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Plasmid#90488PurposePlasmid with ptb promoter for gene expression in Clostridium acetobutylicum ATCC 824 and Clostridium beijerinckii NCIMB8052DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only
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pCas9-beta-mmr
Plasmid#169241PurposeCas9, beta, and mutL mutant expression plasmid for introducing chromosomal point mutationsDepositorInsertscas9
beta
mutL-E36K
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationChanged glutamic acid 36 to lysinePromoterAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-RUNX2(WT)-IDR-mCherry
Plasmid#145286PurposeBacterial Expression of 6xHis RUNX2(WT) IDR mCherry Fusion ProteinDepositorInsertRUNX2(WT)-IDR (RUNX2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET29b-EPN-01*
Plasmid#80423PurposeFor expression of EPN-01* in E. coliDepositorInsertEPN-01*
UseTagsMycExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX6p2rbs-GST-Ring1b(1-159)-Bmi1(1-109)
Plasmid#63139PurposeExpression of murine Ring 1b (1-159 aa) and Bmi1 (1-109 aa)DepositorUseTagsGSTExpressionBacterialMutationResidues 1-109 and Residues 1-159PromotertacAvailable sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
human p53-Δ(325-355)
Plasmid#24871DepositorInsertp53 (TP53 Human)
UseTagsHisExpressionBacterialMutationDeletion of aa 325-355PromoterAvailable sinceAug. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAB088i
Plasmid#79216PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI(RR69)-TnCAD-F3
UseTagsExpressionBacterialMutationPromoterPlacZ-opt (OR1); Ppro1Available sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET12a-AL-09 FL
Plasmid#47081DepositorInsertAL-09 (IGKV3-20 Human)
UseTagsompTExpressionBacterialMutationPromoterT7Available sinceAug. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
KTK_354
Plasmid#180525PurposeKTK compatible Intergration/suicide plasmid. Contains a LacZ dropout cassette flanked by regions homolgous to the arsenic resistance cassete.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
p_SNIPR_target_mut_36
Plasmid#139466PurposeT7 RNAP-driven expression of a non-cognate SNIPR trigger RNA with a mutation at position 36DepositorInsertTarget_mut36
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 NEDD4L WW domain #2
Plasmid#104205PurposeBacterial expression of WW domain #2 from NEDD4LDepositorInsertNEDD4L WW domain #2 (NEDD4L Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNIC-ZB-SPRTNwt-FL
Plasmid#110217PurposeExpression in E. coli of full-length wild-type SPRTN protein, with His and ZB tags at N-terminus.DepositorInsertSPRTN (SPRTN Human)
UseTagsExpressionBacterialMutationP296L (see depositor comments below)PromoterAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pScaf-10080.1
Plasmid#111410PurposepScaf phagemid with insert for generating DNA origami scaffold 10080 bases in lengthDepositorInsertpScaf-ACKN
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDSG288
Plasmid#115607Purposesurface-displayed Ag3, used in composability experiments; low copyDepositorInsertregulated adhesin construct
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6.MCV.LT206.V5(CM2B4)
Plasmid#28190DepositorInsertMCPyV genomic T antigen locus (206 wild-type strain)
UseTagsV5ExpressionBacterial and MammalianMutationPromoterAvailable sinceMay 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pH
Plasmid#64178Purposeharbours marker gene HIS3DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
GST-AR-1-660
Plasmid#104196PurposeExpresses human AR-1-660 from bacterial expression vector pGEX-5X-1 as GST fusion proteinDepositorInserthuman androgen receptor aa 1-660 (AR Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-NtLAtC60αβC20
Plasmid#160873PurposeExpression of N. tabacum Rubisco large subunit and A. thaliana Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coliDepositorInsertrbcL, cpn60α, cpn60β, cpn20
UseTagsExpressionBacterialMutationPromoterrbcL: pBAD, chaperones: T7Available sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLN
Plasmid#40028DepositorInsertsloxP
Neo
UseTagsExpressionBacterial and MammalianMutationPromoterNone and SV40Available sinceDec. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHS1929
Plasmid#205968PurposeJAGBLH010000220 Cas8-HNH crRNA expression in pCOLADuet-1DepositorInsertCas8-HNH crRNA
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPPi-260n
Plasmid#13552DepositorInsertFLIPPi WT
UseTagsECFP and EYFPExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pTriEX-Antenna-GDI Rac1
Plasmid#83359PurposeExpression of Antenna-Rac1DepositorInsertRac1 (RAC1 Human)
UseTagsFRET Antenna (mCerulean-cpVenus) and HisExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceMarch 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBabe-PI(WT)-Src(YF)-mCherry
Plasmid#87357PurposePI(WT): Photo-inhibitable/blue light sensitive; YF: Y535F const. active mutationDepositorInsertsUseTagsmCherryExpressionBacterial and MammalianMutationY535FPromoterCMVAvailable sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-GP-2-ATRX ADD
Plasmid#59698PurposeContains histone modification interacting domain (ATRX ADD)DepositorInsertATRX ADD (ATRX Human)
UseTagsGSTExpressionBacterialMutationPromotertac promoterAvailable sinceOct. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
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HS-mKate2
Plasmid#105945Purposeheat shock inducible transgenesis, cytosolic mKate2DepositorInsertmKate2
