29,771 results
-
Plasmid#199374PurposeEncodes dual cistronic nuclear localized jGCaMP7s and mScarlet-I optimized for C. elegans expressionDepositorInsertNLS::jGCaMP7s::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
UseTagsExpressionBacterialMutationno mutations, except added subcellular localizati…PromoterAvailable sinceApril 25, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPbU6_hdhfr/yfcu_Cas9
Plasmid#216423PurposeEmpty backbone to express gene specific gRNA from the Plasmodium berghei U6 promoter and the Cas9 nuclease for traditional CRISPR editing.DepositorTypeEmpty backboneUseCRISPR; Expression in plasmodium bergheiTagsExpressionBacterialMutationPromoterAvailable sinceApril 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
-
SRpHi4
Plasmid#102544PurposeBacterial expression of EYFP fused to Epidermal Growth Factor with a GST tag for purificationDepositorInsertEYFP-EGF
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
-
R989-X28-566: His6-MBP-tev-G-Hs.KRAS4b(1-188) G12D
Plasmid#159548PurposeE. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G12DDepositorInsertHis6-MBP-tev-G-Hs.KRAS4b(1-188) G12D (KRAS Human)
UseTagsHis6-MBP-tevExpressionBacterialMutationG12DPromoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Neo-PuroDTK
Plasmid#84036PurposePiggyBac cassette contains Neomycin, puromycin and TK genesDepositorTypeEmpty backboneUseTagsExpressionBacterial and MammalianMutationPromoterCAG promoterAvailable sinceApril 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pC029 - Lw2Cas13a from Leptotrichia wadei F0279
Plasmid#91919PurposeExpresses Lw2Cas13a for bacterial expression and contains backbone for spacer cloningDepositorInsertLw2Cas13a and guide expression cassette
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
MBP-FUS_LC_12E
Plasmid#98654PurposeExpresses MBP-tagged phosphomimetic FUS AA 1-163DepositorInsertFUS (FUS Human)
UseTags6xHis-MBPExpressionBacterialMutation12 S/T to EPromoterAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_YWHAZ_14-3-3
Plasmid#109808PurposeProtein expression and purification of YWHAZ_14-3-3DepositorInsertYWHAZ_14-3-3 (YWHAZ Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFPV25
Plasmid#20667DepositorTypeEmpty backboneUseTagsGFP mut3ExpressionBacterialMutationPromoterAvailable sinceMarch 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pNR291
Plasmid#79555PurposeOutput plasmid: contains 5-state register that regulates expression of GFP, RFP, and BFP. Expresses a unique fluorescent signature in each state.DepositorInsertsGFPmut3b
mTagBFP
mRFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pATetO 6XHis-MMLV RT
Plasmid#178797PurposeInducible expression of MMLV RT in E. coliDepositorInsertMMLV RT
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEThT-SAHS2
Plasmid#90026PurposeTo express His-tagged SAHS2 in bacteriaDepositorInsertSAHS2
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
p3521 pET ubcH6
Plasmid#8653DepositorInsertUbcH6 (UBE2E1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pHLUK
Plasmid#64181Purposeharbours marker genes HIS3, LEU2, URA3 and LYS2DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRB117.2
Plasmid#181949PurposeaTc-inducible expression of NarL with N-terminal mNeonGreen fusion. Also contains mCherry under NarL-controlled promoter PdcuSDepositorInsertUseTagsmNeonGreenExpressionBacterialMutationSee Depositor Comments BelowPromotermNG-NarL-PLtetO-1; mCherry-PdcuS; tetR-J23106Available sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGGB_RT_tdTomato_li
Plasmid#136964PurposeGoldengate/Greengate N-tag module, containing tdTomato fused to a flexible linkerDepositorInserttandem Tomato
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJOE3075.3
Plasmid#135084PurposeL-rhamnose-inducible expression vectorDepositorInsertP-rhaP-BAD-malE
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
3S8P
Plasmid#51360DepositorInsert3S8P
UseTagsHisExpressionBacterialMutationTruncation: See CommentsPromoterT7Available sinceFeb. 12, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSUMO His6 SUMO PYL1 (36-211)
Plasmid#177843PurposeBacterial Expression of PYL1DepositorInsertPYR1-like 1 (PYL1 Mustard Weed)
UseTags6xHis-SUMOExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS mouse c-Myc
Plasmid#14972DepositorInsertc-Myc (Myc Mouse)
UseTagsExpressionBacterialMutationNucleotides 2578-3294PromoterAvailable sinceMay 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET28a-IMX-DogTag
Plasmid#113765PurposeExpresses IMX-DogTag in bacterial cells for modular antigen multimerization using SnoopLigaseDepositorInsertIMX-DogTag
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHYDuet183
Plasmid#41685DepositorInsertPhoRadA intein
UseTagsGB1 and His-tagExpressionBacterialMutationPromoterT7Available sinceJan. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pETDuet RagA(D130N)-RagC
