32,115 results
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Plasmid#110248PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only
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pTSA-13
Plasmid#17327DepositorInsertstreptavidin
ExpressionBacterialMutationTruncated to include only amino acids 16-133 of m…Available SinceFeb. 25, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAIP4_3KB5
Plasmid#234225PurposeHeterologous protein expression of tripartite motif-containing protein 72 (Mg53) in Escherichia coliDepositorAvailable SinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTrcHisB-TRF2
Plasmid#50488PurposeE. Coli N-term HexHis Tagged Protein Expression VectorDepositorInsertTRF2 (TERF2 Human)
Tags6x His, T7 tag, and TEV protease siteExpressionBacterialMutationContains amino acids 3-500PromoterpTrcAvailable SinceFeb. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCR-II-TOPO-mitfa_LexPR-2A-Cerulean-SV40pA-FRT-Kan-FRT: Sequences,
Plasmid#110201PurposeMelanocyte-specific expression of the LexPR-2A-Cerulean fusionDepositorInsertLexPR transactivator - 2A - Cerulean
TagsCeruleanExpressionBacterialAvailable SinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMAT11
Plasmid#112592PurposeUsed for expression of protein in E. coli as Thrombin and TEV cleavableN-terminal 6His-tag-MBP fusions and without NcoI site within MBP.DepositorTypeEmpty backboneTags6His-MBPExpressionBacterialAvailable SinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
TJP1_PDZ_1
Plasmid#103909PurposeProtein expression and purification of ZO1 PDZ1 domainDepositorAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR11 WT NFAT1
Plasmid#11791DepositorAvailable SinceMay 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
ecALDH
Plasmid#67921PurposeExpresses aldehyde dehydrogenase from Escherichia coli, codon optimized for expression in E. coliDepositorInsertEscherichia coli aldehyde dehydrogenase (puuC Escherichia coli)
Tags6x-HisExpressionBacterialPromoterT7Available SinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCoofy1
Plasmid#43974DepositorTypeEmpty backboneTagsHis6ExpressionBacterialPromoterT7Available SinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pOGG202
Plasmid#115503PurposeClone of a level 1 unit which encodes IPTG-inducible sfGFPDepositorInsertpL1M-F1-plac pOGG031, sfGFP pOGG037, T-pharma pOGG003
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
L4440_BioBrick-hif-1-sgRNA-B
Plasmid#177785PurposeOverexpression of sgRNAs in E. coli HT115 (targeting C. elegans hif-1 promoter)DepositorInserthif-1 (hif-1 Nematode)
ExpressionBacterialAvailable SinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAD-D2
Plasmid#37470DepositorInsertD2 second generation cameleon (calcium sensor)
ExpressionBacterialPromoterAraAvailable SinceOct. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pF420
Plasmid#123659PurposeProduces cofactor F420 in E. coliDepositorInsertsCofD
FbiB
FbiD
FbiC
UseSynthetic BiologyExpressionBacterialPromoterBBa_R0040Available SinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
RCAS(B)-Rheb-Q64L
Plasmid#21050DepositorInsertRas-homolog enriched in brain (RHEB Human)
UseRetroviralTagsFLAGExpressionBacterialMutationglutamine 64 to leucineAvailable SinceAug. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-hph-dCas9
Plasmid#218371PurposeExpression of dCas9 for CRISPR-interferenceDepositorInsertdCas9
UseCRISPRExpressionBacterialPromoterPtetAAvailable SinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21a-C2-mKate
Plasmid#208763PurposeTo express C2-mKate in bacteriaDepositorAvailable SinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTYB1-eIF4A
Plasmid#37225DepositorInserteIF4A
TagsinteinExpressionBacterialPromoterT7Available SinceJuly 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET24b-Amg2
Plasmid#199434Purposeexpresses Amg2 in bacterial cellsDepositorInsertAmg2
Tagshis tagExpressionBacterialMutationL159K mutationPromoterT7Available SinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWN172
Plasmid#215291PurposeExpresses sfGFP under the Anderson J23113 promoter with an RSF1010 backbone.DepositorInsertsfGFP
TagsHisExpressionBacterialPromoterJ23113Available SinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBS FGF3 isoform 1
Plasmid#19230DepositorInsertFibroblast growth factor receptor 3 isoform 1 (FGFR3 Chicken)
ExpressionBacterialAvailable SinceSept. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-RhoA FLARE.sc Biosensor T19N
Plasmid#12152DepositorInsertRhoA Biosensor T19N (RHOA Human)
