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31,059 results

Showing: 6921 - 6960 of 31059 results

  2. pETM-30-GST-PfAgo_F

    Plasmid
    #146265
    Purpose
    Bacterial Expression of PfAgo
    Depositor
    Elisa Izaurralde
    Insert
    PfAgo
    Expression
    Bacterial
    Mutation
    one silent T1704C and one non silent mutation A90…
    Available Since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    146265-plasmid-map-sequence-id-456974

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  4. pKEK3228

    Plasmid
    #234090
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Article
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    aeBlue
    Expression
    Bacterial
    Promoter
    pJ23100-BsaI
    Available Since
    April 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    234090-plasmid-map-sequence-id-467993

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  5. pAIP4_2EYI

    Plasmid
    #234173
    Purpose
    Heterologous protein expression of alpha-actinin-1 in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    2EYI (ACTN1 Human)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234173-plasmid-map-sequence-id-464138

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  6. SEC14L3

    Plasmid
    #73240
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SEC14L3 (SEC14L3 Human)
    Tags
    His-6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 25, 2016
    Availability
    Academic Institutions and Nonprofits only

  7. SEC14L4

    Plasmid
    #73233
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SEC14L4 (SEC14L4 Human)
    Tags
    His-6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    73233-plasmid-map-sequence-id-137211

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  9. pHtrA-TEV-His12

    Plasmid
    #137036
    Purpose
    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi HtrA with C-terminal TEV-protease-cleavable His12
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66500.2 (BB_0104 Borrelia burgdorferi B31)
    Tags
    His12 (TEV protease cleavable)
    Expression
    Bacterial
    Mutation
    wild type, full-length protein, contains signal p…
    Promoter
    T7
    Available Since
    March 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    137036-plasmid-map-sequence-id-275699

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  10. pBS-AMVpl-β1WT-nAchR

    Plasmid
    #97080
    Purpose
    To determine if structural domains are important for nicotinic acetylcholine receptor (nAChr) channel function via dose-response curves from voltage-clamped oocytes to estimate EC50's and Hill Co
    Depositor
    Henry Lester
    Article
    Labarca et al Nature. 1995 Aug 10. 376(6540):514-6.
    Insert
    Beta1 nicotinic acetylcholine receptor (Chrnb1 Mouse)
    Expression
    Bacterial
    Mutation
    WT
    Promoter
    T7
    Available Since
    July 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    97080-plasmid-map-sequence-id-189664

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  12. pH6-TEV-mCherry

    Plasmid
    #132430
    Purpose
    Expresses His6-tagged mCherry in E. coli using T7 promoter
    Depositor
    Mark Arbing
    Insert
    mCherry
    Tags
    N-terminal His6 tag with TEV protease site
    Expression
    Bacterial
    Mutation
    Codon optimized
    Promoter
    T7
    Available Since
    Oct. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    132430-plasmid-map-sequence-id-258769

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  13. pBAD-bHs

    Plasmid
    #134259
    Purpose
    For L-arabinose inducible bacterial expression of S. typhimurium codon-optimized hyaluronidase (Streptomyces koganeinsis UniProt A0A0U2E2) with N-terminal 6x His tag
    Depositor
    Edwin Manuel
    Article
    Ebelt et al Mol Cancer Ther. 2019 Nov 6. pii: 1535-7
    Insert
    Hyaluronidase from Streptomyces koganeinsis codon-optimized for Salmonella typhimurium
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    codon optimized for Salmonella typhimurium
    Promoter
    AraC
    Available Since
    Nov. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    134259-plasmid-map-sequence-id-264461

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  19. RALGDS

    Plasmid
    #25331
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    RALGDS:N793-F914 (RALGDS Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids N793-F914
    Available Since
    Sept. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  20. R26-CAG-loxP-FNF-bpA-3pA-loxP-tdTomato

    Plasmid
    #180152
    Purpose
    tdTomato cre reporter with Rosa26 recombineering homology arms
    Depositor
    Mario Capecchi
    Insert
    tdTomato
    Use
    Cre/Lox and Mouse Targeting
    Expression
    Bacterial and Mammalian
    Available Since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180152-plasmid-map-sequence-id-355317