Plasmid#99642PurposeBacterial expression of RagA(D130N)-RagCDepositorUseTagsHis tagExpressionBacterialMutationD130NPromoterAvailable sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDSG287
Plasmid#115606Purposesurface-displayed Ag2, used in composability experiments; low copyDepositorInsertregulated adhesin construct
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
-
pGEX-Ubc13
Plasmid#18894DepositorInsertUbiquitin conjugating enzyme UBC13 (UBC13 Budding Yeast)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceSept. 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMP4657
Plasmid#107773PurposeExpression of EGFPDepositorInsertpLacZ-EGFP in pME6010
UseTagspLacZ-EGFPExpressionBacterialMutationPromoterpLacZAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS-rpoN-cIopt-J23106-geneIII
Plasmid#80852PurposePhagemid (encodes M13 gene III and lambda cIopt).DepositorInsertsM13 gene III
Lambda cIopt
UseTagsExpressionBacterialMutationPromoterBBa_J23106 and rpoNAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-P_vanR-PVanCC
Plasmid#204078PurposeType 0 promoter partDepositorInsertvanR repressor and PVanCC vanillic acid-inducible promoter
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAGE2.0-i
Plasmid#194639PurposeEmpty pAGE2.0 plasmid with pBAD inducible promoterDepositorTypeEmpty backboneUseSynthetic Biology; Diatom expressionTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD/CURT_fluoA
Plasmid#117998PurposeExpression of CURT_fluoA controlled by the ARA promoterDepositorInsertCURT_fluoA
UseTagsExpressionBacterialMutationPromoterARAAvailable sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
MP-S2
Plasmid#69691PurposeUntargetted Bacterial MutagenesisDepositorInsertdnaQ926 AAG(Y127I-H136L)
UseTagsExpressionBacterialMutationPromoterE. coli PBADAvailable sinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
MP-S3
Plasmid#69692PurposeUntargetted Bacterial MutagenesisDepositorInsertdnaQ926 d80-AAG(Y127I-H136L)
UseTagsExpressionBacterialMutationPromoterE. coli PBADAvailable sinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pK27Sumo_His-SUMO-nsp14-nsp10 fusion (SARS-CoV-2)
Plasmid#169160PurposeTo express nsp14-nsp10 fusion protein in E. coliDepositorInsert14His-SUMO-nsp14-GGSGGS-nsp10 (ORF1ab Synthetic, SARS-CoV-2)
UseTags14His-SUMO and Fusion protein of SARS-CoV-2 nsp14…ExpressionBacterialMutationCodon optimised for E. coliPromoterT5Available sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
pDEST17-UbE2E3
Plasmid#15789DepositorInsertUbiquitin conjugating enzyme UbE2E3 (UBE2E3 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
-
pAJT248
Plasmid#202289PurposeRPU integration plasmid (LPattB2)DepositorInsertJ23101-RiboJ00-B0034-YFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET151-Vps4
Plasmid#87733PurposeBacterial expression of full-length S. cerevisiae Vps4DepositorInsertVacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
UseTags6xHis-V5-TEVExpressionBacterialMutationPromoterT7Available sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
K560GFP_17NL
Plasmid#129767PurposeExpresses Drosophila Kinesin-1 with extended neck linker (DAL insert) truncated at 560 and GFP tagged for motility assaysDepositorInsertKHC (Khc Fly)
UseTagsGFP and His6ExpressionBacterialMutationDAL insert before A345PromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIBB-mEGFP-IRES-puro2b
Plasmid#26462DepositorInsertpIBB
UseTagsmEGFPExpressionBacterial and MammalianMutationPromoterAvailable sinceDec. 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1-DmPABPC1_J
Plasmid#146647PurposeBacterial Expression of DmPABPC1DepositorInsertDmPABPC1 (pAbp Fly)
UseTagsExpressionBacterialMutationG26S relative to NM_166265.2/NP_725750.1PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSM040
Plasmid#128294Purposeasymmetric plasmid partitioning E. coli - target plasmidDepositorInsertPiq - mRFP, parS
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnfΔpvc15_pvc12-HiBiT
Plasmid#198309PurposeNegative control for payload loading assay; produces PVCs deficient for payload loader (pvc15)DepositorInsertPVCpnfΔpvc15_pvc12-HiBiT
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
-
pBAD18-Gm
Plasmid#65100Purposebacterial expression vector with Gentamycin selection, based on pBAD18DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterpBADAvailable sinceJune 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
human p53-(82-360)
Plasmid#24868DepositorInsertp53 (TP53 Human)
UseTagsHisExpressionBacterialMutationContains aa 82-360PromoterAvailable sinceAug. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBbB19a-GFP
Plasmid#126131PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertGFPuv
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23105Available sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only