Tags6xHisExpressionBacterial, Insect, and Mamm…MutationRhoA T19N (dominant-negative mutation)*Available SinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGRG37
Plasmid#16667DepositorInsertmTn7::Gateway cassette
ExpressionBacterialAvailable SinceJan. 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
pETM6-G6-vioABE
Plasmid#73438PurposeProdeoxyviolacein pathway (vioABE) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variant G6.DepositorInsertPG6-vioABE
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterPG6 (orthogonal T7-lac variant)Available SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMT316
Plasmid#8608DepositorInsertbarstar
ExpressionBacterialAvailable SinceJan. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pTlpA_mCherry_YafQ/DinJ
Plasmid#229148PurposeToxin-Antitoxin (Dinj/YafQ)DepositorInsertmCherry, DinJ/YafQ
ExpressionBacterialPromoterPtlpA (mCherry), DinJ/YafQ (Native)Available SinceFeb. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
REM1
Plasmid#55681Purposeexpression of mouse REM1DepositorAvailable SinceSept. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
mEos3-dnRac1
Plasmid#92040PurposeExpresses mEos3 fusion with dnRac1 (T17N).DepositorInsertRAC1 (RAC1 Human)
TagsmEos3ExpressionBacterial, Insect, and Mamm…MutationT17N (dominant negative)PromoterCMVAvailable SinceJune 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
Pet22b-YAP
Plasmid#166443PurposeBacterial expression of 6x His tagged YAPDepositorInsertYAP (YAP1 Human)
ExpressionBacterialAvailable SinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET20b_Pa-PiuA
Plasmid#128955Purposeexpresses Pseudomonas aeruginosa PiuA in E. coliDepositorInsertiron transport outer membrane receptor (PiuA) (PA4514 Pseudomonas aeruginosa PAO1)
Tags6xHis and pelB leaderExpressionBacterialMutationSignal peptide sequence has been removedPromoterT7Available SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTSara
Plasmid#60720PurposeExpresses both fragments of T7 RNAP split at position 179. Both fragments are driven by the arabinose inducible promoter PBAD. Also contains a constitutive araC ORFDepositorInsertsN Terminal fragment of T7 RNAP split betwen 179-180
N Terminal fragment of T7 RNAP split betwen 179-180
ExpressionBacterialMutationT7 RNAP split between positions 179-180 and wt T7…PromoterPBADAvailable SinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
yTREX
Plasmid#177306Purposegenomic integration of genes (inserted in I-SceI-site) via transposon Tn5DepositorTypeEmpty backboneUseSynthetic Biology; Yeast expression, tn5 genomic …ExpressionBacterialAvailable SinceAug. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLibacceptorV2
Plasmid#106250PurposeRecombination-mediated cassette exchange with target genomic lociDepositorTypeEmpty backboneUseCre/Lox and Synthetic BiologyExpressionBacterialAvailable SinceNov. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
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NRASA
Plasmid#25256PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertNRASA:M1-N172 (NRAS Human)
TagsHisExpressionBacterialMutationContains amino acids M1-N172Available SinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTGV-Express-CAT SMR5916
Plasmid#28039DepositorTypeEmpty backboneExpressionBacterialAvailable SinceMarch 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-TEV-mDvl2PDZ(264-353)_pCDFduet
Plasmid#216386PurposeBacterial expression vector for MBP-tagged mouse Dvl2 PDZ domain (residues 264-353)DepositorAvailable SinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHis17_ParM _2
Plasmid#78202PurposeExpression of double-cysteine variant of plasmid segregation protein, ParM, as scaffold for ADP biosensorDepositorInsertparM (K33A D63C T174A T175N D224C C287A)
TagsHis6ExpressionBacterialMutationK33A D63C T174A T175N D224C C287APromoterunknownAvailable SinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
PdnaK GFP pZa
Plasmid#170073PurposeGFP expression under the control of E. coli dnaK promoterDepositorInsertGreen fluorescent protein
UseSynthetic BiologyExpressionBacterialPromoterPdnaKAvailable SinceSept. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCCL-GKL1RC-Short-LEUg1mKateg2GKL2ORF10UCS
Plasmid#52345PurposeIntegration cassette to create p1-Short-Leu-mKate with p2 ORF10 UCS>mKateDepositorInsertmKate2
UseP1 integration vectorExpressionBacterialMutationchanged V2HPromoterpGKL2 ORF10 UCSAvailable SinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only