31,059 results
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Plasmid#177858PurposeBacterial Expression of TPR1DepositorAvailable SinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only
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pOPINNUSA
Plasmid#41119DepositorTypeEmpty backboneExpressionBacterial, Insect, and Mamm…Available SinceMarch 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTCF10 HLA-B*08:01
Plasmid#180447PurposeE. coli expression of soluble MHC proteinDepositorInsertHLA-B*08:01
TagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…Available SinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBR5pemIKpKan
Plasmid#129271PurposeBroad host-range plasmid with toxin-antitoxin system for maintenance in the absence of antibiotic selection, and constitutive promoterDepositorTypeEmpty backboneExpressionBacterialAvailable SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTns(Ptr)
Plasmid#175576PurposeExpression of CRISPR-associated transposasesDepositorInsertPtrTnsABC
ExpressionBacterialMutationNOAvailable SinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pL1M-pMtRC-MtLAP1-Adh-R1
Plasmid#170790PurposeA Root Tip-Specific Expressing Anthocyanin Marker for Direct Identification of Transgenic Tissues by the Naked Eye in Symbiotic StudiesDepositorInsertMtLAP1 (LOC11412013 Medicago truncatula)
ExpressionBacterial and PlantPromoterMedtr4g059670(MtRC)Available SinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-SPA2G231V
Plasmid#42079DepositorInsertSurfactant Protein -A2 (SFTPA2 Human)
UseLentiviral; PlentiTagsV5ExpressionBacterial and MammalianMutationChanged Glycine 231 to ValinePromoterCMVAvailable SinceMarch 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-143
Plasmid#118527PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertsYFP2
UseMinitn5 delivery vectorExpressionBacterialAvailable SinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pThermoCas9i_ctrl
Plasmid#100982PurposeExpresses the catalytically inactive version of ThermoCas9 (Thermo(d)Cas9) and its sgRNA moduleDepositorInsertsCatalytically inactive variant of the Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
ThermoCas9 single guide RNA expressing module
TagsNoneExpressionBacterialMutationchanged aspartate 8 to alanine and histidine 582 …PromoterB. coagulans DSM 1 pta promoter and B. smithii xy…Available SinceNov. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-28-Strep-OPA1(253-960) (SB263)
Plasmid#227612PurposeInducible expression of Strep-tagged Opa1(253-960) in E.coliDepositorInsertOPA1 (OPA1 Human)
TagsTwin-StrepTagExpressionBacterialMutationcontains aa 253-960 onlyPromoterT7Available SinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
WDV-Echistatin
Plasmid#226985PurposeExpresses the HUH endonuclease WDV fused at the C termini with Echistatin to target RGD binding integrins with DNA tension sensorsDepositorAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQE81XN-msYFP
Plasmid#218960PurposeExpresses Hisx6-Tagged msYFP in bacteriaDepositorInsertmsYFP
Tags6X-HisExpressionBacterialPromoterT5Available SinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACYC-T7-Nla-repeat-BbsI
Plasmid#180215PurposeFor cloning Neisseria lactamica Type I-C crRNA for protein purification in E.ColiDepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterT7Available SinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIA1385
Plasmid#166862PurposeCodon optimized SARS-CoV-2 Nsp12 gene was cloned into a modified pRSFDuet-1 vector (Addgene #159107) for expression in E. coli.DepositorAvailable SinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
TDP-43_CTD_A321V
Plasmid#98671PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43 with A321VDepositorAvailable SinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pdCas9+sgRNA expression vector precursor
Plasmid#171798PurposePICASSO: Library expression vector precursor for paired dCas9+sgRNA expressionDepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterpLtetOAvailable SinceAug. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-tdMCP-12xGCN4
Plasmid#205158Purposeexpress tdMCP-12xGCN4DepositorInserttdMCP-12xGCN4
ExpressionBacterial and MammalianPromoterCMVAvailable SinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD-eLACCO2.1
Plasmid#208017PurposeBiosensor for extracellular L-lactateDepositorInserteLACCO2.1
ExpressionBacterialAvailable SinceOct. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_HNRNPA1-Halo
Plasmid#175307PurposeProduction of HaloTag fusion proteinDepositorAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETNKI-strepII-3C-LIC-Amp
