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31,059 results

Showing: 5441 - 5480 of 31059 results

  2. pGBW-m4046785

    Plasmid
    #145684
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 N (nucleocapsid)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 N (nucleocapsid) (N Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;StrepII
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145684-plasmid-map-sequence-id-297841

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  7. pThermeleon

    Plasmid
    #86120
    Purpose
    Bandpass temperature-gated expression of mWasabi and mCherry reporters
    Depositor
    Mikhail Shapiro
    Article
    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi
    Inserts
    TlpA
    mWasabi
    TcI38
    mCherry
    cI
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    A67T, M1V, L65S, K68R, F115L, D126G, D188G and D3…
    Promoter
    pR/pL, pR/pL, pLacI, and pTlpA
    Available Since
    March 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    86120-plasmid-map-sequence-id-173201

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  9. pDEST14-SpyCatcher003-TEVs-SpyTag003DA

    Plasmid
    #216283
    Purpose
    Expresses SpyCatcher003-TEV site-SpyTag003DA in bacterial cytoplasm, to allow TEV protease to remove SpyTag003DA and thereby uncage SpyCatcher003 reactivity.
    Depositor
    Mark Howarth
    Article
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    SpyCatcher003-TEVs-SpyTag003DA
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    216283-plasmid-map-sequence-id-433206

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  11. pJL31

    Plasmid
    #227428
    Purpose
    Kanamycin resistant L5-integrative vector for CRISPR interference in M.smegmatis with constitutive expression of mWasabi under the control of the psmyc promotor (psmyc::mWasabi)
    Depositor
    Pierre Santucci
    Article
    Laudouze et al FEBS Lett. 2025 Feb;599(4):488-501. doi:
    Insert
    mWasabi
    Use
    CRISPR
    Expression
    Bacterial
    Available Since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    227428-plasmid-map-sequence-id-455967

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  12. pJL32

    Plasmid
    #227429
    Purpose
    Kanamycin resistant L5-integrative vector for CRISPR interference in M.smegmatis with the constitutive expression of tdTomato under the control of the psmyc promotor (psmyc::tdTomato)
    Depositor
    Pierre Santucci
    Article
    Laudouze et al FEBS Lett. 2025 Feb;599(4):488-501. doi:
    Insert
    tdTomato
    Use
    CRISPR
    Expression
    Bacterial
    Available Since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    227429-plasmid-map-sequence-id-455968

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  18. pFS_0414_pET30_MCS-BBaJ23100-LuxR-B0015term-pLux rR-Fluc-rrnBterm-pCat-tetR-Term-ptetA-Rluc-rrnBterm_FsRT-Cas1-Cas2_ARRAY2_FaqI

    Plasmid
    #117005
    Purpose
    Expresses FsRT-Cas1-Cas2 under pT7lac, Fluc under pLuxR and Rluc under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout
    Depositor
    Randall Platt
    Article
    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:
    Insert
    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Nov. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    117005-plasmid-map-sequence-id-227039

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  20. BRD2

    Plasmid
    #39074
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    BRD2 (BRD2 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  26. pGR618

    Plasmid
    #213786
    Purpose
    Integrates at the CAN1 locus the BB-Tv TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette
    Depositor
    Chang Liu
    Insert
    BB-Tv TP-DNAP1
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available Since
    March 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    213786-plasmid-map-sequence-id-424624

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  27. pETDest

    Plasmid
    #124245
    Purpose
    Allows Gateway cloning into the bacterial 6xHIS tagged protein expression vector pET26
    Depositor
    Attila Fehér
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    Sept. 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    124245-plasmid-map-sequence-id-257540

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  29. pRN3P-NLS-EGFP-Btgh-3NLS

    Plasmid
    #194469
    Purpose
    Encodes for bacterial O-GlcNAcase BtGH84 fused to enhanced GFP and NLSs for effective nuclear localization, in backbone for T3 In Vitro Transcription.
    Depositor
    Mathieu Boulard
    Article
    Formichetti et al Dev Cell 10 Jan 2024
    Insert
    BtGH84
    Use
    For in vitro transcription from t3 promoter after…
    Tags
    SV40 Nuclear Localization Signal, SV40 Nuclear Lo…
    Expression
    Bacterial
    Available Since
    Jan. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    194469-plasmid-map-sequence-id-384873

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  30. pBAD-mLemon

    Plasmid
    #191256
    Purpose
    Bacterial expression plasmids for pH sensitive fluorescent protein mLemon
    Depositor
    Robert Campbell
    Insert
    mLemon
    Tags
    His tag
    Expression
    Bacterial
    Available Since
    Nov. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    191256-plasmid-map-sequence-id-378486

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  33. pJA5 H-2K(b)

    Plasmid
    #180441
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    H-2K(b)
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Available Since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    180441-plasmid-map-sequence-id-355754

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  36. pKD‐SepRS-­EFSep-­5x tRNASep (B40 OTS)

    Plasmid
    #52054
    Purpose
    Used in phosphoprotein synthesis. Expresses the Sep-accepting tRNA (tRNASep), the Sep-tRNA synthetase (SepRS) and an engineered EF-Tu (EFSep)
    Depositor
    Jesse Rinehart
    Inserts
    SepRS
    EF-Sep
    5x tRNA Sep
    Expression
    Bacterial
    Mutation
    His67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…
    Promoter
    Ptac
    Available Since
    March 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    52054-plasmid-map-sequence-id-438919

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  40. pOPS0379

    Plasmid
    #115510
    Purpose
    Suitable for experiments in environments where there is no antibiotic selection present. Symbiosis-induced nifH promoter from Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing sfGFP
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    Rlv3841pNifH(pOGG082) sfGFP (pOGG037) and T-pharma (pOGG003) assembled in pOGG026
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Available Since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    115510-plasmid-map-sequence-id-224287

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Showing: 5441 - 5480 of 31059 results