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pET23a-mUbc9
Plasmid#53137PurposeE.coli expression of full length untagged mouse Ubc9 (protein sequence identical to human)DepositorInsertUbc9 (Ube2i Mouse)
UseTagsuntaggedExpressionBacterialMutationprotein sequence is identical to humanPromoterT7Available sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJKR-H-cdaR
Plasmid#62557PurposeGlucarate biosensor. High copy plasmid that expresses GFP proportional to glucarate concentration.DepositorInsertsbeta lactamase
sfGFP
CdaR
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
2NVR
Plasmid#51340PurposeCatalytic domain of human histone deacetylase HDAC7DepositorInsertHDAC7
UseTagsHisExpressionBacterialMutationTruncation: See CommentsPromoterT7Available sinceFeb. 12, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMRE161
Plasmid#118509Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertmTurquoise2
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLG018 (AVAST type 2)
Plasmid#157896PurposeExpresses the AVAST type 2 defense systemDepositorInsertAVAST type 2 (STAND ATPase with uncharacterized helical N-terminal domain)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
PZac2.1 gfaABC1D-Aqp4-eGFP
Plasmid#176747PurposeExpresses Aquaporin-4 fused with eGFP at the C-terminus in astrocytesDepositorInsertAqp4-eGFP
UseAAVTagsGFPExpressionBacterialMutationPromotergfaABC1DAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLP-pTac-Tn5-chlorR
Plasmid#199994PurposeLanding Pad with T7 RNAP circuit variant T15. No seed promoter. pTac-Tn5 transposase. In dap auxotrophic conjugation donor. This material contains 2 plasmids. Look below for more information.DepositorInsertLanding Pads
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPath-pTac-integrase
Plasmid#199999PurposeEntry vector for biosynthetic pathway. pTac-phiC31 integrase. This material contains 2 plasmids. For more information look under the "Depositor Comments" section below.DepositorInsertPathways
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQlinkH-PTPIP51(36-235)
Plasmid#170533PurposeExpresses Cleavable His-tagged PTPIP51_ΔTPR domain (36-235) in E. ColiDepositorInsertProtein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
UseTagsHis tagExpressionBacterialMutationPromoterT5 promoterAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTJV1ScKm-rpoB
Plasmid#166980PurposeGenerates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; npt2 for kanamycin resistanceDepositorInsertNeoR/KanR
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-RNAPII-CTD wt #14-HA
Plasmid#160688PurposeExpression of GST-RNA Polymerase II C-terminal domain fusion protein. Contains 14 of the consensus heptapeptide repeats shown to be phosphorylated by Cdc2 kinase.DepositorInsertRNA Polymerase II CTD
UseTagsExpressionBacterialMutationC terminal domain onlyPromoterAvailable sinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28b-FOXP2_P6
Plasmid#122649PurposeExpresses truncated variant (aa271-aa500) of human FOXP2 in bacterial cellsDepositorInsertHomo sapiens forkhead box P2 (FOXP2 Human)
UseTagsHis-tag and Strep-tag IIExpressionBacterialMutationPromoterT7Available sinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMUT2-VtrA-mCherry
Plasmid#192862PurposepMUT2 based vector for sensing human secondary bile acids in E. coli Nissle 1917DepositorInsertVtrA-CadC fusion protein and cognate promoter inducible by secondary bile acids
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCPP3416
Plasmid#128720PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopT1-1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCPP5534
Plasmid#128723PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopX1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEMTEZ-TeTxLC
Plasmid#32640DepositorInsertTetanus toxin light chain
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSLC-246
Plasmid#73190PurposeTemplate plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (tse2) under the control of rhamnose induceable promoter (PrhaB) for negative selection.DepositorInsertPrhaB-tse2
UseTagsExpressionBacterialMutationPromoterPrhaBAvailable sinceJuly 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET15bHis6Tnp
Plasmid#79807PurposeTransform BL21 Gold(DE3) to induce the expression of HisTnp hyperactive mutationsDepositorInsertHis6Tnp