Plasmid#108707PurposeExpression of a StrepII-tagged target protein with 3C protease cleavage site.DepositorTypeEmpty backboneTagsStrepII-3C protease cleavage siteExpressionBacterialPromoterT7Available SinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pGEX3X-RanBP2dFG
Plasmid#53120PurposeE.coli expression of GST tagged human RanBP2 fragment with SUMO ligase activityDepositorInsertRanBP2 fragment (RANBP2 Human)
TagsGSTExpressionBacterialMutationfragment (amino acids 2553-2838)PromotertacAvailable SinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pOPINN-GFP
Plasmid#53541PurposepTirex based GFP fusion vector for expression in mammalian, E. coli and insect cells using the baculovirus systemDepositorInsertGFP
TagsN-His-GFPExpressionBacterial, Insect, and Mamm…Available SinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKL004
Plasmid#98920PurposeConstitutively expresses the calcium sensor GCaMP6f tethered to mRuby3 under the Sigma 70 promoter in E. coli.DepositorInsertGCaMP6f tethered to mRuby
TagsHis and mRuby3ExpressionBacterialPromoterBBa_J23118 - Strong ConstitutiveAvailable SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-NT/pLux eGFP
Plasmid#193624PurposeInducible expression of eGFP by the transcription factor complex LuxR-3OC6 homoserine lactone (HSL).DepositorInserteGFP
Tags6xHisExpressionBacterialPromoterpLuxAvailable SinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-NT/pT7 LuxR
Plasmid#193625PurposeConstitutive T7 RNAP-mediated expression of LuxR transcription factor for Lux quorum sensing system. LuxR sequence is codon optimized for E. coli.DepositorInsertLuxR
Tags6xHisExpressionBacterialPromoterT7 RNAP promoterAvailable SinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBK086
Plasmid#183145PurposepET-6xHis-MBP-TEV-MapTIR-APAZ/MapAgo - Heterolgous MapSPARTA expressionDepositorInsertMapSPARTA operon (6xHis-MBP-MapTIR-APAZ and MapAgo)
Tags6xHis and MBPExpressionBacterialPromoterT7Available SinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBbE5a-RFP
Plasmid#35277DepositorInsertRFP
UseSynthetic BiologyExpressionBacterialPromoterlacUV5Available SinceFeb. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLC133
Plasmid#198639PurposeExpression plasmid for dCas9 in E. coli used in Chip-seq experiments. dCas9 is tagged with a C-terminal 3x FLAG tag. A guide RNA can be cloned using BsaI restriction sites.DepositorInsertdCas9
Tags3x-FLAGExpressionBacterialPromoterpTetAvailable SinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Mucin MUC1-5TR-pET15b
Plasmid#174694PurposePlasmid expressing five copies of the MUC1 tandem repeat (MUC1-5TR)DepositorInsertMUC1-TR
Tagshexahistidine (HIS) tagExpressionBacterialMutationnonePromoterT7 PromoterAvailable SinceNov. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;cldn5bP1Egfp
Plasmid#90158Purposecontains cldn5b specific gene promoter driving expression of eGFPDepositorAvailable SinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSHS212
Plasmid#87346PurposeCysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmidDepositorInsertCas9
TagsHis-MBPExpressionBacterialMutationC80S/C574SPromoterT7Available SinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMUT2-VtrA-mCherry
Plasmid#192862PurposepMUT2 based vector for sensing human secondary bile acids in E. coli Nissle 1917DepositorInsertVtrA-CadC fusion protein and cognate promoter inducible by secondary bile acids
ExpressionBacterialAvailable SinceApril 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pMJ824
Plasmid#39314DepositorInsertCas9
UseCRISPRTagsGST and His6ExpressionBacterialPromoterT7Available SinceSept. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pKB231
Plasmid#170018PurposeEncodes LacI-controlled expression of surface-displayed cecropin P1 and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
Cecropin P1
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialPromoterPtac and PvirKAvailable SinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
SUV420H2
Plasmid#32864PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceJan. 6, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
enz.083-NMT1
Plasmid#154047PurposeExpresses NMT1 in E. coliDepositorAvailable SinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-Sac-Abeta(MC1-40)
Plasmid#127152PurposeExpresses N-terminal cysteine Abeta(1-40)DepositorAvailable SinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAT588
Plasmid#68907PurposeNanoLuc expressed constiutively from PBT1311 and GH023 RBS, pNBU2 backbone, AmpRDepositorInsertNanoLuc
ExpressionBacterialAvailable SinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only