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  23. pAGD289

    Plasmid
    #228113
    Purpose
    pET-21(+) deriviative with retron Kva1 containing a donor to edit E. coli rpoB gene cloned downstream of a T7 promoter
    Depositor
    Seth Shipman
    Article
    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103
    Insert
    Kva1 retron, ncRNA with 90 bp rpoB donor
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    228113-plasmid-map-sequence-id-455374

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  24. p168-759

    Plasmid
    #62619
    Purpose
    ePathBrick pETM6 vector encoding CsF3H, AaDFR, DuLAR with Protein Scaffold GBD:SH3:PDZ (1:1:4)
    Depositor
    Mattheos Koffas
    Article
    Zhao et al Metab Eng. 2014 Dec 17;28C:43-53. doi: 1
    Inserts
    flavaone 3B-hydroxylase
    dihydroflavonol 4-reductase
    leucoanthocyanidin reductase
    Scaffold Protein encoding GBD:SH3:PDZ (1:1:4)
    Use
    Synthetic Biology
    Tags
    GBD, PDZ, and SH3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    62619-plasmid-map-sequence-id-103510

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  27. MT1X

    Plasmid
    #105709
    Purpose
    Bacterial expression plasmids for production of recombinant metallothionein 1x
    Depositor
    Artur Krężel
    Insert
    Metallothionein 1x (MT1X Human)
    Tags
    Chiting Binding Domanin-Intein
    Expression
    Bacterial
    Available Since
    April 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    105709-plasmid-map-sequence-id-203329

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  28. MT1F

    Plasmid
    #105703
    Purpose
    Bacterial expression plasmids for production of recombinant metallothionein 1f
    Depositor
    Artur Krężel
    Insert
    Metallothionein 1f (MT1F Human)
    Tags
    Chiting Binding Domanin-Intein
    Expression
    Bacterial
    Available Since
    April 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    105703-plasmid-map-sequence-id-203322

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  30. pWJ68

    Plasmid
    #46571
    Purpose
    Same as pdCas9, but with the w-dCas9 fusion (w is fused at the N-terminal end of dCas9)
    Depositor
    Luciano Marraffini
    Article
    Bikard et al Nucleic Acids Res. 2013 Jun 12.
    Inserts
    tracrRNA
    w-dcas9
    CRISPR array
    Use
    CRISPR
    Tags
    rpoZ
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Available Since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    46571-plasmid-map-sequence-id-65847

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  33. ibpAB-GFP

    Plasmid
    #109390
    Purpose
    GFP cassette under the control of the ibpAB sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.
    Depositor
    Tom Ellis
    Article
    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463
    Insert
    pibpAB-GFP
    Expression
    Bacterial
    Available Since
    May 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    109390-plasmid-map-sequence-id-210782

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  34. pET28a-HIS-Xyl-Xmod-DocIII

    Plasmid
    #60865
    Purpose
    Characteristic fingerprint molecule fused to dockerin type III domain to perform single molecule force spectroscopy experiments; covalently immobilized via introduced Cystein in Xylanase T120C
    Depositor
    Hermann Gaub
    Article
    Schoeler et al Nat Commun. 2014 Dec 8;5:5635. doi: 10.1
    Inserts
    Xyl
    Xmod-DocIII
    Tags
    6xHIS
    Expression
    Bacterial
    Mutation
    Threonine 120 to Cysteine in Xylanase domain
    Promoter
    T7
    Available Since
    Nov. 26, 2014
    Availability
    Academic Institutions and Nonprofits only
    60865-plasmid-map-sequence-id-98100

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  39. pXW117As(RS-E11-RinA)2

    Plasmid
    #123146
    Purpose
    Arsenic sensor with three-layered amplifier cascade. Need to work with plasmid pXWPrinA-gfp2. pSB4A3 carrying J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-33rinA-t
    Depositor
    Baojun Wang
    Article
    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d
    Insert
    J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-33rinA-t
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    J117, ParsR, PhrpLE, Pecf11
    Available Since
    May 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    123146-plasmid-map-sequence-id-243978

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Showing: 6921 - 6960 of 31059 results