Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

Your Selections

Bacterial Expression

29,771 results

Showing: 8101 - 8150 of 29771 results

  6. KCC2G_HUMAN_D0

    Plasmid
    #79711
    Purpose
    This plasmid encodes the kinase domain of KCC2G. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
    Depositor
    John Chodera, Nicholas Levinson, Markus Seeliger
    Article
    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/
    Insert
    KCC2G (CAMK2G Human)
    Use
    Tags
    His10-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  8. rpoDs366C

    Plasmid
    #129688
    Purpose
    Expression of Sigma 70 S366C
    Depositor
    Jeff Gelles
    Article
    Mumm et al bioRxiv 2020
    Insert
    rpoD (rpoD E. coli)
    Use
    Tags
    His6/thrombin
    Expression
    Bacterial
    Mutation
    C132S C291S C295S S366C
    Promoter
    Available since
    May 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    129688-plasmid-map-sequence-id-270569

    Enlarge

  11. pET15b-sfGO-Matryoshka-LE-LP

    Plasmid
    #100018
    Purpose
    Bacterial expression of fluorescent protein casette - cpsfGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkers
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    cpsfGFP-LSSmOrange
    Use
    Tags
    6x HIS tag
    Expression
    Bacterial
    Mutation
    Leu and Glu N-term linker and Leu and Pro C-term …
    Promoter
    T7
    Available since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100018-plasmid-map-sequence-id-194292

    Enlarge

  15. pShCAST(Cas12k-TniQ-TniQ)-sgRNA_entry (CJT12)

    Plasmid
    #181788
    Purpose
    Expresses 3-component ShCAST containing a Cas12k-TniQ-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    ShTnsB, ShTnsC, ShCas12k-ShTniQ-ShTniQ
    Use
    Tags
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    181788-plasmid-map-sequence-id-354819

    Enlarge

  21. pTXB1-EXO1b D173A

    Plasmid
    #68269
    Purpose
    Bacterial expression of human EXO1 D173A (catalytically-dead) mutant
    Depositor
    Stefano Ferrari
    Article
    Eid et al EMBO Rep. 2010 Dec;11(12):962-8. doi: 10
    Insert
    EXO1 (EXO1 Human)
    Use
    Tags
    Mxe intein/chitin binding domain
    Expression
    Bacterial
    Mutation
    catalytically-dead D173A. Please see depositor co…
    Promoter
    T7
    Available since
    Aug. 12, 2015
    Availability
    Academic Institutions and Nonprofits only

  24. pET28a-TGFα-GSY-SPM

    Plasmid
    #163615
    Purpose
    Expresses mature human Transforming Growth Factor alpha fused to SPM in bacterial cells for calcium-induced covalent coupling to Epidermal Growth Factor Receptor.
    Depositor
    Mark Howarth
    Article
    Scheu et al Nat Commun. 2021 Jan 29;12(1):717. doi:
    Insert
    TGFa-GSY-SPM (TGFA Synthetic, Human)
    Use
    Tags
    C-tag and His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    163615-plasmid-map-sequence-id-323634

    Enlarge

  25. pOPS0750

    Plasmid
    #115511
    Purpose
    Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing gusA.
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    gusA pOGG083, T-pharma pOGG003 assembled in pOGG026
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Promoter
    Constitutive promoter pNeo pOGG001
    Available since
    Jan. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    115511-plasmid-map-sequence-id-223075

    Enlarge

  26. pCRISPReporter

    Plasmid
    #65007
    Purpose
    E. coli reporter vector with MCS for inserting bacterial promoter region through 5' end of gene of interest, upstream and in-frame with reporter cloning site
    Depositor
    Mattheos Koffas
    Article
    Cress et al ACS Synth Biol. 2015 Mar 30.
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    none
    Available since
    May 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    65007-plasmid-map-sequence-id-109898

    Enlarge

  30. pCosSAD1AprR

    Plasmid
    #190786
    Purpose
    An E. coli cloning vector for the integration of heterologous genes into a linear plasmid pSA3239 in Streptomyces lavendulae subsp. lavendulae CCM 3239 through homologous recombination
    Depositor
    Jan Kormanec
    Article
    Novakova et al Appl Microbiol Biotechnol. 2022 Nov;106(
    Inserts
    upstream region of the aur1 cluster for auricin
    ampramycin resistance cassette
    downstream region of the aur1 cluster for auricin
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    190786-plasmid-map-sequence-id-375582

    Enlarge

  31. SRM

    Plasmid
    #25490
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SRM (SRM Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  34. RAB3B (3DZ8)

    Plasmid
    #32879
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    RAB3B (RAB3B Human)
    Use
    Tags
    His tag (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    amino acids 18-190 only
    Promoter
    Available since
    Jan. 11, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  36. pBEAST_J23101-bphC

    Plasmid
    #128137
    Purpose
    The cell-free adapted backbone, pBEAST, expressing bphC (the enzyme converting biphenyl-2,3-diol to 2-hydroxy-6-oxo-6-phenylhexa-2,4-) under control of the constitutive promoter J23101, and RBS B0032
    Depositor
    Jean-Loup Faulon
    Article
    Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:
    Insert
    bphC
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23101
    Available since
    Aug. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    128137-plasmid-map-sequence-id-252649

    Enlarge

  39. pGBK CUP1 mGrape3

    Plasmid
    #170265
    Purpose
    Yeast two-hybrid vector using a copper inducible CUP1 promoter, expressing mGrape3
    Depositor
    Bruce Hamilton
    Article
    Hernandez et al bioRxiv
    Insert
    momeric Grape 3
    Use
    Tags
    GAL4 DNA Binding Domain and Myc
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    CUP1
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    170265-plasmid-map-sequence-id-335101

    Enlarge

  43. KHK

    Plasmid
    #25532
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    KHK (KHK Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 24, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 8101 - 8150 of 29771 results