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETM6-HpaBC
Plasmid#71260PurposeExpresses E. coli HpaBC in monocistronic form from ePathBrick vector, pETM6DepositorInsertsHpaB
HpaC
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOPINS3C
Plasmid#41115DepositorTypeEmpty backboneUseTagsExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceMarch 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRE107
Plasmid#43829DepositorTypeEmpty backboneUseBacterial allelic exchange vector with sacb1TagsExpressionBacterialMutationPromoterAvailable sinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSEVA421-Cas9tr
Plasmid#138709PurposePlasmid constitutively expressing the Cas9 nuclease and the non-coding tracrRNA from S. pyogenesDepositorInserttracrRNA-Cas9
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEaaK-NanR-J23113-pNanA-CadC-pCadBA-GFP
Plasmid#200277PurposeCharacterisation of the sialic acid-responsive circuit with fluorescent reporterDepositorInsertsCadC
NanR
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T3-GST-NQO1
Plasmid#177140PurposeBacterial vector for expression of an N-terminal GST fusion of NQO1 with a TEV protease site located between the GST tag and NQO1DepositorInsertNQO1 (NQO1 Human)
UseTagsGSTExpressionBacterialMutationPromoterTacAvailable sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046828
Plasmid#145742PurposeBacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pKB231
Plasmid#170018PurposeEncodes LacI-controlled expression of surface-displayed cecropin P1 and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
Cecropin P1
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialMutationPromoterPtac and PvirKAvailable sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKOR1-mcs
Plasmid#181755PurposeAllele replacement for Staphylococcus aureusDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pGEX6P-1-Dendra2
Plasmid#82436PurposeDendra2 expression plasmidDepositorInsertDendra2
UseTagsGST tagExpressionBacterialMutationPromotertacAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
RNF146-c005
Plasmid#173091PurposeExpression of recombinant fusion protein in E. coliDepositorInsertRNF146 (RNF146 Human)
UseTagsmCeruleanExpressionBacterialMutationR99-L183, codon optimizedPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pYDE009
Plasmid#194151PurposeDelivery of dcas9 into A. baumannii via Tn7 for CRISPRi. Tn7 element has tetR-tetP-dcas9, GmR; plasmid backbone is CarbR.DepositorInserttetR-tetP-dcas9-rrnB T1-T7Te
UseTagsExpressionBacterialMutationPromotertetpAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAC1430
Plasmid#127981PurposeAMA1 plasmid with Aspergillus optimized Cpf1, pyrG selection marker, and USER cassette (PacI/Nt.BbvCI)DepositorInsertCpf1
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationcodon optimized for Aspergillus nidulansPromoterAspergillus nidulans tef1 promoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDF_6xHIS_Csx29
Plasmid#197613PurposeFor protein purification of Csx29DepositorInsert6-HIS tagged Csx29
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKP864
Plasmid#167658PurposeExpress colicinB-His6DepositorInsertcba-his6
UseTagsExpressionBacterialMutationcba-his6PromoterAvailable sinceJuly 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmSod1-G93A-N’His
Plasmid#117705Purposemutation on stability/solubility of SOD1DepositorInsertSod1 (Sod1 Mouse)
UseTagsHis tag with Thrombin cleavage siteExpressionBacterialMutationG93APromoterT7Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
Affibody HER2:243.BirA*-pET301/NT-DEST
Plasmid#174692PurposePlasmid encoding for the bacterial expression of a bispecific coding for the anti-HER2 affibody ZHER2:342 fused to the mutated form of BirA enzyme, BirA*DepositorInsertAnti-HER2 affibody ZHER2:342 fused to mutated form of BirA, BirA*
UseTagsHIS tagExpressionBacterialMutationR118G mutation in BirAPromoterT7 promoterAvailable sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMT-ADARcd-E488Q-V5
Plasmid#139686PurposeExpresses only the hyperactive form of the ADAR catalytic domain - no RNA binding protein. This is a control plasmid to be used with pMT-Hrp48-ADARcd-E488Q-V5DepositorInsertAdenosine deaminase acting on RNA (Adar Fly)
UseTagsV5ExpressionBacterial and InsectMutationPromoterAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only
