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32,671 results

Showing: 8501 - 8550 of 32671 results

  1. pAGD289

    Plasmid
    #228113
    Purpose
    pET-21(+) deriviative with retron Kva1 containing a donor to edit E. coli rpoB gene cloned downstream of a T7 promoter
    Depositor
    Seth Shipman
    Article
    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103
    Insert
    Kva1 retron, ncRNA with 90 bp rpoB donor
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    228113-plasmid-map-sequence-id-455374

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  10. pETM33_Nsp3d_Ub2_PLPro

    Plasmid
    #156472
    Purpose
    Bacterial expression of Sars-CoV2 Nsp3d_Ub2_PLPro protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Article
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    Nsp3d_Ub2_PLPro (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available Since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    156472-plasmid-map-sequence-id-307744

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  11. pTriEx-mVenus-PA-Rac1-T17N

    Plasmid
    #22017
    Purpose
    Expression of photoactivatable Rac1 (dominant-negative mutant)
    Depositor
    Klaus Hahn
    Article
    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.
    Insert
    PA-Rac1-T17N (RAC1 Avena sativa (oat), Human)
    Tags
    6X His and mVenus
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    Rac1 starts at I4 and contains mutation T17N.
    Promoter
    CMV, p10
    Available Since
    Sept. 29, 2009
    Availability
    Academic Institutions and Nonprofits only
    22017-plasmid-map-sequence-id-138512

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  18. CTPSA

    Plasmid
    #42343
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CTPSA (CTPS1 Human)
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  25. pcDNA6.MCV.cLT206.V5(CM2B4)

    Plasmid
    #28189
    Depositor
    Patrick Moore
    Insert
    MCPyV Large T antigen (206 wild-type strain)
    Tags
    V5
    Expression
    Bacterial and Mammalian
    Available Since
    Sept. 28, 2011
    Availability
    Academic Institutions and Nonprofits only
    28189-plasmid-map-sequence-id-422374

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  26. pETM-30-GST-PfAgo_F

    Plasmid
    #146265
    Purpose
    Bacterial Expression of PfAgo
    Depositor
    Elisa Izaurralde
    Insert
    PfAgo
    Expression
    Bacterial
    Mutation
    one silent T1704C and one non silent mutation A90…
    Available Since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    146265-plasmid-map-sequence-id-456974

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  29. pCR8/GW/TOPO BMI

    Plasmid
    #69796
    Purpose
    Gateway entry vector for BMI
    Depositor
    Gerard Cagney
    Insert
    BMI (BMI1 Human)
    Expression
    Bacterial
    Available Since
    Oct. 23, 2015
    Availability
    Academic Institutions and Nonprofits only

  39. pExp-IF2

    Plasmid
    #112567
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and IF2 (domain I of translation initiation factor IF-2 from E. coli) fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8xHis-IF2 and Strep tag
    Expression
    Bacterial
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112567-plasmid-map-sequence-id-217215

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  40. pHflu4

    Plasmid
    #203882
    Purpose
    pHI1 with insertion of monomeric red fluorescent protein (mRFP)
    Depositor
    Bogumil Karas
    Insert
    monomeric red fluorescent protein
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available Since
    Dec. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    203882-plasmid-map-sequence-id-403835

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  41. pQ-Elo-HB-C-A(1-630)

    Plasmid
    #171086
    Purpose
    Co-expresses of ΔC-ELOA containing Elongin complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding all 3Elongin subunits, with a 6-His tag on ELOB
    Depositor
    Joan Conaway
    Article
    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11
    Inserts
    Elongin B (ELOB Human)
    Elongin C (ELOC Human)
    Elongin A (ELOA Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Deleted N-term 26aa and C-term 142aa for mutant e…
    Promoter
    T5 promoter
    Available Since
    July 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    171086-plasmid-map-sequence-id-335555

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  43. FnR9-10

    Plasmid
    #182567
    Purpose
    Human fibronectin fragment, type-III repeats 9-10, R1379A mutant
    Depositor
    Timothy Springer
    Article
    Su et al J Biol Chem. 2022 Sep;298(9):102323. doi
    Insert
    Human fibronectin fragment, type-III repeats 9-10
    Tags
    His and TEV cleavage site after His tag
    Expression
    Bacterial
    Mutation
    R1379A
    Available Since
    Oct. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182567-plasmid-map-sequence-id-357181

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  44. Avr2-GFP pZK538

    Plasmid
    #118011
    Purpose
    expresses Avr2-GFP (without signal peptide) and mCherry-HDEL. Used for visualization of cell-to-cell movement of Avr2. Avr2 can be replaced with gene of interest via HiFi DNA assembly cloning (NEB) .
    Depositor
    FLW Takken
    Article
    et al Bio-protocol 8(24): e3114.
    Inserts
    dspAvr2
    mCherry
    Tags
    EGFP and HDEL
    Expression
    Bacterial and Plant
    Promoter
    35S
    Available Since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    118011-plasmid-map-sequence-id-307579

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  45. pMCSG53_Nsp9

    Plasmid
    #167264
    Purpose
    Bacterial expression of Nsp9
    Depositor
    Alexei Savchenko
    Insert
    Nsp9 (residues 1-113) (ORF1ab SARS-CoV-2)
    Tags
    His6-TEV site
    Expression
    Bacterial
    Mutation
    none
    Available Since
    April 27, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    167264-plasmid-map-sequence-id-331338

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  46. p404GAL1

    Plasmid
    #17417
    Depositor
    Nicolas Buchler, Fred Cross
    Insert
    GAL1 promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
    Expression
    Bacterial and Yeast
    Mutation
    GAL1 promoter is between SacI and XbaI restrictio…
    Available Since
    March 12, 2008
    Availability
    Academic Institutions and Nonprofits only
    17417-plasmid-map-sequence-id-8592

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  50. pET22b Ag43 160N 6H sfGFP-R5 AmpR

    Plasmid
    #225139
    Purpose
    Recombinant Ag43 protein fused to silaffin-R5 and sfGFP. pelB as the periplasmic signal. His-tag for purification. TEV enzyme recognition site between Ag43 alpha subunit and the fluorescent protein.
    Depositor
    Urartu Seker
    Article
    Kirpat Konak et al Mater Today Bio. 2022 Oct 10;17:100461.
    Insert
    Ag43 protein fused to silaffin-R5 and sfGFP
    Tags
    His tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    225139-plasmid-map-sequence-id-460505

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Showing: 8501 - 8550 of 32671 results