-
Plasmid#23342DepositorInsertGFP(LVA)
UseTagsGFP(LVA)ExpressionBacterialMutationPromoterAvailable sinceApril 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
HC89 pComb3xss anti-Homer1 nanobody
Plasmid#134719PurposeBacterial expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an immunolabelDepositorInsertanti-Homer1 (Mouse) recombinant llama nanobody HC89 (Homer1 L. glama (llama))
UseTags6xHis-HAExpressionBacterialMutationPromoterlacAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
-
pBZCas13b-HEPN
Plasmid#89901PurposeBacterial expression for bzCas13b and crRNA with both HEPN domains mutated. New spacers can be cloned by digesting with BsaI.DepositorInsertCas13b
UseTagsExpressionBacterialMutationR116A/H121A/R1177A/H1182APromoterLacAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmRFPmars
Plasmid#26252DepositorInsertmRFPmars
UseTags6xHisExpressionBacterialMutationKpnI restriction site removedPromoterAvailable sinceOct. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pHis-zfCSF3a
Plasmid#65612Purposeexpress soluble His-tagged zebrafish CSF3a/Gcsfa in E. coliDepositorInsertCsf3a (csf3a Zebrafish)
UseTags(His)6ExpressionBacterialMutationPromoterT7Available sinceSept. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pReporter_5
Plasmid#60566PurposeA reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).DepositorInsertReporter_5
UseTagsExpressionBacterialMutationPromoterBBa_J23119Available sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-hOPTN
Plasmid#23053DepositorInsertoptineurin (OPTN Human)
UseTagsHisExpressionBacterialMutationN/APromoterAvailable sinceMay 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-p19-T7-Vif
Plasmid#46313DepositorInsertsp19
Vif DNA-1
linker
Vif DNA-2
UsePro-sirnaTags6XHis and GSTExpressionBacterialMutationPromoterAvailable sinceJan. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pQacR-Q1
Plasmid#74689PurposeQacR-Q1 gateDepositorInsertPTac-RBSQ1-QacR-PQacR-YFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS hIhh (CT#402)
Plasmid#13995DepositorInsertIhh (IHH Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMCSG7-dNmeCas9
Plasmid#71475Purposebacterial pMCSG7 expression vector expressing catalytically inactive Nme cas9 with T7 promoter, N-terminal His tag and TEV siteDepositorInsertpMCSG7-Inactive-NmeCas9
UseTags6HisExpressionBacterialMutationD16A H588APromoterAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1-Cdc42 G15A
Plasmid#69356Purposeexpression of nucleotide free Cdc42 in bacterial cellsDepositorInsertCdc42 (CDC42 Human)
UseTagsGSTExpressionBacterialMutationG15APromotertacAvailable sinceSept. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQadd2
Plasmid#135662PurposeClostridial vector, carrying lox511/66 and loxFAS/71 sites separated by restriction sites in order to add cargo. Use in combination with pQcre1 to insert cargo into lox sites, inserted with pQadd1F/R.DepositorInsertLox511/66 and LoxFas/71
UseClostridial vector, pamβ1 originTagsExpressionBacterialMutationPromoterAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRO16Sh2
Plasmid#180604PurposeExpression of ShCAST under control of Plac with pRO1600 origin.DepositorInsertShTnsB, ShTnsC, ShTniQ, ShCas12k
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpLacAvailable sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSG81-pLas-tetO-deGFPssrA
Plasmid#102454PurposeExpresses deGFP fluorescent protein with ssrA degradation tag in E. coli. The expression is controlled by a pLas-tetO combinatorial promoter.DepositorInsertdeGFP
UseTagsssrAExpressionBacterialMutationPromoterpLas-tetOAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
-
TwinStrep-SUMO-Pdp1
Plasmid#198320PurposeFor affinity purification of Pdp1 payloadDepositorInsertTwinStrep-SUMO-Pdp1
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
FGD5
Plasmid#28109PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertFGD5:HPC09X-H08:C212499 (FGD5 Human)
UseTagsHis-tag with TEV siteExpressionBacterialMutationcontains amino acid residues 889-1304.PromoterAvailable sinceMarch 21, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
Reb_140 aka PAS625
Plasmid#71230PurposeReb_1 with RebA(S88P)DepositorInsertReb locus
UseTagsnoneExpressionBacterialMutationRebA(S88P)PromoterAvailable sinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETM30-ARC105
Plasmid#133426PurposeHuman ARC105 coding sequence in vector for in vitro transcription and protein expression, with T7 promoter.DepositorInsertPCQAP (MED15 Human)
UseTagsExpressionBacterialMutationContains amino acids 5-78 fused to C-terminal of …PromoterAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-EGFAB
Plasmid#25770DepositorInsertLDL receptor EGFAB (LDLR Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceDec. 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET30a-SARS-CoV2-NP-Nt(47-173)
Plasmid#165091Purposeprotein expression in E. coliDepositorInsertSevere acute respiratory syndrome coronavirus 2 nucleocapsid protein N-terminal(47-173) (N SARS-CoV2)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 22, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
Dapp1-PH pET11a
Plasmid#71376PurposePhosphoinositide BindingDepositorInsertDapp1-PH (DAPP1 Human)
UseTagsExpressionBacterialMutationPleckstrin homology (PH) domain; aa 148-273PromoterT7Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
BAX KO vector
Plasmid#16575DepositorInsertBAX knockout (BAX Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
pTXB3-Flag PLK1
Plasmid#68271Purposebacterial expression of Plk1DepositorInsertPLK1 (PLK1 Human)
