31,059 results
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Plasmid#64168Purposeharbours marker genes HIS3, LEU2 and MET17DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only
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pXD70LacZ2-Pdadh-LacZ
Plasmid#191618PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), Pdadh dopamine dehydroxylase promoter-lacZDepositorInsertlacZ
ExpressionBacterialAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
CPSF5A
Plasmid#42353PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRW004-SM-destination-proUBQ10-Cas9
Plasmid#104438PurposeDestination vector expressing plant-codon-optimized Cas9 under UBQ10 promoter, with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free plants;DepositorTypeEmpty backboneUseCRISPRExpressionBacterial and PlantPromoterUBQ10Available SinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEE050
Plasmid#176807PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert601
Tags6xHisExpressionBacterialPromoterT7Available SinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
H14_MBP_SUMO-Cys-linker-BAF
Plasmid#101777PurposeExpresses His-MBP-tagged BAF in E.coli (SUMO-cleavable)DepositorAvailable SinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_NR1D1_Hormone-recep
Plasmid#109960PurposeProtein expression and purification of NR1D1_Hormone-recepDepositorInsertNR1D1_Hormone-recep
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
FCM101
Plasmid#194298PurposeE. coli expression vector for monobody adaptor, FCM101, that bind to the Fc region of mouse IgG1 subclass. It contains a single Cys residue at the C-terminus for chemical conjugation, N-terminal His6DepositorInsertFCM101
TagsHis tag, FLAG tagExpressionBacterialPromoterT7Available SinceMarch 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCJH002_10xHis-MBP-TEVcs-Cas12c(4)_Amp
Plasmid#183069PurposeBacterial protein expression plasmid of wild-type Cas12c_4. This is a R965H version of the Cas12c in Harrington et al., 2020.DepositorInsertCas12c_4
UseCRISPRTagsHis10 and MBPExpressionBacterialMutationWild-typeAvailable SinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTXG
Plasmid#52280PurposeBacterial expression of TARGET proteins C-terminally fused to GyrA intein and GSTDepositorTypeEmpty backboneTagsGyrA-intein-GSTExpressionBacterialPromoterT7Available SinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-41
Plasmid#172757PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-41; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-41
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-BoNT/E
Plasmid#168020PurposeExpresses BoNT/E-LC(1-411)-6xHis in bacterial cellsDepositorInsertBoNT/E-LC(1-411)-6xHis
TagsHisx6ExpressionBacterialAvailable SinceNov. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBT3011
Plasmid#122539PurposeExpression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a lysosomal localized gene (cup-5) under the intestinal specific promoter nhx-2DepositorAvailable SinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
hSDH5_C83D
Plasmid#113048PurposeExpression of mutant hSDH5DepositorAvailable SinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGV3340
Plasmid#170278PurposeExpression of mutant HIV-2 RT p51 reverse transcriptaseDepositorInsertHIV-2 RT p51 (mutant)
TagsHexahistidineExpressionBacterialPromoterT7-lacAvailable SinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPglu-600uDelta13
Plasmid#13559DepositorInsertFLIPglu F16A Delta13
TagsECFP and EYFPExpressionBacterialMutationF16A in binding proteinAvailable SinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
mCD8-GFP in pBS
Plasmid#17745DepositorAvailable SinceMay 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
pR1G108CP20X
Plasmid#85086PurposeExpresses PCNA1 variant (G108C) fused to putidaredoxin reductase and putidaredoxin in E. coliDepositorInsertThe G108C variant of PCNA1 fused to putidaredoxin reductase and putidaredoxin
TagsHis6 tagExpressionBacterialMutationG108C in PCNA1PromoterT7 promoterAvailable SinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJUMP46-2A(sfGFP)
Plasmid#126990PurposeLevel 2 vector. sfGFP reporter. Origin p15A (medium copy number)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialMutationNoneAvailable SinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX Stat5B-SH2
Plasmid#46516DepositorInsertStat5B (STAT5B Human)
TagsAviTag, GST, and PreScissionExpressionBacterialMutationcontains amino acids 593-692PromoterTacAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX NBP1 (P#415)
Plasmid#14658DepositorInsertATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H (ATP6V1H Human)
ExpressionBacterialAvailable SinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
pKW08-Lx-Int
Plasmid#25015DepositorInsertpKW08-Lx-Int
ExpressionBacterialAvailable SinceAug. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
HOXB7 (human) FLAG pSP65
Plasmid#8537DepositorInsertHOXB7 (HOXB7 Human)