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pACYC.Taq.1Amb
Plasmid#102794PurposeExpresses Taq DNA polymerases with a premature stop codonDepositorInsertTaq DNA polymerase
UseTagsExpressionBacterialMutationW168Stop; *(see below)PromoterAvailable sinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
DLG4_PDZ_3
Plasmid#103953PurposeProtein expression and purification of PSD95 PDZ3 domainDepositorInsertDLG4 (DLG4 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEE077
Plasmid#176834PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert716
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPlacPbuCas13b-gRNA-T-MS2
Plasmid#184839PurposeExpression of a single-spacer CRISPR array with spacer #1 targeting the MS2 phage genome and expression of PbuCas13b in bacteria.DepositorInsertsingle-spacer CRISPR array with spacer #1 targeting the MS2 phage genome, PbuCas13b nuclease
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119 and lac promoterAvailable sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-pro-siRNA-V2-TP53
Plasmid#111089PurposepET-pro-siRNA-V2 based plasmid for production of recombinant siRNAs (pro-siRNAs) against human TP53 gene.DepositorInsertTP53 (TP53 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ALDOA_Glycolytic
Plasmid#109854PurposeProtein expression and purification of ALDOA_GlycolyticDepositorInsertALDOA_Glycolytic (ALDOA Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
HM3C NaVAe1_p pET24b
Plasmid#127854PurposeBacterial expression vector for NaVAe1 bacterial sodium channel residues 143-288. It expresses HIStag-MBP-3C-channel protein.DepositorInsertNaVAe1p
UseTags3C Cleavage site, His, and MBPExpressionBacterialMutationPromoterAvailable sinceMay 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T_PDZ2
Plasmid#59735Purposeexpresses PDZ2 domain of human ZO-1 in bacteriaDepositorInsertzonula occludens-1 (TJP1 Human)
UseTagsGST and thrombin clvgExpressionBacterialMutationPromoterAvailable sinceSept. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET16b-Mms2
Plasmid#18893DepositorInsertYeast ubiquitin conjugating enzyme Mms2 (MMS2 Budding Yeast)
UseTagsHIS10ExpressionBacterialMutationPromoterAvailable sinceSept. 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBSMrnaStrp
Plasmid#67508PurposeExpress the tRNAMet scaffold with the streptavidin aptamer tagDepositorInsertmodified tRNAMet with streptavidin aptamer
UseTagsExpressionBacterialMutationPromoterlppAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMM555
Plasmid#68880PurposeNanoLuc expressed constiutively from PcfxA, pNBU2 backbone, AmpRDepositorInsertNanoLuc
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS chick islet1 [TJ#44]
Plasmid#16273DepositorInsertislet1 (ISL1 Chicken)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 4, 2009AvailabilityAcademic Institutions and Nonprofits only -
Clover2-pBAD
Plasmid#54576PurposeLocalization: Protein Expression Vector , Excitation: 505, Emission: 515DepositorTypeEmpty backboneUseTags6xHis and Clover2ExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSL0374 (His-MBP-Cas8_Cas7_Cas6)
Plasmid#130639PurposeExpresses V. cholerae CAST Cas8, Cas7, and Cas6 from a T7 promoter, with N-terminal His10-MBP-TEVsite tag on Cas8 for purification.DepositorInsertVchCas8, VchCas7, VchCas6
UseCRISPR; TransposonTagsHis10-MBP-TEVsite on Cas8ExpressionBacterialMutationPromoterT7Available sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0057 BzoCas13 (B01) His6-TwinStrep-SUMO-BsaI
Plasmid#115207PurposeExpresses BzoCas13b from a SUMO-tagged bacterial expression constructDepositorInsertBzoCas13
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNIC-TolB-NHSTIIT-GG
Plasmid#215792PurposeCloning of target gene with N terminal TolB SRP pathway signal peptide N terminal His6 Twin Strep tag and TEV cleavageDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEM10686
Plasmid#213995PurposePrpsM driven expression of mScarlet-IDepositorInsertmScarlet-I
UseTagsExpressionBacterialMutationPromoterPrpsMAvailable sinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET11a-STEPtag
Plasmid#159373PurposeExpresses STEPtag in E. coliDepositorInsertSTEPtag
UseTags6x His tagExpressionBacterialMutationPromoterT7Available sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pUC18T-mini-Tn7T-Zeo-ecfp
Plasmid#65038PurposeZeoR mini-Tn7 vector, mobilizable, for CFP tagging bacteriaDepositorInsertecfp
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOPINEneo-3C-GFP
Plasmid#53534PurposepTirex based GFP fusion vector for expression in mammalian, E. coli and insect cells using the baculovirus systemDepositorInsertGFP
UseTagsGFP-His8ExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
Z-VCP-10
Plasmid#166559PurposescFv of a human scaffold targeting Transitional endoplasmic reticulum ATPase. Antigen coverage aa 1-806 of 806, full-lengthDepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialMutationPromoterLacZAvailable sinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEM09
Plasmid#135479PurposeSpizellomyces Hsp70 promoter driving expression of Hygromycin resistance fused to GFPDepositorInsertSpunHsp70pr
UseTagsGFP and hphExpressionBacterial and YeastMutationPromoterSpun Hsp70prAvailable sinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
MetNI (Lm)
Plasmid#78316PurposeMethionine ABC TransporterDepositorInsertsMetI
MetN
UseTags10x Histidine Tag and 6x Histidine TagExpressionBacterialMutationPromoterT7 PromoterAvailable sinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only