UseTagsFlag and Mxe intein/chitin binding domainExpressionBacterialMutationPromoterT7Available sinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGBdest-GST-PI3Kc1
Plasmid#187992PurposePlasmid for the protein expression of GST-3C-ATG14/Vps34/Vps15/BECN1 in a bacterial and insect expression system. Internal_Reference: SMC1326DepositorUseTagsGSTExpressionBacterial and InsectMutationPromoterAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJB37
Plasmid#202602PurposeArabinose inducible expression vector expressing retron Rcat-Sen2DepositorInsertRcaT-Sen2 toxin
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a(TIGIT)
Plasmid#177902PurposeBacterial expression of his10-tagged human TIGIT Intracellular DomainDepositorInsertTIGIT (aa 163-244 only) (TIGIT Human)
UseTags10xHis-Thrombin cut site-T7 tagExpressionBacterialMutationPromoterT7Available sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
-
His-NKX3.1
Plasmid#42896DepositorInsertNKX3.1 (NKX3-1 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceApril 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+) 6xHis-TEV-IFIT3
Plasmid#53559Purposebacterial expression of IFIT3DepositorInsertIFIT3 (IFIT3 Human)
UseTags6xHIS and TEVExpressionBacterialMutationPromoterT7Available sinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKCyR5
Plasmid#149463PurposeBroad-Host range expression vector, RK2 and pUC origin, CymR(AM) repressor, PCymRC promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertCymR(AM)-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPCymRCAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMCSG53_Nsp15
Plasmid#167265PurposeBacterial expression of Nsp15DepositorInsertNsp15 (residues 1-346) (ORF1ab SARS-CoV-2)
UseTagsHis6-TEV siteExpressionBacterialMutationnonePromoterAvailable sinceMay 5, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEX4T1-TEV-HsFbxo7-129-398/Skp1
Plasmid#202592PurposeBacterial expression of HsFbxo7-129-398 and HsSkp1DepositorUseTagsGST, TEVExpressionBacterialMutationaa 129-398 onlyPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTP443
Plasmid#216361PurposexNup98-Nb1t (+1Cys) Nanobody productionDepositorInsertxNup98-Nb1t (+1Cys)
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLD-CAG-DYSF-CFL
Plasmid#131457PurposeEncodes Dysferlin protein (Therapeutic gene) along with Follistatin protein to cure LGMD2B along with luciferase enzyme for in vivo live imagingDepositorUseTagsExpressionBacterial and MammalianMutationR15C (in DYSF) and Q2579H (in CFL)PromoterAvailable sinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-PA-Rac1-C450A
Plasmid#22025PurposeExpression of Rac1 fused with light-insensitive LOV2 (C450A mutation)DepositorInsertPA-Rac1-C450A (RAC1 Human, Avena sativa (oat))
UseTags6X HisExpressionBacterial, Insect, and Mamm…MutationLOV domain contains light-insensitive mutation C4…PromoterCMV, p10Available sinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pDYU1B/mRuby3–α-tubulin
Plasmid#154302PurposeExpresses mRuby3–α-tubulin from actin15 promoter in Dictyostelium discoideumDepositorInsertalpha-tubuin
UseDictyostelium expressionTagsmRuby3ExpressionBacterialMutationPromoterAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTricHis-PLY
Plasmid#160537PurposeExpression of inserted PLY variantDepositorInsertPLY-WT
UseTagsHis-TagExpressionBacterialMutationPromoterAvailable sinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 NEDD4 WW domain #3
Plasmid#104210PurposeBacterial expression of WW domain #3 from NEDD4DepositorInsertNEDD4 WW domain #3 (NEDD4 Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMD19T-psba1-TetR-PL22-dCas9-SpR
Plasmid#73223PurposeContains dCas9 from S. pyogenes under aTc inducible promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation.DepositorInsertdCas9 from S. pyogenes
UseTagsc-mycExpressionBacterialMutationSilent mutation at bp 1341 A->C to remove an E…PromoterPL22Available sinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
TOPO CFP
Plasmid#68489PurposeTOPO construct expressing CFP for generation of GMAP-compatible geneDepositorInsertCyan Fluorescent Protein
UseGmapTagsExpressionBacterialMutationPromoterPlacAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
-
pET51b-His-TEV-HaloTag7_dead_mt
Plasmid#167267PurposeOverexpression of the dead variant (D106A) of HaloTag7 in E. coliDepositorInsertHaloTag7
UseTagsHis x10ExpressionBacterialMutationD106APromoterT7Available sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-Streptavidin-Dead Aspartate Loop
Plasmid#46368DepositorInsertStreptavidin-Dead Aspartate Loop
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pOxyR_gfp
Plasmid#194154PurposeGreen Fluorescent Reporter of intracellular hydrogen peroxideDepositorInserttranscriptional regulator, OxyR, cognate promoter PoxyS, fluorescent protein sfGFP
UseTagsExpressionBacterialMutationPromoterPoxyR, PoxySAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMTTH+mEGFP-LANA
Plasmid#157801PurposeExpression of mEGFP and LANA peptide fusion proteinDepositorInsertmEGFP-LANA
UseTagsHis6 and MBPExpressionBacterialMutationPromoterpTACAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJMD14
Plasmid#138654PurposephiOZJ integrating Streptomyces expression vector, intact RDFDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterPermE* variant sequence from pDA1652Available sinceApril 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pdUA-lacIN
Plasmid#141079PurposePlasmid vector for testing DNA binding proteins by the I-Block assay.DepositorTypeEmpty backboneUseVector for the i-block assay designed to study dn…TagsExpressionBacterialMutationPromoterEscherichia coli lacIN promoterAvailable sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only