UseIn vitro transcription/translationTagsFLAGExpressionBacterialMutationstarts at AA#1 preceeded by FLAG and Ser-Arg-Ile…Available SinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET28a-pro-sumo2
Plasmid#25102DepositorAvailable SinceJune 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCPP5108
Plasmid#128726PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopAA1-2
ExpressionBacterialAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCPP5107
Plasmid#128725PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopAA1-1
ExpressionBacterialAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSUMO His6 SUMO PYL1 (36-211)
Plasmid#177843PurposeBacterial Expression of PYL1DepositorAvailable SinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
MAT1A
Plasmid#38852PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX-GST-AGO4
Plasmid#24319DepositorInsertAGO4 (AGO4 Human)
ExpressionBacterialAvailable SinceMay 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pPro24
Plasmid#17805DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceApril 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
PIM2
Plasmid#38867PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pnxABE-flgn
Plasmid#187932PurposeA-to-G base editorDepositorInsertecTadA(8e)-nxCas9 (D10A)
UseCRISPRExpressionBacterialAvailable SinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWT009d
Plasmid#107888PurposeR6 plasmid. Contains PBAD-EGFP and is part of part of the CAMERA system for rewritable multi-event analog recording in bacteriaDepositorInsertPBAD-EGFP
ExpressionBacterialAvailable SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4136458
Plasmid#149531PurposeBacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
Tags6xHIS;Linker_GGSG;SUMO tag;Linker_GGSG;Cleavable …ExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceMay 13, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET3a-Ub-D77
Plasmid#18896DepositorInsertUbiquitin-D77 (UBC Human)
ExpressionBacterialMutationAspartate residue is added to the C-terminus of t…Available SinceSept. 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
6BYB (L3MBTL1)
Plasmid#119957PurposeBacterial expression for structure determinationDepositorAvailable SinceJan. 8, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
ELF3_pet28a
Plasmid#131658PurposeBacterial expression of ETS gene ELF3DepositorAvailable SinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
E2F-1 wt-pGex2TK
Plasmid#21668DepositorAvailable SinceSept. 4, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBTK1005 (pSL4)
Plasmid#191000PurposeExpresses red chromoprotein on a broad host range RSF1010 origin plasmidDepositorInsertred chromoprotein
UseSynthetic BiologyExpressionBacterialPromoterCP25Available SinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pET30b_T7Sav
Plasmid#138588PurposeT7-tagged streptavidin with native C-terminus controlled by PT7 promoter; KanR, pBR322 oriDepositorInsertT7-tagged streptavidin variant with native C-terminus
UseSynthetic BiologyTagsT7-tagExpressionBacterialPromoterPT7Available SinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX_hDDX5_FL_D248N
Plasmid#88872Purposeinducible expression of GST-DDX5 D248N in bacteriaDepositorAvailable SinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSaPIPLC His
Plasmid#173810PurposeExpression and purification of mature, His-tagged Staphylococcus aureus (strain FPR3757) phosphaatidyl inositol specific phospholipase C (PI-PLC) from E. coli BL21 CodonPlus (DE3) RIL cellsDepositorInsertStaphylococcus aureus plc
Tags6XHisExpressionBacterialMutationnonePromoterT7Available SinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
PET-3a-chpBK
Plasmid#155201PurposeTo express recombinant ChpBK proteinDepositorInsertschpS-chpBK-6×His-Tag
GyrA-CBD-10xHis-Tag
Tags10xHis and 6xHisExpressionBacterialAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTn7xKS
Plasmid#117393PurposeTn7 tagging vector with a LacI-controlled kill switch counterselection systemDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX5X.1 PINK1 KD
Plasmid#13322DepositorInsertPINK1 kinase domain (PINK1 Human)
TagsGSTExpressionBacterialMutationAmino acids 112-496; Kinase dead: K219D/D362A/D3…Available SinceNov. 9, 2006AvailabilityAcademic Institutions and Nonprofits only -
RNF168
Plasmid#25298PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 16, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJJDuet30
Plasmid#11962DepositorInsertSsp DnaE-N
TagsGB1ExpressionBacterialAvailable SinceSept. 21, 2007AvailabilityAcademic Institutions and Nonprofits only -
pFREE2-SpCas9-sgRNA
Plasmid#179582PurposeExpresses spCas9 and gRNA that targets ColE1 plasmids for curingDepositorInsertCas9
ExpressionBacterialAvailable SinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPBO.ACT003
Plasmid#182713PurposeaTc inducible dCasRx-IF3DepositorInsertdCasRx
UseCRISPRTags3x(GGGS)-Escherichia Coli Initiation Factor 3ExpressionBacterialMutationCatalytically deactivated R295A, H300A, R849A, H8…PromoterpTetAvailable SinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only