   Insert

   SIR2 + HerA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p168-759

   Plasmid

   #62619

   Purpose

   ePathBrick pETM6 vector encoding CsF3H, AaDFR, DuLAR with Protein Scaffold GBD:SH3:PDZ (1:1:4)

   Depositor

   Mattheos Koffas

   Article

   Zhao et al Metab Eng. 2014 Dec 17;28C:43-53. doi: 1

   Inserts

   flavaone 3B-hydroxylase
   dihydroflavonol 4-reductase
   leucoanthocyanidin reductase
   Scaffold Protein encoding GBD:SH3:PDZ (1:1:4)
   

   Use

   Synthetic Biology

   Tags

   GBD, PDZ, and SH3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pKL003

   Plasmid

   #98919

   Purpose

   Constitutively expresses the voltage indicator PROPS under the Sigma 70 promoter in E. coli.

   Depositor

   Joel Kralj

   Article

   Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11

   Insert

   PROPS
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   BBa_J23118 - Strong Constitutive

   Available since

   Aug. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFW2500

   Plasmid

   #224213

   Purpose

   Bacteroides genomic editing tool; IPTG inducible-Fncpf1 and recT

   Depositor

   Ophelia Venturelli

   Article

   Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

   Insert

   Fncpf1 and recT
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pdCas9-J23109

   Plasmid

   #113151

   Purpose

   Plasmid constitutively expressing dCas9 protein

   Depositor

   Chong Zhang

   Article

   Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

   Insert

   dCas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D10A & H840A

   Promoter

   Available since

   Dec. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTU1-A-SP44-mScarlet-I

   Plasmid

   #163756

   Purpose

   Expresses Streptomyces codon-optimised mScarlet-I

   Depositor

   Paul Freemont, Simon Moore

   Article

   Moore et al ACS Synth Biol. 2021 Feb 19;10(2):402-41

   Insert

   mScarlet-I
   

   Use

   Synthetic Biology

   Tags

   Flag and FlAsH tags

   Expression

   Bacterial

   Mutation

   Promoter

   SP44

   Available since

   July 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRSET-WHaloCaMP1a

   Plasmid

   #205304

   Purpose

   Expression of WHaloCaMP1a in E. coli

   Depositor

   Eric Schreiter

   Article

   Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

   Insert

   WHaloCaMP1a
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBECKP-km

    Plasmid

    #117235

    Purpose

    A cytidine deaminase-mediated base-editing plasmid in Klebsiella Pneumoniae

    Depositor

    Quanjiang Ji

    Article

    Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

    Insert

    ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. MAC3-tag-N

    Plasmid

    #185482

    Purpose

    Gateway compatible destination vectors containing StrepIII/HA/UltralD

    Depositor

    Markku Varjosalo

    Article

    Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

    Type

    Empty backbone

    Use

    Tags

    StrepIII-HA-UltraID

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 3xFLAG-CUL1-pCMV7.1

    Plasmid

    #155019

    Purpose

    N-terminal 3xFLAG-tagged CUL1 in a pCMV7.1 vector

    Depositor

    Andre Catic

    Article

    Sweeney et al Sci Rep. 2020 Aug 18;10(1):13942. doi: 1

    Insert

    Cullin 1 (CUL1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Bacterial

    Mutation

    Promoter

    pCMV7.1

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCN38-yfp

    Plasmid

    #227637

    Purpose

    Negative control plasmid encoding yfp downstream of an attenuated ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and chloramphenicol resistance to S. aureus.

    Depositor

    Angelika Grundling

    Article

    Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

    Insert

    yfp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. MP-P

    Plasmid

    #69661

    Purpose

    Untargetted Bacterial Mutagenesis

    Depositor

    David Liu

    Article

    Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

    Insert

    dnaQ926 dam seqA emrR mutH(E56A)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E. coli PBAD

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PAJ698

    Plasmid

    #178947

    Purpose

    Expresses P2 tail fiber (gpH) in fusion with S16 tail fiber (gp37)

    Depositor

    Alfonso Jaramillo

    Article

    Cunliffe et al ACS Synth Biol. 2022 Oct 21;11(10):3207-

    Insert

    Chimeric Tail Fiber Fusion P2-gpH and S16 gp37
    

    Use

    Unspecified

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRSET-iGluSnFR4s

    Plasmid

    #234443

    Purpose

    Bacterial expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow)

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4s
    

    Use

    Tags

    6xHis-Xpress epi

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. NUDT16A

    Plasmid

    #42404

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    NUDT16A (NUDT16 Human)
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    See Comments

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pGEX/PD-L1 WT

    Plasmid

    #121466

    Purpose

    Expression of GST-human PD-L1 in E. Coli

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Insert

    CD274 (CD274 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJK635

    Plasmid

    #72473

    Purpose

    pET28a modified to contain TEV protease fusion tag instead of T7 tag

    Depositor

    T. J. Kappock

    Type

    Empty backbone

    Use

    Tags

    His6 and TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Sumo3 PolQM1

    Plasmid

    #78462

    Purpose

    Inducible expression in E. coli

    Depositor

    Susan Wallace, Sylvie Doublie

    Article

    Hogg et al J Mol Biol. 2011 Jan 21;405(3):642-52. d

    Insert

    Polymerase theta (POLQ Human)
    

    Use

    Tags

    His and SUMO3

    Expression

    Bacterial

    Mutation

    delta 1-1791; Q2513R (please see depositor commen…

    Promoter

    T7

    Available since

    July 28, 2016

    Availability

    Academic Institutions and Nonprofits only